PREFIX obo: <http://purl.obolibrary.org/obo/>
PREFIX rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#>
PREFIX rdfs: <http://www.w3.org/2000/01/rdf-schema#>
PREFIX xsd: <http://www.w3.org/2001/XMLSchema#>

obo:ECO_0005674 a rdfs:Class ;
  rdfs:label "ad-hoc qualitative phenotype observation evidence used in manual assertion" ;
  rdfs:comment "A type of ad-hoc qualitative phenotype observation that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005673 , obo:ECO_0007634 .

obo:OBI_0000312 a rdf:Property ;
  rdfs:label "is_specified_output_of"@en ;
  rdfs:comment "A relation between a planned process and a continuant participating in that process. The presence of the continuant at the end of the process is explicitly specified in the objective specification which the process realizes the concretization of."@en ;
  rdfs:domain obo:BFO_0000002 ;
  rdfs:range obo:OBI_0000011 ;
  rdfs:subPropertyOf obo:RO_0000056 .

obo:OBI_0001938 a rdf:Property ;
  rdfs:label "has value specification" ;
  rdfs:comment "A relation between an information content entity and a value specification that specifies its value." ;
  rdfs:domain obo:IAO_0000030 ;
  rdfs:range obo:OBI_0001933 ;
  rdfs:subPropertyOf obo:BFO_0000051 .

obo:ECO_9000000 a rdf:Property ;
  rdfs:label "used_in" ;
  rdfs:comment "A relation connecting an evidence type to an assertion method where the assertion method describes the type of process employed to evaluate the evidence." ;
  rdfs:domain obo:ECO_0000000 ;
  rdfs:range obo:ECO_0000217 .

obo:RO_0000056 a rdf:Property ;
  rdfs:label "participates in"@en ;
  rdfs:comment "a relation between a continuant and a process, in which the continuant is somehow involved in the process"@en ;
  rdfs:domain obo:BFO_0000002 ;
  rdfs:range obo:BFO_0000003 .

obo:IAO_0000136 a rdf:Property ;
  rdfs:label "is about"@en ;
  rdfs:comment "A (currently) primitive relation that relates an information artifact to an entity."@en ;
  rdfs:domain obo:IAO_0000030 ;
  rdfs:range <https://w3id.org/linkml/Any> .

obo:RO_0000058 a rdf:Property ;
  rdfs:label "is concretized as"@en ;
  rdfs:comment "A relationship between a generically dependent continuant and a specifically dependent continuant, in which the generically dependent continuant depends on some independent continuant in virtue of the fact that the specifically dependent continuant also depends on that same independent continuant. A generically dependent continuant may be concretized as multiple specifically dependent continuants."@en ;
  rdfs:domain obo:BFO_0000031 ;
  rdfs:range obo:BFO_0000020 .

obo:ECO_0007328 a rdfs:Class ;
  rdfs:label "biological aspect of ancestor evidence used in automatic assertion" ;
  rdfs:comment "A type of biological aspect of ancestor evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000308 , obo:ECO_0007826 .

obo:GO_0005515 a rdfs:Class ;
  rdfs:label "protein binding" ;
  rdfs:subClassOf obo:GO_0005488 .

obo:BFO_0000062 a rdf:Property ;
  rdfs:label "preceded by"@en ;
  rdfs:domain obo:BFO_0000003 ;
  rdfs:range obo:BFO_0000003 .

obo:BFO_0000063 a rdf:Property ;
  rdfs:label "precedes"@en ;
  rdfs:domain obo:BFO_0000003 ;
  rdfs:range obo:BFO_0000003 ;
  rdfs:subPropertyOf obo:RO_0002222 .

obo:OBI_1110060 a rdf:Property ;
  rdfs:label "process is result of" ;
  rdfs:comment "is a relationship between a process and a preceding occurrent that directly caused the later one to occur" ;
  rdfs:domain obo:BFO_0000003 ;
  rdfs:range obo:BFO_0000003 ;
  rdfs:subPropertyOf obo:RO_0002087 .

obo:BFO_0000055 a rdf:Property ;
  rdfs:label "realizes"@en ;
  rdfs:domain obo:BFO_0000015 ;
  rdfs:range obo:BFO_0000017 .

obo:RO_0000057 a rdf:Property ;
  rdfs:label "has participant"@en ;
  rdfs:comment "a relation between a process and a continuant, in which the continuant is somehow involved in the process"@en ;
  rdfs:domain obo:BFO_0000003 ;
  rdfs:range obo:BFO_0000002 .

obo:ECO_0001194 a rdfs:Class ;
  rdfs:label "in vitro demethylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro demethylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001062 .

obo:ECO_0007165 a rdfs:Class ;
  rdfs:label "frameshift mutation phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of frameshift mutation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0005512 .

obo:ECO_0007527 a rdfs:Class ;
  rdfs:label "xylE reporter gene assay evidence used in automatic assertion" ;
  rdfs:comment "A type of xylE reporter gene assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005537 , obo:ECO_0007765 .

obo:ECO_0008140 a rdfs:Class ;
  rdfs:label "antibody LB3.1-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody LB3.1-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008065 , obo:ECO_0008088 .

obo:OBI_0000681 a rdfs:Class ;
  rdfs:label "separation into different composition objective" ;
  rdfs:comment "A material separation objective aiming to separate a material entity that has parts of different types, and end with at least one output that is a material with parts of fewer types (modulo impurities)." ;
  rdfs:subClassOf obo:OBI_0000639 .

obo:OBI_0000833 a rdf:Property ;
  rdfs:label "objective_achieved_by" ;
  rdfs:comment "This relation obtains between an objective specification and a planned process when the criteria specified in the objective specification are met at the end of the planned process." ;
  rdfs:domain obo:IAO_0000005 ;
  rdfs:range obo:OBI_0000011 .

obo:GO_0110165 a rdfs:Class ;
  rdfs:label "cellular anatomical entity" ;
  rdfs:subClassOf obo:GO_0005575 .

obo:OBI_1110119 a rdf:Property ;
  rdfs:label "bound_to" ;
  rdfs:comment "A relationship between two material entities that form a complex based on a selective, non-covalent interaction." ;
  rdfs:domain obo:BFO_0000040 ;
  rdfs:range obo:BFO_0000040 .

obo:RO_0001000 a rdf:Property ;
  rdfs:label "derives from"@en ;
  rdfs:comment "a relation between two distinct material entities, the new entity and the old entity, in which the new entity begins to exist when the old entity ceases to exist, and the new entity inherits the significant portion of the matter of the old entity"@en ;
  rdfs:domain obo:BFO_0000004 ;
  rdfs:range obo:BFO_0000004 , obo:CL_0000236 .

obo:RO_0000087 a rdf:Property ;
  rdfs:label "has role"@en ;
  rdfs:comment "a relation between an independent continuant (the bearer) and a role, in which the role specifically depends on the bearer for its existence"@en ;
  rdfs:domain obo:BFO_0000004 ;
  rdfs:range obo:BFO_0000023 ;
  rdfs:subPropertyOf obo:RO_0000053 .

obo:RO_0000085 a rdf:Property ;
  rdfs:label "has function"@en ;
  rdfs:comment "a relation between an independent continuant (the bearer) and a function, in which the function specifically depends on the bearer for its existence"@en ;
  rdfs:domain obo:BFO_0000004 ;
  rdfs:range obo:BFO_0000034 ;
  rdfs:subPropertyOf obo:RO_0000053 .

obo:ECO_0008021 a rdfs:Class ;
  rdfs:label "string-matching method evidence used in automatic assertion" ;
  rdfs:comment "A type of string-matching method evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007669 , obo:ECO_0008011 .

obo:ECO_0000150 a rdfs:Class ;
  rdfs:label "in vitro transcription reconstitution assay evidence" ;
  rdfs:comment "A type of reconstitution assay evidence resulting from the use of reconstituted polymerase transcription systems to investigate RNA synthesis." ;
  rdfs:subClassOf obo:ECO_0000003 .

obo:OBI_0000891 a rdfs:Class ;
  rdfs:label "cell proliferation assay" ;
  rdfs:comment "A cytometry assay which measures the degreee to which input cells are replicating." ;
  rdfs:subClassOf obo:OBI_0001977 .

obo:OBI_0000299 a rdf:Property ;
  rdfs:label "has_specified_output"@en ;
  rdfs:comment "The inverse property of is_specified_output_of"@en ;
  rdfs:domain obo:OBI_0000011 ;
  rdfs:range obo:BFO_0000002 ;
  rdfs:subPropertyOf obo:RO_0000057 .

obo:OBI_0000417 a rdf:Property ;
  rdfs:label "achieves_planned_objective" ;
  rdfs:comment "This relation obtains between a planned process and a objective specification when the criteria specified in the objective specification are met at the end of the planned process." ;
  rdfs:domain obo:OBI_0000011 ;
  rdfs:range obo:IAO_0000005 .

obo:OBI_0000293 a rdf:Property ;
  rdfs:label "has_specified_input"@en ;
  rdfs:comment "The inverse property of is_specified_input_of"@en ;
  rdfs:domain obo:OBI_0000011 ;
  rdfs:range obo:BFO_0000002 , obo:BFO_0000040 , obo:IAO_0000027 ;
  rdfs:subPropertyOf obo:RO_0000057 .

obo:ECO_0006163 a rdfs:Class ;
  rdfs:label "nuclear magnetic resonance spectroscopy evidence" ;
  rdfs:comment "A type of nuclear magnetic resonance evidence used for quantification of metabolites or for the determination of chemical structure or composition." ;
  rdfs:subClassOf obo:ECO_0001105 .

obo:ECO_0006342 a rdfs:Class ;
  rdfs:label "qualitative macroscopy evidence" ;
  rdfs:comment "A type of macroscopy evidence in which non-numerical observed characteristics or features are captured as text descriptions or categorical assignments." ;
  rdfs:subClassOf obo:ECO_0001094 .

obo:OBI_0002620 a rdfs:Class ;
  rdfs:label "random mutagenesis" ;
  rdfs:comment "An induced mutation that produces a random mutation, either by introducing the organism to mutagens, or through a process such as error-prone PCR." ;
  rdfs:subClassOf obo:OBI_0001154 .

obo:ECO_0007057 a rdfs:Class ;
  rdfs:label "extended field laser confocal microscopy evidence" ;
  rdfs:comment "A type of confocal microscopy evidence that results in creation of a continuous confocal multi-colour mosaic from thousands of individually captured images." ;
  rdfs:subClassOf obo:ECO_0005587 .

obo:ECO_0000277 a rdfs:Class ;
  rdfs:label "Nimblegen array evidence used in manual assertion" ;
  rdfs:comment "A type of evidence arising from a Nimblegen array experiment that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000105 , obo:ECO_0000273 .

obo:ECO_0000354 a rdfs:Class ;
  rdfs:label "gene neighbors evidence used in manual assertion" ;
  rdfs:comment "A type of gene neighbors evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000084 , obo:ECO_0000317 .

obo:ECO_0006223 a rdfs:Class ;
  rdfs:label "X-ray crystallography-based structural model with high relative B-factor values used in automatic assertion" ;
  rdfs:comment "A type of X-ray crystallography-based structural model with high relative B-factor values that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005671 , obo:ECO_0006188 .

obo:ECO_0001061 a rdfs:Class ;
  rdfs:label "in vitro defarnesylation assay evidence" ;
  rdfs:comment "A type of defarnesylation assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001549 .

obo:ECO_0001265 a rdfs:Class ;
  rdfs:label "tryptic phosphopeptide mapping assay evidence used in manual assertion" ;
  rdfs:comment "A type of tryptic phosphopeptide mapping assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001132 , obo:ECO_0001230 .

obo:ECO_0001200 a rdfs:Class ;
  rdfs:label "in vitro phosphatase assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro phosphatase assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001067 .

obo:ECO_0000016 a rdfs:Class ;
  rdfs:label "loss-of-function mutant phenotype evidence" ;
  rdfs:comment "A type of mutant phenotype evidence where a phenotype is associated with altered gene product which lacks the molecular function of the wild-type gene." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0005539 a rdfs:Class ;
  rdfs:label "obsolete computationally derived logical inference used in automatic assertion" .

obo:OBI_0000916 a rdfs:Class ;
  rdfs:label "flow cytometry assay" ;
  rdfs:comment "A cytometry assay in which an input cell population is put in solution, is passed by a laser, and optical sensors are used to detect scattering of the laser light and/or fluorescence of specific markers to count and characterize the particles in solution." ;
  rdfs:subClassOf obo:OBI_0001977 .

obo:BFO_0000051 a rdf:Property ;
  rdfs:label "has part" ;
  rdfs:comment "a core relation that holds between a whole and its part"@en ;
  rdfs:range <https://w3id.org/linkml/Any> , obo:ECO_0007672 , obo:ECO_0000006 .

obo:ECO_0000273 a rdfs:Class ;
  rdfs:label "cDNA to DNA expression microarray evidence used in manual assertion" ;
  rdfs:comment "A type of cDNA to DNA expression microarray evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000097 , obo:ECO_0000275 .

obo:ECO_0001845 a rdfs:Class ;
  rdfs:label "cell population optical density evidence" ;
  rdfs:comment "A type of cell-based assay evidence in which optical density is used to measure some aspect of a population of cells. Examples of aspects that can affect optical density measurements include, but are not limited to, cell number and cell shape." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:GO_0090303 a rdfs:Class ;
  rdfs:label "positive regulation of wound healing" ;
  rdfs:subClassOf obo:GO_0061041 , obo:GO_0065007 , obo:GO_1903036 .

obo:RO_0002213 a rdf:Property ;
  rdfs:label "positively regulates" ;
  rdfs:range <https://w3id.org/linkml/Any> ;
  rdfs:subPropertyOf obo:RO_0002211 .

obo:RO_0002211 a rdf:Property ;
  rdfs:label "regulates" ;
  rdfs:range <https://w3id.org/linkml/Any> .

obo:ECO_0000501 a rdfs:Class ;
  rdfs:label "evidence used in automatic assertion" ;
  rdfs:comment "A type of evidence that is used in an automatic assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/automatically-assigned-evidence-codes> ;
  rdfs:subClassOf obo:ECO_0000000 .

obo:OBI_0000094 a rdfs:Class ;
  rdfs:label "material processing"@en ;
  rdfs:comment "A planned process which results in physical changes in a specified input material"@en ;
  rdfs:subClassOf obo:OBI_0000011 .

obo:ECO_0006155 a rdfs:Class ;
  rdfs:label "documented statement evidence used in manual assertion" ;
  rdfs:comment "A type of documented statement evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000352 , obo:ECO_0006151 .

obo:GO_0005488 a rdfs:Class ;
  rdfs:label "binding" ;
  rdfs:subClassOf obo:GO_0003674 .

obo:ECO_0005668 a rdfs:Class ;
  rdfs:label "survival rate analysis evidence used in manual assertion" ;
  rdfs:comment "A type of survival rate analysis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001225 , obo:ECO_0001822 .

obo:GO_1904951 a rdfs:Class ;
  rdfs:label "positive regulation of establishment of protein localization" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_0070201 , obo:GO_1903829 .

obo:ECO_0007628 a rdfs:Class ;
  rdfs:label "zone of inhibition evidence used in automatic assertion" ;
  rdfs:comment "A type of zone of inhibition evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007083 , obo:ECO_0007778 .

obo:OBI_0002050 a rdfs:Class ;
  rdfs:label "3D molecular structure determination assay of a MHC:ligand complex" ;
  rdfs:comment "A 3D structure determination of bound molecular complex assay that characterizes the 3-dimensional structure of a MHC:ligand complex." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_0001145 , obo:OBI_0002075 .

obo:GO_0036094 a rdfs:Class ;
  rdfs:label "small molecule binding" ;
  rdfs:subClassOf obo:GO_0005488 .

obo:OBI_0400142 a rdfs:Class ;
  rdfs:label "power supply" ;
  rdfs:comment "A power supply is an device or part of a device that permits the required application of a defined electrical charge to an instrument. The power supply may permit the defined application of a given amount of current for a defined length of time." ;
  rdfs:subClassOf obo:OBI_0000968 .

obo:OBI_0000268 a rdfs:Class ;
  rdfs:label "organism feature identification objective"@en ;
  rdfs:comment "Organism_feature_identification_objective is a biological_feature_identification_objective role describing a study designed to examine or characterize a biological feature monitored at the level of the organism, e.g. height, weight, stage of development, stage of life cycle."@en ;
  rdfs:subClassOf obo:OBI_0000015 .

obo:ECO_0008151 a rdfs:Class ;
  rdfs:label "antibody PT85-A-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody PT85-A-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008071 , obo:ECO_0008087 .

obo:ECO_0007791 a rdfs:Class ;
  rdfs:label "deubiquitination assay evidence used in automatic assertion" ;
  rdfs:comment "A type of deubiquitination assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001552 , obo:ECO_0007307 .

obo:ECO_0006146 a rdfs:Class ;
  rdfs:label "density functional theory simulation evidence used in automatic assertion" ;
  rdfs:comment "A type of density functional theory simulation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006144 .

obo:ECO_0007263 a rdfs:Class ;
  rdfs:label "ATP bioluminescence assay evidence used in automatic assertion" ;
  rdfs:comment "A type of ATP bioluminescence assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005011 , obo:ECO_0007774 .

obo:ECO_0008098 a rdfs:Class ;
  rdfs:label "antibody 34-5-8S-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody 34-5-8S-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008044 , obo:ECO_0008088 .

obo:BFO_0000034 a rdfs:Class ;
  rdfs:label "function"@en ;
  rdfs:subClassOf obo:BFO_0000016 .

obo:RO_0000079 a rdf:Property ;
  rdfs:label "function of"@en ;
  rdfs:comment "a relation between a function and an independent continuant (the bearer), in which the function specifically depends on the bearer for its existence"@en ;
  rdfs:domain obo:BFO_0000034 ;
  rdfs:range <https://w3id.org/linkml/Any> ;
  rdfs:subPropertyOf obo:RO_0000052 .

obo:BFO_0000054 a rdf:Property ;
  rdfs:label "realized in"@en ;
  rdfs:domain obo:BFO_0000017 ;
  rdfs:range obo:BFO_0000015 , obo:OBI_0000070 , obo:OBI_1110021 , obo:OBI_0000685 , obo:OBI_0001007 , obo:OBI_0600064 , obo:OBI_0000274 , obo:OBI_0000415 , obo:OBI_0000471 , obo:OBI_1110014 .

obo:RO_0000059 a rdf:Property ;
  rdfs:label "concretizes"@en ;
  rdfs:comment "A relationship between a specifically dependent continuant and a generically dependent continuant, in which the generically dependent continuant depends on some independent continuant in virtue of the fact that the specifically dependent continuant also depends on that same independent continuant. Multiple specifically dependent continuants can concretize the same generically dependent continuant."@en ;
  rdfs:domain obo:BFO_0000020 ;
  rdfs:range obo:BFO_0000031 .

obo:ECO_0007388 a rdfs:Class ;
  rdfs:label "gel-filtration evidence used in automatic assertion" ;
  rdfs:comment "A type of gel-filtration evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001049 , obo:ECO_0007681 .

obo:ECO_0006193 a rdfs:Class ;
  rdfs:label "differential scanning calorimetry evidence" ;
  rdfs:comment "A type of heat capacity-based evidence where the heat required to increase the temperature of the sample is continuously monitored and compared to the heat required to increase the temperature of a reference (having a largely identical composition compared to the sample, such as the empty buffer compared to a protein solution) over a range of temperatures." ;
  rdfs:subClassOf obo:ECO_0006192 .

obo:ECO_0006358 a rdfs:Class ;
  rdfs:label "differential scanning fluorimetry evidence used in manual assertion" ;
  rdfs:comment "A type of differential scanning fluorimetry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001249 , obo:ECO_0006301 , obo:ECO_0006334 .

obo:ECO_0007272 a rdfs:Class ;
  rdfs:label "translocation mutation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of translocation mutation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005515 , obo:ECO_0007325 .

obo:ECO_0006382 a rdfs:Class ;
  rdfs:label "sexual sporulation assay evidence used in manual assertion" ;
  rdfs:comment "A type of sexual sporulation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006346 , obo:ECO_0006378 .

obo:BFO_0000017 a rdfs:Class ;
  rdfs:label "realizable entity"@en ;
  rdfs:comment "A specifically dependent continuant  that inheres in continuant  entities and are not exhibited in full at every time in which it inheres in an entity or group of entities. The exhibition or actualization of a realizable entity is a particular manifestation, functioning or process that occurs under certain circumstances."@en ;
  rdfs:subClassOf obo:BFO_0000020 .

obo:OBI_0000432 a rdfs:Class ;
  rdfs:label "polyacrylamide gel"@en ;
  rdfs:comment "a material entity resulting from the polymerization of acrylamide with TEMED in some buffer solution"@en ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:ECO_0006263 a rdfs:Class ;
  rdfs:label "turbidity measurement evidence" ;
  rdfs:comment "A type of direct assay evidence where the optical properties of a solution is measured in the visible light spectrum, assessing the haziness or cloudiness as a result of the properties of the solutes or the changes in their material states." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0006338 a rdfs:Class ;
  rdfs:label "temperature jump relaxation evidence" ;
  rdfs:comment "A type of fluorescence evidence where the rates of fast chemical reactions in a solution are measured by the intensity of light absorbed or fluoresced by allowing the reaction mixture to come to an equilibrium, and then perturbing the equilibrium by a rapid temperature rise followed by relaxation of the system to the new equilibrium." ;
  rdfs:subClassOf obo:ECO_0001115 .

obo:ECO_0007714 a rdfs:Class ;
  rdfs:label "demethylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of demethylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001550 .

obo:ECO_0007447 a rdfs:Class ;
  rdfs:label "physical examination evidence used in automatic assertion" ;
  rdfs:comment "A type of physical examination evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001111 , obo:ECO_0007307 .

obo:UBERON_0000465 a rdfs:Class ;
  rdfs:label "material anatomical entity" ;
  rdfs:comment "Anatomical entity that has mass." ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:ECO_0008069 a rdfs:Class ;
  rdfs:label "antibody MKD6-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody MKD6 to determine that the H2-IAd protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:OBI_0003146 a rdfs:Class ;
  rdfs:label "assay measuring quantitative binding of an antigen:antibody complex" ;
  rdfs:comment "An assay that detects the binding of an antigen with an antibody, and produces a quantitative measurement of the binding as an output." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_1110127 .

obo:ECO_0006204 a rdfs:Class ;
  rdfs:label "far-UV circular dichroism evidence used in manual assertion" ;
  rdfs:comment "A type of far-UV circular dichroism evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006179 , obo:ECO_0006200 .

obo:ECO_0007311 a rdfs:Class ;
  rdfs:label "heterologous protein expression evidence used in automatic assertion" ;
  rdfs:comment "A type of heterologous protein expression evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000154 , obo:ECO_0007309 .

obo:GO_0140640 a rdfs:Class ;
  rdfs:label "catalytic activity, acting on a nucleic acid" ;
  rdfs:subClassOf obo:GO_0003824 .

obo:ECO_0006012 a rdfs:Class ;
  rdfs:label "patch-clamp recording evidence" ;
  rdfs:comment "A type of electrophysiology assay evidence in which a glass micropipette is sealed to the surface of a cell membrane (patch) to study ion channels." ;
  rdfs:subClassOf obo:ECO_0000164 .

obo:ECO_0006139 a rdfs:Class ;
  rdfs:label "molecular mechanics simulation evidence used in automatic assertion" ;
  rdfs:comment "A type of molecular mechanics simulation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006138 .

obo:ECO_0006331 a rdfs:Class ;
  rdfs:label "Congo red staining evidence" ;
  rdfs:comment "A type of staining evidence where Congo red is used to stain cellular structures including amyloid fibrils and aggregates, cell walls of plants and fungi, and for the outer membrane of Gram-negative bacteria." ;
  rdfs:subClassOf obo:ECO_0005019 .

obo:ECO_0007499 a rdfs:Class ;
  rdfs:label "alkaline phosphatase reporter gene assay evidence used in automatic assertion" ;
  rdfs:comment "A type of alkaline phosphatase reporter gene assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001801 , obo:ECO_0007765 .

obo:ECO_0001162 a rdfs:Class ;
  rdfs:label "co-electrophoresis evidence used in manual assertion" ;
  rdfs:comment "A type of co-electrophoresis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001025 .

obo:OBI_0000371 a rdfs:Class ;
  rdfs:label "heat function"@en ;
  rdfs:comment "A heat function is a function that increases the internal kinetic energy of a material"@en ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:ECO_0000264 a rdfs:Class ;
  rdfs:label "transmembrane domain prediction evidence used in manual assertion" ;
  rdfs:comment "A type of transmembrane domain prediction that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000083 , obo:ECO_0000257 .

obo:ECO_0007644 a rdfs:Class ;
  rdfs:label "curator inference from MedlinePlus dictionary" ;
  rdfs:comment "A type of curator inference from dictionary in which the reference is to an entry in the National Library of Medicine's MedLinePlus dictionary." ;
  rdfs:subClassOf obo:ECO_0007641 .

obo:ECO_0007675 a rdfs:Class ;
  rdfs:label "manually integrated combinatorial evidence used in manual assertion" ;
  rdfs:comment "A type of manually integrated combinatorial evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000244 , obo:ECO_0007674 .

obo:ECO_0005620 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation-PCR evidence used in manual assertion" ;
  rdfs:comment "A type of chromatin immunoprecipitation-PCR evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000227 , obo:ECO_0005644 .

obo:ECO_0007440 a rdfs:Class ;
  rdfs:label "natural variation mutant evidence used in automatic assertion" ;
  rdfs:comment "A type of natural variation mutant evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001103 , obo:ECO_0007635 .

obo:ECO_0006371 a rdfs:Class ;
  rdfs:label "structural ensemble generation evidence used in automatic assertion" ;
  rdfs:comment "A type of structural ensemble generation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006339 , obo:ECO_0006369 .

obo:ECO_0007652 a rdfs:Class ;
  rdfs:label "automatically integrated combinatorial computational evidence used in manual assertion" ;
  rdfs:comment "A type of automatically integrated combinatorial computational evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000245 , obo:ECO_0007651 , obo:ECO_0007678 .

obo:ECO_0001830 a rdfs:Class ;
  rdfs:label "host colonization assay evidence" ;
  rdfs:comment "A type of experimental evidence in which the colonization or adhesion capacity is measured." ;
  rdfs:subClassOf obo:ECO_0000006 .

obo:OBI_0001593 a rdfs:Class ;
  rdfs:label "competitive inhibition of binding assay" ;
  rdfs:comment "A binding assay that detects the inhibition of binding between 2 material entities known to form a complex by the addition of a third material entity of interest. Inhibition of binding between the 2 materials reflects binding by the third material." ;
  rdfs:subClassOf obo:OBI_0001146 .

obo:ECO_0005507 a rdfs:Class ;
  rdfs:label "nonsense mutation phenotypic evidence" ;
  rdfs:comment "A type of point mutation phenotypic evidence resulting in an incomplete and usually nonfunctional protein product because of a premature stop codon." ;
  rdfs:subClassOf obo:ECO_0001113 .

obo:ECO_0001173 a rdfs:Class ;
  rdfs:label "cytochrome C release assay evidence used in manual assertion" ;
  rdfs:comment "A type of cytochrome C release assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001036 , obo:ECO_0007698 .

obo:PATO_0001421 a rdfs:Class ;
  rdfs:label "alive" ;
  rdfs:comment "A viability quality inhering in a bearer by virtue of the bearer's condition before death." ;
  rdfs:subClassOf obo:PATO_0000169 .

obo:ECO_0005800 a rdfs:Class ;
  rdfs:label "dilution assay evidence"@en ;
  rdfs:comment "A type of direct assay evidence where the activity of a solution is determined by exposing an indicator culture or organism to a series of dilutions and determining the lowest concentration that elicits a biological response." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:IAO_0000009 a rdfs:Class ;
  rdfs:label "datum label"@en ;
  rdfs:comment "A label is a symbol that is part of some other datum and is used to either partially define  the denotation of that datum or to provide a means for identifying the datum as a member of the set of data with the same label"@en ;
  rdfs:subClassOf obo:IAO_0000030 .

obo:ECO_0007086 a rdfs:Class ;
  rdfs:label "Etest evidence used in manual assertion" ;
  rdfs:comment "A type of Etest evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007084 , obo:ECO_0007085 .

obo:ECO_0007135 a rdfs:Class ;
  rdfs:label "3C-seq evidence used in manual assertion" ;
  rdfs:comment "A type of 3C-seq evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000239 , obo:ECO_0000269 .

obo:ECO_0007361 a rdfs:Class ;
  rdfs:label "co-electrophoresis evidence used in automatic assertion" ;
  rdfs:comment "A type of co-electrophoresis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001025 , obo:ECO_0007769 .

obo:ECO_0000035 a rdfs:Class ;
  rdfs:label "no evidence data found" ;
  rdfs:comment "A type of curator inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources." ;
  rdfs:subClassOf obo:ECO_0000205 .

obo:ECO_0001591 a rdfs:Class ;
  rdfs:label "atomic force microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of atomic force microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001232 , obo:ECO_0001589 .

obo:GO_0070727 a rdfs:Class ;
  rdfs:label "cellular macromolecule localization" ;
  rdfs:subClassOf obo:GO_0033036 , obo:GO_0051641 .

obo:GO_0032549 a rdfs:Class ;
  rdfs:label "ribonucleoside binding" ;
  rdfs:subClassOf obo:GO_0001882 .

obo:ECO_0008135 a rdfs:Class ;
  rdfs:label "antibody IVD12-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody IVD12-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008063 , obo:ECO_0008087 .

obo:ECO_0007663 a rdfs:Class ;
  rdfs:label "manually integrated combinatorial computational and experimental evidence used in manual assertion" ;
  rdfs:comment "A type of manually integrated combinatorial computational and experimental evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007662 , obo:ECO_0007675 , obo:ECO_0007744 .

obo:GO_0043392 a rdfs:Class ;
  rdfs:label "negative regulation of DNA binding" ;
  rdfs:subClassOf obo:GO_0051100 , obo:GO_0051101 , obo:GO_0065007 .

obo:RO_0002212 a rdf:Property ;
  rdfs:label "negatively regulates" ;
  rdfs:range <https://w3id.org/linkml/Any> ;
  rdfs:subPropertyOf obo:RO_0002211 .

obo:ECO_0008107 a rdfs:Class ;
  rdfs:label "antibody B22-249.R1-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody B22-249.R1-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008049 , obo:ECO_0008087 .

obo:ECO_0007046 a rdfs:Class ;
  rdfs:label "scanning electron microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of scanning electron microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006003 , obo:ECO_0007045 .

obo:GO_0006305 a rdfs:Class ;
  rdfs:label "DNA alkylation" ;
  rdfs:subClassOf obo:GO_0006304 .

obo:ECO_0007227 a rdfs:Class ;
  rdfs:label "enzyme inhibition evidence used in automatic assertion" ;
  rdfs:comment "A type of enzyme inhibition evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000184 , obo:ECO_0007468 , obo:ECO_0007785 .

obo:GO_0001816 a rdfs:Class ;
  rdfs:label "cytokine production" ;
  rdfs:subClassOf obo:GO_0010467 , obo:GO_0032501 .

obo:GO_0022411 a rdfs:Class ;
  rdfs:label "cellular component disassembly" ;
  rdfs:subClassOf obo:GO_0016043 .

obo:GO_0033218 a rdfs:Class ;
  rdfs:label "amide binding" ;
  rdfs:subClassOf obo:GO_0005488 .

obo:ECO_0000066 a rdfs:Class ;
  rdfs:label "yeast one-hybrid evidence" ;
  rdfs:comment "A type of bait-prey hybrid interaction evidence that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors." ;
  rdfs:subClassOf obo:ECO_0000025 .

obo:OBI_0001331 a rdfs:Class ;
  rdfs:label "transcription profiling identification objective"@en ;
  rdfs:comment "A molecular feature identification objective that aims to characterize the abundance of transcripts"@en ;
  rdfs:subClassOf obo:OBI_0000131 .

obo:ECO_0005518 a rdfs:Class ;
  rdfs:label "RNA detection assay evidence" ;
  rdfs:comment "A type of molecule detection assay evidence resulting from the detection of specific RNAs." ;
  rdfs:subClassOf obo:ECO_0005516 , obo:IAO_0000030 .

obo:ECO_0006182 a rdfs:Class ;
  rdfs:label "small-angle X-ray scattering evidence" ;
  rdfs:comment "A type of light scattering evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight and structural transitions by irradiating the sample with an X-ray beam and the scattered intensity is probed at small angles (typically in the range of 0.1-10 degrees) by a detector." ;
  rdfs:subClassOf obo:ECO_0007061 .

obo:ECO_0001110 a rdfs:Class ;
  rdfs:label "peptide affinity enrichment evidence" ;
  rdfs:comment "A type of affinity evidence resulting from the appending of affinity tags on proteins so that they may be purified from their biological source using an affinity technique." ;
  rdfs:subClassOf obo:ECO_0000023 .

obo:ECO_0007613 a rdfs:Class ;
  rdfs:label "koehler illumination microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of koehler illumination microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007053 , obo:ECO_0007435 .

obo:ECO_0007248 a rdfs:Class ;
  rdfs:label "cap analysis of gene expression evidence used in automatic assertion" ;
  rdfs:comment "A type of cap analysis of gene expression evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000309 , obo:ECO_0007303 .

obo:ECO_0007038 a rdfs:Class ;
  rdfs:label "5-cyano-2,3-ditolyl tetrazolium chloride staining evidence used in manual assertion" ;
  rdfs:comment "A type of 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0007037 .

obo:ECO_0007146 a rdfs:Class ;
  rdfs:label "particle size and count assay evidence used in manual assertion" ;
  rdfs:comment "A type of particle size and count assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0000334 .

obo:GO_0043170 a rdfs:Class ;
  rdfs:label "macromolecule metabolic process" ;
  rdfs:subClassOf obo:GO_0071704 .

obo:ECO_0000342 a rdfs:Class ;
  rdfs:label "support of intron positions by RNA-sequencing alignment evidence" ;
  rdfs:comment "A type of RNA-seq evidence where intron locations in predicted transcripts are compared to intron locations supported by RNA-seq evidence." ;
  rdfs:subClassOf obo:ECO_0000295 .

obo:ECO_0007377 a rdfs:Class ;
  rdfs:label "cytochrome C release assay evidence used in automatic assertion" ;
  rdfs:comment "A type of cytochrome C release assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001036 , obo:ECO_0007777 .

obo:ECO_0008087 a rdfs:Class ;
  rdfs:label "antibody-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006076 , obo:ECO_0008039 .

obo:ECO_0006286 a rdfs:Class ;
  rdfs:label "magnetic resonance evidence used in automatic assertion" ;
  rdfs:comment "A type of magnetic resonance evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006252 , obo:ECO_0007297 .

obo:ECO_0007769 a rdfs:Class ;
  rdfs:label "gel electrophoresis evidence used in automatic assertion" ;
  rdfs:comment "A type of gel electrophoresis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000337 , obo:ECO_0007307 .

obo:ECO_0006274 a rdfs:Class ;
  rdfs:label "static fluorescence quenching evidence" ;
  rdfs:comment "A type of physical interaction evidence derived from the decrease (quenching) of fluorescence as a result of the intramolecular interaction between a fluorescence donor and acceptor creating a non-fluorescent ground state." ;
  rdfs:subClassOf obo:ECO_0000021 , obo:ECO_0001115 .

obo:ECO_0006226 a rdfs:Class ;
  rdfs:label "cryogenic electron microscopy-based structural model with high relative B-factor values used in manual assertion" ;
  rdfs:comment "A type of cryogenic electron microscopy-based structural model with high relative B-factor values that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006190 , obo:ECO_0006208 .

obo:ECO_0007252 a rdfs:Class ;
  rdfs:label "pulsed-field gel electrophoresis evidence used in automatic assertion" ;
  rdfs:comment "A type of pulsed-field gel electrophoresis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000338 , obo:ECO_0007769 .

obo:GO_0060147 a rdfs:Class ;
  rdfs:label "regulation of posttranscriptional gene silencing" ;
  rdfs:subClassOf obo:GO_0010468 , obo:GO_0065007 .

obo:GO_0046483 a rdfs:Class ;
  rdfs:label "heterocycle metabolic process" ;
  rdfs:subClassOf obo:GO_0044237 .

obo:ECO_0000331 a rdfs:Class ;
  rdfs:label "coding sequence splice pattern evidence used in automatic assertion" ;
  rdfs:comment "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000328 , obo:ECO_0007825 .

obo:ECO_0006393 a rdfs:Class ;
  rdfs:label "bait-prey interaction evidence used in automatic assertion" ;
  rdfs:comment "A type of bait-prey interaction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006351 , obo:ECO_0007373 .

obo:ECO_0000001 a rdfs:Class ;
  rdfs:label "inference from background scientific knowledge" ;
  rdfs:comment "A type of curator inference where conclusions are drawn based on the background scientific knowledge of the curator." ;
  rdfs:subClassOf obo:ECO_0000205 .

obo:ECO_0006001 a rdfs:Class ;
  rdfs:label "Cya fusion reporter assay evidence" ;
  rdfs:comment "A type of evidence derived from exploiting the CyaA (calmodulin-activated adenylate cyclase toxin) secreted by Bordatella pertussis to synthesize cAMP and alter cellular physiology in a host cell for various biological applications." ;
  rdfs:subClassOf obo:ECO_0000017 , obo:ECO_0000049 .

obo:ECO_0007666 a rdfs:Class ;
  rdfs:label "automatically integrated combinatorial computational and experimental evidence used in manual assertion" ;
  rdfs:comment "A type of automatically integrated combinatorial computational and experimental evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000245 , obo:ECO_0007665 , obo:ECO_0007744 .

obo:GO_0032270 a rdfs:Class ;
  rdfs:label "positive regulation of cellular protein metabolic process" ;
  rdfs:subClassOf obo:GO_0031325 , obo:GO_0032268 , obo:GO_0051247 , obo:GO_0065007 .

obo:ECO_0001236 a rdfs:Class ;
  rdfs:label "multiplex bead-based immunoassay evidence used in manual assertion" ;
  rdfs:comment "A type of multiplex bead-based immunoassay evidence that is  used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001102 .

obo:ECO_0001027 a rdfs:Class ;
  rdfs:label "colony counting evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from the counting of microbial colonies that arise from viable cells grown on a plate or dish." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:OBI_0000880 a rdfs:Class ;
  rdfs:label "RNA extract" ;
  rdfs:comment "an extract which is the output of an extraction process in which RNA molecules are isolated from a specimen." ;
  rdfs:subClassOf obo:OBI_0001010 .

obo:OBI_0000643 a rdf:Property ;
  rdfs:label "has grain" ;
  rdfs:comment "the relation of the cells in the finger of the skin to the finger, in which an indeterminate number of grains are parts of the whole by virtue of being grains in a collective that is part of the whole, and in which removing one granular part does not nec- essarily damage or diminish the whole. Ontological Whether there is a fixed, or nearly fixed number of parts - e.g. fingers of the hand, chambers of the heart, or wheels of a car - such that there can be a notion of a single one being missing, or whether, by contrast, the number of parts is indeterminate - e.g., cells in the skin of the hand, red cells in blood, or rubber molecules in the tread of the tire of the wheel of the car." ;
  rdfs:range <https://w3id.org/linkml/Any> , obo:CHEBI_23367 , obo:CL_0000010 ;
  rdfs:subPropertyOf obo:BFO_0000051 .

obo:OBI_0000832 a rdfs:Class ;
  rdfs:label "measurement device" ;
  rdfs:comment "A device in which a measure function inheres." ;
  rdfs:subClassOf obo:OBI_0000968 .

obo:ECO_0007205 a rdfs:Class ;
  rdfs:label "southern hybridization evidence used in automatic assertion" ;
  rdfs:comment "A type of southern hybridization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000078 , obo:ECO_0007817 .

obo:ECO_0006234 a rdfs:Class ;
  rdfs:label "selective antibody-based structural conformation evidence used in manual assertion" ;
  rdfs:comment "A type of selective antibody-based structural conformation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0006194 .

obo:ECO_0007501 a rdfs:Class ;
  rdfs:label "chloramphenicol acetyltransferase reporter gene assay evidence used in automatic assertion" ;
  rdfs:comment "A type of chloramphenicol acetyltransferase reporter gene assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001803 , obo:ECO_0007765 .

obo:GO_0051130 a rdfs:Class ;
  rdfs:label "positive regulation of cellular component organization" ;
  rdfs:subClassOf obo:GO_0048522 , obo:GO_0051128 , obo:GO_0065007 .

obo:OBI_0500010 a rdfs:Class ;
  rdfs:label "reference design" ;
  rdfs:comment "A reference experiment design type is where all samples are compared to a common reference." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:OBI_0002516 a rdfs:Class ;
  rdfs:label "brain specimen" ;
  rdfs:comment "A specimen that is derived from brain." ;
  rdfs:subClassOf obo:OBI_0001479 .

obo:GO_0032269 a rdfs:Class ;
  rdfs:label "negative regulation of cellular protein metabolic process" ;
  rdfs:subClassOf obo:GO_0031324 , obo:GO_0032268 , obo:GO_0051248 , obo:GO_0065007 .

obo:GO_0010243 a rdfs:Class ;
  rdfs:label "response to organonitrogen compound" ;
  rdfs:subClassOf obo:GO_0010033 , obo:GO_1901698 .

obo:ECO_0007693 a rdfs:Class ;
  rdfs:label "transcription assay evidence used in manual assertion" ;
  rdfs:comment "A type of transcription assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000168 , obo:ECO_0000314 .

obo:OBI_0001933 a rdfs:Class ;
  rdfs:label "value specification" ;
  rdfs:comment "An information content entity that specifies a value within a classification scheme or on a quantitative scale." ;
  rdfs:subClassOf obo:IAO_0000030 .

obo:OBI_0002135 a rdf:Property ;
  rdfs:label "has specified value"@en ;
  rdfs:comment "A relation between a value specification and a literal." ;
  rdfs:domain obo:OBI_0001933 ;
  rdfs:range <https://w3id.org/linkml/Any> .

obo:OBI_0001937 a rdf:Property ;
  rdfs:label "has specified numeric value" ;
  rdfs:comment "A relation between a value specification and a number that quantifies it." ;
  rdfs:domain obo:OBI_0001933 ;
  rdfs:range <http://www.w3.org/2002/07/owl#real> ;
  rdfs:subPropertyOf obo:OBI_0002135 .

obo:ECO_0007068 a rdfs:Class ;
  rdfs:label "colony papillation assay phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of colony papillation assay phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007067 , obo:ECO_0007078 .

obo:ECO_0008129 a rdfs:Class ;
  rdfs:label "antibody HB180-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody HB180-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008060 , obo:ECO_0008087 .

obo:ECO_0007360 a rdfs:Class ;
  rdfs:label "chimeric protein phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of chimeric protein phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001024 , obo:ECO_0007325 .

obo:ECO_0006027 a rdfs:Class ;
  rdfs:label "colony biofilm assay evidence used in manual assertion" ;
  rdfs:comment "A type of colony biofilm assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006021 , obo:ECO_0006026 .

obo:GO_0120034 a rdfs:Class ;
  rdfs:label "positive regulation of plasma membrane bounded cell projection assembly" ;
  rdfs:subClassOf obo:GO_0031346 , obo:GO_0044089 , obo:GO_0065007 , obo:GO_0120032 .

obo:ECO_0007549 a rdfs:Class ;
  rdfs:label "genetically encoded fluorescent sensor evidence used in automatic assertion" ;
  rdfs:comment "A type of genetically encoded fluorescent sensor evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006036 , obo:ECO_0007548 .

obo:ECO_0007526 a rdfs:Class ;
  rdfs:label "ultraviolet light footprinting evidence used in automatic assertion" ;
  rdfs:comment "A type of ultraviolet light footprinting evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001826 , obo:ECO_0007505 .

obo:ECO_0001809 a rdfs:Class ;
  rdfs:label "DNA affinity chromatography evidence" ;
  rdfs:comment "A type of chromatography evidence where immobilized promoter DNA is labeled and bound to a matrix through which a protein mixture is poured and then eluted to purify specific DNA binding proteins." ;
  rdfs:subClassOf obo:ECO_0000325 .

obo:ECO_0007821 a rdfs:Class ;
  rdfs:label "spectrometry evidence used in automatic assertion" ;
  rdfs:comment "A type of spectrometry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005504 , obo:ECO_0007297 .

obo:GO_0071417 a rdfs:Class ;
  rdfs:label "cellular response to organonitrogen compound" ;
  rdfs:subClassOf obo:GO_0010243 , obo:GO_0071310 , obo:GO_0071495 , obo:GO_1901699 .

obo:ECO_0006157 a rdfs:Class ;
  rdfs:label "self-reported individual's statement evidence used in manual assertion" ;
  rdfs:comment "A type of self-reported individual's statement evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006153 , obo:ECO_0006155 .

obo:ECO_0005636 a rdfs:Class ;
  rdfs:label "green fluorescent protein reporter gene assay evidence used in manual assertion" ;
  rdfs:comment "A type of green fluorescent protein reporter gene assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000270 , obo:ECO_0001816 .

obo:OBI_0001955 a rdfs:Class ;
  rdfs:label "competitive binding reference ligand role" ;
  rdfs:comment "A positive reference substance role that inheres in a material entity that is known to bind to a target entity, and that is realized in a competitive binding assay that has as specified input the target entity, an evaluant and the positive reference substance, where the binding of the evaluant to the target is measured based on the evaluant's ability to compete with the positive reference substance for binding to the target." ;
  rdfs:subClassOf obo:OBI_0000064 .

obo:RO_0000052 a rdf:Property ;
  rdfs:label "inheres in"@en ;
  rdfs:comment "a relation between a specifically dependent continuant (the dependent) and an independent continuant (the bearer), in which the dependent specifically depends on the bearer for its existence"@en ;
  rdfs:range <https://w3id.org/linkml/Any> .

obo:ECO_0007395 a rdfs:Class ;
  rdfs:label "in vitro deacetylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro deacetylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001060 , obo:ECO_0007771 .

obo:ECO_0000027 a rdfs:Class ;
  rdfs:label "structural similarity evidence" ;
  rdfs:comment "A type of similarity evidence based on structural similarity of an annotated gene or gene product to another gene or group of genes." ;
  rdfs:subClassOf obo:ECO_0000057 .

obo:ECO_0006144 a rdfs:Class ;
  rdfs:label "density functional theory simulation evidence" ;
  rdfs:comment "A type of quantum mechanics simulation evidence that results from the calculation of ground-state electronic structure of atoms, molecules and solid state materials." ;
  rdfs:subClassOf obo:ECO_0006141 .

obo:ECO_0001817 a rdfs:Class ;
  rdfs:label "glutathione S-transferase pull-down assay evidence" ;
  rdfs:comment "A type of bait-prey hybrid interaction evidence where a gene is fused with the GST gene and the resulting recombinant bait protein is captured on an immobilized Glutathione affinity ligand and incubated with prey protein to identify and characterize protein-protein interactions." ;
  rdfs:subClassOf obo:ECO_0000025 .

obo:ECO_0006353 a rdfs:Class ;
  rdfs:label "Congo red staining evidence used in automatic assertion" ;
  rdfs:comment "A type of Congo red staining evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006331 , obo:ECO_0007798 .

obo:ECO_0008079 a rdfs:Class ;
  rdfs:label "specific MHC protein expression-based MHC presentation evidence" ;
  rdfs:comment "A type of protein expression-based MHC presentation evidence that employs knowledge of a single specific MHC molecule's presence to determine that a specific MHC molecule presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008078 .

obo:ECO_0001211 a rdfs:Class ;
  rdfs:label "in vivo demethylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo demethylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001078 .

obo:CHEBI_28619 a rdfs:Class ;
  rdfs:label "acrylamide" ;
  rdfs:comment "A member of the class of acrylamides that results from the formal condensation of acrylic acid with ammonia." ;
  rdfs:subClassOf obo:CHEBI_23367 .

obo:ECO_0000146 a rdfs:Class ;
  rdfs:label "Northwestern blot evidence" ;
  rdfs:comment "A type of nucleic acid binding evidence in which RNA-protein binding is detected using labeled RNA as probes, hybridized to electrophoretically separated proteins." ;
  rdfs:subClassOf obo:ECO_0000136 .

obo:GO_0001067 a rdfs:Class ;
  rdfs:label "transcription regulatory region nucleic acid binding" ;
  rdfs:subClassOf obo:GO_0003676 .

obo:GO_0045926 a rdfs:Class ;
  rdfs:label "negative regulation of growth" ;
  rdfs:subClassOf obo:GO_0040008 , obo:GO_0048519 , obo:GO_0065007 .

obo:ECO_0007049 a rdfs:Class ;
  rdfs:label "phase contrast microscopy evidence" ;
  rdfs:comment "A type of microscopy evidence where a transparent specimen is illuminated with visible light and small phase shifts in the light passing through the specimen are used to produce an image." ;
  rdfs:subClassOf obo:ECO_0001098 .

obo:ECO_0007009 a rdfs:Class ;
  rdfs:label "radioligand binding assay evidence used in manual assertion" ;
  rdfs:comment "A type of radioligand binding assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006076 , obo:ECO_0007008 .

obo:GO_0015908 a rdfs:Class ;
  rdfs:label "fatty acid transport" ;
  rdfs:subClassOf obo:GO_0006869 , obo:GO_0015849 .

obo:BFO_0000050 a rdf:Property ;
  rdfs:label "part of" ;
  rdfs:comment "a core relation that holds between a part and its whole"@en ;
  rdfs:range <https://w3id.org/linkml/Any> .

obo:ECO_0001097 a rdfs:Class ;
  rdfs:label "medical imaging evidence" ;
  rdfs:comment "A type of imaging assay evidence resulting from the use of technology to visualize and provide information about the body to diagnose, treat, or monitor medical conditions." ;
  rdfs:subClassOf obo:ECO_0000324 .

obo:ECO_0007075 a rdfs:Class ;
  rdfs:label "phenomic profiling assay evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence that results from the comparison of phenotype profiles (the physical and biochemical traits of organisms in particular environments) across multiple strains; that is, a meta-analysis of a set of phenotypic patterns from many tests in response to a series of changing genetic and/or environmental factors." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0001043 a rdfs:Class ;
  rdfs:label "Edman degradation evidence used in manual assertion" ;
  rdfs:comment "A type of Edman degradation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001044 .

obo:ECO_0001171 a rdfs:Class ;
  rdfs:label "crystallography evidence used in manual assertion" ;
  rdfs:comment "A type of crystallography evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001034 .

obo:ECO_0007128 a rdfs:Class ;
  rdfs:label "chain termination sequencing evidence used in manual assertion" ;
  rdfs:comment "A type of chain termination sequencing evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000225 , obo:ECO_0000269 .

obo:ECO_0005517 a rdfs:Class ;
  rdfs:label "protein detection assay evidence" ;
  rdfs:comment "A type of molecule detection assay evidence resulting from the detection and quantification of a protein, or multiple proteins." ;
  rdfs:subClassOf obo:ECO_0005516 .

obo:OBI_0000716 a rdfs:Class ;
  rdfs:label "ChIP-seq assay" ;
  rdfs:comment "An assay in which chromatin is immunoprecipitated and subsequently analyzed using a DNA sequencing step to identify which parts of DNA are part of the isolated chromatin" ;
  rdfs:subClassOf obo:OBI_0001954 .

obo:ECO_0007806 a rdfs:Class ;
  rdfs:label "localization evidence used in automatic assertion" ;
  rdfs:comment "A type of localization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001533 , obo:ECO_0007307 .

obo:ECO_0007421 a rdfs:Class ;
  rdfs:label "in vivo protein kinase assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo protein kinase assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001085 , obo:ECO_0007767 .

obo:ECO_0008188 a rdfs:Class ;
  rdfs:label "protein proximity labeling assay evidence used in manual assertion" ;
  rdfs:comment "A type of protein proximity labeling assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0008183 .

obo:OBI_1110093 a rdfs:Class ;
  rdfs:label "disposition to infect an organism"@en ;
  rdfs:comment "Is a role borne by an agent, and realized when in contact with or inside another organism in which it is capable of replicating and causing disease"@en ;
  rdfs:subClassOf obo:BFO_0000016 .

obo:ECO_0007720 a rdfs:Class ;
  rdfs:label "desumoylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of desumoylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001551 .

obo:GO_0005694 a rdfs:Class ;
  rdfs:label "chromosome" ;
  rdfs:comment "A structure composed of a very long molecule of DNA and associated proteins (e.g. histones) that carries hereditary information." ;
  rdfs:subClassOf obo:GO_0005575 .

obo:ECO_0000302 a rdfs:Class ;
  rdfs:label "author statement used in manual assertion" ;
  rdfs:comment "A type of author statement that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000204 , obo:ECO_0006155 .

obo:ECO_0007035 a rdfs:Class ;
  rdfs:label "copper transport assay evidence" ;
  rdfs:comment "A type of transport assay evidence where the rate of copper transport is estimated." ;
  rdfs:subClassOf obo:ECO_0000134 .

obo:ECO_0008162 a rdfs:Class ;
  rdfs:label "antibody Y-3-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody Y-3-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008076 , obo:ECO_0008088 .

obo:ECO_0007434 a rdfs:Class ;
  rdfs:label "medical imaging evidence used in automatic assertion" ;
  rdfs:comment "A type of medical imaging evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001097 , obo:ECO_0007363 .

obo:ECO_0000172 a rdfs:Class ;
  rdfs:label "biochemical trait analysis evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence in which the biochemical aspect of a mutation is analyzed." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0007154 a rdfs:Class ;
  rdfs:label "differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) used in manual assertion" ;
  rdfs:comment "A type of differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000291 , obo:ECO_0000358 .

obo:ECO_0001806 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation- exonuclease evidence" ;
  rdfs:comment "A type of chromatin immunoprecipitation evidence that uses gama-exonuclease to digest TF-unbound DNA after ChIP for the identification of transcription factor binding site locations with high-resolution data." ;
  rdfs:subClassOf obo:ECO_0000226 .

obo:OBI_0600055 a rdfs:Class ;
  rdfs:label "DNA restriction enzyme digestion"@en ;
  rdfs:comment "A specific enzymatic cleavage of DNA molecules using endonucleases that cleave DNA molecules at specific sites based on the surrounding nucleotide sequence"@en ;
  rdfs:subClassOf obo:OBI_0600050 .

obo:ECO_0008177 a rdfs:Class ;
  rdfs:label "MHC-specific blocking based MHC restriction evidence used in manual assertion" ;
  rdfs:comment "A type of MHC-specific blocking based MHC restriction evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006076 , obo:ECO_0008084 .

obo:GO_1904798 a rdfs:Class ;
  rdfs:label "positive regulation of core promoter binding" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_1904796 , obo:GO_2000679 .

obo:ECO_0006360 a rdfs:Class ;
  rdfs:label "fluorescence correlation spectroscopy evidence used in manual assertion" ;
  rdfs:comment "A type of fluorescence correlation spectroscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001249 , obo:ECO_0006335 .

obo:OBI_0001634 a rdfs:Class ;
  rdfs:label "calorimetric binding assay" ;
  rdfs:comment "A binding assay in which the heat generated or absorbed during a binding event is measured, which allows determination of binding constants, reaction stoichiometry, enthalpy and entropy." ;
  rdfs:subClassOf obo:OBI_0001146 .

obo:ECO_0000251 a rdfs:Class ;
  rdfs:label "similarity evidence used in automatic assertion" ;
  rdfs:comment "A type of similarity evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000041 , obo:ECO_0000501 .

obo:ECO_0006289 a rdfs:Class ;
  rdfs:label "site-directed spin-labelling electron paramagnetic resonance evidence used in manual assertion" ;
  rdfs:comment "A type of site-directed spin-labelling electron paramagnetic resonance evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006254 , obo:ECO_0006287 .

obo:OBI_00001975 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation"@en ;
  rdfs:comment "An immunoprecipitation in which chromatin (i.e. packaged DNA which can include protein and RNA complexes) is cut into short regions, reversibly cross linked, and antibodies or tags are used to select for pieces of chromatin with desired characteristics."@en ;
  rdfs:subClassOf obo:OBI_0000690 .

obo:ECO_0007789 a rdfs:Class ;
  rdfs:label "transport assay evidence used in automatic assertion" ;
  rdfs:comment "A type of transport assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000134 , obo:ECO_0007307 .

obo:ECO_0007756 a rdfs:Class ;
  rdfs:label "curator inference from Britannica used in manual assertion" ;
  rdfs:comment "A type of curator inference from Britannica that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007639 , obo:ECO_0007753 .

obo:ECO_0008050 a rdfs:Class ;
  rdfs:label "antibody B7/21-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody B7/21 to determine that the HLA-DP protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0007735 a rdfs:Class ;
  rdfs:label "structure determination evidence used in manual assertion" ;
  rdfs:comment "A type of structure determination evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0005031 .

obo:ECO_0006320 a rdfs:Class ;
  rdfs:label "cell aggregation evidence used in automatic assertion" ;
  rdfs:comment "A type of cell aggregation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006269 , obo:ECO_0007773 .

obo:ECO_0006185 a rdfs:Class ;
  rdfs:label "author inference" ;
  rdfs:comment "A type of inferential evidence where an assertion is derived by the authors of a scientific publication from another assertion and/or primary data via logical inference or other rational means." ;
  rdfs:subClassOf obo:ECO_0000204 , obo:ECO_0000361 .

obo:OBI_0001573 a rdfs:Class ;
  rdfs:label "DNA sequence data" ;
  rdfs:comment "A sequence data item that is about the primary structure of DNA" ;
  rdfs:subClassOf obo:OBI_0000973 .

obo:ECO_0007350 a rdfs:Class ;
  rdfs:label "blocking polyclonal antibody evidence used in automatic assertion" ;
  rdfs:comment "A type of blocking polyclonal antibody evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001015 , obo:ECO_0007348 .

obo:ECO_0006196 a rdfs:Class ;
  rdfs:label "nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence used in manual assertion" ;
  rdfs:comment "A type of nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006165 , obo:ECO_0006175 .

obo:ECO_0007602 a rdfs:Class ;
  rdfs:label "compement fixation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of compement fixation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007031 , obo:ECO_0007348 .

obo:OBI_0000923 a rdfs:Class ;
  rdfs:label "surface plasmon resonance binding assay" ;
  rdfs:comment "A binding assay that uses the detection of electromagnetic waves in a surface to detect material entities adsorbed to the surface, which change the local optical index of refraction." ;
  rdfs:subClassOf obo:OBI_0001146 .

obo:ECO_0007194 a rdfs:Class ;
  rdfs:label "structural similarity evidence used in automatic assertion" ;
  rdfs:comment "A type of structural similarity evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000027 , obo:ECO_0007824 .

obo:ECO_0000214 a rdfs:Class ;
  rdfs:label "biological aspect of descendant evidence" ;
  rdfs:comment "A type of phylogenetic evidence whereby an aspect of an ancestral gene is inferred through the characterization of an aspect of a descendant gene." ;
  rdfs:subClassOf obo:ECO_0000080 .

obo:ECO_0001054 a rdfs:Class ;
  rdfs:label "immunodepletion evidence" ;
  rdfs:comment "A type of immunological assay evidence resulting from the use of antibodies to remove specific proteins from a sample." ;
  rdfs:subClassOf obo:ECO_0000040 .

obo:ECO_0007709 a rdfs:Class ;
  rdfs:label "transport assay evidence used in manual assertion" ;
  rdfs:comment "A type of transport assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000134 , obo:ECO_0000314 .

obo:ECO_0007686 a rdfs:Class ;
  rdfs:label "voltage clamp recording evidence used in manual assertion" ;
  rdfs:comment "A type of voltage clamp recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005576 , obo:ECO_0006006 .

obo:ECO_0008012 a rdfs:Class ;
  rdfs:label "regular expression method evidence" ;
  rdfs:comment "A type of string-matching method evidence in which patterns consisting of characters and/or symbols in particular combinations and/or order are used to identify matches in a larger string or body of text." ;
  rdfs:subClassOf obo:ECO_0008011 .

obo:GO_0032891 a rdfs:Class ;
  rdfs:label "negative regulation of organic acid transport" ;
  rdfs:subClassOf obo:GO_0032890 , obo:GO_0051051 , obo:GO_0065007 .

obo:GO_0003823 a rdfs:Class ;
  rdfs:label "antigen binding" ;
  rdfs:subClassOf obo:GO_0005488 .

obo:ECO_0001565 a rdfs:Class ;
  rdfs:label "cell-based assay evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from the study of cells." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:GO_0098630 a rdfs:Class ;
  rdfs:label "aggregation of unicellular organisms" ;
  rdfs:subClassOf obo:GO_0098743 .

obo:ECO_0007581 a rdfs:Class ;
  rdfs:label "suppressor/enhancer interaction phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of suppressor/enhancer interaction phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000052 , obo:ECO_0007326 .

obo:GO_0043393 a rdfs:Class ;
  rdfs:label "regulation of protein binding" ;
  rdfs:subClassOf obo:GO_0051098 , obo:GO_0065007 .

obo:GO_0055085 a rdfs:Class ;
  rdfs:label "transmembrane transport" ;
  rdfs:subClassOf obo:GO_0006810 , obo:GO_0009987 .

obo:GO_0042611 a rdfs:Class ;
  rdfs:label "MHC protein complex" ;
  rdfs:comment "A transmembrane protein complex composed of an MHC alpha chain and, in most cases, either an MHC class II beta chain or an invariant beta2-microglobin chain, and with or without a bound peptide, lipid, or polysaccharide antigen." ;
  rdfs:subClassOf obo:GO_0032991 .

obo:ECO_0007537 a rdfs:Class ;
  rdfs:label "patch-clamp recording evidence used in automatic assertion" ;
  rdfs:comment "A type of patch-clamp recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006012 , obo:ECO_0007535 .

obo:ECO_0005535 a rdfs:Class ;
  rdfs:label "machine learning prediction of motif instance evidence" ;
  rdfs:comment "A type of match to sequence model evidence which captures the application of a broad suite of supervised and unsupervised machine learning algorithms trained on known motif instances and applied to the prediction of novel putative instances of a motif in a set of biological sequences." ;
  rdfs:subClassOf obo:ECO_0000202 .

obo:OBI_0400153 a rdfs:Class ;
  rdfs:label "droplet sorter" ;
  rdfs:comment "A droplet sorter is part_of a flow cytometer sorter that converts the carrier fluid stream into individual droplets, and these droplets are directed into separate locations for recovery (enriching the original sample for particles of interest based on qualities determined by gating) or disposal." ;
  rdfs:subClassOf obo:OBI_0000932 .

obo:ECO_0007216 a rdfs:Class ;
  rdfs:label "protein separation followed by direct sequencing evidence used in automatic assertion" ;
  rdfs:comment "A type of protein separation followed by direct sequencing evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000158 , obo:ECO_0007787 .

obo:GO_0033554 a rdfs:Class ;
  rdfs:label "cellular response to stress" ;
  rdfs:subClassOf obo:GO_0006950 , obo:GO_0051716 .

obo:ECO_0001841 a rdfs:Class ;
  rdfs:label "colorimetric in situ hybridization evidence used in manual assertion" ;
  rdfs:comment "A type of colorimetric in situ hybridization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001838 , obo:ECO_0001839 .

obo:ECO_0005551 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence by experimental evidence" ;
  rdfs:comment "A type of biological system reconstruction evidence that uses experimental evidence as support." ;
  rdfs:subClassOf obo:ECO_0000088 .

obo:GO_1901564 a rdfs:Class ;
  rdfs:label "organonitrogen compound metabolic process" ;
  rdfs:subClassOf obo:GO_0006807 , obo:GO_0071704 .

obo:GO_0045727 a rdfs:Class ;
  rdfs:label "positive regulation of translation" ;
  rdfs:subClassOf obo:GO_0006417 , obo:GO_0010557 , obo:GO_0010628 , obo:GO_0031328 , obo:GO_0032270 , obo:GO_0034250 , obo:GO_0065007 .

obo:ECO_0006177 a rdfs:Class ;
  rdfs:label "circular dichroism evidence" ;
  rdfs:comment "A type of spectrometry evidence derived from irradiating a sample with light (either visible, infrared or ultraviolet wavelengths) and observing the difference of absorption of left-handed and right-handed light by the sample by comparing the extent of circular polarization in the incident and the emitted light." ;
  rdfs:subClassOf obo:ECO_0005031 , obo:ECO_0005504 .

obo:GO_1903828 a rdfs:Class ;
  rdfs:label "negative regulation of protein localization" ;
  rdfs:subClassOf obo:GO_0032880 , obo:GO_0048519 , obo:GO_0065007 .

obo:ECO_0007375 a rdfs:Class ;
  rdfs:label "cytochemistry evidence used in automatic assertion" ;
  rdfs:comment "A type of cytochemistry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001035 , obo:ECO_0007376 .

obo:GO_0043270 a rdfs:Class ;
  rdfs:label "positive regulation of ion transport" ;
  rdfs:subClassOf obo:GO_0043269 , obo:GO_0051050 , obo:GO_0065007 .

obo:ECO_0005625 a rdfs:Class ;
  rdfs:label "consensus search evidence used in automatic assertion" ;
  rdfs:comment "A type of consensus search evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000258 , obo:ECO_0005532 .

obo:ECO_0007107 a rdfs:Class ;
  rdfs:label "yellow fluorescent protein fusion protein localization evidence used in manual assertion" ;
  rdfs:comment "A type of yellow fluorescent protein fusion protein localization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000128 , obo:ECO_0000314 .

obo:OBI_0001681 a rdfs:Class ;
  rdfs:label "yeast one-hybrid assay" ;
  rdfs:comment "An assay that determines protein DNA interactions using a single fusion protein in which the activating domain is linked directly to the binding domain." ;
  rdfs:subClassOf obo:OBI_0001146 .

obo:ECO_0007758 a rdfs:Class ;
  rdfs:label "curator inference from journal publication used in manual assertion" ;
  rdfs:comment "A type of curator inference from journal publication that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007645 , obo:ECO_0007752 .

obo:GO_0051093 a rdfs:Class ;
  rdfs:label "negative regulation of developmental process" ;
  rdfs:subClassOf obo:GO_0048519 , obo:GO_0050793 , obo:GO_0065007 .

obo:COB_0000080 a rdfs:Class ;
  rdfs:label "complex of molecular entities" ;
  rdfs:comment "A complex of two or more molecular entities that are not covalently bound." ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:OBI_0500000 a rdfs:Class ;
  rdfs:label "study design"@en ;
  rdfs:comment "A plan specification comprised of protocols (which may specify how and what kinds of data will be gathered) that are executed as part of an investigation and is realized during a study design execution." ;
  rdfs:subClassOf obo:IAO_0000104 .

obo:ECO_0008054 a rdfs:Class ;
  rdfs:label "antibody C7709A2-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody C7709A2 to determine that HLA protein complex with A24 serotype presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0006410 a rdfs:Class ;
  rdfs:label "combinatorial intrinsic disorder prediction and experimental evidence used in manual assertion" ;
  rdfs:comment "A type of combinatorial intrinsic disorder prediction and experimental evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006408 , obo:ECO_0007744 .

obo:ECO_0001016 a rdfs:Class ;
  rdfs:label "blood test evidence" ;
  rdfs:comment "A type of direct assay evidence where a blood sample is extracted from an organism to analyze different blood components." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007306 a rdfs:Class ;
  rdfs:label "qualitative western immunoblotting evidence used in automatic assertion" ;
  rdfs:comment "A type of qualitative western immunoblotting evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000112 , obo:ECO_0007309 , obo:ECO_0007770 , obo:ECO_0007796 .

obo:ECO_0007494 a rdfs:Class ;
  rdfs:label "immunoperoxidase immunolocalization evidence used in automatic assertion" ;
  rdfs:comment "A type of immunoperoxidase immunolocalization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005594 , obo:ECO_0007486 .

obo:ECO_0005506 a rdfs:Class ;
  rdfs:label "missense mutation phenotypic evidence" ;
  rdfs:comment "A type of point mutation phenotypic evidence resulting from a change in a base (or bases) causing the substitution of one amino acid for another." ;
  rdfs:subClassOf obo:ECO_0001113 .

obo:ECO_0007742 a rdfs:Class ;
  rdfs:label "3C evidence used in manual assertion" ;
  rdfs:comment "A type of 3C evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000233 , obo:ECO_0000269 .

obo:GO_0032502 a rdfs:Class ;
  rdfs:label "developmental process" ;
  rdfs:subClassOf obo:GO_0008150 .

obo:ECO_0007082 a rdfs:Class ;
  rdfs:label "colony size phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of colony size phenotypic evidence that is used in an manual assertion." ;
  rdfs:subClassOf obo:ECO_0007078 , obo:ECO_0007081 .

obo:OBI_0001121 a rdfs:Class ;
  rdfs:label "gel electrophoresis system" ;
  rdfs:comment "An electrophoresis system in which an electric field is applied to a gel matrix" ;
  rdfs:subClassOf obo:OBI_0001053 .

obo:ECO_0006215 a rdfs:Class ;
  rdfs:label "small-angle neutron scattering evidence used in automatic assertion" ;
  rdfs:comment "A type of small-angle neutron scattering evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006184 , obo:ECO_0006213 .

obo:ECO_0007259 a rdfs:Class ;
  rdfs:label "differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) used in automatic assertion" ;
  rdfs:comment "A type of differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000359 , obo:ECO_0007303 .

obo:ECO_0007624 a rdfs:Class ;
  rdfs:label "phenomic profiling assay evidence used in automatic assertion" ;
  rdfs:comment "A type of phenomic profiling assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007075 , obo:ECO_0007635 .

obo:ECO_0000269 a rdfs:Class ;
  rdfs:label "experimental evidence used in manual assertion" ;
  rdfs:comment "A type of experimental evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/exp-inferred-experiment> ;
  rdfs:subClassOf obo:ECO_0000006 , obo:ECO_0000352 .

obo:ECO_0007322 a rdfs:Class ;
  rdfs:label "curator inference used in automatic assertion" ;
  rdfs:comment "A type of curator inference that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000205 , obo:ECO_0007832 .

obo:ECO_0006023 a rdfs:Class ;
  rdfs:label "microtiter plate biofilm assay evidence used in manual assertion" ;
  rdfs:comment "A type of microtiter plate biofilm assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006021 , obo:ECO_0006022 .

obo:ECO_0006267 a rdfs:Class ;
  rdfs:label "pH-induced protein unfolding evidence" ;
  rdfs:comment "A type of protein unfolding evidence where the unfolding of the protein is triggered by changes in pH." ;
  rdfs:subClassOf obo:ECO_0006265 .

obo:ECO_0006151 a rdfs:Class ;
  rdfs:label "documented statement evidence" ;
  rdfs:comment "A type of evidence in which information is recorded in some documentation system, for example, but not limited to a publication, survey, or medical record." ;
  rdfs:subClassOf obo:ECO_0000000 .

obo:GO_0042391 a rdfs:Class ;
  rdfs:label "regulation of membrane potential" ;
  rdfs:subClassOf obo:GO_0065008 .

obo:ECO_0006174 a rdfs:Class ;
  rdfs:label "mixed support of intron positions by RNA-sequencing alignment evidence used in manual assertion" ;
  rdfs:comment "A type of mixed support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion" ;
  rdfs:subClassOf obo:ECO_0000346 , obo:ECO_0006172 .

obo:PATO_0000169 a rdfs:Class ;
  rdfs:label "viability" ;
  rdfs:comment "An organismal quality inhering in a bearer or a population by virtue of the bearer's disposition to survive and develop normally or the number of surviving individuals in a given population." ;
  rdfs:subClassOf obo:PATO_0001995 .

obo:ECO_0000253 a rdfs:Class ;
  rdfs:label "genetic similarity evidence used in manual assertion" ;
  rdfs:comment "A type of genetic similarity evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000051 , obo:ECO_0000252 .

obo:GO_0042605 a rdfs:Class ;
  rdfs:label "peptide antigen binding" ;
  rdfs:subClassOf obo:GO_0003823 , obo:GO_0042277 .

obo:ECO_0008057 a rdfs:Class ;
  rdfs:label "antibody DT9-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody DT9 to determine that the HLA-C protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0001834 a rdfs:Class ;
  rdfs:label "infection assay evidence used in automatic assertion" ;
  rdfs:comment "A type of infection assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001831 , obo:ECO_0001833 .

obo:ECO_0001131 a rdfs:Class ;
  rdfs:label "transgenic organism evidence" ;
  rdfs:comment "A type of genetic transformation evidence resulting from an organism that has had its expressed phenotype altered by modification." ;
  rdfs:subClassOf obo:ECO_0005027 .

obo:ECO_0000320 a rdfs:Class ;
  rdfs:label "phylogenetic determination of loss of key residues evidence used in manual assertion" ;
  rdfs:comment "A type of phylogenetic determination of loss of key residues evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/ikr-inferred-key-residues> ;
  rdfs:subClassOf obo:ECO_0000216 , obo:ECO_0000252 .

obo:ECO_0007724 a rdfs:Class ;
  rdfs:label "localization evidence used in manual assertion" ;
  rdfs:comment "A type of localization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001533 .

obo:ECO_0007655 a rdfs:Class ;
  rdfs:label "manually integrated combinatorial experimental evidence" ;
  rdfs:comment "A type of manually integrated combinatorial evidence in which two or more distinct types of experimental evidence are integrated manually." ;
  rdfs:subClassOf obo:ECO_0007654 , obo:ECO_0007674 .

obo:OBI_0000699 a rdfs:Class ;
  rdfs:label "survival assessment assay" ;
  rdfs:comment "An assay that measures the occurrence of death events in one or more organisms over time" ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0001222 a rdfs:Class ;
  rdfs:label "in vivo ubiquitination assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo ubiquitination assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001089 .

obo:ECO_0007778 a rdfs:Class ;
  rdfs:label "cell growth assay evidence used in automatic assertion" ;
  rdfs:comment "A type of cell growth assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001563 , obo:ECO_0007773 .

obo:ECO_0008192 a rdfs:Class ;
  rdfs:label "avidity evidence used in manual assertion" ;
  rdfs:comment "A type of avidity evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000353 , obo:ECO_0008185 .

obo:ECO_0001032 a rdfs:Class ;
  rdfs:label "constitutively active mutant protein evidence" ;
  rdfs:comment "A type of mutant phenotypic evidence resulting from a mutation causing a change to a protein sequence that produces a constitutively active mutant protein (CAM), that remains active in the absence of upstream signals." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0001552 a rdfs:Class ;
  rdfs:label "deubiquitination assay evidence" ;
  rdfs:comment "A type of direct assay evidence where the removal of ubiquitin groups from a protein is detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0001247 a rdfs:Class ;
  rdfs:label "point mutation phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of point mutation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0001113 .

obo:OBI_0002178 a rdfs:Class ;
  rdfs:label "whole-cell patch clamp assay" ;
  rdfs:comment "A patch-clamp assay where the electrode is left in place on the cell, as in cell-attached recordings, but the membrane patch has been perforated, providing access from the interior of the pipette to the intracellular space of the cell. Measurements made with this technique involve recording currents through multiple channels simultaneously, over the membrane of the entire cell." ;
  rdfs:subClassOf obo:OBI_0002177 .

obo:ECO_0007125 a rdfs:Class ;
  rdfs:label "Illumina sequencing evidence used in manual assertion" ;
  rdfs:comment "A type of Illumina sequencing evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000222 , obo:ECO_0000269 .

obo:ECO_0006327 a rdfs:Class ;
  rdfs:label "dynamic fluorescence quenching evidence used in manual assertion" ;
  rdfs:comment "A type of dynamic fluorescence quenching evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000353 , obo:ECO_0001249 , obo:ECO_0006273 .

obo:ECO_0006256 a rdfs:Class ;
  rdfs:label "protein fragment functional complementation evidence" ;
  rdfs:comment "A type of physical interaction evidence that is based on the detection of a protein-protein interaction between a bait and prey protein by reconstitution of a reporter protein serving as a detectable entity (usually an enzyme or a fluorescent protein), the two halves of which are each attached separately to the bait and prey." ;
  rdfs:subClassOf obo:ECO_0000021 .

obo:ECO_0008065 a rdfs:Class ;
  rdfs:label "antibody LB3.1-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody LB3.13 to determine that the HLA-DR protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0000055 a rdfs:Class ;
  rdfs:label "obsolete array experiment evidence" ;
  rdfs:comment "This general 'array experiment' term should not be used - replace with the specific type of array (e.g. ECO:0000097, ECO:0000062, ECO:0000104, etc.)" .

obo:ECO_0006135 a rdfs:Class ;
  rdfs:label "quantum mechanics/molecular mechanics simulation evidence" ;
  rdfs:comment "A type of computational evidence where the active site of the molecular system is described with highly accurate quantum theory, while the contribution of the rest of the system is described with molecular mechanical force field." ;
  rdfs:subClassOf obo:ECO_0007672 .

obo:ECO_0006160 a rdfs:Class ;
  rdfs:label "self-reported patient statement evidence used in automatic assertion" ;
  rdfs:comment "A type of self-reported patient statement evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006154 , obo:ECO_0006158 .

obo:ECO_0006375 a rdfs:Class ;
  rdfs:label "qualitative macroscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of qualitative macroscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006342 , obo:ECO_0007430 .

obo:ECO_0007403 a rdfs:Class ;
  rdfs:label "in vitro phosphatase assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro phosphatase assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001067 , obo:ECO_0007772 .

obo:ECO_0006038 a rdfs:Class ;
  rdfs:label "genetically encoded fluorescent electrophysiology assay evidence" ;
  rdfs:comment "A type of fluorescent sensor evidence and electrophysiology assay evidence where the electrical properties of cells or tissues are studied using fluorescent sensors." ;
  rdfs:subClassOf obo:ECO_0000164 , obo:ECO_0006034 .

obo:ECO_0006350 a rdfs:Class ;
  rdfs:label "bio-layer interferometry evidence" ;
  rdfs:comment "A type of molecule detection assay evidence in which the detection of the interaction between molecules is done without the use of labels and is instead based on the phase shift-wavelength correlation created between interference patterns off of two unique surfaces on the tip of a biosensor, where the ligand is immobilized onto a bio compatible biosensor while the analyte is in solution." ;
  rdfs:subClassOf obo:ECO_0005516 .

obo:GO_0031331 a rdfs:Class ;
  rdfs:label "positive regulation of cellular catabolic process" ;
  rdfs:subClassOf obo:GO_0009896 , obo:GO_0031325 , obo:GO_0031329 , obo:GO_0065007 .

obo:ECO_0006317 a rdfs:Class ;
  rdfs:label "temperature-induced protein unfolding evidence used in manual assertion" ;
  rdfs:comment "A type of temperature-induced protein unfolding evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006268 , obo:ECO_0006311 .

obo:ECO_0005610 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence based on homology evidence used in manual assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence based on homology evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005549 , obo:ECO_0007746 .

obo:ECO_0007552 a rdfs:Class ;
  rdfs:label "cell fractionation evidence used in automatic assertion" ;
  rdfs:comment "A type of cell fractionation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000004 , obo:ECO_0007534 .

obo:ECO_0007767 a rdfs:Class ;
  rdfs:label "protein kinase assay evidence used in automatic assertion" ;
  rdfs:comment "A type of protein kinase assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001558 , obo:ECO_0007468 .

obo:ECO_0008024 a rdfs:Class ;
  rdfs:label "neural network method evidence used in manual assertion" ;
  rdfs:comment "A type of neural network method evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008003 , obo:ECO_0008013 .

obo:GO_1903506 a rdfs:Class ;
  rdfs:label "regulation of nucleic acid-templated transcription" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_2001141 .

obo:GO_1900131 a rdfs:Class ;
  rdfs:label "negative regulation of lipid binding" ;
  rdfs:subClassOf obo:GO_0051100 , obo:GO_0065007 , obo:GO_1900130 .

obo:ECO_0001576 a rdfs:Class ;
  rdfs:label "membrane fusion assay evidence used in manual assertion" ;
  rdfs:comment "A type of membrane fusion assay evidence that is used in manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001574 .

obo:ECO_0001049 a rdfs:Class ;
  rdfs:label "gel-filtration evidence" ;
  rdfs:comment "A type of chromatography evidence resulting from the exclusion of proteins based on molecular size by filtration through a porous matrix (swollen gel), from which larger molecules are excluded." ;
  rdfs:subClassOf obo:ECO_0000325 .

obo:CL_0000842 a rdfs:Class ;
  rdfs:label "mononuclear cell" ;
  rdfs:comment "A leukocyte with a single non-segmented nucleus in the mature form." ;
  rdfs:subClassOf obo:CL_0000000 .

obo:ECO_0001258 a rdfs:Class ;
  rdfs:label "spectrophotometry evidence used in manual assertion" ;
  rdfs:comment "A type of spectrophotometry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001126 .

obo:GO_0006974 a rdfs:Class ;
  rdfs:label "cellular response to DNA damage stimulus" ;
  rdfs:comment "Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism." ;
  rdfs:subClassOf obo:GO_0033554 .

obo:ECO_0008155 a rdfs:Class ;
  rdfs:label "antibody SPVL3-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody SPVL3-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008073 , obo:ECO_0008087 .

obo:OBI_0001033 a rdfs:Class ;
  rdfs:label "perturbation device" ;
  rdfs:comment "A perturbation device is a device which is designed to perform a perturb function" ;
  rdfs:subClassOf obo:OBI_0000968 .

obo:ECO_0007775 a rdfs:Class ;
  rdfs:label "cell proliferation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of cell proliferation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005007 , obo:ECO_0007773 .

obo:ECO_0007713 a rdfs:Class ;
  rdfs:label "acetylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of acetylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001546 .

obo:GO_0098781 a rdfs:Class ;
  rdfs:label "ncRNA transcription" ;
  rdfs:subClassOf obo:GO_0006351 .

obo:ECO_0001117 a rdfs:Class ;
  rdfs:label "protein microarray evidence" ;
  rdfs:comment "A type of protein detection assay evidence resulting from proteins immobilized to prepare protein chips, or microwells, that can be utilized to determine presence of proteins, monitor differential expression profiles, and/or study protein interactions." ;
  rdfs:subClassOf obo:ECO_0005517 .

obo:OBI_0000931 a rdfs:Class ;
  rdfs:label "study intervention" ;
  rdfs:comment "the part of the execution of an intervention design study which is varied between two or more subjects in the study" ;
  rdfs:subClassOf obo:OBI_0000011 .

obo:ECO_0006258 a rdfs:Class ;
  rdfs:label "GAL4-VP16 functional complementation evidence" ;
  rdfs:comment "A type of protein fragment functional complementaion evidence where the interaction between bait and prey assemble a transcriptional activation complex consisting of the GAL4 DNA-binding domain and the transactivation domain from the herpes simplex virus protein VP16." ;
  rdfs:subClassOf obo:ECO_0006256 .

obo:ECO_0000080 a rdfs:Class ;
  rdfs:label "phylogenetic evidence" ;
  rdfs:comment "A type of similarity that indicates common ancestry." ;
  rdfs:subClassOf obo:ECO_0000041 .

obo:ECO_0001193 a rdfs:Class ;
  rdfs:label "in vitro defarnesylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro defarnesylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001061 .

obo:GO_0098900 a rdfs:Class ;
  rdfs:label "regulation of action potential" ;
  rdfs:subClassOf obo:GO_0042391 , obo:GO_0050789 .

obo:GO_1903034 a rdfs:Class ;
  rdfs:label "regulation of response to wounding" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_0080134 .

obo:ECO_0007258 a rdfs:Class ;
  rdfs:label "differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) used in automatic assertion" ;
  rdfs:comment "A type of differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000358 , obo:ECO_0007303 .

obo:ECO_0007118 a rdfs:Class ;
  rdfs:label "mutant visible phenotype evidence used in manual assertion" ;
  rdfs:comment "A type of mutant visible phenotype evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000176 , obo:ECO_0000315 .

obo:ECO_0001598 a rdfs:Class ;
  rdfs:label "multilocus sequence typing evidence" ;
  rdfs:comment "A type of nucleotide sequencing assay evidence in which DNA sequence variations within a specific set of genes (typically housekeeping genes) are used to characterizes strains by their unique allelic profiles." ;
  rdfs:subClassOf obo:ECO_0000219 .

obo:ECO_0008046 a rdfs:Class ;
  rdfs:label "antibody 4B7/8-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody 4B7/8 to determine that the pig MHC class I protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0005654 a rdfs:Class ;
  rdfs:label "phylogenetic footprinting evidence used in manual assertion" ;
  rdfs:comment "A type of phylogenetic footprinting evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005533 , obo:ECO_0005558 .

obo:ECO_0007484 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence based on inference from background scientific knowledge used in automatic assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005548 , obo:ECO_0007827 .

obo:ECO_0007479 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence by experimental evidence from single species used in automatic assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence by experimental evidence from single species that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005553 , obo:ECO_0007828 .

obo:OBI_0002119 a rdfs:Class ;
  rdfs:label "microscopy assay" ;
  rdfs:comment "An imaging assay that utilizes a microscope to magnify features of the visualized material of interest that are not visible to naked eye." ;
  rdfs:subClassOf obo:OBI_0000185 .

obo:ECO_0007414 a rdfs:Class ;
  rdfs:label "in vivo demethylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo demethylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001078 , obo:ECO_0007795 .

obo:GO_1901575 a rdfs:Class ;
  rdfs:label "organic substance catabolic process" ;
  rdfs:subClassOf obo:GO_0009056 , obo:GO_0071704 .

obo:ECO_0006218 a rdfs:Class ;
  rdfs:label "combinatorial experimental and author inference evidence contained in single publication used in manual assertion" ;
  rdfs:comment "A type of combinatorial experimental and author inference evidence contained in single publication that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006186 , obo:ECO_0007013 .

obo:OBI_0302887 a rdfs:Class ;
  rdfs:label "staining"@en ;
  rdfs:comment "Staining is a process which results in the addition a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound."@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:GO_0071495 a rdfs:Class ;
  rdfs:label "cellular response to endogenous stimulus" ;
  rdfs:subClassOf obo:GO_0009719 .

obo:ECO_0001204 a rdfs:Class ;
  rdfs:label "in vitro transcription assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro transcription assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001071 .

obo:ECO_0007623 a rdfs:Class ;
  rdfs:label "bacterial 2-hybrid assay evidence used in automatic assertion" ;
  rdfs:comment "A type of bacterial 2-hybrid assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007073 , obo:ECO_0007432 .

obo:ECO_0007169 a rdfs:Class ;
  rdfs:label "in-gel protein kinase assay evidence used in manual assertion" ;
  rdfs:comment "A type of in-gel protein kinase assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005562 , obo:ECO_0005801 .

obo:ECO_0007439 a rdfs:Class ;
  rdfs:label "multiplex bead-based immunoassay evidence used in automatic assertion" ;
  rdfs:comment "A type of multiplex bead-based immunoassay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001102 , obo:ECO_0007819 .

obo:ECO_0000073 a rdfs:Class ;
  rdfs:label "obsolete experimental genomic evidence" ;
  rdfs:comment "OBSOLETE. A type of experimental evidence that is based on the characterization of an attribute of the genome underlying a gene product." .

obo:ECO_0007042 a rdfs:Class ;
  rdfs:label "epifluorescence microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of epifluorescence microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006323 , obo:ECO_0007041 .

obo:ECO_0007022 a rdfs:Class ;
  rdfs:label "slide agglutination test evidence used in manual assertion" ;
  rdfs:comment "A type of slide agglutination test evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007020 , obo:ECO_0007021 .

obo:ECO_0005631 a rdfs:Class ;
  rdfs:label "DNAse footprinting evidence used in manual assertion" ;
  rdfs:comment "A type of DNAse footprinting evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001810 , obo:ECO_0006083 .

obo:ECO_0000102 a rdfs:Class ;
  rdfs:label "co-fractionation evidence" ;
  rdfs:comment "A type of fractionation evidence resulting from a protein fractionated with other compounds, factors, or macromolecules." ;
  rdfs:subClassOf obo:ECO_0000100 .

obo:OBI_0000384 a rdfs:Class ;
  rdfs:label "electricity supply function"@en ;
  rdfs:comment "An electricity supply function is an energy supply function to transfer electricity from one source to another, typically a consumer of the electricity or as a stimulus during a neuroscience experiment."@en ;
  rdfs:subClassOf obo:OBI_0000391 .

obo:ECO_0001225 a rdfs:Class ;
  rdfs:label "knockout evidence used in manual assertion" ;
  rdfs:comment "A type of knockout evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001091 , obo:ECO_0006049 .

obo:ECO_0007269 a rdfs:Class ;
  rdfs:label "frameshift mutation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of frameshift mutation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005512 , obo:ECO_0007325 .

obo:ECO_0006247 a rdfs:Class ;
  rdfs:label "analytical ultracentrifugation evidence" ;
  rdfs:comment "A type of molecule detection assay evidence where the hydrodynamic behaviour of macromolecules is used to study their size, shape and interactions, by spinning the sample solution at high centrifugal field (up to above 100000 rpm and 1000000 g), and monitoring the evolution of sample concentration profile versus the axis of rotation." ;
  rdfs:subClassOf obo:ECO_0005516 .

obo:GO_0040007 a rdfs:Class ;
  rdfs:label "growth" ;
  rdfs:subClassOf obo:GO_0008150 .

obo:ECO_0005642 a rdfs:Class ;
  rdfs:label "heteronuclear single quantum coherence spectroscopy evidence used in manual assertion" ;
  rdfs:comment "A type of heteronuclear single quantum coherence spectroscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001813 , obo:ECO_0006165 .

obo:ECO_0006140 a rdfs:Class ;
  rdfs:label "molecular mechanics simulation evidence used in manual assertion" ;
  rdfs:comment "A type of molecular mechanics simulation evidence that is used in manual assertion." ;
  rdfs:subClassOf obo:ECO_0006138 .

obo:GO_0051047 a rdfs:Class ;
  rdfs:label "positive regulation of secretion" ;
  rdfs:subClassOf obo:GO_0051046 , obo:GO_0051050 , obo:GO_0065007 .

obo:ECO_0006349 a rdfs:Class ;
  rdfs:label "iodine-125-labeled ligand binding evidence" ;
  rdfs:comment "A type of radioligand binding assay evidence in which iodine-125 label is used to measure receptor-ligand interactions, such as in determining selectivity for a particular ligand." ;
  rdfs:subClassOf obo:ECO_0007008 .

obo:ECO_0001215 a rdfs:Class ;
  rdfs:label "in vivo methylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo methylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001082 .

obo:OBI_0000318 a rdfs:Class ;
  rdfs:label "eluate"@en ;
  rdfs:comment "a eluate is a material entity which results from an elution, e.g. from a chromatography column. it has as part a material entity with role mobile phase"@en ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:ECO_0008113 a rdfs:Class ;
  rdfs:label "antibody B8-24-3-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody B8-24-3-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008052 , obo:ECO_0008087 .

obo:ECO_0007299 a rdfs:Class ;
  rdfs:label "Affymetrix GeneChip evidence used in automatic assertion" ;
  rdfs:comment "A type of Affymetrix GeneChip evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000101 , obo:ECO_0007300 .

obo:ECO_0007391 a rdfs:Class ;
  rdfs:label "immunodepletion evidence used in automatic assertion" ;
  rdfs:comment "A type of immunodepletion evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001054 , obo:ECO_0007348 .

obo:ECO_0007458 a rdfs:Class ;
  rdfs:label "spectrophotometry evidence used in automatic assertion" ;
  rdfs:comment "A type of spectrophotometry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001126 , obo:ECO_0007821 .

obo:ECO_0001141 a rdfs:Class ;
  rdfs:label "[3H]-thymidine incorporation assay evidence used in manual assertion" ;
  rdfs:comment "A type of [3H]-thymidine incorporation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001003 , obo:ECO_0007697 .

obo:ECO_0006278 a rdfs:Class ;
  rdfs:label "fluorescence polarization evidence used in automatic assertion" ;
  rdfs:comment "A type of fluorescence polarization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006248 , obo:ECO_0007332 .

obo:ECO_0006207 a rdfs:Class ;
  rdfs:label "near-UV circular dichroism evidence used in automatic assertion" ;
  rdfs:comment "A type of near-UV circular dichroism evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006180 , obo:ECO_0006201 .

obo:GO_0048640 a rdfs:Class ;
  rdfs:label "negative regulation of developmental growth" ;
  rdfs:subClassOf obo:GO_0045926 , obo:GO_0048638 , obo:GO_0051093 , obo:GO_0065007 .

obo:ECO_0001092 a rdfs:Class ;
  rdfs:label "lipid binding assay evidence" ;
  rdfs:comment "A type of physical interaction evidence resulting from the qualitative and quantitative analysis of the affinity with which a protein binds to a lipid." ;
  rdfs:subClassOf obo:ECO_0000021 .

obo:ECO_0007339 a rdfs:Class ;
  rdfs:label "[3H]arachidonic acid release assay evidence used in automatic assertion" ;
  rdfs:comment "A type of [3H]arachidonic acid release assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001002 , obo:ECO_0007773 .

obo:GO_0048869 a rdfs:Class ;
  rdfs:label "cellular developmental process" ;
  rdfs:subClassOf obo:GO_0009987 , obo:GO_0032502 .

obo:ECO_0007425 a rdfs:Class ;
  rdfs:label "in vivo ubiquitination assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo ubiquitination assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001089 , obo:ECO_0007801 .

obo:GO_0090237 a rdfs:Class ;
  rdfs:label "regulation of arachidonic acid secretion" ;
  rdfs:subClassOf obo:GO_0032303 , obo:GO_0065007 .

obo:ECO_0000258 a rdfs:Class ;
  rdfs:label "motif similarity evidence used in automatic assertion" ;
  rdfs:comment "A type of motif similarity evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000028 , obo:ECO_0000256 .

obo:ECO_0007114 a rdfs:Class ;
  rdfs:label "heterologous system uptake evidence used in manual assertion" ;
  rdfs:comment "A type of heterologous system uptake evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000162 , obo:ECO_0000314 .

obo:ECO_0007642 a rdfs:Class ;
  rdfs:label "curator inference from Oxford Dictionary" ;
  rdfs:comment "A type of curator inference from dictionary in which the reference is to an entry in the Oxford Dictionaries." ;
  rdfs:subClassOf obo:ECO_0007641 .

obo:GO_0030041 a rdfs:Class ;
  rdfs:label "actin filament polymerization" ;
  rdfs:comment "Assembly of actin filaments by the addition of actin monomers to a filament." ;
  rdfs:subClassOf obo:GO_0008150 .

obo:BFO_0000006 a rdfs:Class ;
  rdfs:label "spatial region"@en ;
  rdfs:subClassOf obo:BFO_0000141 .

obo:GO_0048523 a rdfs:Class ;
  rdfs:label "negative regulation of cellular process" ;
  rdfs:subClassOf obo:GO_0048519 , obo:GO_0050794 , obo:GO_0065007 .

obo:ECO_0007505 a rdfs:Class ;
  rdfs:label "nucleic acid binding evidence used in automatic assertion" ;
  rdfs:comment "A type of nucleic acid binding evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000136 , obo:ECO_0007384 .

obo:ECO_0005529 a rdfs:Class ;
  rdfs:label "random mutagenesis phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence where a mutagenic product or process is used to alter the nucleotide sequence and thus induce genetic mutation.  Examples include, but are not limited to mutagenic compounds, irradiation, PCR w/ degenerate primers, etc." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0008102 a rdfs:Class ;
  rdfs:label "antibody 4B7/8-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody 4B7/8-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008046 , obo:ECO_0008088 .

obo:ECO_0001151 a rdfs:Class ;
  rdfs:label "blocking peptide evidence used in manual assertion" ;
  rdfs:comment "A type of blocking peptide evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001014 , obo:ECO_0006078 .

obo:ECO_0007609 a rdfs:Class ;
  rdfs:label "scanning electron microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of scanning electron microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007045 , obo:ECO_0007493 .

obo:ECO_0007445 a rdfs:Class ;
  rdfs:label "peptide affinity enrichment evidence used in automatic assertion" ;
  rdfs:comment "A type of peptide affinity enrichment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001110 , obo:ECO_0007384 .

obo:GO_1901328 a rdfs:Class ;
  rdfs:label "response to cytochalasin B" ;
  rdfs:subClassOf obo:GO_0010243 , obo:GO_0014070 , obo:GO_1901700 .

obo:GO_0010876 a rdfs:Class ;
  rdfs:label "lipid localization" ;
  rdfs:subClassOf obo:GO_0033036 .

obo:ECO_0001554 a rdfs:Class ;
  rdfs:label "methylation assay evidence" ;
  rdfs:comment "A type of direct assay evidence where methylated residues of a substarte (RNA/DNA or protein) are detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0006297 a rdfs:Class ;
  rdfs:label "GAL4-VP16 functional complementation evidence used in manual assertion" ;
  rdfs:comment "A type of GAL4-VP16 functional complementation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006258 , obo:ECO_0006293 .

obo:ECO_0007538 a rdfs:Class ;
  rdfs:label "whole-cell patch-clamp recording evidence used in automatic assertion" ;
  rdfs:comment "A type of whole-cell patch-clamp recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006014 , obo:ECO_0007537 .

obo:GO_0010557 a rdfs:Class ;
  rdfs:label "positive regulation of macromolecule biosynthetic process" ;
  rdfs:subClassOf obo:GO_0009891 , obo:GO_0010556 , obo:GO_0010604 , obo:GO_0065007 .

obo:ECO_0005562 a rdfs:Class ;
  rdfs:label "in-gel protein kinase assay evidence" ;
  rdfs:comment "A type of protein kinase assay evidence resulting from the evaluation of the phosphorylation activity of kinase on a gel-incorporated kinase substrate." ;
  rdfs:subClassOf obo:ECO_0001558 .

obo:ECO_0007570 a rdfs:Class ;
  rdfs:label "far-Western blotting evidence used in automatic assertion" ;
  rdfs:comment "A type of far-Western blotting evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000076 , obo:ECO_0007332 .

obo:UBERON_0000178 a rdfs:Class ;
  rdfs:label "blood" ;
  rdfs:comment "A fluid that is composed of blood plasma and erythrocytes." ;
  rdfs:subClassOf obo:UBERON_0006314 .

obo:ECO_0007386 a rdfs:Class ;
  rdfs:label "fluorescence in situ hybridization evidence used in automatic assertion" ;
  rdfs:comment "A type of fluorescence in situ hybridization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001047 , obo:ECO_0001837 , obo:ECO_0007809 .

obo:OBI_0666667 a rdfs:Class ;
  rdfs:label "nucleic acid extraction"@en ;
  rdfs:comment "a material separation to recover the nucleic acid fraction of an input material"@en ;
  rdfs:subClassOf obo:OBI_0302884 .

obo:GO_0060966 a rdfs:Class ;
  rdfs:label "regulation of gene silencing by RNA" ;
  rdfs:subClassOf obo:GO_0010468 , obo:GO_0065007 .

obo:ECO_0001160 a rdfs:Class ;
  rdfs:label "chemiluminescence-linked immunoassay evidence used in manual assertion" ;
  rdfs:comment "A type of chemiluminescence-linked immunoassay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001023 .

obo:ECO_0008028 a rdfs:Class ;
  rdfs:label "inference of intermediate molecular structure from structure of final molecule used in manual assertion" ;
  rdfs:comment "A type of inference of intermediate molecular structure from structure of final molecule that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000305 , obo:ECO_0008015 .

obo:ECO_0007631 a rdfs:Class ;
  rdfs:label "computational inference used in manual assertion" ;
  rdfs:comment "A type of evidence based on computational logical inference that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000362 , obo:ECO_0007668 , obo:ECO_0007751 .

obo:GO_0048639 a rdfs:Class ;
  rdfs:label "positive regulation of developmental growth" ;
  rdfs:subClassOf obo:GO_0045927 , obo:GO_0048638 , obo:GO_0051094 , obo:GO_0065007 .

obo:OBI_0001670 a rdfs:Class ;
  rdfs:label "RNA protection assay" ;
  rdfs:comment "An assay that determines the presence and estimates abundance of transcript species by first creating an homo or heteroduplex by adding a specific, complementary sequence to the sequence of interest and then exposing the mixture of ribonuclease, which will degrade only single stranded molecules. A detection step will reveal if the sample contained a sequence of interest." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007731 a rdfs:Class ;
  rdfs:label "spectrometry evidence used in manual assertion" ;
  rdfs:comment "A type of spectrometry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0005504 .

obo:ECO_0008043 a rdfs:Class ;
  rdfs:label "antibody 34-1-2S-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody 34-1-2S to determine that the mouse MHC class I protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0006034 a rdfs:Class ;
  rdfs:label "fluorescent sensor evidence" ;
  rdfs:comment "A type of fluorescence evidence that is based on direct, quantitative measurement of some cellular property using a fluorescent sensor." ;
  rdfs:subClassOf obo:ECO_0001115 .

obo:ECO_0001005 a rdfs:Class ;
  rdfs:label "7-aminoactinomycin staining evidence" ;
  rdfs:comment "A type of apoptotic assay evidence resulting from the use of 7-aminoactinomycin D (7-AAD) to distinguish viable, apoptotic, and dead cells, using the fact that permeability of the cell membrane, and fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells." ;
  rdfs:subClassOf obo:ECO_0005019 .

obo:OBI_0000047 a rdfs:Class ;
  rdfs:label "processed material"@en ;
  rdfs:comment "Is a material entity that is created or changed during material processing."@en ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:OBI_1110086 a rdfs:Class ;
  rdfs:label "restricting MHC role"@en ;
  rdfs:comment "The role played by an MHC molecule by binding a material entity playing the role of epitope when that epitope/MHC molecule pair are recognized (bound) by a TCR molecule on the surface of a cell playing the role of effector cell."@en ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:ECO_0001580 a rdfs:Class ;
  rdfs:label "liquid chromatography coupled with tandem mass spectrometry evidence" ;
  rdfs:comment "A type of mass spectrometry evidence where liquid chromatography is used to separate particles in solution, followed by fragmentation and measurement of the mass-to-charge ratio of the resulting particles to identify the amount and type of material entities present in a sample." ;
  rdfs:subClassOf obo:ECO_0001096 .

obo:ECO_0007398 a rdfs:Class ;
  rdfs:label "in vitro desumoylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro desumoylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001063 , obo:ECO_0007802 .

obo:ECO_0007002 a rdfs:Class ;
  rdfs:label "high throughput genetic interaction phenotypic evidence" ;
  rdfs:comment "A type of high throughput evidence resulting from the high throughput analysis of the effect that a given gene has on another gene or genes, and products." ;
  rdfs:subClassOf obo:ECO_0000011 , obo:ECO_0006055 .

obo:ECO_0007071 a rdfs:Class ;
  rdfs:label "flow cell biofilm assay evidence" ;
  rdfs:comment "A type of biofilm formation assay evidence derived from in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow." ;
  rdfs:subClassOf obo:ECO_0006020 .

obo:ECO_0008118 a rdfs:Class ;
  rdfs:label "antibody C7709A2-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody C7709A2-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008054 , obo:ECO_0008088 .

obo:ECO_0000178 a rdfs:Class ;
  rdfs:label "in vivo assay evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from a sample of microorganisms or living tissue grown in a medium." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:OBI_0003121 a rdfs:Class ;
  rdfs:label "MHC:ligand complex" ;
  rdfs:comment "A complex of molecular entities which is the result of a MHC molecule binding with a ligand." ;
  rdfs:subClassOf obo:COB_0000080 .

obo:ECO_0008080 a rdfs:Class ;
  rdfs:label "MHC:ligand binding based MHC restriction evidence" ;
  rdfs:comment "A type of protein binding evidence that employs an assay measuring binding of an MHC molecule to a ligand to determine that T cells can recognize the ligand when presented by that MHC molecule." ;
  rdfs:subClassOf obo:ECO_0000024 .

obo:GO_0140747 a rdfs:Class ;
  rdfs:label "regulation of ncRNA transcription" ;
  rdfs:subClassOf obo:GO_0006355 , obo:GO_0065007 .

obo:ECO_0007580 a rdfs:Class ;
  rdfs:label "transient rescue experiment evidence used in automatic assertion" ;
  rdfs:comment "A type of transient rescue experiment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000038 , obo:ECO_0007578 .

obo:ECO_0007473 a rdfs:Class ;
  rdfs:label "ectopic expression evidence used in automatic assertion" ;
  rdfs:comment "A type of ectopic expression evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000017 , obo:ECO_0007635 .

obo:GO_0042868 a rdfs:Class ;
  rdfs:label "antisense RNA metabolic process" ;
  rdfs:subClassOf obo:GO_0034660 .

obo:ECO_0007198 a rdfs:Class ;
  rdfs:label "positional similarity evidence used in automatic assertion" ;
  rdfs:comment "A type of positional similarity evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000060 , obo:ECO_0007824 .

obo:ECO_0006073 a rdfs:Class ;
  rdfs:label "immunofluorescence super resolution microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of super-resolution microscopy evidence used in a manual assertion resulting from the use of fluorescently-tagged antibodies to identify the localization of antigens in cells and tissue." ;
  rdfs:subClassOf obo:ECO_0006004 , obo:ECO_0006072 .

obo:OBI_0302876 a rdfs:Class ;
  rdfs:label "dissolved material entity"@en ;
  rdfs:comment "A material entity that has been going through a process of being put into solution" ;
  rdfs:subClassOf obo:BFO_0000040 , obo:OBI_0000576 .

obo:GO_0048584 a rdfs:Class ;
  rdfs:label "positive regulation of response to stimulus" ;
  rdfs:subClassOf obo:GO_0048518 , obo:GO_0048583 , obo:GO_0065007 .

obo:ECO_0005647 a rdfs:Class ;
  rdfs:label "isothermal titration calorimetry evidence used in manual assertion" ;
  rdfs:comment "A type of isothermal titration calorimetry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001825 , obo:ECO_0006075 .

obo:ECO_0007158 a rdfs:Class ;
  rdfs:label "obsolete visual sequence inspection evidence used in manual assertion" ;
  rdfs:comment "OBSOLETE. A type of visual sequence inspection evidence that is used in a manual assertion." .

obo:ECO_0000306 a rdfs:Class ;
  rdfs:label "inference from background scientific knowledge used in manual assertion" ;
  rdfs:comment "A type of inference from background scientific knowledge that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000001 , obo:ECO_0000305 .

obo:ECO_0006290 a rdfs:Class ;
  rdfs:label "site-directed spin-labelling electron paramagnetic resonance evidence used in automatic assertion" ;
  rdfs:comment "A type of site-directed spin-labelling electron paramagnetic resonance evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006254 , obo:ECO_0006288 .

obo:ECO_0007559 a rdfs:Class ;
  rdfs:label "pharmacological assay evidence used in automatic assertion" ;
  rdfs:comment "A type of pharmacological assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006053 , obo:ECO_0007635 .

obo:ECO_0006016 a rdfs:Class ;
  rdfs:label "author statement supported from referenced clinical study" ;
  rdfs:comment "A type of author statement supported by traceable reference in which the traceable reference is a clinical study." ;
  rdfs:subClassOf obo:ECO_0000033 .

obo:ECO_0000225 a rdfs:Class ;
  rdfs:label "chain termination sequencing evidence" ;
  rdfs:comment "A type of nucleotide sequencing assay in which DNA sequence is determined by addition of a primed DNA template to a reaction mixture, followed by addition of DNA polymerase, deoxynucleosidetriphosphates (dNTPs - one of which may be radiolabeled), and one of four di-deoxynucleotidetriphosphates (ddNTPs), after which DNA elongation is terminated and the DNA is separated by size and imaged." ;
  rdfs:subClassOf obo:ECO_0000219 .

obo:OBI_0001425 a rdfs:Class ;
  rdfs:label "protein binding site identification design" ;
  rdfs:comment "A study design that investigates protein binding sites on nucleic acids." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:ECO_0000295 a rdfs:Class ;
  rdfs:label "RNA-sequencing evidence" ;
  rdfs:comment "A type of transcript evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules." ;
  rdfs:subClassOf obo:ECO_0000009 .

obo:ECO_0001587 a rdfs:Class ;
  rdfs:label "ion trap mass spectrometry evidence used in automatic assertion" ;
  rdfs:comment "A type of ion trap mass spectrometry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001586 , obo:ECO_0007433 .

obo:PATO_0001018 a rdfs:Class ;
  rdfs:label "physical quality" ;
  rdfs:comment "A quality of a physical entity that exists through action of continuants at the physical level of organisation in relation to other entities." ;
  rdfs:subClassOf obo:PATO_0001241 .

obo:ECO_0001823 a rdfs:Class ;
  rdfs:label "x-ray crystallography evidence" ;
  rdfs:comment "A type of crystallography evidence where a purified sample at high concentration is crystallised and the crystals are exposed to an x ray beam to obtain three dimensional molecular structure of proteins and biological macromolecules." ;
  rdfs:subClassOf obo:ECO_0001034 .

obo:OBI_0000153 a rdfs:Class ;
  rdfs:label "cell co-culturing"@en ;
  rdfs:comment "A material combination in which cell cultures of two or more different types are are combined and allowed to culture as one."@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0005568 a rdfs:Class ;
  rdfs:label "obsolete steady state inactivation curve evidence" .

obo:ECO_0007500 a rdfs:Class ;
  rdfs:label "beta-galactosidase reporter gene assay evidence used in automatic assertion" ;
  rdfs:comment "A type of beta-galactosidase reporter gene assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001802 , obo:ECO_0007765 .

obo:ECO_0006245 a rdfs:Class ;
  rdfs:label "colony boundary assay evidence used in manual assertion" ;
  rdfs:comment "A type of colony boundary assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006240 , obo:ECO_0007078 .

obo:GO_1905952 a rdfs:Class ;
  rdfs:label "regulation of lipid localization" ;
  rdfs:subClassOf obo:GO_0032879 , obo:GO_0065007 .

obo:ECO_0007031 a rdfs:Class ;
  rdfs:label "compement fixation assay evidence" ;
  rdfs:comment "A type of immunological assay evidence that results in the detection of presence of specific antibodies in the patient's serum based on the use of complement, a biologically labile serum factor." ;
  rdfs:subClassOf obo:ECO_0000040 .

obo:ECO_0000284 a rdfs:Class ;
  rdfs:label "protein expression evidence used in manual assertion" ;
  rdfs:comment "A type of protein expression analysis that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000010 , obo:ECO_0000270 .

obo:ECO_0006010 a rdfs:Class ;
  rdfs:label "mRNA interactome capture evidence" ;
  rdfs:comment "A type of nucleic acid binding evidence in which RNA-binding proteins are identified through cross-linking RNA and proteins by UV light, capturing RNA-protein complexes on oligo(dT) beads, and finally identifying by mass spectrometry." ;
  rdfs:subClassOf obo:ECO_0000136 .

obo:ECO_0008173 a rdfs:Class ;
  rdfs:label "inference of MHC restriction based on shared MHC expression used in manual assertion" ;
  rdfs:comment "A type of inference of MHC restriction based on shared MHC expression that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007751 , obo:ECO_0008082 .

obo:GO_0051129 a rdfs:Class ;
  rdfs:label "negative regulation of cellular component organization" ;
  rdfs:subClassOf obo:GO_0048523 , obo:GO_0051128 , obo:GO_0065007 .

obo:ECO_0007606 a rdfs:Class ;
  rdfs:label "plaque assay evidence used in automatic assertion" ;
  rdfs:comment "A type of plaque assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007039 , obo:ECO_0007778 .

obo:ECO_0008035 a rdfs:Class ;
  rdfs:label "author inference based on intrinsic disorder prediction used in manual assertion" ;
  rdfs:comment "A type of author inference based on intrinsic disorder prediction that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000244 , obo:ECO_0006216 , obo:ECO_0008031 .

obo:OBI_0600046 a rdfs:Class ;
  rdfs:label "preparative chromatography"@en ;
  rdfs:comment "the use of a biomaterial's preferential affinity for either the mobile phase or the stationary phase to separate it from other materials of differing affinity"@en ;
  rdfs:subClassOf obo:OBI_0600014 .

obo:GO_0040011 a rdfs:Class ;
  rdfs:label "locomotion" ;
  rdfs:subClassOf obo:GO_0008150 .

obo:ECO_0006171 a rdfs:Class ;
  rdfs:label "quantitative western immunoblotting evidence used in automatic assertion" ;
  rdfs:comment "A type of quantitatative western immunoblotting evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006169 , obo:ECO_0007306 .

obo:ECO_0006364 a rdfs:Class ;
  rdfs:label "stopped-flow fluorescence spectroscopy evidence used in manual assertion" ;
  rdfs:comment "A type of stopped-flow fluorescence spectroscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000353 , obo:ECO_0001249 , obo:ECO_0006337 .

obo:ECO_0007633 a rdfs:Class ;
  rdfs:label "transcriptional activation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of transcriptional activation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000170 , obo:ECO_0007768 .

obo:ECO_0000231 a rdfs:Class ;
  rdfs:label "quantitative polymerase chain reaction evidence" ;
  rdfs:comment "A type of DNA detection assay evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes or fluorescent probes for detection and quantification of specific sequences in a DNA sample." ;
  rdfs:subClassOf obo:ECO_0005519 .

obo:ECO_0006166 a rdfs:Class ;
  rdfs:label "nuclear magnetic resonance imaging evidence" ;
  rdfs:comment "A type of nuclear magnetic resonance evidence used to image anatomy and physiological processes." ;
  rdfs:subClassOf obo:ECO_0001105 .

obo:GO_0006725 a rdfs:Class ;
  rdfs:label "cellular aromatic compound metabolic process" ;
  rdfs:subClassOf obo:GO_0044237 .

obo:ECO_0000242 a rdfs:Class ;
  rdfs:label "tissue ablation phenotypic evidence" ;
  rdfs:comment "A type of anatomical perturbation phenotypic evidence resulting from the surgical removal of tissue or subcellular components." ;
  rdfs:subClassOf obo:ECO_0000240 .

obo:PR_000025471 a rdfs:Class ;
  rdfs:label "nuclease S1" ;
  rdfs:comment "A protein that is a translation product of the Aspergillus oryzae RIB40 nucS gene or a 1:1 ortholog thereof." ;
  rdfs:subClassOf obo:PR_000000001 .

obo:OBI_0000883 a rdfs:Class ;
  rdfs:label "in vivo cell killing assay" ;
  rdfs:comment "A cell killing assay that measures if and how many target cells are killed within an organism." ;
  rdfs:subClassOf obo:OBI_0000882 .

obo:GO_0008283 a rdfs:Class ;
  rdfs:label "cell population proliferation" ;
  rdfs:comment "The multiplication or reproduction of cells, resulting in the expansion of a cell population." ;
  rdfs:subClassOf obo:GO_0009987 .

obo:OBI_0001151 a rdfs:Class ;
  rdfs:label "genetically modified material"@en ;
  rdfs:comment "a material entity, organism or cell,  that is the output of a genetic transformation process. "@en ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:GO_0045934 a rdfs:Class ;
  rdfs:label "negative regulation of nucleobase-containing compound metabolic process" ;
  rdfs:subClassOf obo:GO_0019219 , obo:GO_0031324 , obo:GO_0051172 , obo:GO_0065007 .

obo:ECO_0008076 a rdfs:Class ;
  rdfs:label "antibody Y-3-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody Y-3 to determine that theH2-Kb protein complex, H2-Ks protein complex, and/or H2-Kq protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0005579 a rdfs:Class ;
  rdfs:label "immunogold labelling evidence used in manual assertion" ;
  rdfs:comment "A type of immunogold labelling evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000090 , obo:ECO_0006031 .

obo:ECO_0007387 a rdfs:Class ;
  rdfs:label "fluorescence resonance energy transfer evidence used in automatic assertion" ;
  rdfs:comment "A type of fluorescence resonance energy transfer evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001048 , obo:ECO_0006328 .

obo:ECO_0008144 a rdfs:Class ;
  rdfs:label "antibody M5/114-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody M5/114-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008067 , obo:ECO_0008088 .

obo:ECO_0007563 a rdfs:Class ;
  rdfs:label "cell-free assay evidence used in automatic assertion" ;
  rdfs:comment "A type of cell-free assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000183 , obo:ECO_0007307 .

obo:ECO_0001106 a rdfs:Class ;
  rdfs:label "nuclease protection assay evidence" ;
  rdfs:comment "A type of RNA detection assay evidence resulting from the detection and quantitation of specific RNAs (from a sample of total cellular RNA) that are bound by antisense RNA or DNA probes and therefore protected from nucleases." ;
  rdfs:subClassOf obo:ECO_0005518 .

obo:ECO_0007468 a rdfs:Class ;
  rdfs:label "enzymatic activity assay evidence used in automatic assertion" ;
  rdfs:comment "A type of enzyme assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000005 , obo:ECO_0007307 .

obo:ECO_0001132 a rdfs:Class ;
  rdfs:label "tryptic phosphopeptide mapping assay evidence" ;
  rdfs:comment "A type of mass spectrometry evidence resulting from the analysis of prepared phosphopeptides separated in two dimensions on a TLC plate." ;
  rdfs:subClassOf obo:ECO_0001096 .

obo:ECO_0008073 a rdfs:Class ;
  rdfs:label "antibody SPVL3-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody SPVL3 to determine that the human MHC class II protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:OBI_0302884 a rdfs:Class ;
  rdfs:label "extraction"@en ;
  rdfs:comment "A material separation in which a desired component of an input material is separated from the remainder" ;
  rdfs:subClassOf obo:OBI_0600014 .

obo:ECO_0007585 a rdfs:Class ;
  rdfs:label "temperature-sensitive mutant phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of temperature-sensitive mutant phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006098 , obo:ECO_0007325 .

obo:ECO_0008132 a rdfs:Class ;
  rdfs:label "antibody ILA-21-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody ILA-21-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008061 , obo:ECO_0008088 .

obo:ECO_0008181 a rdfs:Class ;
  rdfs:label "T cell type based MHC restriction evidence used in manual assertion" ;
  rdfs:comment "A type of T cell type based MHC restriction evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0008086 .

obo:OBI_0600025 a rdfs:Class ;
  rdfs:label "substance detection assay" ;
  rdfs:comment "An assay that detects a specified substance" ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:GO_0007576 a rdfs:Class ;
  rdfs:label "nucleolar fragmentation" ;
  rdfs:subClassOf obo:GO_0007000 .

obo:ECO_0007635 a rdfs:Class ;
  rdfs:label "experimental phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of experimental phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000059 , obo:ECO_0007297 .

obo:ECO_0008165 a rdfs:Class ;
  rdfs:label "protein expression-based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of protein expression-based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000284 , obo:ECO_0008078 .

obo:GO_0043066 a rdfs:Class ;
  rdfs:label "negative regulation of apoptotic process" ;
  rdfs:subClassOf obo:GO_0042981 , obo:GO_0043069 , obo:GO_0065007 .

obo:ECO_0007254 a rdfs:Class ;
  rdfs:label "plasmid maintenance assay evidence used in automatic assertion" ;
  rdfs:comment "A type of plasmid maintenance assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000340 , obo:ECO_0007297 .

obo:GO_0031342 a rdfs:Class ;
  rdfs:label "negative regulation of cell killing" ;
  rdfs:subClassOf obo:GO_0031341 , obo:GO_0048523 , obo:GO_0065007 .

obo:BFO_0000029 a rdfs:Class ;
  rdfs:label "site"@en ;
  rdfs:subClassOf obo:BFO_0000141 .

obo:ECO_0007498 a rdfs:Class ;
  rdfs:label "two-dimensional polyacrylamide gel electrophoresis evidence used in automatic assertion" ;
  rdfs:comment "A type of two-dimensional polyacrylamide gel electrophoresis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005503 , obo:ECO_0007769 .

obo:ECO_0007591 a rdfs:Class ;
  rdfs:label "radioligand binding assay evidence used in automatic assertion" ;
  rdfs:comment "A type of radioligand binding assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007008 , obo:ECO_0007373 .

obo:ECO_0008191 a rdfs:Class ;
  rdfs:label "proximity-dependent biotin identification evidence used in automatic assertion" ;
  rdfs:comment "A type of proximity-dependent biotin identification evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008184 , obo:ECO_0008189 .

obo:GO_0051346 a rdfs:Class ;
  rdfs:label "negative regulation of hydrolase activity" ;
  rdfs:subClassOf obo:GO_0043086 , obo:GO_0051336 , obo:GO_0065007 .

obo:GO_2001022 a rdfs:Class ;
  rdfs:label "positive regulation of response to DNA damage stimulus" ;
  rdfs:subClassOf obo:GO_0048522 , obo:GO_0048584 , obo:GO_0065007 , obo:GO_2001020 .

obo:GO_0060255 a rdfs:Class ;
  rdfs:label "regulation of macromolecule metabolic process" ;
  rdfs:subClassOf obo:GO_0019222 , obo:GO_0065007 .

obo:ECO_0005658 a rdfs:Class ;
  rdfs:label "position-specific scoring matrix motif search evidence used in manual assertion" ;
  rdfs:comment "A type of position-specific scoring matrix motif search evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000257 , obo:ECO_0005536 .

obo:OBI_0000398 a rdfs:Class ;
  rdfs:label "image creation device" ;
  rdfs:comment "An image creation device is a device which captures a digitized image of an object" ;
  rdfs:subClassOf obo:OBI_0000832 , obo:OBI_0000968 .

obo:ECO_0005502 a rdfs:Class ;
  rdfs:label "ex vivo assay evidence" ;
  rdfs:comment "A type of direct assay evidence derived by studying an organ, tissues, or cells taken from an organism and studied in an external environment with the minimum alteration of natural conditions." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:GO_0010604 a rdfs:Class ;
  rdfs:label "positive regulation of macromolecule metabolic process" ;
  rdfs:subClassOf obo:GO_0009893 , obo:GO_0060255 , obo:GO_0065007 .

obo:ECO_0000084 a rdfs:Class ;
  rdfs:label "gene neighbors evidence" ;
  rdfs:comment "A type of genomic context evidence in which a gene product's identity is supported on the basis of the identity of neighboring genes." ;
  rdfs:subClassOf obo:ECO_0000177 .

obo:GO_0044030 a rdfs:Class ;
  rdfs:label "regulation of DNA methylation" ;
  rdfs:comment "Any process that modulates the frequency, rate or extent of the covalent transfer of a methyl group to either N-6 of adenine or C-5 or N-4 of cytosine." ;
  rdfs:subClassOf obo:GO_0040030 , obo:GO_0051052 , obo:GO_0065007 .

obo:ECO_0001233 a rdfs:Class ;
  rdfs:label "motility wound healing assay evidence used in manual assertion" ;
  rdfs:comment "A type of motility wound healing assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001099 , obo:ECO_0007695 .

obo:GO_0043604 a rdfs:Class ;
  rdfs:label "amide biosynthetic process" ;
  rdfs:subClassOf obo:GO_0043603 , obo:GO_0044271 .

obo:ECO_0001082 a rdfs:Class ;
  rdfs:label "in vivo methylation assay evidence" ;
  rdfs:comment "A type of methylation assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001554 .

obo:ECO_0007176 a rdfs:Class ;
  rdfs:label "cell-attached single-channel recording evidence used in manual assertion" ;
  rdfs:comment "A type of cell-attached single-channel recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005573 , obo:ECO_0006013 .

obo:ECO_0006259 a rdfs:Class ;
  rdfs:label "Raman spectroscopy evidence" ;
  rdfs:comment "A type of spectrophotometry evidence used to determine a structural fingerprint of the molecule in solution by probing its vibrational modes via the inelastic scattering of photons (called Raman scattering)." ;
  rdfs:subClassOf obo:ECO_0001126 , obo:ECO_0005031 .

obo:ECO_0005572 a rdfs:Class ;
  rdfs:label "whole-cell voltage clamp recording evidence" ;
  rdfs:comment "A type of voltage clamp recording evidence that involves direct measurement of ionic current across a membrane while controlling the membrane potential by maintaining the voltage." ;
  rdfs:subClassOf obo:ECO_0005576 .

obo:GO_0003964 a rdfs:Class ;
  rdfs:label "RNA-directed DNA polymerase activity" ;
  rdfs:comment "Catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Catalyzes RNA-template-directed extension of the 3'- end of a DNA strand by one deoxynucleotide at a time." ;
  rdfs:subClassOf obo:GO_0003824 .

obo:ECO_0001838 a rdfs:Class ;
  rdfs:label "in situ hybridization evidence used in manual assertion" ;
  rdfs:comment "A type of in situ hybridization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000270 , obo:ECO_0001836 .

obo:GO_0034765 a rdfs:Class ;
  rdfs:label "regulation of ion transmembrane transport" ;
  rdfs:subClassOf obo:GO_0034762 , obo:GO_0043269 , obo:GO_0065007 .

obo:ECO_0007415 a rdfs:Class ;
  rdfs:label "in vivo desumoylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo desumoylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001080 , obo:ECO_0007802 .

obo:ECO_0007668 a rdfs:Class ;
  rdfs:label "computational evidence used in manual assertion" ;
  rdfs:comment "A type of computational evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000352 , obo:ECO_0007672 .

obo:ECO_0001012 a rdfs:Class ;
  rdfs:label "cognitive assay phenotypic evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence resulting from behavioral phenotyping and analysis to assess cognitive functioning." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0008062 a rdfs:Class ;
  rdfs:label "antibody ILA-88-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody ILA-88 to determine that the cattle MHC class I protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0007219 a rdfs:Class ;
  rdfs:label "two-electrode voltage clamp recording evidence used in automatic assertion" ;
  rdfs:comment "A type of two-electrode voltage clamp recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000166 , obo:ECO_0007766 .

obo:ECO_0007800 a rdfs:Class ;
  rdfs:label "farnesylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of farnesylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001553 , obo:ECO_0007307 .

obo:ECO_0008196 a rdfs:Class ;
  rdfs:label "single-molecule fluorescence resonance energy transfer evidence used in manual assertion" ;
  rdfs:comment "A type of single-molecule fluorescence resonance energy transfer evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001183 , obo:ECO_0008187 .

obo:ECO_0006178 a rdfs:Class ;
  rdfs:label "synchrotron radiation circular dichroism evidence" ;
  rdfs:comment "A type of circular dichroism evidence where the source of radiation is radially accelerated particles, most often moving on a circular path in a synchrotron, typically providing lower wavelengths and therefore higher signal-to-noise ratio compared to regular circular dichroism measurements." ;
  rdfs:subClassOf obo:ECO_0006177 .

obo:OBI_0000387 a rdfs:Class ;
  rdfs:label "cool function"@en ;
  rdfs:comment "A cool function is a function to decrease the internal kinetic energy of a material below the initial kinetic energy of that type of material."@en ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:OBI_0600020 a rdfs:Class ;
  rdfs:label "histological assay" ;
  rdfs:comment "An assay that uses visual examination of cells or tissue (or images of them) to make an assessment regarding a quality of the cells or tissue. This assay can include steps of staining, imaging, and judgement." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007611 a rdfs:Class ;
  rdfs:label "phase contrast microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of phase contrast microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007049 , obo:ECO_0007435 .

obo:GO_0048638 a rdfs:Class ;
  rdfs:label "regulation of developmental growth" ;
  rdfs:subClassOf obo:GO_0040008 , obo:GO_0050793 , obo:GO_0065007 .

obo:GO_0006275 a rdfs:Class ;
  rdfs:label "regulation of DNA replication" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_2000112 .

obo:GO_0090344 a rdfs:Class ;
  rdfs:label "negative regulation of cell aging" ;
  rdfs:subClassOf obo:GO_0048523 , obo:GO_0051093 , obo:GO_0065007 , obo:GO_0090342 .

obo:ECO_0007827 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence used in automatic assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000088 , obo:ECO_0007322 .

obo:OBI_0000576 a rdfs:Class ;
  rdfs:label "scattered molecular aggregate"@en ;
  rdfs:comment "a material entity that consists of all the molecules of a specific type that are located in some bounded region and which is part of a more massive material entity that has parts that are other such aggregates"@en ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:ECO_0001081 a rdfs:Class ;
  rdfs:label "in vivo farnesylation assay evidence" ;
  rdfs:comment "A type of farnesylation assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001553 .

obo:ECO_0005583 a rdfs:Class ;
  rdfs:label "cut-open oocyte voltage clamp recording evidence" ;
  rdfs:comment "A type of voltage clamp recording evidence that involves inserting the oocyte in a chamber that separates the surface into 3 regions where the top portion of the oocyte membrane is the region that is clamped from which currents are actually recorded while the bottom portion is the region of the oocyte that is cut-open, making it possible to inject current intracellularly through a low resistance pathway." ;
  rdfs:subClassOf obo:ECO_0005576 .

obo:ECO_0006219 a rdfs:Class ;
  rdfs:label "combinatorial experimental and author inference evidence contained in single publication used in automatic assertion" ;
  rdfs:comment "A type of combinatorial experimental and author inference evidence contained in single publication that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006186 , obo:ECO_0007592 .

obo:ECO_0007847 a rdfs:Class ;
  rdfs:label "fluorescence quantification assay evidence" ;
  rdfs:comment "A type of fluorescence evidence in which fluorescent labeling is used to quantify a substance after a biochemical process or extraction from a biological sample." ;
  rdfs:subClassOf obo:ECO_0001115 .

obo:ECO_0001076 a rdfs:Class ;
  rdfs:label "in vivo deacetylation assay evidence" ;
  rdfs:comment "A type of deacetylation assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001548 .

obo:GO_0042060 a rdfs:Class ;
  rdfs:label "wound healing" ;
  rdfs:subClassOf obo:GO_0009611 .

obo:OBI_0000968 a rdfs:Class ;
  rdfs:label "device" ;
  rdfs:comment "A material entity that is designed to perform a function in a scientific investigation, but is not a reagent." ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:GO_0060560 a rdfs:Class ;
  rdfs:label "developmental growth involved in morphogenesis" ;
  rdfs:subClassOf obo:GO_0048589 .

obo:GO_0032501 a rdfs:Class ;
  rdfs:label "multicellular organismal process" ;
  rdfs:subClassOf obo:GO_0008150 .

obo:ECO_0001266 a rdfs:Class ;
  rdfs:label "transgenic organism evidence used in manual assertion" ;
  rdfs:comment "A type of transgenic organism evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001131 , obo:ECO_0006049 .

obo:ECO_0005590 a rdfs:Class ;
  rdfs:label "wide-field microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of wide-field microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001232 , obo:ECO_0005588 .

obo:GO_0042330 a rdfs:Class ;
  rdfs:label "taxis" ;
  rdfs:subClassOf obo:GO_0040011 .

obo:ECO_0007816 a rdfs:Class ;
  rdfs:label "molecule detection assay evidence used in automatic assertion" ;
  rdfs:comment "A type of molecule detection assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005516 , obo:ECO_0007297 .

obo:GO_0071407 a rdfs:Class ;
  rdfs:label "cellular response to organic cyclic compound" ;
  rdfs:subClassOf obo:GO_0014070 , obo:GO_0071310 .

obo:ECO_0008090 a rdfs:Class ;
  rdfs:label "antibody 10.3.6-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody 10.3.6-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008040 , obo:ECO_0008088 .

obo:ECO_0006252 a rdfs:Class ;
  rdfs:label "magnetic resonance evidence" ;
  rdfs:comment "A type of experimental evidence in which particles in strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting eletromagnetic signal is captured." ;
  rdfs:subClassOf obo:ECO_0000006 .

obo:ECO_0000164 a rdfs:Class ;
  rdfs:label "electrophysiology assay evidence" ;
  rdfs:comment "A type of direct assay evidence where electrical properties of cells or tissues are studied." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0006369 a rdfs:Class ;
  rdfs:label "computational structure modeling evidence used in automatic assertion" ;
  rdfs:comment "A type of computational structure modeling evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006341 , obo:ECO_0007669 , obo:ECO_0007815 .

obo:ECO_0005609 a rdfs:Class ;
  rdfs:label "capillary assay evidence" ;
  rdfs:comment "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by measuring the rate of accumulation of the cells into a capillary." ;
  rdfs:subClassOf obo:ECO_0005021 .

obo:ECO_0005614 a rdfs:Class ;
  rdfs:label "two-dimensional polyacrylamide gel electrophoresis evidence used in manual assertion" ;
  rdfs:comment "A type of two-dimensional polyacrylamide gel electrophoresis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0005503 .

obo:GO_1902494 a rdfs:Class ;
  rdfs:label "catalytic complex" ;
  rdfs:subClassOf obo:GO_0032991 .

obo:ECO_0006092 a rdfs:Class ;
  rdfs:label "transgenic rescue experiment evidence used in manual assertion" ;
  rdfs:comment "A type of functional complementation evidence that is used in a manual assertion that is used in manual assertion resulting from the introduction of a transgene to prevent, or \"rescue\" an organism from a condition." ;
  rdfs:subClassOf obo:ECO_0000013 , obo:ECO_0006091 .

obo:OBI_0001144 a rdfs:Class ;
  rdfs:label "binding constant"@en ;
  rdfs:comment "A quantitative binding datum that is expressed as a scalar number and a unit, which can be utilized in equations that model the binding process independent of the specific assay performed."@en ;
  rdfs:subClassOf obo:OBI_0003142 .

obo:ECO_0007286 a rdfs:Class ;
  rdfs:label "macropatch voltage clamp recording evidence used in automatic assertion" ;
  rdfs:comment "A type of macropatch voltage clamp recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005584 , obo:ECO_0007766 .

obo:ECO_0007836 a rdfs:Class ;
  rdfs:label "curator inference from Britannica used in automatic assertion" ;
  rdfs:comment "A type of curator inference from Britannica that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007639 , obo:ECO_0007834 .

obo:OBI_0000364 a rdfs:Class ;
  rdfs:label "ion detector" ;
  rdfs:comment "An ion detector is a device that measures and records the charge induced or current produced when an ion passes by or hits a surface.  Example: In a scanning instrument the signal produced in the detector during the course of the scan versus where the instrument is in the scan (at what m/Q) will produce a mass spectrum, a record of ions as a function of m/Q." ;
  rdfs:subClassOf obo:OBI_0000832 .

obo:ECO_0007596 a rdfs:Class ;
  rdfs:label "agglutination test evidence used in automatic assertion" ;
  rdfs:comment "A type of agglutination test evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007019 , obo:ECO_0007348 .

obo:ECO_0000124 a rdfs:Class ;
  rdfs:label "fusion protein localization evidence" ;
  rdfs:comment "A type of protein localization evidence resulting from the fusion of a protein of interest to a labeling protein which has enzymatic activity or fluorescence properties." ;
  rdfs:subClassOf obo:ECO_0000122 .

obo:GO_0050789 a rdfs:Class ;
  rdfs:label "regulation of biological process" ;
  rdfs:subClassOf obo:GO_0065007 .

obo:ECO_0007848 a rdfs:Class ;
  rdfs:label "fluorescence quantification assay evidence used in automatic assertion" ;
  rdfs:comment "A type of fluorescence quantification assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007450 , obo:ECO_0007847 .

obo:GO_2000191 a rdfs:Class ;
  rdfs:label "regulation of fatty acid transport" ;
  rdfs:subClassOf obo:GO_0032368 , obo:GO_0032890 , obo:GO_0065007 .

obo:ECO_0001113 a rdfs:Class ;
  rdfs:label "point mutation phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence resulting from the change in a single nucleotide." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:GO_1900117 a rdfs:Class ;
  rdfs:label "regulation of execution phase of apoptosis" ;
  rdfs:subClassOf obo:GO_0050794 , obo:GO_0065007 .

obo:ECO_0006076 a rdfs:Class ;
  rdfs:label "protein binding evidence used in manual assertion" ;
  rdfs:comment "A type of affinity evidence that is used in manual assertion resulting from the binding of a molecule to a protein or protein complex." ;
  rdfs:subClassOf obo:ECO_0000024 , obo:ECO_0006075 .

obo:OBI_0302736 a rdfs:Class ;
  rdfs:label "comet assay" ;
  rdfs:comment "An assay that measures DNA damage (DNA breakage) in eucaryotic cells exposed to a challenge by determining the size and shape of DNA migration by detecting fluorescently labeled DNA from a cell placed in an electric field using gel electrophoresis" ;
  rdfs:subClassOf obo:OBI_0001501 , obo:OBI_0001977 .

obo:GO_1901698 a rdfs:Class ;
  rdfs:label "response to nitrogen compound" ;
  rdfs:subClassOf obo:GO_0042221 .

obo:ECO_0007435 a rdfs:Class ;
  rdfs:label "microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001098 , obo:ECO_0007363 .

obo:ECO_0007723 a rdfs:Class ;
  rdfs:label "in vitro transcription reconstitution assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro transcription reconstitution assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000150 , obo:ECO_0000314 .

obo:ECO_0001548 a rdfs:Class ;
  rdfs:label "deacetylation assay evidence" ;
  rdfs:comment "A type of direct assay evidence where the removal of acetyl groups are detected in a protein." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0000154 a rdfs:Class ;
  rdfs:label "heterologous protein expression evidence" ;
  rdfs:comment "A type of protein expression evidence where a gene from one cell is inserted into a cell that does not typically contain that gene and heterologous protein expression is assessed." ;
  rdfs:subClassOf obo:ECO_0000010 .

obo:ECO_0001121 a rdfs:Class ;
  rdfs:label "radioimmunoassay evidence" ;
  rdfs:comment "A type of protein detection assay evidence resulting from the use of a radioligand to measure the binding of a substance to a specific antibody or other receptor system." ;
  rdfs:subClassOf obo:ECO_0005517 .

obo:OBI_2100023 a rdfs:Class ;
  rdfs:label "protein assay" ;
  rdfs:comment "An analyte assay that measures the abundance of protein" ;
  rdfs:subClassOf obo:OBI_0000443 .

obo:ECO_0006212 a rdfs:Class ;
  rdfs:label "particle scattering evidence used in manual assertion" ;
  rdfs:comment "A type of particle scattering evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0006183 .

obo:ECO_0007316 a rdfs:Class ;
  rdfs:label "nuclease protection assay evidence used in automatic assertion" ;
  rdfs:comment "A type of nuclease protection assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001106 , obo:ECO_0007818 .

obo:ECO_0000140 a rdfs:Class ;
  rdfs:label "thin layer chromatography evidence" ;
  rdfs:comment "A type of chromatography evidence that uses a thin layer of adsorbent (such as silica gel, alumina, or cellulose) on a flat, inert substrate." ;
  rdfs:subClassOf obo:ECO_0000325 .

obo:GO_0030030 a rdfs:Class ;
  rdfs:label "cell projection organization" ;
  rdfs:subClassOf obo:GO_0016043 .

obo:ECO_0007828 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence by experimental evidence used in automatic assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence by experimental evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005551 , obo:ECO_0007827 .

obo:ECO_0006043 a rdfs:Class ;
  rdfs:label "extracellular recording evidence" ;
  rdfs:comment "A type of electrophysiology assay evidence resulting from the use of electrodes to measure in vivo electrical activity coming from adjacent neurons." ;
  rdfs:subClassOf obo:ECO_0000164 .

obo:GO_0019815 a rdfs:Class ;
  rdfs:label "B cell receptor complex" ;
  rdfs:comment "An immunoglobulin complex that is present in the plasma membrane of B cells and that in its canonical form is composed of two identical immunoglobulin heavy chains and two identical immunoglobulin light chains and a signaling subunit, a heterodimer of the Ig-alpha and Ig-beta proteins." ;
  rdfs:subClassOf obo:OBI_1110022 .

obo:ECO_0007786 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation evidence used in automatic assertion" ;
  rdfs:comment "A type of chromatin immunoprecipitation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000226 , obo:ECO_0007503 .

obo:ECO_0007418 a rdfs:Class ;
  rdfs:label "in vivo methylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo methylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001082 , obo:ECO_0007783 .

obo:GO_0030307 a rdfs:Class ;
  rdfs:label "positive regulation of cell growth" ;
  rdfs:subClassOf obo:GO_0001558 , obo:GO_0045927 , obo:GO_0048522 , obo:GO_0065007 .

obo:OBI_0000453 a rdfs:Class ;
  rdfs:label "measure function"@en ;
  rdfs:comment "Measure function is a function that is borne by a processed material  and realized in a process in which information about some entity is expressed relative to some reference."@en ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:ECO_0007574 a rdfs:Class ;
  rdfs:label "Southwestern blot evidence used in automatic assertion" ;
  rdfs:comment "A type of Southwestern blot evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000144 , obo:ECO_0007505 .

obo:GO_0012501 a rdfs:Class ;
  rdfs:label "programmed cell death" ;
  rdfs:subClassOf obo:GO_0008219 .

obo:ECO_0000062 a rdfs:Class ;
  rdfs:label "cRNA to DNA expression microarray evidence" ;
  rdfs:comment "A type of expression microarray evidence where expression level is quantified by sample biotin-labeled cRNA (transcribed from an unknown RNA sample) hybridized to DNA oligonuclotides immoblized on a solid surface." ;
  rdfs:subClassOf obo:ECO_0000058 .

obo:ECO_0008110 a rdfs:Class ;
  rdfs:label "antibody B7/21-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody B7/21-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008050 , obo:ECO_0008088 .

obo:ECO_0006336 a rdfs:Class ;
  rdfs:label "intrinsic fluorescence-based protein conformation evidence" ;
  rdfs:comment "A type of fluorescence evidence used to study protein conformation by measuring the emissions of aromatic amino acids, specifically tryptophan, tyrosine and, to a minor extent, phenylalanine residues." ;
  rdfs:subClassOf obo:ECO_0001115 .

obo:ECO_0001120 a rdfs:Class ;
  rdfs:label "radioisotope assay evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from the assessment of biological reactions by use of a radioactive isotope to label the reactant." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0006065 a rdfs:Class ;
  rdfs:label "obsolete in vitro cell based assay evidence used in manual assertion" ;
  rdfs:comment "Use children of ECO:0001565 (cell-based assay evidence) in place of this term." .

obo:ECO_0007516 a rdfs:Class ;
  rdfs:label "luciferase reporter gene assay evidence used in automatic assertion" ;
  rdfs:comment "A type of luciferase reporter gene assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001805 , obo:ECO_0007765 .

obo:ECO_0001118 a rdfs:Class ;
  rdfs:label "protein sequencing assay evidence" ;
  rdfs:comment "A type of sequencing assay evidence resulting from determining the sequence of amino acids in a protein." ;
  rdfs:subClassOf obo:ECO_0000220 .

obo:OBI_0003120 a rdfs:Class ;
  rdfs:label "epitope:MHC:TCR complex" ;
  rdfs:comment "A complex of molecular entities which is the result of a T cell receptor binding to a MHC-epitope complex." ;
  rdfs:subClassOf obo:COB_0000080 .

obo:GO_0030262 a rdfs:Class ;
  rdfs:label "apoptotic nuclear changes" ;
  rdfs:subClassOf obo:GO_0006921 .

obo:ECO_0007103 a rdfs:Class ;
  rdfs:label "epitope-tagged protein immunolocalization evidence used in manual assertion" ;
  rdfs:comment "A type of epitope-tagged protein immunolocalization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000092 , obo:ECO_0006031 .

obo:ECO_0005675 a rdfs:Class ;
  rdfs:label "ad-hoc quantitative phenotype observation evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence where a quantifiable phenotypic trait (e.g. glucose intake) is assessed in a quantitative manner after induction of the regulatory mechanism and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0001102 a rdfs:Class ;
  rdfs:label "multiplex bead-based immunoassay evidence" ;
  rdfs:comment "A type of protein detection assay evidence resulting from determination of analyte concentration by fluorescent bead sets attached to an antibody (to a specific analyte) with a fluorescent reporter dye label attached to a second antibody (to a specific analyte)." ;
  rdfs:subClassOf obo:ECO_0005517 .

obo:ECO_0006070 a rdfs:Class ;
  rdfs:label "immuno-labelling electron microscopy evidence" ;
  rdfs:comment "A type of electron microscopy evidence resulting from the use of antibodies to identify the localization of antigens in cells and tissues." ;
  rdfs:subClassOf obo:ECO_0005033 .

obo:ECO_0008039 a rdfs:Class ;
  rdfs:label "antibody-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of protein binding evidence that employs specific antibodies to determine that a specific MHC protein complex or group of MHC protein complexes presented a ligand." ;
  rdfs:subClassOf obo:ECO_0000024 .

obo:ECO_0001067 a rdfs:Class ;
  rdfs:label "in vitro phosphatase assay evidence" ;
  rdfs:comment "A type of phosphatase assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001556 .

obo:GO_0032370 a rdfs:Class ;
  rdfs:label "positive regulation of lipid transport" ;
  rdfs:subClassOf obo:GO_0032368 , obo:GO_0051050 , obo:GO_0065007 , obo:GO_1905954 .

obo:ECO_0006054 a rdfs:Class ;
  rdfs:label "pharmacological assay evidence used in manual assertion" ;
  rdfs:comment "A type of experimental phenotypic evidence used in a manual assertion that arises from assaying the response of a cell, tissue, organ or organism following exposure to a receptor agonist or antagonist." ;
  rdfs:subClassOf obo:ECO_0006053 , obo:ECO_0007634 .

obo:ECO_0007817 a rdfs:Class ;
  rdfs:label "DNA detection assay evidence used in automatic assertion" ;
  rdfs:comment "A type of DNA detection assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005519 , obo:ECO_0007816 .

obo:ECO_0007429 a rdfs:Class ;
  rdfs:label "luminescence-based mammalian interactome mapping assay evidence used in automatic assertion" ;
  rdfs:comment "A type of luminescence-based mammalian interactome mapping assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001093 , obo:ECO_0007332 .

obo:ECO_0005670 a rdfs:Class ;
  rdfs:label "x-ray crystallography evidence used in manual assertion" ;
  rdfs:comment "A type of x-ray crystallography evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001171 , obo:ECO_0001823 .

obo:ECO_0008176 a rdfs:Class ;
  rdfs:label "T cell epitope assay type based MHC restriction evidence used in automatic assertion" ;
  rdfs:comment "A type of T cell epitope assay type based MHC restriction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007307 , obo:ECO_0008083 .

obo:ECO_0006006 a rdfs:Class ;
  rdfs:label "electrophysiology assay evidence used in manual assertion" ;
  rdfs:comment "A type of electrophysiology assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000164 , obo:ECO_0000314 .

obo:GO_0032412 a rdfs:Class ;
  rdfs:label "regulation of ion transmembrane transporter activity" ;
  rdfs:subClassOf obo:GO_0022898 , obo:GO_0034765 , obo:GO_0065007 .

obo:GO_0000785 a rdfs:Class ;
  rdfs:label "chromatin" ;
  rdfs:comment "The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome." ;
  rdfs:subClassOf obo:GO_0005575 .

obo:ECO_0007060 a rdfs:Class ;
  rdfs:label "confocal laser scanning microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of confocal laser scanning microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005589 , obo:ECO_0007059 .

obo:OBI_0001404 a rdfs:Class ;
  rdfs:label "genetic characteristics information"@en ;
  rdfs:comment "a data item that is about genetic material including polymorphisms, disease alleles, and haplotypes."@en ;
  rdfs:subClassOf obo:IAO_0000027 .

obo:ECO_0006325 a rdfs:Class ;
  rdfs:label "viscosity measurement evidence used in manual assertion" ;
  rdfs:comment "A type of viscosity measurement evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0006272 .

obo:OBI_0600036 a rdfs:Class ;
  rdfs:label "establishing cell culture" ;
  rdfs:comment "a process through which a new type of cell culture or cell line is created, either through the isolation and culture of one or more cells from a fresh source, or the deliberate experimental modification of an existing cell culture (e.g passaging a primary culture to become a secondary culture or line, or the immortalization or stable genetic modification of an existing culture or line)." ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0007641 a rdfs:Class ;
  rdfs:label "curator inference from dictionary" ;
  rdfs:comment "A type of curator inference from published work in which the entry comes from a collection of words with definitions, usages, pronounciations, and more." ;
  rdfs:subClassOf obo:ECO_0007647 .

obo:ECO_0007634 a rdfs:Class ;
  rdfs:label "experimental phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of experimental phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000059 , obo:ECO_0000269 .

obo:OBI_0000630 a rdfs:Class ;
  rdfs:label "hematology assay" ;
  rdfs:comment "An assay that studies blood and blood producing organs using a variety of techniques and instruments" ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007187 a rdfs:Class ;
  rdfs:label "tumble frequency assay evidence used in manual assertion" ;
  rdfs:comment "A type of tumble frequency assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005608 , obo:ECO_0007701 .

obo:ECO_0000241 a rdfs:Class ;
  rdfs:label "environmental perturbation phenotypic evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence resulting from modification to the surroundings or conditions in which an organism lives or operates." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0001001 a rdfs:Class ;
  rdfs:label "3D cell culture evidence" ;
  rdfs:comment "A type of cell proliferation assay evidence resulting from the growth of cells in a micro-environment that mimics real tissue and establishes physiological cell-cell and cell-substrate interactions that regulate proliferation and differentiation." ;
  rdfs:subClassOf obo:ECO_0005007 .

obo:GO_0045893 a rdfs:Class ;
  rdfs:label "positive regulation of transcription, DNA-templated" ;
  rdfs:subClassOf obo:GO_0006355 , obo:GO_0065007 , obo:GO_1903508 .

obo:OBI_0002167 a rdfs:Class ;
  rdfs:label "subcellular protein localization assay" ;
  rdfs:comment "A protein localization assay that determines the specific subcellular location of a protein. The location is visualized through electron microscopy." ;
  rdfs:subClassOf obo:OBI_0002165 .

obo:OBI_0000678 a rdfs:Class ;
  rdfs:label "portioning objective" ;
  rdfs:comment "A material separation objective aiming to separate material into multiple portions, each of which contains a similar composition of the input material." ;
  rdfs:subClassOf obo:OBI_0000639 .

obo:ECO_0007265 a rdfs:Class ;
  rdfs:label "nonsense mutation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of nonsense mutation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005507 , obo:ECO_0007448 .

obo:ECO_0007489 a rdfs:Class ;
  rdfs:label "high throughput mass spectrometry evidence used in automatic assertion" ;
  rdfs:comment "A type of high throughput mass spectrometry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005585 , obo:ECO_0007433 .

obo:OBI_2000009 a rdfs:Class ;
  rdfs:label "bodily fluid specimen" ;
  rdfs:comment "A specimen that is derived from some bodily fluid" ;
  rdfs:subClassOf obo:OBI_0001479 .

obo:ECO_0007837 a rdfs:Class ;
  rdfs:label "curator inference from MedlinePlus encyclopedia used in automatic assertion" ;
  rdfs:comment "A type of curator inference from MedlinePlus encyclopedia that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007640 , obo:ECO_0007834 .

obo:GO_0044260 a rdfs:Class ;
  rdfs:label "cellular macromolecule metabolic process" ;
  rdfs:subClassOf obo:GO_0043170 , obo:GO_0044237 .

obo:ECO_0000009 a rdfs:Class ;
  rdfs:label "transcript expression evidence" ;
  rdfs:comment "A type of expression pattern evidence where abundance of a transcript is analyzed." ;
  rdfs:subClassOf obo:ECO_0000008 .

obo:ECO_0005594 a rdfs:Class ;
  rdfs:label "immunoperoxidase immunolocalization evidence" ;
  rdfs:comment "A type of immunolocalization evidence involving enzymatic detection of a peroxidase tagged antibody." ;
  rdfs:subClassOf obo:ECO_0000087 .

obo:ECO_0001124 a rdfs:Class ;
  rdfs:label "restriction fragment detection evidence" ;
  rdfs:comment "A type of DNA detection assay evidence resulting from homologous DNA fragments digested by restriction enzymes, and the resulting restriction fragments are sorted by length to illustrate differences." ;
  rdfs:subClassOf obo:ECO_0005519 .

obo:ECO_0006081 a rdfs:Class ;
  rdfs:label "far-Western blotting evidence used in manual assertion" ;
  rdfs:comment "A type of physical interaction evidence that is used in a manual assertion that is based on detection of protein-protein interactions by separation of target proteins by SDS-PAGE which are blotted to a membrane, followed by denaturation and renaturation, probing with purified bait proteins, and detection of the target-bait complexes." ;
  rdfs:subClassOf obo:ECO_0000076 , obo:ECO_0000353 .

obo:OBI_0400078 a rdfs:Class ;
  rdfs:label "obscuration bar" ;
  rdfs:comment "An obscuration bar is a an optical subsystem which is a strip of metal or other material that serves to block out direct light from the illuminating beam. The obscuration bar prevents the bright light scattered in the forward directions from burning out the collection device." ;
  rdfs:subClassOf obo:OBI_0000932 .

obo:ECO_0007394 a rdfs:Class ;
  rdfs:label "in vitro protein cleavage assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro protein cleavage assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001058 , obo:ECO_0007782 .

obo:ECO_0005643 a rdfs:Class ;
  rdfs:label "hydroxyl-radical footprinting evidence used in manual assertion" ;
  rdfs:comment "A type of hydroxyl-radical footprinting evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001818 , obo:ECO_0006083 .

obo:OBI_0600000 a rdfs:Class ;
  rdfs:label "adding substance to cell culture"@en ;
  rdfs:comment "is process in which a material substance is added to a cell culture"@en ;
  rdfs:subClassOf obo:OBI_0000274 .

obo:ECO_0007736 a rdfs:Class ;
  rdfs:label "molecule detection assay evidence used in manual assertion" ;
  rdfs:comment "A type of molecule detection assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0005516 .

obo:GO_1903353 a rdfs:Class ;
  rdfs:label "regulation of nucleus organization" ;
  rdfs:subClassOf obo:GO_0033043 , obo:GO_0065007 .

obo:ECO_0008084 a rdfs:Class ;
  rdfs:label "MHC-specific blocking based MHC restriction evidence" ;
  rdfs:comment "A type of protein binding evidence that employs the specific blocking of MHC molecules to determine that a ligand is restricted by that MHC molecule or class of MHC molecules." ;
  rdfs:subClassOf obo:ECO_0000024 .

obo:ECO_0007457 a rdfs:Class ;
  rdfs:label "restriction fragment detection evidence used in automatic assertion" ;
  rdfs:comment "A type of restriction fragment detection evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001124 , obo:ECO_0007817 .

obo:OBI_0000366 a rdfs:Class ;
  rdfs:label "metabolite profiling assay" ;
  rdfs:comment "An assay that detects and identifies chemical entities resulting from biochemical and cellular metabolism" ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0006392 a rdfs:Class ;
  rdfs:label "bait-prey interaction evidence used in manual assertion" ;
  rdfs:comment "A type of bait-prey interaction evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006076 , obo:ECO_0006351 .

obo:ECO_0007533 a rdfs:Class ;
  rdfs:label "super-resolution microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of super-resolution microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005578 , obo:ECO_0007435 .

obo:GO_1990837 a rdfs:Class ;
  rdfs:label "sequence-specific double-stranded DNA binding" ;
  rdfs:subClassOf obo:GO_0003690 , obo:GO_0043565 .

obo:ECO_0005530 a rdfs:Class ;
  rdfs:label "random mutagenesis evidence used in manual assertion" ;
  rdfs:comment "A type of random mutagenesis phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0005529 .

obo:OBI_0001177 a rdfs:Class ;
  rdfs:label "RNA sequencing assay" ;
  rdfs:comment "An assay that determines the sequence of an RNA molecule." ;
  rdfs:subClassOf obo:OBI_0600047 .

obo:ECO_0000181 a rdfs:Class ;
  rdfs:label "in vitro assay evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from a process performed outside the living organism, where the components of an organism have been isolated from their usual biological context, to permit a more detailed or more convenient analysis in an artificial setting." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0001827 a rdfs:Class ;
  rdfs:label "methylation interference footprinting evidence" ;
  rdfs:comment "A type of nucleic acid binding evidence used to identify protein binding sites on the DNA molecule where multiple copies of a DNA fragment containing a putative TF-binding site are randomly methylated with dimethyl sulfate (DMS) and cleaved at the methyl group, followed by separating the cleavage products on a denaturing electrophoresis gel." ;
  rdfs:subClassOf obo:ECO_0000136 .

obo:ECO_0006236 a rdfs:Class ;
  rdfs:label "protein hydrogen-deuterium exchange mass spectrometry evidence used in manual assertion" ;
  rdfs:comment "A type of protein hydrogen-deuterium exchange mass spectrometry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001230 , obo:ECO_0006195 .

obo:OBI_0001247 a rdfs:Class ;
  rdfs:label "genotyping by high throughput sequencing assay" ;
  rdfs:comment "A genotyping assay that uses a high througput sequencer" ;
  rdfs:subClassOf obo:OBI_0000435 .

obo:GO_0009056 a rdfs:Class ;
  rdfs:label "catabolic process" ;
  rdfs:subClassOf obo:GO_0008152 .

obo:ECO_0008091 a rdfs:Class ;
  rdfs:label "antibody 14.4.4-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody 14.4.4-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008041 , obo:ECO_0008087 .

obo:ECO_0006241 a rdfs:Class ;
  rdfs:label "galactokinase reporter gene assay evidence used in manual assertion" ;
  rdfs:comment "A type of galactokinase reporter gene assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000270 , obo:ECO_0006238 .

obo:OBI_0000684 a rdfs:Class ;
  rdfs:label "specimen collection objective" ;
  rdfs:comment "A objective specification to obtain a material entity for potential use as an input during an investigation." ;
  rdfs:subClassOf obo:IAO_0000005 .

obo:GO_0006417 a rdfs:Class ;
  rdfs:label "regulation of translation" ;
  rdfs:subClassOf obo:GO_0010608 , obo:GO_0032268 , obo:GO_0034248 , obo:GO_0065007 , obo:GO_2000112 .

obo:ECO_0006312 a rdfs:Class ;
  rdfs:label "protein unfolding evidence used in automatic assertion" ;
  rdfs:comment "A type of protein unfolding evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006265 , obo:ECO_0007815 .

obo:GO_1904747 a rdfs:Class ;
  rdfs:label "positive regulation of apoptotic process involved in development" ;
  rdfs:subClassOf obo:GO_0043065 , obo:GO_0051094 , obo:GO_0065007 , obo:GO_1904748 .

obo:ECO_0008019 a rdfs:Class ;
  rdfs:label "support vector machine evidence used in automatic assertion" ;
  rdfs:comment "A type of support vector machine evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008004 , obo:ECO_0008010 .

obo:ECO_0008121 a rdfs:Class ;
  rdfs:label "antibody DK1G8-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody DK1G8-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008056 , obo:ECO_0008087 .

obo:ECO_0008005 a rdfs:Class ;
  rdfs:label "deep learning neural network method evidence used in manual assertion" ;
  rdfs:comment "A type of deep learning neural network method evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008001 , obo:ECO_0008024 .

obo:GO_0044265 a rdfs:Class ;
  rdfs:label "cellular macromolecule catabolic process" ;
  rdfs:subClassOf obo:GO_0009057 , obo:GO_0044248 , obo:GO_0044260 .

obo:GO_0009719 a rdfs:Class ;
  rdfs:label "response to endogenous stimulus" ;
  rdfs:subClassOf obo:GO_0050896 .

obo:OBI_0001108 a rdfs:Class ;
  rdfs:label "nucleic acid sequencer" ;
  rdfs:comment "An device that is used to determine the order of nucleotides in nucleic acid sequences." ;
  rdfs:subClassOf obo:OBI_0000832 .

obo:ECO_0001559 a rdfs:Class ;
  rdfs:label "sumoylation assay evidence" ;
  rdfs:comment "A type of direct assay evidence where sumoylated residues on a protein are detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007544 a rdfs:Class ;
  rdfs:label "colony biofilm assay evidence used in automatic assertion" ;
  rdfs:comment "A type of colony biofilm assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006026 , obo:ECO_0007541 .

obo:ECO_0006201 a rdfs:Class ;
  rdfs:label "circular dichroism evidence used in automatic assertion" ;
  rdfs:comment "A type of circular dichroism evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006177 , obo:ECO_0007815 , obo:ECO_0007821 .

obo:ECO_0005556 a rdfs:Class ;
  rdfs:label "multiple sequence alignment evidence used in manual assertion" ;
  rdfs:comment "A type of multiple sequence alignment evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000247 , obo:ECO_0005555 .

obo:ECO_0008180 a rdfs:Class ;
  rdfs:label "specific MHC molecule based MHC restriction evidence used in automatic assertion" ;
  rdfs:comment "A type of specific MHC molecule based MHC restriction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007307 , obo:ECO_0008085 .

obo:OBI_0000377 a rdfs:Class ;
  rdfs:label "perturb function"@en ;
  rdfs:comment "A perturb function is a function that disrupts the normal function of a system induced through either internal or external means.  External means of perturbation include: (1) displacement fields in the physical sense - e.g., temperature change, osmotic shock, pressure change; (2) application of small molecules such as drugs or toxins to perturb the function of specific pathways or application of surfactants to perturb the normal function of plasma membrane.  Internal means of perturbation include: (1) manipulation of gene function via gene knockout or transcript knockdown via RNAi; (2) directed genetic mutation leading to minimal aa alterations that interfere with peptide function."@en ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:GO_0010629 a rdfs:Class ;
  rdfs:label "negative regulation of gene expression" ;
  rdfs:subClassOf obo:GO_0010468 , obo:GO_0010605 , obo:GO_0065007 .

obo:ECO_0007275 a rdfs:Class ;
  rdfs:label "current density evidence used in automatic assertion" ;
  rdfs:comment "A type of current density evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005564 , obo:ECO_0007535 .

obo:ECO_0007131 a rdfs:Class ;
  rdfs:label "5C evidence used in manual assertion" ;
  rdfs:comment "A type of 5C evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000235 , obo:ECO_0000269 .

obo:ECO_0000276 a rdfs:Class ;
  rdfs:label "cRNA to DNA expression microarray evidence used in manual assertion" ;
  rdfs:comment "A type of cRNA to DNA expression microarray evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000062 , obo:ECO_0000275 .

obo:GO_0060354 a rdfs:Class ;
  rdfs:label "negative regulation of cell adhesion molecule production" ;
  rdfs:subClassOf obo:GO_0048523 , obo:GO_0060353 , obo:GO_0065007 .

obo:ECO_0008124 a rdfs:Class ;
  rdfs:label "antibody DT9-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody DT9-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008057 , obo:ECO_0008088 .

obo:ECO_0007370 a rdfs:Class ;
  rdfs:label "knockout evidence used in automatic assertion" ;
  rdfs:comment "A type of knockout evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001091 , obo:ECO_0007427 .

obo:GO_1903626 a rdfs:Class ;
  rdfs:label "positive regulation of DNA catabolic process" ;
  rdfs:subClassOf obo:GO_0031331 , obo:GO_0051054 , obo:GO_0065007 , obo:GO_1903624 .

obo:GO_0001882 a rdfs:Class ;
  rdfs:label "nucleoside binding" ;
  rdfs:subClassOf obo:GO_0036094 , obo:GO_0097159 , obo:GO_0097367 , obo:GO_1901363 .

obo:ECO_0007172 a rdfs:Class ;
  rdfs:label "sustained current evidence used in manual assertion" ;
  rdfs:comment "A type of sustained current evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005566 , obo:ECO_0006006 .

obo:ECO_0007023 a rdfs:Class ;
  rdfs:label "direct Coombs test evidence" ;
  rdfs:comment "A type of agglutination test evidence that results in detection of nonagglutinating antibodies or complement proteins on red blood cells in vivo." ;
  rdfs:subClassOf obo:ECO_0007019 .

obo:ECO_0007639 a rdfs:Class ;
  rdfs:label "curator inference from Britannica" ;
  rdfs:comment "A type of curator inference from encyclopedia where the reference is to an Encyclopedia Britannica article." ;
  rdfs:subClassOf obo:ECO_0007637 .

obo:GO_0006811 a rdfs:Class ;
  rdfs:label "ion transport" ;
  rdfs:subClassOf obo:GO_0006810 .

obo:ECO_0007381 a rdfs:Class ;
  rdfs:label "RNA dot blot assay evidence used in automatic assertion" ;
  rdfs:comment "A type of RNA dot blot assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001040 , obo:ECO_0007818 .

obo:ECO_0001071 a rdfs:Class ;
  rdfs:label "in vitro transcription assay evidence" ;
  rdfs:comment "A type of transcription assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0000168 .

obo:IDO_0000586 a rdfs:Class ;
  rdfs:label "infection"@en ;
  rdfs:comment "A part of an extended organism that itself has as part a population of one or more infectious agents and that (1) exists as a result of processes initiated by members of the infectious agent population and is (2) clinically abnormal in virtue of the presence of this infectious agent population, or (3) has a disposition to bring clinical abnormality to immunocompetent organisms of the same Species as the host (the organism corresponding to the extended organism) through transmission of a member or offspring of a member of the infectious agent population."@en ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:OBI_0003117 a rdfs:Class ;
  rdfs:label "3D molecular structure determination assay" ;
  rdfs:comment "An assay that determines the 3-dimensional configuration of a molecular entity or molecular complex." ;
  rdfs:subClassOf obo:OBI_0600045 .

obo:GO_0051101 a rdfs:Class ;
  rdfs:label "regulation of DNA binding" ;
  rdfs:subClassOf obo:GO_0051098 , obo:GO_0065007 .

obo:OBI_0600017 a rdfs:Class ;
  rdfs:label "assay detecting a molecular label" ;
  rdfs:comment "An assay that detects the presence or a quality of a molecular label which is a proxy for the detection of the molecular target to which the label is attached" ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0008185 a rdfs:Class ;
  rdfs:label "avidity evidence" ;
  rdfs:comment "A type of physical interaction evidence that is the accumulated strength of multiple affinities of individual non-covalent binding interactions, such as between a protein receptor and its ligand. Avidity should not be thought of as the mere sum of its constituent affinities but as the combined effect of all affinities participating in the biomolecular interaction. It is commonly referred to as functional affinity." ;
  rdfs:subClassOf obo:ECO_0000021 .

obo:ECO_0001065 a rdfs:Class ;
  rdfs:label "in vitro methylation assay evidence" ;
  rdfs:comment "A type of methylation assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001554 .

obo:ECO_0001176 a rdfs:Class ;
  rdfs:label "DNA laddering assay evidence used in manual assertion" ;
  rdfs:comment "A type of DNA laddering assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001039 , obo:ECO_0007698 .

obo:ECO_0001161 a rdfs:Class ;
  rdfs:label "chimeric protein phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of chimeric protein phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0001024 .

obo:ECO_0007811 a rdfs:Class ;
  rdfs:label "cleavage arrested development evidence used in automatic assertion" ;
  rdfs:comment "A type of cleavage arrested development evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000298 , obo:ECO_0007635 .

obo:GO_0010608 a rdfs:Class ;
  rdfs:label "posttranscriptional regulation of gene expression" ;
  rdfs:subClassOf obo:GO_0010468 .

obo:OBI_0001129 a rdfs:Class ;
  rdfs:label "voltage clamp device" ;
  rdfs:comment "A device that is used to measure the ion currents across the membrane of excitable cells, such as neurons, while holding the membrane voltage at a set level." ;
  rdfs:subClassOf obo:OBI_0000832 .

obo:ECO_0000012 a rdfs:Class ;
  rdfs:label "functional complementation evidence" ;
  rdfs:comment "A type of genetic interaction evidence where a wild-type copy of the gene in question is inserted into a mutant cell to see if it restores the wild-type phenotype in the mutant background." ;
  rdfs:subClassOf obo:ECO_0000011 .

obo:ECO_0006370 a rdfs:Class ;
  rdfs:label "structural ensemble generation evidence used in manual assertion" ;
  rdfs:comment "A type of structural ensemble generation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006339 , obo:ECO_0006368 .

obo:OBI_0000912 a rdfs:Class ;
  rdfs:label "X-ray crystallography 3D molecular structure determination assay" ;
  rdfs:comment "A 3D molecular structure determination assay in which the diffraction of pattern of X-ray beams in a crystal of purified material entities is used to resolve the 3-dimensional structure of the material entity of interest." ;
  rdfs:subClassOf obo:OBI_0003117 .

obo:ECO_0001272 a rdfs:Class ;
  rdfs:label "high-performance liquid chromatography evidence used in manual assertion" ;
  rdfs:comment "A type of high-performance liquid chromatography evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001052 , obo:ECO_0007680 .

obo:ECO_0007053 a rdfs:Class ;
  rdfs:label "koehler illumination microscopy evidence" ;
  rdfs:comment "A type of microscopy evidence that results in a bright image without glare and minimum heating of the specimen by employing both field and an aperture iris diaphragm for illumination." ;
  rdfs:subClassOf obo:ECO_0001098 .

obo:ECO_0000309 a rdfs:Class ;
  rdfs:label "cap analysis of gene expression evidence" ;
  rdfs:comment "A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by a cap-trapper system." ;
  rdfs:subClassOf obo:ECO_0000009 , obo:IAO_0000030 .

obo:OBI_0002180 a rdfs:Class ;
  rdfs:label "inside-out patch clamp assay" ;
  rdfs:comment "A patch-clamp assay where a patch of the membrane is attached to the patch pipette, detached from the rest of the cell, and the cytosolic surface of the membrane is exposed to the external media, or bath. This provides the experimenter has access to the intracellular surface of the membrane via the bath and can manipulate the environment at the intracellular surface of single ion channels. For example, channels that are activated by intracellular ligands can then be studied through a range of ligand concentrations." ;
  rdfs:subClassOf obo:OBI_0002177 .

obo:GO_0060149 a rdfs:Class ;
  rdfs:label "negative regulation of posttranscriptional gene silencing" ;
  rdfs:subClassOf obo:GO_0010628 , obo:GO_0048519 , obo:GO_0060147 , obo:GO_0065007 .

obo:ECO_0006032 a rdfs:Class ;
  rdfs:label "optogenetic evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence arising from experiment in which neuronal activity is manipulated using genetically encoded, optically activated neuronal actuators." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0001017 a rdfs:Class ;
  rdfs:label "Boyden chamber assay evidence" ;
  rdfs:comment "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by measuring the number of cells that migrate from the upper compartment of a chamber (separated by a micro-porous membrane) to the lower compartment, in which chemotactic agents are present." ;
  rdfs:subClassOf obo:ECO_0005021 .

obo:ECO_0007747 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence by experimental evidence used in manual assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence by experimental evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005551 , obo:ECO_0007746 .

obo:ECO_0006088 a rdfs:Class ;
  rdfs:label "systematic evolution of ligands by exponential amplification evidence used in manual assertion" ;
  rdfs:comment "A type of evidence that is used in a manual assertion arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand." ;
  rdfs:subClassOf obo:ECO_0000293 , obo:ECO_0000353 .

obo:OBI_0001999 a rdfs:Class ;
  rdfs:label "3D molecular structure determination assay of a T cell epitope:MHC:TCR complex" ;
  rdfs:comment "A 3D structure determination of bound molecular complex assay that characterizes the 3-dimensional structure of a T cell epitope:MHC:TCR complex." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_0001145 , obo:OBI_0003128 .

obo:ECO_0000288 a rdfs:Class ;
  rdfs:label "RNA protection assay evidence used in manual assertion" ;
  rdfs:comment "A type of RNA protection assay that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000110 , obo:ECO_0001240 .

obo:GO_0006139 a rdfs:Class ;
  rdfs:label "nucleobase-containing compound metabolic process" ;
  rdfs:subClassOf obo:GO_0006725 , obo:GO_0034641 , obo:GO_0044238 , obo:GO_0046483 , obo:GO_1901360 .

obo:ECO_0005545 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence based on orthology evidence" ;
  rdfs:comment "A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by orthology from an existing experimentally supported model to a process, pathway or complex in another species." ;
  rdfs:subClassOf obo:ECO_0005549 .

obo:PATO_0001241 a rdfs:Class ;
  rdfs:label "physical object quality" ;
  rdfs:comment "A quality which inheres in a continuant." ;
  rdfs:subClassOf obo:BFO_0000019 .

obo:ECO_0000338 a rdfs:Class ;
  rdfs:label "pulsed-field gel electrophoresis evidence" ;
  rdfs:comment "A type of gel electrophoresis evidence in which large DNA molecules are separated on agarose gel by alternately pulsed, perpendicularly oriented electrical fields, at least one of which is inhomogeneous." ;
  rdfs:subClassOf obo:ECO_0000337 .

obo:GO_0001906 a rdfs:Class ;
  rdfs:label "cell killing" ;
  rdfs:comment "Any process in an organism that results in the killing of its own cells or those of another organism, including in some cases the death of the other organism. Killing here refers to the induction of death in one cell by another cell, not cell-autonomous death due to internal or other environmental conditions." ;
  rdfs:subClassOf obo:GO_0009987 .

obo:OBI_0000064 a rdfs:Class ;
  rdfs:label "positive reference substance role"@en ;
  rdfs:comment "a reference role in which the characteristics or responses elicited by the substance playing the reference substance role are used to establish a \"100%\" response" ;
  rdfs:subClassOf obo:OBI_0000025 .

obo:GO_0048870 a rdfs:Class ;
  rdfs:label "cell motility" ;
  rdfs:subClassOf obo:GO_0006928 , obo:GO_0040011 .

obo:OBI_0000113 a rdfs:Class ;
  rdfs:label "sequence feature identification objective"@en ;
  rdfs:comment "Sequence_feature_identification_objective is a biological_feature_identification_objective role describing a study designed to examine or characterize molecular features exhibited at the level of a macromolecular sequence, e.g. nucleic acid, protein, polysaccharide."@en ;
  rdfs:subClassOf obo:OBI_0000015 .

obo:ECO_0007451 a rdfs:Class ;
  rdfs:label "protein dot blot assay evidence used in automatic assertion" ;
  rdfs:comment "A type of protein dot blot assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001116 , obo:ECO_0007819 .

obo:GO_0042127 a rdfs:Class ;
  rdfs:label "regulation of cell population proliferation" ;
  rdfs:subClassOf obo:GO_0050794 , obo:GO_0065007 .

obo:OBI_0003142 a rdfs:Class ;
  rdfs:label "quantitative binding datum" ;
  rdfs:comment "A binding datum that is expressed using numbers." ;
  rdfs:subClassOf obo:OBI_0001181 .

obo:ECO_0006386 a rdfs:Class ;
  rdfs:label "host penetration assay evidence used in manual assertion" ;
  rdfs:comment "A type of host penetration assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001832 , obo:ECO_0006348 .

obo:ECO_0007704 a rdfs:Class ;
  rdfs:label "methylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of methylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001554 .

obo:ECO_0000095 a rdfs:Class ;
  rdfs:label "cell growth regulation assay evidence" ;
  rdfs:comment "A type of cell growth assay evidence that measures one or more aspects of cell growth over a specified time period to indicate an induced or repressed regulatory effect." ;
  rdfs:subClassOf obo:ECO_0001563 .

obo:GO_0044728 a rdfs:Class ;
  rdfs:label "DNA methylation or demethylation" ;
  rdfs:subClassOf obo:GO_0006304 .

obo:ECO_0007092 a rdfs:Class ;
  rdfs:label "gain-of-function mutant phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of gain-of-function mutant phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000042 , obo:ECO_0000315 .

obo:ECO_0006306 a rdfs:Class ;
  rdfs:label "native protein gel electrophoresis evidence used in automatic assertion" ;
  rdfs:comment "A type of native protein gel electrophoresis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006262 , obo:ECO_0007769 .

obo:ECO_0006099 a rdfs:Class ;
  rdfs:label "temperature-sensitive mutant phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of temperature-sensitive mutant phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0006098 .

obo:ECO_0000236 a rdfs:Class ;
  rdfs:label "obsolete chromosome conformation capture-PCR evidence" ;
  rdfs:comment "OBSOLETE. A type of chromosome conformation capture evidence that is derived by performing polymerase chain reaction (PCR) in the quantitation step." .

obo:GO_0043603 a rdfs:Class ;
  rdfs:label "cellular amide metabolic process" ;
  rdfs:subClassOf obo:GO_0034641 .

obo:ECO_0007534 a rdfs:Class ;
  rdfs:label "fractionation evidence used in automatic assertion" ;
  rdfs:comment "A type of fractionation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000100 , obo:ECO_0007307 .

obo:ECO_0007208 a rdfs:Class ;
  rdfs:label "co-fractionation evidence used in automatic assertion" ;
  rdfs:comment "A type of co-fractionation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000102 , obo:ECO_0007534 .

obo:OBI_0002692 a rdfs:Class ;
  rdfs:label "polymerase chain reaction assay" ;
  rdfs:comment "An assay that uses polymerase chain reaction technique to amplify DNA." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007355 a rdfs:Class ;
  rdfs:label "caspase assay evidence used in automatic assertion" ;
  rdfs:comment "A type of caspase assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001019 , obo:ECO_0007777 .

obo:ECO_0007411 a rdfs:Class ;
  rdfs:label "in vivo protein cleavage assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo protein cleavage assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001075 , obo:ECO_0007782 .

obo:GO_0042981 a rdfs:Class ;
  rdfs:label "regulation of apoptotic process" ;
  rdfs:subClassOf obo:GO_0043067 , obo:GO_0065007 .

obo:ECO_0000353 a rdfs:Class ;
  rdfs:label "physical interaction evidence used in manual assertion" ;
  rdfs:comment "A type of physical interaction evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/ipi-inferred-physical-interaction> ;
  rdfs:subClassOf obo:ECO_0000021 , obo:ECO_0000314 .

obo:ECO_0007161 a rdfs:Class ;
  rdfs:label "nonsense mutation phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of nonsense mutation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001247 , obo:ECO_0005507 .

obo:IAO_0000178 a rdfs:Class ;
  rdfs:label "material information bearer"@en ;
  rdfs:comment "A material entity in which a concretization of an information content entity inheres."@en ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:ECO_0001089 a rdfs:Class ;
  rdfs:label "in vivo ubiquitination assay evidence" ;
  rdfs:comment "A type of ubiquitination assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001562 .

obo:OBI_0001199 a rdfs:Class ;
  rdfs:label "in vivo design" ;
  rdfs:comment "A study design that is conducted entirely in a living organism, e.g. a compound treatment in a mouse model." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:ECO_0007277 a rdfs:Class ;
  rdfs:label "use dependence of inactivation evidence used in automatic assertion" ;
  rdfs:comment "A type of use dependence of inactivation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005570 , obo:ECO_0007535 .

obo:ECO_0000158 a rdfs:Class ;
  rdfs:label "protein separation followed by direct sequencing evidence" ;
  rdfs:comment "A type of protein separation evidence that is followed by direct protein sequencing whereby a protein's amino acid sequence is determined experimentally by Edman degradation or by mass spectrometry." ;
  rdfs:subClassOf obo:ECO_0000156 .

obo:ECO_0007600 a rdfs:Class ;
  rdfs:label "direct hemagglutination assay evidence used in automatic assertion" ;
  rdfs:comment "A type of direct hemagglutination assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007027 , obo:ECO_0007596 .

obo:OBI_0302732 a rdfs:Class ;
  rdfs:label "solvent role"@en ;
  rdfs:comment "solvent role is a role which inheres in a molecular entity capable of ensuring the dissolution of another chemical entity and realized by the process of solvation"@en ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:ECO_0008169 a rdfs:Class ;
  rdfs:label "MHC:ligand binding based MHC restriction evidence used in manual assertion" ;
  rdfs:comment "A type of MHC:ligand binding based MHC restriction evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006076 , obo:ECO_0008080 .

obo:ECO_0006009 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation-seq evidence used in manual assertion" ;
  rdfs:comment "A type of chromatin immunoprecipitation-seq evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000229 , obo:ECO_0005644 .

obo:ECO_0001143 a rdfs:Class ;
  rdfs:label "adhesion assay evidence used in manual assertion" ;
  rdfs:comment "A type of adhesion assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001006 , obo:ECO_0007695 .

obo:GO_0051674 a rdfs:Class ;
  rdfs:label "localization of cell" ;
  rdfs:subClassOf obo:GO_0009987 , obo:GO_0051179 .

obo:ECO_0007592 a rdfs:Class ;
  rdfs:label "combinatorial experimental and author inference evidence used in automatic assertion" ;
  rdfs:comment "A type of combinatorial evidence from author knowledge and experimental evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000213 , obo:ECO_0007011 .

obo:ECO_0000327 a rdfs:Class ;
  rdfs:label "whole transcript splice pattern evidence" ;
  rdfs:comment "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features." ;
  rdfs:subClassOf obo:ECO_0000326 .

obo:ECO_0001182 a rdfs:Class ;
  rdfs:label "fluorescence in situ hybridization evidence used in manual assertion" ;
  rdfs:comment "A type of fluorescence in situ hybridization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001047 , obo:ECO_0001838 .

obo:ECO_0007401 a rdfs:Class ;
  rdfs:label "in vitro methylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro methylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001065 , obo:ECO_0007783 .

obo:GO_0051173 a rdfs:Class ;
  rdfs:label "positive regulation of nitrogen compound metabolic process" ;
  rdfs:subClassOf obo:GO_0009893 , obo:GO_0051171 , obo:GO_0065007 .

obo:GO_0045900 a rdfs:Class ;
  rdfs:label "negative regulation of translational elongation" ;
  rdfs:subClassOf obo:GO_0006448 , obo:GO_0017148 , obo:GO_0065007 .

obo:ECO_0006347 a rdfs:Class ;
  rdfs:label "host-surrogate penetration assay evidence" ;
  rdfs:comment "A type of experimental evidence in which the extent of penetration into a host-surrogate material consisting of a synthetic or other non-living substance is measured." ;
  rdfs:subClassOf obo:ECO_0000006 .

obo:GO_0006351 a rdfs:Class ;
  rdfs:label "transcription, DNA-templated" ;
  rdfs:subClassOf obo:GO_0097659 .

obo:ECO_0006359 a rdfs:Class ;
  rdfs:label "differential scanning fluorimetry evidence used in automatic assertion" ;
  rdfs:comment "A type of differential scanning fluorimetry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006302 , obo:ECO_0006334 , obo:ECO_0007450 .

obo:ECO_0001038 a rdfs:Class ;
  rdfs:label "deletion mutation phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence resulting from a mutation in which there is the removal of one or more contiguous nucleotides that may result in altered function or other measurable properties." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0001184 a rdfs:Class ;
  rdfs:label "gel-filtration evidence used in manual assertion" ;
  rdfs:comment "A type of gel-filtration evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001049 , obo:ECO_0007680 .

obo:ECO_0006407 a rdfs:Class ;
  rdfs:label "single-cell RNA-sequencing evidence used in automatic assertion" ;
  rdfs:comment "A type of single cell RNA-sequencing evidence that is used in automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001560 , obo:ECO_0006069 .

obo:BFO_0000141 a rdfs:Class ;
  rdfs:label "immaterial entity"@en ;
  rdfs:subClassOf obo:BFO_0000004 .

obo:GO_0034061 a rdfs:Class ;
  rdfs:label "DNA polymerase activity" ;
  rdfs:comment "Catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1); the synthesis of DNA from deoxyribonucleotide triphosphates in the presence of a nucleic acid template and a 3'hydroxyl group." ;
  rdfs:subClassOf obo:GO_0003824 .

obo:UBERON_0001088 a rdfs:Class ;
  rdfs:label "urine" ;
  rdfs:comment "Excretion that is the output of a kidney" ;
  rdfs:subClassOf obo:UBERON_0000463 .

obo:ECO_0007512 a rdfs:Class ;
  rdfs:label "beta-glucuronidase reporter gene assay evidence used in automatic assertion" ;
  rdfs:comment "A type of beta-glucuronidase reporter gene assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001804 , obo:ECO_0007765 .

obo:ECO_0007404 a rdfs:Class ;
  rdfs:label "in vitro polyADP-ribosylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro polyADP-ribosylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001069 , obo:ECO_0007797 .

obo:ECO_0007753 a rdfs:Class ;
  rdfs:label "curator inference from encyclopedia used in manual assertion" ;
  rdfs:comment "A type of curator inference from encyclopedia that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007637 , obo:ECO_0007752 .

obo:ECO_0000349 a rdfs:Class ;
  rdfs:label "full support of intron positions by RNA-sequencing alignment evidence used in manual assertion" ;
  rdfs:comment "A type of full support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000343 , obo:ECO_0000346 .

obo:OBI_0002447 a rdfs:Class ;
  rdfs:label "suppression subtractive hybridization" ;
  rdfs:comment "An artificially induced nucleic acid hybridization that is performed to compare gene expression in different cell or tissue types based on normalization and suppression, which creates a subtracted cDNA or genomic DNA library." ;
  rdfs:subClassOf obo:OBI_0000253 .

obo:ECO_0007462 a rdfs:Class ;
  rdfs:label "urine test evidence used in automatic assertion" ;
  rdfs:comment "A type of urine test evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001134 , obo:ECO_0007307 .

obo:ECO_0007805 a rdfs:Class ;
  rdfs:label "substance quantification evidence used in automatic assertion" ;
  rdfs:comment "A type of substance quantification evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000335 , obo:ECO_0007307 .

obo:ECO_0000005 a rdfs:Class ;
  rdfs:label "enzymatic activity assay evidence" ;
  rdfs:comment "A type of protein assay evidence where the catalytic activity of an enzyme is determined." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0008158 a rdfs:Class ;
  rdfs:label "antibody Tu39-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody Tu39-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008074 , obo:ECO_0008088 .

obo:ECO_0006397 a rdfs:Class ;
  rdfs:label "self-reported ancestry evidence used in automatic assertion" ;
  rdfs:comment "A type of self-reported ancestry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006158 , obo:ECO_0006395 .

obo:ECO_0007745 a rdfs:Class ;
  rdfs:label "combinatorial experimental evidence used in manual assertion" ;
  rdfs:comment "A type of combinatorial experimental evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000244 , obo:ECO_0007654 .

obo:GO_0009894 a rdfs:Class ;
  rdfs:label "regulation of catabolic process" ;
  rdfs:subClassOf obo:GO_0019222 , obo:GO_0065007 .

obo:UBERON_0000955 a rdfs:Class ;
  rdfs:label "brain" ;
  rdfs:comment "The brain is the center of the nervous system in all vertebrate, and most invertebrate, animals. Some primitive animals such as jellyfish and starfish have a decentralized nervous system without a brain, while sponges lack any nervous system at all. In vertebrates, the brain is located in the head, protected by the skull and close to the primary sensory apparatus of vision, hearing, balance, taste, and smell[WP]." ;
  rdfs:subClassOf obo:UBERON_0000465 .

obo:ECO_0000303 a rdfs:Class ;
  rdfs:label "author statement without traceable support used in manual assertion" ;
  rdfs:comment "A type of author statement without traceable support that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/nas-non-traceable-author-statement> ;
  rdfs:subClassOf obo:ECO_0000034 , obo:ECO_0000302 .

obo:ECO_0007569 a rdfs:Class ;
  rdfs:label "split-ubiquitin functional complementation evidence used in automatic assertion" ;
  rdfs:comment "A type of split-ubiquitin functional complementation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000074 , obo:ECO_0006294 .

obo:ECO_0000077 a rdfs:Class ;
  rdfs:label "methylation-specific polymerase chain reaction evidence" ;
  rdfs:comment "A type of in vitro methylation assay evidence resulting from initial modification of DNA by sodium bisulfite, converting all unmethylated cytosines to uracil, and subsequent amplification with primers specific for methylated versus unmethylated DNA." ;
  rdfs:subClassOf obo:ECO_0001065 .

obo:ECO_0001070 a rdfs:Class ;
  rdfs:label "in vitro sumoylation assay evidence" ;
  rdfs:comment "A type of sumoylation assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001559 .

obo:ECO_0007697 a rdfs:Class ;
  rdfs:label "DNA synthesis cell proliferation assay evidence used in manual assertion" ;
  rdfs:comment "A type of DNA synthesis cell proliferation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005008 , obo:ECO_0007696 .

obo:OBI_0001630 a rdfs:Class ;
  rdfs:label "analytical chromatography" ;
  rdfs:comment "An analyte assay that uses a biomaterial's preferential affinity for either the mobile phase or the stationary phase to separate it from other materials and thereby detect its presence in an input material." ;
  rdfs:subClassOf obo:OBI_0000443 .

obo:GO_0016049 a rdfs:Class ;
  rdfs:label "cell growth" ;
  rdfs:subClassOf obo:GO_0009987 , obo:GO_0040007 .

obo:GO_1900139 a rdfs:Class ;
  rdfs:label "negative regulation of arachidonic acid secretion" ;
  rdfs:subClassOf obo:GO_0032304 , obo:GO_0065007 , obo:GO_0090237 .

obo:ECO_0007545 a rdfs:Class ;
  rdfs:label "Kadouri drip-fed biofilm assay evidence used in automatic assertion" ;
  rdfs:comment "A type of Kadouri drip-fed biofilm assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006028 , obo:ECO_0007541 .

obo:GO_1905953 a rdfs:Class ;
  rdfs:label "negative regulation of lipid localization" ;
  rdfs:subClassOf obo:GO_0048519 , obo:GO_0065007 , obo:GO_1905952 .

obo:ECO_0000023 a rdfs:Class ;
  rdfs:label "affinity evidence" ;
  rdfs:comment "A type of physical interaction evidence that depends on the strength of the interaction between two entities." ;
  rdfs:subClassOf obo:ECO_0000021 .

obo:GO_0051253 a rdfs:Class ;
  rdfs:label "negative regulation of RNA metabolic process" ;
  rdfs:subClassOf obo:GO_0010605 , obo:GO_0045934 , obo:GO_0051252 , obo:GO_0065007 .

obo:ECO_0005031 a rdfs:Class ;
  rdfs:label "structure determination evidence" ;
  rdfs:comment "A type of experimental evidence resulting from the visualization and examination of chemical and/or molecular structures." ;
  rdfs:subClassOf obo:BFO_0000002 , obo:ECO_0000006 .

obo:ECO_0008147 a rdfs:Class ;
  rdfs:label "antibody MKD6-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody MKD6-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008069 , obo:ECO_0008087 .

obo:ECO_0007142 a rdfs:Class ;
  rdfs:label "green fluorescent protein immunolocalization evidence used in manual assertion" ;
  rdfs:comment "A type of green fluorescent protein immunolocalization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000300 , obo:ECO_0006031 .

obo:ECO_0001255 a rdfs:Class ;
  rdfs:label "radioimmunoassay evidence used in manual assertion" ;
  rdfs:comment "A type of radioimmunoassay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001121 .

obo:ECO_0001813 a rdfs:Class ;
  rdfs:label "heteronuclear single quantum coherence spectroscopy evidence" ;
  rdfs:comment "A type of nuclear magnetic resonance spectroscopy evidence based on two-dimensional NMR for elucidation of the chemical structure of an isolated or synthesized chemical compound where the characteristic transfer magnetization of a proton to a nitrogen or carbon isotope is monitored by NMR, generating a specific peak in the spectrum." ;
  rdfs:subClassOf obo:ECO_0006163 .

obo:ECO_0006230 a rdfs:Class ;
  rdfs:label "heat capacity-based evidence used in manual assertion" ;
  rdfs:comment "A type of heat capacity-based evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0006192 .

obo:ECO_0001165 a rdfs:Class ;
  rdfs:label "colony counting evidence used in manual assertion" ;
  rdfs:comment "A type of colony counting evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001027 .

obo:ECO_0005513 a rdfs:Class ;
  rdfs:label "repeat expansion mutation phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence resulting from a mutation that increases the number of times that a DNA sequence is repeated." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0006301 a rdfs:Class ;
  rdfs:label "protein thermal shift assay evidence used in manual assertion" ;
  rdfs:comment "A type of protein thermal shift assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006260 , obo:ECO_0006317 .

obo:ECO_0007422 a rdfs:Class ;
  rdfs:label "in vivo sumoylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo sumoylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001086 , obo:ECO_0007788 .

obo:ECO_0000088 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence" ;
  rdfs:comment "A type of curator inference that is based on a combination of experimental evidences or existing models of that system in a related species used for the reconstruction of a biological system." ;
  rdfs:subClassOf obo:ECO_0000205 .

obo:ECO_0007291 a rdfs:Class ;
  rdfs:label "tumble frequency assay evidence used in automatic assertion" ;
  rdfs:comment "A type of tumble frequency assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005608 , obo:ECO_0007780 .

obo:ECO_0006021 a rdfs:Class ;
  rdfs:label "biofilm formation assay evidence used in manual assertion" ;
  rdfs:comment "A type of biofilm formation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006020 , obo:ECO_0007696 .

obo:ECO_0007064 a rdfs:Class ;
  rdfs:label "dynamic light scattering assay evidence used in manual assertion" ;
  rdfs:comment "A type of dynamic light scattering assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007062 , obo:ECO_0007063 .

obo:ECO_0007799 a rdfs:Class ;
  rdfs:label "translation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of translation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001561 , obo:ECO_0007307 .

obo:ECO_0006100 a rdfs:Class ;
  rdfs:label "recessive mutant phenotype evidence" ;
  rdfs:comment "A type of mutant phenotype evidence based on the analysis of a mutation which, in diploid organisms, must be present in both alleles for a phenotype to manifest itself." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0001209 a rdfs:Class ;
  rdfs:label "in vivo deacetylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo deacetylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001076 .

obo:GO_0009607 a rdfs:Class ;
  rdfs:label "response to biotic stimulus" ;
  rdfs:subClassOf obo:GO_0050896 .

obo:OBI_1110021 a rdfs:Class ;
  rdfs:label "infection process"@en ;
  rdfs:comment "the detrimental process in which an infectious agent colonizes or replicates in a host environment"@en ;
  rdfs:subClassOf obo:OBI_1110122 .

obo:OBI_0001305 a rdfs:Class ;
  rdfs:label "genotype information"@en ;
  rdfs:comment "a genetic characteristics information that is about the genetic material of an organism and minimally includes information about the genetic background and can in addition contain information about specific alleles, genetic modifications, etc."@en ;
  rdfs:subClassOf obo:OBI_0001404 .

obo:ECO_0007005 a rdfs:Class ;
  rdfs:label "high throughput direct assay evidence used in manual assertion" ;
  rdfs:comment "A type of high throughput evidence that is used in a manual assertion derived from the high throughput direct measurement of some aspect of a biological feature." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0006056 , obo:ECO_0007004 .

obo:ECO_0005576 a rdfs:Class ;
  rdfs:label "voltage clamp recording evidence" ;
  rdfs:comment "A type of electrophysiology assay evidence that involves recording membrane potential by controlling the membrane voltage and measuring the transmembrane current required to maintain that voltage." ;
  rdfs:subClassOf obo:ECO_0000164 .

obo:ECO_0001250 a rdfs:Class ;
  rdfs:label "protein dot blot assay evidence used in manual assertion" ;
  rdfs:comment "A type of protein dot blot assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001116 .

obo:OBI_0001919 a rdfs:Class ;
  rdfs:label "Carbon-copy chromosome conformation capture assay" ;
  rdfs:comment "An assay that measures the organization of chromosomes at the genome-wide scale." ;
  rdfs:subClassOf obo:OBI_0000626 , obo:OBI_0002692 .

obo:ECO_0001802 a rdfs:Class ;
  rdfs:label "beta-galactosidase reporter gene assay evidence" ;
  rdfs:comment "A type of reporter gene assay evidence based on the fusion of the lacZ gene to a specific promoter for the expression of beta-galactosidase which will appear blue when grown on a X-gal medium." ;
  rdfs:subClassOf obo:ECO_0000049 .

obo:GO_0043069 a rdfs:Class ;
  rdfs:label "negative regulation of programmed cell death" ;
  rdfs:subClassOf obo:GO_0043067 , obo:GO_0060548 , obo:GO_0065007 .

obo:ECO_0001093 a rdfs:Class ;
  rdfs:label "luminescence-based mammalian interactome mapping assay evidence" ;
  rdfs:comment "A type of physical interaction evidence resulting from the analysis of protein-protein interactions by use of a luciferase enzyme fused to a particular protein (or proteins), which are coexpressed with epitope-tagged partners in mammalian cells." ;
  rdfs:subClassOf obo:ECO_0000021 .

obo:GO_0032102 a rdfs:Class ;
  rdfs:label "negative regulation of response to external stimulus" ;
  rdfs:subClassOf obo:GO_0032101 , obo:GO_0048585 , obo:GO_0065007 .

obo:CL_0000010 a rdfs:Class ;
  rdfs:label "cultured cell" ;
  rdfs:comment "A cell in vitro that is or has been maintained or propagated as part of a cell culture." ;
  rdfs:subClassOf obo:CL_0000578 .

obo:GO_0040030 a rdfs:Class ;
  rdfs:label "regulation of molecular function, epigenetic" ;
  rdfs:comment "Any heritable epigenetic process that modulates the frequency, rate or extent of protein function by self-perpetuating conformational conversions of normal proteins in healthy cells. This is distinct from, though mechanistically analogous to, disease states associated with prion propagation and amyloidogenesis. A single protein, if it carries a glutamine/asparagine-rich ('prion') domain, can sometimes stably exist in at least two distinct physical states, each associated with a different phenotype; propagation of one of these traits is achieved by a self-perpetuating change in the protein from one form to the other, mediated by conformational changes in the glutamine/asparagine-rich domain. Prion domains are both modular and transferable to other proteins, on which they can confer a heritable epigenetic alteration of function; existing bioinformatics data indicate that they are rare in non-eukarya, but common in eukarya." ;
  rdfs:subClassOf obo:GO_0008150 .

obo:ECO_0000343 a rdfs:Class ;
  rdfs:label "full support of intron positions by RNA-sequencing alignment evidence" ;
  rdfs:comment "A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignment from a single sample supports all of the intron positions (exon pairs) predicted for a transcript." ;
  rdfs:subClassOf obo:ECO_0000342 .

obo:ECO_0006381 a rdfs:Class ;
  rdfs:label "asexual sporulation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of asexual sporulation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006345 , obo:ECO_0006379 .

obo:ECO_0007649 a rdfs:Class ;
  rdfs:label "manually integrated combinatorial computational evidence used in manual assertion" ;
  rdfs:comment "A type of manually integrated combinatorial computational evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007648 , obo:ECO_0007675 , obo:ECO_0007678 .

obo:GO_0043269 a rdfs:Class ;
  rdfs:label "regulation of ion transport" ;
  rdfs:subClassOf obo:GO_0051049 , obo:GO_0065007 .

obo:OBI_0001285 a rdfs:Class ;
  rdfs:label "in vitro design" ;
  rdfs:comment "A study design that is done in a test tube or a culture dish, e.g. A bacterial invasion assay in an established cell culture." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:ECO_0005528 a rdfs:Class ;
  rdfs:label "site-directed mutagenesis phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype phenotype evidence based on target-specified mutagenesis for the characterization of various gene and protein interactions, structures, and functions where oligonucleotide primers are used to alter a nucleotide sequence." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0007842 a rdfs:Class ;
  rdfs:label "curator inference from Oxford Dictionary used in automatic assertion" ;
  rdfs:comment "A type of curator inference from Oxford Dictionary that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007642 , obo:ECO_0007839 .

obo:GO_0048519 a rdfs:Class ;
  rdfs:label "negative regulation of biological process" ;
  rdfs:subClassOf obo:GO_0050789 , obo:GO_0065007 .

obo:ECO_0007410 a rdfs:Class ;
  rdfs:label "in vivo acetylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo acetylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001074 , obo:ECO_0007794 .

obo:ECO_0008001 a rdfs:Class ;
  rdfs:label "deep learning neural network method evidence" ;
  rdfs:comment "A type of neural network method evidence which leverages a broad suite of neural network models, which use a combination of data attributes of an entity called features as inputs, and map them to outputs through multiple layers of connected hidden units, whose parameters are learned from data." ;
  rdfs:subClassOf obo:ECO_0008013 .

obo:ECO_0007238 a rdfs:Class ;
  rdfs:label "Hi-C evidence used in automatic assertion" ;
  rdfs:comment "A type of Hi-C evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000238 , obo:ECO_0007822 .

obo:ECO_0001087 a rdfs:Class ;
  rdfs:label "in vivo transcription assay evidence" ;
  rdfs:comment "A type of transcription assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0000168 .

obo:GO_0044085 a rdfs:Class ;
  rdfs:label "cellular component biogenesis" ;
  rdfs:subClassOf obo:GO_0071840 .

obo:ECO_0007617 a rdfs:Class ;
  rdfs:label "light scattering evidence used in automatic assertion" ;
  rdfs:comment "A type of light scattering evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007061 , obo:ECO_0007815 .

obo:IAO_0000308 a rdfs:Class ;
  rdfs:label "figure"@en ;
  rdfs:comment "An information content entity consisting of a two dimensional arrangement of information content entities such that the arrangement itself is about something."@en ;
  rdfs:subClassOf obo:IAO_0000030 .

obo:ECO_0000287 a rdfs:Class ;
  rdfs:label "differential hybridization evidence used in manual assertion" ;
  rdfs:comment "A type of differential hybridization experiment that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000116 , obo:ECO_0000291 .

obo:ECO_0007079 a rdfs:Class ;
  rdfs:label "colony color phenotypic evidence" ;
  rdfs:comment "A type of colony morphology evidence resulting from studying the colony pigmentation." ;
  rdfs:subClassOf obo:ECO_0007077 .

obo:ECO_0001050 a rdfs:Class ;
  rdfs:label "histochemistry evidence" ;
  rdfs:comment "A type of histology evidence resulting from the identification of chemical components in cells and tissues." ;
  rdfs:subClassOf obo:ECO_0001051 .

obo:ECO_0006189 a rdfs:Class ;
  rdfs:label "cryogenic electron microscopy-based structural model with missing residue coordinates" ;
  rdfs:comment "A type of cryogenic electron microscopy evidence in which a structural model is built lacking coordinates for some residues indicating high flexibility or structural disorder in that area of the molecule." ;
  rdfs:subClassOf obo:ECO_0006181 .

obo:ECO_0000247 a rdfs:Class ;
  rdfs:label "sequence alignment evidence used in manual assertion" ;
  rdfs:comment "A type of sequence alignment evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/isa-inferred-sequence-alignment> ;
  rdfs:subClassOf obo:ECO_0000200 , obo:ECO_0000250 .

obo:ECO_0000316 a rdfs:Class ;
  rdfs:label "genetic interaction evidence used in manual assertion" ;
  rdfs:comment "A type of genetic interaction experiment evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/igi-inferred-genetic-interaction> ;
  rdfs:subClassOf obo:ECO_0000011 , obo:ECO_0007634 .

obo:ECO_0007490 a rdfs:Class ;
  rdfs:label "confocal microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of confocal microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005587 , obo:ECO_0007435 .

obo:ECO_0007012 a rdfs:Class ;
  rdfs:label "combinatorial experimental and curator inference evidence" ;
  rdfs:comment "A type of combinatorial evidence in which the curator draws a conclusion based on a combination of prior scientific background knowledge and evidence from one or more experiments." ;
  rdfs:subClassOf obo:ECO_0000212 .

obo:ECO_0001273 a rdfs:Class ;
  rdfs:label "ectopic expression evidence used in manual assertion" ;
  rdfs:comment "A type of ectopic expression evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000017 , obo:ECO_0007634 .

obo:GO_0120031 a rdfs:Class ;
  rdfs:label "plasma membrane bounded cell projection assembly" ;
  rdfs:subClassOf obo:GO_0030031 , obo:GO_0120036 .

obo:GO_0030335 a rdfs:Class ;
  rdfs:label "positive regulation of cell migration" ;
  rdfs:subClassOf obo:GO_0030334 , obo:GO_0065007 , obo:GO_2000147 .

obo:GO_0010467 a rdfs:Class ;
  rdfs:label "gene expression" ;
  rdfs:comment "The process in which a gene's sequence is converted into a mature gene product or products (proteins or RNA). This includes the production of an RNA transcript as well as any processing to produce a mature RNA product or an mRNA or circRNA (for protein-coding genes) and the translation of that mRNA or circRNA into protein. Protein maturation is included when required to form an active form of a product from an inactive precursor form." ;
  rdfs:subClassOf obo:GO_0043170 .

obo:ECO_0007725 a rdfs:Class ;
  rdfs:label "protein localization evidence used in manual assertion" ;
  rdfs:comment "A type of protein localization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000122 , obo:ECO_0000314 .

obo:ECO_0001154 a rdfs:Class ;
  rdfs:label "Boyden chamber assay evidence used in manual assertion" ;
  rdfs:comment "A type of Boyden chamber assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001017 , obo:ECO_0007701 .

obo:ECO_0008095 a rdfs:Class ;
  rdfs:label "antibody 34-1-2S-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody 34-1-2S-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008043 , obo:ECO_0008087 .

obo:GO_0003677 a rdfs:Class ;
  rdfs:label "DNA binding" ;
  rdfs:subClassOf obo:GO_0003676 .

obo:ECO_0001244 a rdfs:Class ;
  rdfs:label "peptide affinity enrichment evidence used in manual assertion" ;
  rdfs:comment "A type of peptide affinity enrichment evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001110 , obo:ECO_0006075 .

obo:ECO_0007682 a rdfs:Class ;
  rdfs:label "reporter gene assay evidence used in manual assertion" ;
  rdfs:comment "A type of reporter gene assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000049 , obo:ECO_0000270 .

obo:GO_0016787 a rdfs:Class ;
  rdfs:label "hydrolase activity" ;
  rdfs:subClassOf obo:GO_0003824 .

obo:BFO_0000040 a rdfs:Class ;
  rdfs:label "material entity"@en ;
  rdfs:comment "An independent continuant that is spatially extended whose identity is independent of that of other entities and can be maintained through time."@en ;
  rdfs:subClassOf obo:BFO_0000004 .

obo:OBI_0400168 a rdfs:Class ;
  rdfs:label "microtome" ;
  rdfs:comment "A microtome is a mechanical instrument used to cut biological specimens into very thin segments for further treatment (e.g. ISH) and ultimately microscopic or histologic examination. Most microtomes provide cooling facilities (cryo-microtome) and use a steel blade to cut a slice of defined thickness. Some are automatic, and some are driven by hand." ;
  rdfs:subClassOf obo:OBI_0000932 .

obo:OBI_0001936 a rdfs:Class ;
  rdfs:label "molecular-labeled material" ;
  rdfs:comment "a material entity that is the specified output of an addition of molecular label process that aims to label some molecular target to allow for its detection in a detection of molecular label assay" ;
  rdfs:subClassOf obo:BFO_0000040 , obo:OBI_0000047 .

obo:ECO_0007630 a rdfs:Class ;
  rdfs:label "ribosome profiling evidence used in automatic assertion" ;
  rdfs:comment "A type of ribosome profiling evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007087 , obo:ECO_0007298 .

obo:ECO_0007190 a rdfs:Class ;
  rdfs:label "obsolete inference from phenotype manipulation evidence used in manual assertion" ;
  rdfs:comment "OBSOLETE. A type of inference from phenotype manipulation evidence that is used in a manual assertion." .

obo:ECO_0007794 a rdfs:Class ;
  rdfs:label "acetylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of acetylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001546 , obo:ECO_0007307 .

obo:GO_0008289 a rdfs:Class ;
  rdfs:label "lipid binding" ;
  rdfs:subClassOf obo:GO_0005488 .

obo:ECO_0007221 a rdfs:Class ;
  rdfs:label "mutant physiological response evidence used in automatic assertion" ;
  rdfs:comment "A type of mutant physiological response evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000174 , obo:ECO_0007325 .

obo:ECO_0007646 a rdfs:Class ;
  rdfs:label "curator inference from book" ;
  rdfs:comment "A type of curator inference from published work based on a book, which may be a reference to a URL (for ebooks) or a DOI." ;
  rdfs:subClassOf obo:ECO_0007647 .

obo:ECO_0007822 a rdfs:Class ;
  rdfs:label "chromosome conformation-based evidence used in automatic assertion" ;
  rdfs:comment "A type of chromosome conformation-based evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000232 , obo:ECO_0007297 .

obo:ECO_0008016 a rdfs:Class ;
  rdfs:label "single molecule fluorescence in situ hybridization evidence used in manual assertion" ;
  rdfs:comment "A type of single molecule fluorescence in situ hybridization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001182 , obo:ECO_0007739 , obo:ECO_0008009 .

obo:ECO_0007150 a rdfs:Class ;
  rdfs:label "plasmid maintenance assay evidence used in manual assertion" ;
  rdfs:comment "A type of plasmid maintenance assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0000340 .

obo:ECO_0007288 a rdfs:Class ;
  rdfs:label "protein mass spectrometry evidence used in automatic assertion" ;
  rdfs:comment "A type of protein mass spectrometry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005603 , obo:ECO_0007433 .

obo:GO_0071897 a rdfs:Class ;
  rdfs:label "DNA biosynthetic process" ;
  rdfs:subClassOf obo:GO_0006259 , obo:GO_0034645 , obo:GO_0034654 .

obo:ECO_0007540 a rdfs:Class ;
  rdfs:label "inference based on individual clinical experience used in automatic assertion" ;
  rdfs:comment "A type of inference based on individual clinical experience that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006018 , obo:ECO_0007322 .

obo:ECO_0001098 a rdfs:Class ;
  rdfs:label "microscopy evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from the use of a microscope." ;
  rdfs:subClassOf obo:ECO_0000324 .

obo:OBI_0003123 a rdfs:Class ;
  rdfs:label "3D neural cell structure determination assay" ;
  rdfs:comment "A 3D cell structure determination assay that determines the 3-dimensional configuration of a neural cell." ;
  rdfs:subClassOf obo:OBI_0003122 .

obo:ECO_0006205 a rdfs:Class ;
  rdfs:label "far-UV circular dichroism evidence used in automatic assertion" ;
  rdfs:comment "A type of far-UV circular dichroism evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006179 , obo:ECO_0006201 .

obo:ECO_0008119 a rdfs:Class ;
  rdfs:label "antibody CR11-351-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody CR11-351-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008055 , obo:ECO_0008087 .

obo:OBI_0001852 a rdfs:Class ;
  rdfs:label "paired-end library preparation"@en ;
  rdfs:comment "A library preparation that results in the creation of a library of the 5' and 3' ends of DNA or cDNA fragments using adaptors and endonucleases. The preparation may or may not include cloning process. " ;
  rdfs:subClassOf obo:OBI_0000711 .

obo:OBI_0000070 a rdfs:Class ;
  rdfs:label "assay"@en ;
  rdfs:comment "A planned process with the objective to produce information about the material entity that is the evaluant, by physically examining it or its proxies."@en ;
  rdfs:subClassOf obo:OBI_0000011 .

obo:ECO_0007006 a rdfs:Class ;
  rdfs:label "high throughput expression pattern evidence" ;
  rdfs:comment "A type of high throughput evidence derived from the high throughput characterization of gene expression." ;
  rdfs:subClassOf obo:ECO_0000008 , obo:ECO_0006055 .

obo:ECO_0001248 a rdfs:Class ;
  rdfs:label "propidium iodide staining evidence used in manual assertion" ;
  rdfs:comment "A type of propidium iodide staining evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001114 .

obo:ECO_0001807 a rdfs:Class ;
  rdfs:label "electrophoretic mobility shift assay evidence used in manual assertion" ;
  rdfs:comment "A type of electrophoretic mobility shift that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000096 , obo:ECO_0000353 .

obo:GO_0006518 a rdfs:Class ;
  rdfs:label "peptide metabolic process" ;
  rdfs:subClassOf obo:GO_0043603 , obo:GO_1901564 .

obo:ECO_0000237 a rdfs:Class ;
  rdfs:label "3C-qPCR evidence" ;
  rdfs:comment "A type of 3C evidence evidence that 1) quantifies interactions between a single pair of genomic loci (not multiple interactions) and 2) performs quantitative polymerase chain reaction (qPCR) in the quantitation step." ;
  rdfs:subClassOf obo:ECO_0000233 .

obo:GO_0048585 a rdfs:Class ;
  rdfs:label "negative regulation of response to stimulus" ;
  rdfs:subClassOf obo:GO_0048519 , obo:GO_0048583 , obo:GO_0065007 .

obo:ECO_0007551 a rdfs:Class ;
  rdfs:label "genetically encoded fluorescent ion concentration sensor assay evidence used in automatic assertion" ;
  rdfs:comment "A type of genetically encoded fluorescent ion concentration sensor assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006040 , obo:ECO_0007550 .

obo:ECO_0000265 a rdfs:Class ;
  rdfs:label "sequence orthology evidence used in automatic assertion" ;
  rdfs:comment "A type of sequence orthology evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000201 , obo:ECO_0000249 .

obo:OBI_0000275 a rdfs:Class ;
  rdfs:label "analyte role"@en ;
  rdfs:comment "A measurand role borne by a molecular entity or an atom and realized in an analyte assay which achieves the objective to measure the magnitude/concentration/amount of the analyte in the entity bearing evaluant role."@en ;
  rdfs:subClassOf obo:OBI_0002444 .

obo:RO_0000081 a rdf:Property ;
  rdfs:label "role of"@en ;
  rdfs:comment "a relation between a role and an independent continuant (the bearer), in which the role specifically depends on the bearer for its existence"@en ;
  rdfs:range <https://w3id.org/linkml/Any> ;
  rdfs:subPropertyOf obo:RO_0000052 .

obo:ECO_0007402 a rdfs:Class ;
  rdfs:label "in vitro palmitoylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro palmitoylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001066 , obo:ECO_0007792 .

obo:ECO_0006026 a rdfs:Class ;
  rdfs:label "colony biofilm assay evidence" ;
  rdfs:comment "A type of biofilm formation assay evidence in which a colony is grown on a semipermeable membrane on an agar plate. The plate serves to supply nutrients and the semipermeable membrane can be relocated to fresh plates." ;
  rdfs:subClassOf obo:ECO_0006020 .

obo:ECO_0000054 a rdfs:Class ;
  rdfs:label "double mutant phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence resulting from an experiment typically constructed to determine if two different genes have an observable genetic interaction (functional connection) as the result of a mutation occurring in the alleles of the two genes of interest." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0000050 a rdfs:Class ;
  rdfs:label "voucher specimen phenotypic analysis evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence based on an authors phenotypic description of a species (or higher-level group), which explicitly references an observation made of a voucher specimen (a specimen with a permanent museum catalog)." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:GO_0051270 a rdfs:Class ;
  rdfs:label "regulation of cellular component movement" ;
  rdfs:subClassOf obo:GO_0032879 , obo:GO_0050794 , obo:GO_0065007 .

obo:GO_0065009 a rdfs:Class ;
  rdfs:label "regulation of molecular function" ;
  rdfs:subClassOf obo:GO_0065007 .

obo:ECO_0005621 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation-seq evidence used in automatic assertion" ;
  rdfs:comment "A type of chromatin immunoprecipitation-seq evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000229 , obo:ECO_0007786 .

obo:ECO_0005611 a rdfs:Class ;
  rdfs:label "obsolete inference from experimental data evidence" ;
  rdfs:comment "OBSOLETE. A type of direct assay evidence used in manual assertion where phenotype or disease association is made with the gene because of involvement in the molecular mechanism of disease or involvement in a pathway known to contribute to a disease." .

obo:ECO_0001826 a rdfs:Class ;
  rdfs:label "ultraviolet light footprinting evidence" ;
  rdfs:comment "A type of nucleic acid binding evidence where DNA is non-specifically fragmented with ultraviolet light while protein-bound regions are protected from UV damage and strand breakage patterns are analyzed by PAGE and sequenced to detect protein-DNA contacts." ;
  rdfs:subClassOf obo:ECO_0000136 .

obo:GO_0032070 a rdfs:Class ;
  rdfs:label "regulation of deoxyribonuclease activity" ;
  rdfs:subClassOf obo:GO_0032069 , obo:GO_0051052 , obo:GO_0065007 .

obo:OBI_0400116 a rdfs:Class ;
  rdfs:label "thermal cycler" ;
  rdfs:comment "An instrument that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time." ;
  rdfs:subClassOf obo:OBI_0002089 .

obo:GO_0022857 a rdfs:Class ;
  rdfs:label "transmembrane transporter activity" ;
  rdfs:subClassOf obo:GO_0005215 .

obo:ECO_0005601 a rdfs:Class ;
  rdfs:label "immunofluorescence confocal microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of immunofluorescence confocal microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005589 , obo:ECO_0005600 , obo:ECO_0005804 .

obo:ECO_0001577 a rdfs:Class ;
  rdfs:label "spheroplast fusion assay evidence" ;
  rdfs:comment "A type of membrane fusion assay evidence based on the fusion of spheroplasts." ;
  rdfs:subClassOf obo:ECO_0001574 .

obo:OBI_0000973 a rdfs:Class ;
  rdfs:label "sequence data"@en ;
  rdfs:comment """A measurement datum that representing the primary structure of a macromolecule(it's sequence) sometimes associated with an indicator of confidence of that measurement.
""" .

obo:ECO_0000243 a rdfs:Class ;
  rdfs:label "tissue grafting phenotypic evidence" ;
  rdfs:comment "A type of anatomical perturbation phenotypic evidence resulting from the addition of tissue to an organism through a graft procedure." ;
  rdfs:subClassOf obo:ECO_0000240 .

obo:ECO_0006404 a rdfs:Class ;
  rdfs:label "experimentally evolved mutant phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence where mutations arising in organisms/cells over multiple generations, with or without exposure to some kind of selective pressure, are monitored or assayed." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0007087 a rdfs:Class ;
  rdfs:label "ribosome profiling evidence" ;
  rdfs:comment "A type of expression pattern evidence in which translation is measured by the positions of ribosomes active in a cell, identified through deep sequencing of ribosome-protected mRNA fragments." ;
  rdfs:subClassOf obo:ECO_0000008 .

obo:OBI_0000932 a rdfs:Class ;
  rdfs:label "material separation device" ;
  rdfs:comment "A device with a separation function realized in a planed process" ;
  rdfs:subClassOf obo:OBI_0000968 .

obo:ECO_0000250 a rdfs:Class ;
  rdfs:label "sequence similarity evidence used in manual assertion" ;
  rdfs:comment "A type of sequence similarity evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/iss-inferred-sequence-or-structural-similarity> ;
  rdfs:subClassOf obo:ECO_0000044 , obo:ECO_0000252 , obo:ECO_0007668 .

obo:ECO_0006170 a rdfs:Class ;
  rdfs:label "quantitative western immunoblotting evidence used in manual assertion" ;
  rdfs:comment "A type of quantitative western immunoblotting evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000279 , obo:ECO_0006169 .

obo:GO_0032055 a rdfs:Class ;
  rdfs:label "negative regulation of translation in response to stress" ;
  rdfs:subClassOf obo:GO_0017148 , obo:GO_0043555 , obo:GO_0065007 .

obo:ECO_0007032 a rdfs:Class ;
  rdfs:label "compement fixation assay evidence used in manual assertion" ;
  rdfs:comment "A type of compement fixation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006078 , obo:ECO_0007031 .

obo:ECO_0008108 a rdfs:Class ;
  rdfs:label "antibody B22-249.R1-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody B22-249.R1-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008049 , obo:ECO_0008088 .

obo:ECO_0000176 a rdfs:Class ;
  rdfs:label "mutant visible phenotype evidence" ;
  rdfs:comment "A type of mutant phenotype evidence in which traits arising from a mutation are visually examined." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0007097 a rdfs:Class ;
  rdfs:label "developmental similarity evidence used in manual assertion" ;
  rdfs:comment "A type of developmental similarity evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000067 , obo:ECO_0000252 .

obo:ECO_0007734 a rdfs:Class ;
  rdfs:label "high throughput nucleotide sequencing assay evidence used in manual assertion" ;
  rdfs:comment "A type of high throughput nucleotide sequencing assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000221 , obo:ECO_0000269 .

obo:GO_0032092 a rdfs:Class ;
  rdfs:label "positive regulation of protein binding" ;
  rdfs:subClassOf obo:GO_0043393 , obo:GO_0051099 , obo:GO_0065007 .

obo:ECO_0007840 a rdfs:Class ;
  rdfs:label "curator inference from MedlinePlus dictionary used in automatic assertion" ;
  rdfs:comment "A type of curator inference from MedlinePlus dictionary that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007644 , obo:ECO_0007839 .

obo:ECO_0000334 a rdfs:Class ;
  rdfs:label "particle size and count assay evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from an assay where both the size of and number of particles in a sample are determined." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007047 a rdfs:Class ;
  rdfs:label "time-lapsed microscopy evidence" ;
  rdfs:comment "A type of microscopy evidence where a serial images are taken at regular time points to capture the dynamics of what is being observed." ;
  rdfs:subClassOf obo:ECO_0001098 .

obo:OBI_0600043 a rdfs:Class ;
  rdfs:label "genetic transformation"@en ;
  rdfs:comment "the introduction. alteration or integration of genetic material into a cell or organism"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0007366 a rdfs:Class ;
  rdfs:label "comet assay evidence used in automatic assertion" ;
  rdfs:comment "A type of comet assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001029 , obo:ECO_0007769 .

obo:ECO_0007247 a rdfs:Class ;
  rdfs:label "beta-galactosidase protein immunolocalization evidence used in automatic assertion" ;
  rdfs:comment "A type of beta-galactosidase protein immunolocalization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000301 , obo:ECO_0007486 .

obo:ECO_0007566 a rdfs:Class ;
  rdfs:label "immunofluorescence super resolution microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of immunofluorescence super resolution microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006072 , obo:ECO_0007533 .

obo:ECO_0005589 a rdfs:Class ;
  rdfs:label "confocal microscopy evidence used in manual assertion" ;
  rdfs:comment "A type confocal microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001232 , obo:ECO_0005587 .

obo:ECO_0007599 a rdfs:Class ;
  rdfs:label "indirect Coombs test evidence used in automatic assertion" ;
  rdfs:comment "A type of indirect Coombs test evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007025 , obo:ECO_0007596 .

obo:GO_0001817 a rdfs:Class ;
  rdfs:label "regulation of cytokine production" ;
  rdfs:subClassOf obo:GO_0010468 , obo:GO_0051239 , obo:GO_0065007 .

obo:OBI_0302890 a rdfs:Class ;
  rdfs:label "polymerization"@en ;
  rdfs:comment "polymerization is process by which molecular entity of small mass are aggregated in motifs over the course of a chemical reaction catalyzed by enzymes or other molecular entities acting as catalyst. polymerization results in molecular entity of high molecular weight"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0000215 a rdfs:Class ;
  rdfs:label "rapid divergence from ancestral sequence evidence" ;
  rdfs:comment "A type of phylogenetic evidence characterized by long phylogenetic tree branch lengths following a duplication event." ;
  rdfs:subClassOf obo:ECO_0000080 .

obo:ECO_0006084 a rdfs:Class ;
  rdfs:label "ribohomopolymer binding assay evidence used in manual assertion" ;
  rdfs:comment "A type of nucleic acid binding evidence that is used in a manual assertion resulting from an enzyme displaying binding activity to specific ribohomopolymer." ;
  rdfs:subClassOf obo:ECO_0000138 , obo:ECO_0006083 .

obo:GO_0008152 a rdfs:Class ;
  rdfs:label "metabolic process" ;
  rdfs:subClassOf obo:GO_0008150 .

obo:OBI_0001025 a rdfs:Class ;
  rdfs:label "binding constant determination assay" ;
  rdfs:comment "A binding assay where the specified output is a binding constant" ;
  rdfs:subClassOf obo:OBI_0001146 .

obo:OBI_0001080 a rdfs:Class ;
  rdfs:label "patch clamp device" ;
  rdfs:comment "A device used in electrophysiology that allows the study of single or multiple ion channels in cells." ;
  rdfs:subClassOf obo:OBI_0000832 .

obo:ECO_0007407 a rdfs:Class ;
  rdfs:label "in vitro transcription assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro transcription assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001071 , obo:ECO_0007768 .

obo:GO_0051048 a rdfs:Class ;
  rdfs:label "negative regulation of secretion" ;
  rdfs:subClassOf obo:GO_0051046 , obo:GO_0051051 , obo:GO_0065007 .

obo:ECO_0001226 a rdfs:Class ;
  rdfs:label "lipid binding assay evidence used in manual assertion" ;
  rdfs:comment "A type of lipid binding assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000353 , obo:ECO_0001092 .

obo:ECO_0006164 a rdfs:Class ;
  rdfs:label "nuclear magnetic resonance spectroscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of nuclear magnetic resonance spectroscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006163 , obo:ECO_0007441 .

obo:ECO_0000232 a rdfs:Class ;
  rdfs:label "chromosome conformation-based evidence" ;
  rdfs:comment "A type of experimental evidence that is based on a five-step technique of cross-linking DNA in vivo, restriction digestion, intramolecular ligation, reversing DNA cross-links, DNA processing, and quantitation that serves to analyze the spatial organization of chromatin in a cell and quantify the number of interactions between genomic loci that are nearby in 3-D space." ;
  rdfs:subClassOf obo:BFO_0000002 , obo:ECO_0000006 .

obo:ECO_0008020 a rdfs:Class ;
  rdfs:label "string-matching method evidence used in manual assertion" ;
  rdfs:comment "A type of string-matching method evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007668 , obo:ECO_0008011 .

obo:OBI_0001684 a rdfs:Class ;
  rdfs:label "methylation-specific polymerase chain reaction assay" ;
  rdfs:comment "An assay that uses initial modification of DNA by sodium bisulfite, converting all unmethylated, but not methylated, cytosines to uracil, and subsequent amplification with primers specific for methylated versus unmethylated DNA." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:OBI_0000882 a rdfs:Class ;
  rdfs:label "cell-cell killing assay" ;
  rdfs:comment "A cytometry assay that monitors a cell population to track how many are killed by other cells." ;
  rdfs:subClassOf obo:OBI_0001977 .

obo:GO_0071715 a rdfs:Class ;
  rdfs:label "icosanoid transport" ;
  rdfs:subClassOf obo:GO_0046942 .

obo:ECO_0006277 a rdfs:Class ;
  rdfs:label "fluorescence polarization evidence used in manual assertion" ;
  rdfs:comment "A type of fluorescence polarization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000353 , obo:ECO_0006248 .

obo:ECO_0005659 a rdfs:Class ;
  rdfs:label "position-specific scoring matrix motif search evidence used in automatic assertion" ;
  rdfs:comment "A type of position-specific scoring matrix motif search evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000258 , obo:ECO_0005536 .

obo:OBI_0000669 a rdfs:Class ;
  rdfs:label "tritiated thymidine incorporation assay" ;
  rdfs:comment "A cell proliferation assay in which cells are cultured in the presence of tritiated thymidine which is incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle). The radioactivity of tritiated thymidine in a cell is a proxy for cells that were actively replicating." ;
  rdfs:subClassOf obo:OBI_0000891 .

obo:ECO_0001594 a rdfs:Class ;
  rdfs:label "polysome profiling evidence used in manual assertion" ;
  rdfs:comment "A type of polysome profiling evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001592 .

obo:OBI_0001313 a rdfs:Class ;
  rdfs:label "transcription profiling by RT-PCR design" ;
  rdfs:comment "A study design which aims to examine the transcriptome of a biological sample by reverse transcription PCR (RT-PCR)." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:GO_0045740 a rdfs:Class ;
  rdfs:label "positive regulation of DNA replication" ;
  rdfs:subClassOf obo:GO_0006275 , obo:GO_0010557 , obo:GO_0031328 , obo:GO_0065007 .

obo:ECO_0007699 a rdfs:Class ;
  rdfs:label "cell growth assay evidence used in manual assertion" ;
  rdfs:comment "A type of cell growth assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001563 , obo:ECO_0007695 .

obo:ECO_0007788 a rdfs:Class ;
  rdfs:label "sumoylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of sumoylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001559 , obo:ECO_0007307 .

obo:ECO_0000244 a rdfs:Class ;
  rdfs:label "combinatorial evidence used in manual assertion" ;
  rdfs:comment "A type of combinatorial analysis that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000212 , obo:ECO_0000352 .

obo:ECO_0005565 a rdfs:Class ;
  rdfs:label "obsolete single channel conductance evidence" .

obo:ECO_0000059 a rdfs:Class ;
  rdfs:label "experimental phenotypic evidence" ;
  rdfs:comment "A type of experimental evidence that is based on an observable characteristic trait, which is the result of the expression of an organisms genotype in an environment." ;
  rdfs:subClassOf obo:ECO_0000006 , obo:IAO_0000030 .

obo:ECO_0006052 a rdfs:Class ;
  rdfs:label "RNAi evidence used in manual assertion" ;
  rdfs:comment "A type of RNAi evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000019 , obo:ECO_0000315 .

obo:ECO_0007181 a rdfs:Class ;
  rdfs:label "cut-open oocyte voltage clamp recording evidence used in manual assertion" ;
  rdfs:comment "A type of cut-open oocyte voltage clamp recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005583 , obo:ECO_0006006 .

obo:OBI_0001506 a rdfs:Class ;
  rdfs:label "specimen with pre- or post-mortem status"@en ;
  rdfs:comment "A specimen that has been established to be taken from a live (pre-mortem) or dead (post-mortem) organism." ;
  rdfs:subClassOf obo:OBI_0100051 .

obo:ECO_0001026 a rdfs:Class ;
  rdfs:label "co-localization evidence" ;
  rdfs:comment "A type of imaging assay evidence resulting from the limited observation of the co-occurrence of molecules in a subcellular location." ;
  rdfs:subClassOf obo:ECO_0000324 .

obo:ECO_0006095 a rdfs:Class ;
  rdfs:label "double mutant phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of double mutant phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000054 , obo:ECO_0000315 .

obo:ECO_0007480 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence by experimental evidence from mixed species used in automatic assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005552 , obo:ECO_0007828 .

obo:OBI_1110126 a rdfs:Class ;
  rdfs:label "assay measuring binding of a MHC:ligand complex" ;
  rdfs:comment "A MHC ligand assay that detects the binding of a ligand to an MHC molecule." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_0002075 .

obo:ECO_0005034 a rdfs:Class ;
  rdfs:label "apoptotic assay evidence" ;
  rdfs:comment "A type of cell-based assay evidence resulting from the occurrence, visualization, and/or analysis of apoptosis." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:GO_2001141 a rdfs:Class ;
  rdfs:label "regulation of RNA biosynthetic process" ;
  rdfs:subClassOf obo:GO_0010556 , obo:GO_0031326 , obo:GO_0051252 , obo:GO_0065007 .

obo:ECO_0006303 a rdfs:Class ;
  rdfs:label "microscale thermophoresis evidence used in manual assertion" ;
  rdfs:comment "A type of microscale thermophoresis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000353 , obo:ECO_0001249 , obo:ECO_0006261 .

obo:GO_0090592 a rdfs:Class ;
  rdfs:label "DNA synthesis involved in DNA replication" ;
  rdfs:subClassOf obo:GO_0071897 .

obo:ECO_0001129 a rdfs:Class ;
  rdfs:label "TACE activity assay evidence" ;
  rdfs:comment "A type of enzymatic activity assay evidence resulting from the analysis of tumor necrosis factor (TNF)-a converting enzyme (TACE), which releases a soluble TNF-a from the membrane-bound precursor protein." ;
  rdfs:subClassOf obo:ECO_0000005 .

obo:ECO_0006028 a rdfs:Class ;
  rdfs:label "Kadouri drip-fed biofilm assay evidence" ;
  rdfs:comment "A type of biofilm formation assay evidence in which mature bacterial biofilms are grown in a multiwell plate that has a growth medium continually pumped through the wells while waste is continually pumped out." ;
  rdfs:subClassOf obo:ECO_0006020 .

obo:ECO_0007508 a rdfs:Class ;
  rdfs:label "fluorescence anisotropy evidence used in automatic assertion" ;
  rdfs:comment "A type of fluorescence anisotropy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001811 , obo:ECO_0007450 .

obo:ECO_0001808 a rdfs:Class ;
  rdfs:label "reverse transcription polymerase chain reaction evidence used in manual assertion" ;
  rdfs:comment "A type of reverse transcription polymerase chain reaction evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000109 , obo:ECO_0007739 .

obo:OBI_0002743 a rdfs:Class ;
  rdfs:label "molecular label role"@en ;
  rdfs:comment "A reagent role inhering in a molecular entity intended to associate with some molecular target to serve as a proxy for the presence, abundance, or location of this target in a detection of molecular label assay."@en ;
  rdfs:subClassOf obo:OBI_0000086 .

obo:ECO_0001100 a rdfs:Class ;
  rdfs:label "MTS assay evidence" ;
  rdfs:comment "A type of apoptotic assay evidence resulting from the reduction of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) in combination with phenazine methyl sulfate (PMS) as an intermediate electron acceptor reagent to produce a soluble formazan dye in viable cells." ;
  rdfs:subClassOf obo:ECO_0005034 .

obo:GO_0010033 a rdfs:Class ;
  rdfs:label "response to organic substance" ;
  rdfs:subClassOf obo:GO_0042221 .

obo:GO_0051054 a rdfs:Class ;
  rdfs:label "positive regulation of DNA metabolic process" ;
  rdfs:subClassOf obo:GO_0010604 , obo:GO_0045935 , obo:GO_0051052 , obo:GO_0065007 .

obo:ECO_0007273 a rdfs:Class ;
  rdfs:label "in-gel protein kinase assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in-gel protein kinase assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005562 , obo:ECO_0007767 .

obo:ECO_0001009 a rdfs:Class ;
  rdfs:label "allograft transplantation phenotypic evidence" ;
  rdfs:comment "A type of anatomical perturbation phenotypic evidence resulting from the transplantation of an organ or tissue from one individual of the same species with a different genotype." ;
  rdfs:subClassOf obo:ECO_0000240 .

obo:OBI_0000796 a rdfs:Class ;
  rdfs:label "purification objective" ;
  rdfs:comment "The objective to separate a material entity into different compositions of which one or more have are purified fractions that contain higher concentration of a desired component, while others contain impurities and are not of interest" ;
  rdfs:subClassOf obo:OBI_0000681 .

obo:ECO_0000204 a rdfs:Class ;
  rdfs:label "author statement" ;
  rdfs:comment "A type of documented statement evidence that is based on an assertion by the author of a paper, which is read by a curator." ;
  rdfs:subClassOf obo:ECO_0006151 .

obo:ECO_0001006 a rdfs:Class ;
  rdfs:label "adhesion assay evidence" ;
  rdfs:comment "A type of cell-based assay evidence resulting from the analysis of cell binding either to extracellular matrix proteins or other cells." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:ECO_0007321 a rdfs:Class ;
  rdfs:label "author statement supported by traceable reference used in automatic assertion" ;
  rdfs:comment "A type of author statement supported by traceable reference that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000033 , obo:ECO_0007319 .

obo:ECO_0007262 a rdfs:Class ;
  rdfs:label "obsolete visual sequence inspection evidence used in automatic assertion" ;
  rdfs:comment "OBSOLETE. A type of visual sequence inspection evidence that is used in an automatic assertion." .

obo:ECO_0007777 a rdfs:Class ;
  rdfs:label "apoptotic assay evidence used in automatic assertion" ;
  rdfs:comment "A type of apoptotic assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005034 , obo:ECO_0007773 .

obo:ECO_0000344 a rdfs:Class ;
  rdfs:label "partial support of intron positions by RNA-sequencing alignment evidence" ;
  rdfs:comment "A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignment to a genome supports only some of the intron positions (exon pairs) predicted for a transcript." ;
  rdfs:subClassOf obo:ECO_0000342 .

obo:ECO_0007448 a rdfs:Class ;
  rdfs:label "point mutation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of point mutation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001113 , obo:ECO_0007325 .

obo:ECO_0007026 a rdfs:Class ;
  rdfs:label "indirect Coombs test evidence used in manual assertion" ;
  rdfs:comment "A type of indirect Coombs test evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007020 , obo:ECO_0007025 .

obo:OBI_0000747 a rdfs:Class ;
  rdfs:label "material sample" ;
  rdfs:comment "A material entity that has the material sample role" ;
  rdfs:subClassOf obo:BFO_0000040 , obo:OBI_0100051 .

obo:ECO_0000162 a rdfs:Class ;
  rdfs:label "heterologous system uptake evidence" ;
  rdfs:comment "A type of transport assay evidence in which the uptake mechanism of a transporter is investigated in a cell that does not normally express that protein." ;
  rdfs:subClassOf obo:ECO_0000134 .

obo:GO_0016477 a rdfs:Class ;
  rdfs:label "cell migration" ;
  rdfs:subClassOf obo:GO_0048870 .

obo:OBI_1110109 a rdfs:Class ;
  rdfs:label "target of material addition" ;
  rdfs:comment "A material entity into which another is being added in a material combinatino process" ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:ECO_0006255 a rdfs:Class ;
  rdfs:label "deglycosylation assay evidence" ;
  rdfs:comment "A type of direct assay evidence where the removal of glycan groups from a substrate (RNA, DNA, or protein) is detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0001040 a rdfs:Class ;
  rdfs:label "RNA dot blot assay evidence" ;
  rdfs:comment "A type of RNA detection assay evidence based on the direct application of a RNA mixture in a circular motion on a matrix for hybridization with labeled DNA fragments." ;
  rdfs:subClassOf obo:ECO_0005518 .

obo:ECO_0006015 a rdfs:Class ;
  rdfs:label "whole-cell patch-clamp recording evidence used in manual assertion" ;
  rdfs:comment "A type of whole-cell patch-clamp recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006013 , obo:ECO_0006014 .

obo:GO_0015909 a rdfs:Class ;
  rdfs:label "long-chain fatty acid transport" ;
  rdfs:subClassOf obo:GO_0015908 .

obo:ECO_0007674 a rdfs:Class ;
  rdfs:label "manually integrated combinatorial evidence" ;
  rdfs:comment "A type of combinatorial evidence in which at least two distinct types of evidence have been integrated manually." ;
  rdfs:subClassOf obo:ECO_0000212 .

obo:OBI_0200031 a rdfs:Class ;
  rdfs:label "differential expression analysis objective"@en ;
  rdfs:comment "A differential expression analysis objective is a data transformation objective whose input consists of expression levels of entities (such as transcripts or proteins), or of sets of such expression levels, under two or more conditions and whose output reflects which of these are likely to have different expression across such conditions."@en ;
  rdfs:subClassOf obo:OBI_0200166 .

obo:OBI_0001200 a rdfs:Class ;
  rdfs:label "genotyping by high throughput sequencing design" ;
  rdfs:comment "A study design that classifies an individual or group of individuals on the basis of alleles, haplotypes, SNPs using high througput sequencing techniques." ;
  rdfs:subClassOf obo:OBI_0001444 .

obo:GO_0043412 a rdfs:Class ;
  rdfs:label "macromolecule modification" ;
  rdfs:subClassOf obo:GO_0043170 .

obo:GO_0050794 a rdfs:Class ;
  rdfs:label "regulation of cellular process" ;
  rdfs:subClassOf obo:GO_0050789 , obo:GO_0065007 .

obo:ECO_0007809 a rdfs:Class ;
  rdfs:label "nucleic acid localization evidence used in automatic assertion" ;
  rdfs:comment "A type of nucleic acid localization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001534 , obo:ECO_0007806 .

obo:ECO_0007310 a rdfs:Class ;
  rdfs:label "expression library screen evidence used in automatic assertion" ;
  rdfs:comment "A type of expression library screen evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000114 , obo:ECO_0007309 .

obo:ECO_0007295 a rdfs:Class ;
  rdfs:label "inference by association of genotype from phenotype used in automatic assertion" ;
  rdfs:comment "A type of inference by association of genotype from phenotype that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005613 , obo:ECO_0007191 , obo:ECO_0007440 .

obo:ECO_0007155 a rdfs:Class ;
  rdfs:label "differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) used in manual assertion" ;
  rdfs:comment "A type of differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000291 , obo:ECO_0000359 .

obo:ECO_0006046 a rdfs:Class ;
  rdfs:label "single-unit extracellular recording evidence used in manual assertion" ;
  rdfs:comment "A type of single-unit extracellular recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006044 , obo:ECO_0006045 .

obo:OBI_0000391 a rdfs:Class ;
  rdfs:label "energy supply function"@en ;
  rdfs:comment "An energy supply function is a function to supply or transfer energy from an energy source to a consumer of the energy"@en ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:CL_0000000 a rdfs:Class ;
  rdfs:label "cell" ;
  rdfs:comment "A material entity of anatomical origin (part of or deriving from an organism) that has as its parts a maximally connected cell compartment surrounded by a plasma membrane." ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:ECO_0000307 a rdfs:Class ;
  rdfs:label "no evidence data found used in manual assertion" ;
  rdfs:comment "An inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources that is used in an manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/nd-no-biological-data-available> ;
  rdfs:subClassOf obo:ECO_0000035 , obo:ECO_0000305 .

obo:ECO_0000292 a rdfs:Class ;
  rdfs:label "morpholino experiment evidence" ;
  rdfs:comment "A type of mutant phenotype evidence that uses anti-sense by introducing morpholino oligonucleotides into the cytosol of a cell." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0000226 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation evidence" ;
  rdfs:comment "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." ;
  rdfs:subClassOf obo:ECO_0000085 .

obo:GO_0033044 a rdfs:Class ;
  rdfs:label "regulation of chromosome organization" ;
  rdfs:subClassOf obo:GO_0033043 , obo:GO_0065007 .

obo:ECO_0006305 a rdfs:Class ;
  rdfs:label "native protein gel electrophoresis evidence used in manual assertion" ;
  rdfs:comment "A type of native protein gel electrophoresis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0006262 .

obo:ECO_0007556 a rdfs:Class ;
  rdfs:label "anti-sense experiment evidence used in automatic assertion" ;
  rdfs:comment "A type of anti-sense experiment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000018 , obo:ECO_0007325 .

obo:ECO_0007320 a rdfs:Class ;
  rdfs:label "author statement without traceable support used in automatic assertion" ;
  rdfs:comment "A type of author statement without traceable support that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000034 , obo:ECO_0007319 .

obo:ECO_0000319 a rdfs:Class ;
  rdfs:label "biological aspect of descendant evidence used in manual assertion" ;
  rdfs:comment "A type of biological aspect of descendant evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/ibd-inferred-biological-aspect-descendent> ;
  rdfs:subClassOf obo:ECO_0000214 , obo:ECO_0000252 .

obo:ECO_0007210 a rdfs:Class ;
  rdfs:label "green fluorescent protein fusion protein localization evidence used in automatic assertion" ;
  rdfs:comment "A type of green fluorescent protein fusion protein localization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000126 , obo:ECO_0007808 .

obo:OBI_0000057 a rdfs:Class ;
  rdfs:label "allotransplantation"@en ;
  rdfs:comment "is the transplantation of organs between members of the same species."@en ;
  rdfs:subClassOf obo:OBI_0000105 .

obo:ECO_0007520 a rdfs:Class ;
  rdfs:label "primer extension assay evidence used in automatic assertion" ;
  rdfs:comment "A type of primer extension assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001819 , obo:ECO_0007303 .

obo:ECO_0006244 a rdfs:Class ;
  rdfs:label "polyadenylated transcript 3'-end-sequencing evidence used in automatic assertion" ;
  rdfs:comment "A type of polyadenylated transcript 3'-end-sequencing evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006069 , obo:ECO_0006239 .

obo:ECO_0005803 a rdfs:Class ;
  rdfs:label "motility assay evidence" ;
  rdfs:comment "A type of direct assay evidence where the motility of a cell or cells is determined." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0005607 a rdfs:Class ;
  rdfs:label "tethered cell assay evidence" ;
  rdfs:comment "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by observing the rotational behavior of the cells tethered to a coverslip by their flagella." ;
  rdfs:subClassOf obo:ECO_0005021 .

obo:ECO_0001190 a rdfs:Class ;
  rdfs:label "in vitro acetylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro acetylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001057 .

obo:OBI_0000345 a rdfs:Class ;
  rdfs:label "mass analyzer" ;
  rdfs:comment "A Mass analyzer is a device that separates ions according to their mass-to-charge ratio. All mass spectrometers are based on dynamics of charged particles in electric and magnetic fields in vacuum where the two laws of Lorentz force law and Newton's second law of motion apply." ;
  rdfs:subClassOf obo:OBI_0000832 , obo:OBI_0000932 .

obo:ECO_0000359 a rdfs:Class ;
  rdfs:label "differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact)" ;
  rdfs:comment "A type of transcript expression evidence resulting from the use of the Gene Set Enrichment Analysis (GSEA) method or Fisher's-Exact (FE) method to assess an RNA-seq dataset for differential gene expression." ;
  rdfs:subClassOf obo:ECO_0000009 .

obo:ECO_0007241 a rdfs:Class ;
  rdfs:label "tissue ablation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of tissue ablation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000242 , obo:ECO_0007810 .

obo:ECO_0000345 a rdfs:Class ;
  rdfs:label "single exon transcript confirmation via alignment evidence" ;
  rdfs:comment "A type of sequence alignment evidence resulting from the comparison of a single exon transcript to a collection / database of known single exon genes." ;
  rdfs:subClassOf obo:ECO_0000200 .

obo:ECO_0001259 a rdfs:Class ;
  rdfs:label "syngeneic transplantation experiment evidence used in manual assertion" ;
  rdfs:comment "A type of syngeneic transplantation experiment evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001128 , obo:ECO_0007634 .

obo:ECO_0000081 a rdfs:Class ;
  rdfs:label "targeting sequence prediction evidence" ;
  rdfs:comment "A type of motif similarity evidence that is based on detection of a targeting sequence in the primary sequence of a protein through computational prediction and/or a manual examination of the sequence." ;
  rdfs:subClassOf obo:ECO_0000028 .

obo:GO_0015318 a rdfs:Class ;
  rdfs:label "inorganic molecular entity transmembrane transporter activity" ;
  rdfs:subClassOf obo:GO_0022857 .

obo:ECO_0000109 a rdfs:Class ;
  rdfs:label "reverse transcription polymerase chain reaction evidence" ;
  rdfs:comment "A type of RNA detection assay evidence where an RNA transcript is reverse transcribed into cDNA and amplified to qualitatively detect gene expression." ;
  rdfs:subClassOf obo:ECO_0005518 .

obo:ECO_0000034 a rdfs:Class ;
  rdfs:label "author statement without traceable support" ;
  rdfs:comment "A type of author statement that is not associated with results presented or a cited reference." ;
  rdfs:subClassOf obo:ECO_0000204 .

obo:ECO_0001245 a rdfs:Class ;
  rdfs:label "peptide array evidence used in manual assertion" ;
  rdfs:comment "A type of peptide array evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001112 , obo:ECO_0006076 .

obo:ECO_0001195 a rdfs:Class ;
  rdfs:label "in vitro desumoylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro desumoylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001063 .

obo:OBI_0000893 a rdfs:Class ;
  rdfs:label "real time polymerase chain reaction assay" ;
  rdfs:comment "An assay, based on the PCR, that amplifies and simultaneously quantifies a specific DNA molecule based on the use of complementary probes/primers. It enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample." ;
  rdfs:subClassOf obo:OBI_0002692 .

obo:GO_0061041 a rdfs:Class ;
  rdfs:label "regulation of wound healing" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_1903034 .

obo:ECO_0001583 a rdfs:Class ;
  rdfs:label "small interfering RNA knockdown evidence" ;
  rdfs:comment "A type of anti-sense experiment evidence where gene expression is disrupted through the introduction of double-stranded RNA molecules, 20-25 base pairs in length, which operate within the RNA interference pathway." ;
  rdfs:subClassOf obo:ECO_0000018 .

obo:ECO_0007129 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation-qPCR evidence used in manual assertion" ;
  rdfs:comment "A type of chromatin immunoprecipitation-qPCR evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000228 , obo:ECO_0005644 .

obo:OBI_1110014 a rdfs:Class ;
  rdfs:label "epitope binding by adaptive immune receptor"@en ;
  rdfs:comment "is the process in which an adaptive immune receptor binds to a material entity (realizing its disposition). The binding affinity is significant enough to trigger an immune response. Specifically, transient non-specific binding of adaptive immune receptors occurring during immune surveillance is not considered significant binding."@en ;
  rdfs:subClassOf obo:GO_0008150 .

obo:ECO_0001103 a rdfs:Class ;
  rdfs:label "natural variation mutant evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence resulting from the observation of the impact of a natural mutation on the expressed traits." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0001566 a rdfs:Class ;
  rdfs:label "quantitative reverse transcription polymerase chain reaction evidence" ;
  rdfs:comment "A type of reverse transcription polymerase chain reaction evidence that combines reverse transcription polymerase chain reaction and real time polymerase chain reaction to quantitatively assay for the detection of RNA levels." ;
  rdfs:subClassOf obo:ECO_0000109 .

obo:ECO_0007021 a rdfs:Class ;
  rdfs:label "slide agglutination test evidence" ;
  rdfs:comment "A type of agglutination test evidence that involves observaiton of visible clumping of antibody and antigen into a complex on a slide." ;
  rdfs:subClassOf obo:ECO_0007019 .

obo:ECO_0007170 a rdfs:Class ;
  rdfs:label "macroscopic current trace evidence used in manual assertion" ;
  rdfs:comment "A type of macroscopic current trace evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005563 , obo:ECO_0006006 .

obo:ECO_0000255 a rdfs:Class ;
  rdfs:label "match to sequence model evidence used in manual assertion" ;
  rdfs:comment "A type of match to sequence model evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/ism-inferred-sequence-model> ;
  rdfs:subClassOf obo:ECO_0000202 , obo:ECO_0000250 .

obo:ECO_0007454 a rdfs:Class ;
  rdfs:label "quantitative mass spectrometry evidence used in automatic assertion" ;
  rdfs:comment "A type of quantitative mass spectrometry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001119 , obo:ECO_0007433 .

obo:ECO_0006345 a rdfs:Class ;
  rdfs:label "asexual sporulation assay evidence" ;
  rdfs:comment "A type of sporulation assay evidence that reflects the presence, absence, and/or amount of asexual spore formation." ;
  rdfs:subClassOf obo:ECO_0006344 .

obo:ECO_0000291 a rdfs:Class ;
  rdfs:label "transcript expression evidence used in manual assertion" ;
  rdfs:comment "A type of transcript expression evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000009 , obo:ECO_0000270 .

obo:ECO_0001037 a rdfs:Class ;
  rdfs:label "4',6-diamidino-2-phenylindole staining evidence" ;
  rdfs:comment "A type of staining evidence resulting from 4',6-diamidino-2-phenylindole (DAPI) binding to AT regions of DNA and emitting a blue fluorescence." ;
  rdfs:subClassOf obo:ECO_0005019 .

obo:OBI_0600028 a rdfs:Class ;
  rdfs:label "artificially induced reverse transcription"@en ;
  rdfs:comment "a protocol with the objective to transcribe single-stranded RNA into complementary DNA (cDNA)"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0000074 a rdfs:Class ;
  rdfs:label "split-ubiquitin functional complementation evidence" ;
  rdfs:comment "A type of protein fragment functional complementation evidence that is based on detection of protein-protein interaction between a bait and prey protein by in vivo reconstitution of split-ubiquitin (when bait and prey interact) and release of a reporter protein." ;
  rdfs:subClassOf obo:ECO_0006256 .

obo:ECO_0007309 a rdfs:Class ;
  rdfs:label "protein expression evidence used in automatic assertion" ;
  rdfs:comment "A type of protein expression evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000010 , obo:ECO_0007298 .

obo:ECO_0007290 a rdfs:Class ;
  rdfs:label "tethered cell assay evidence used in automatic assertion" ;
  rdfs:comment "A type of tethered cell assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005607 , obo:ECO_0007780 .

obo:ECO_0006041 a rdfs:Class ;
  rdfs:label "genetically encoded fluorescent ion concentration sensor assay evidence used in manual assertion" ;
  rdfs:comment "A type of genetically encoded fluorescent ion concentration sensor assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006039 , obo:ECO_0006040 .

obo:GO_0060341 a rdfs:Class ;
  rdfs:label "regulation of cellular localization" ;
  rdfs:subClassOf obo:GO_0032879 , obo:GO_0050794 , obo:GO_0065007 .

obo:ECO_0005532 a rdfs:Class ;
  rdfs:label "consensus search evidence" ;
  rdfs:comment "A type of motif similarity evidence where the motif is represented by a consensus sequence which incorporates the most frequent nucleotide at each position acting as a generic representative for the sequences in the alignment, and is compared to the genetic sequence  for a perfect or imperfect match." ;
  rdfs:subClassOf obo:ECO_0000028 .

obo:ECO_0007584 a rdfs:Class ;
  rdfs:label "functional complementation in heterologous system evidence used in automatic assertion" ;
  rdfs:comment "A type of functional complementation in heterologous system evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000064 , obo:ECO_0007578 .

obo:ECO_0001111 a rdfs:Class ;
  rdfs:label "physical examination evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from the physical examination and measurement of the feature of a subject or sample." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0000106 a rdfs:Class ;
  rdfs:label "northern blot evidence" ;
  rdfs:comment "A type of transcript expression evidence based on electrophoresis and probing to determine levels of RNA expression using a complementary hybridization probe on the separated RNA samples." ;
  rdfs:subClassOf obo:ECO_0000009 , obo:IAO_0000030 .

obo:ECO_0000332 a rdfs:Class ;
  rdfs:label "whole transcript splice pattern evidence used in automatic assertion" ;
  rdfs:comment "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000327 , obo:ECO_0007825 .

obo:ECO_0005561 a rdfs:Class ;
  rdfs:label "pairwise sequence alignment evidence used in automatic assertion" ;
  rdfs:comment "A type of pairwise sequence alignment evidence that is used in an automatic evidence." ;
  rdfs:subClassOf obo:ECO_0000248 , obo:ECO_0005554 .

obo:ECO_0007461 a rdfs:Class ;
  rdfs:label "WST-1 assay evidence used in automatic assertion" ;
  rdfs:comment "A type of WST-1 assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001136 , obo:ECO_0007775 .

obo:ECO_0001264 a rdfs:Class ;
  rdfs:label "terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion" ;
  rdfs:comment "A type of terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001133 , obo:ECO_0007698 .

obo:CHEBI_53526 a rdfs:Class ;
  rdfs:label "tritiated thymidine" ;
  rdfs:comment "Thymidine linked to the radioisotope tritium. Used to label DNA in the study of cellular and viral DNA synthesis." ;
  rdfs:subClassOf obo:CHEBI_23367 .

obo:ECO_0007493 a rdfs:Class ;
  rdfs:label "electron microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of electron microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005033 , obo:ECO_0007435 , obo:ECO_0007815 .

obo:GO_0045182 a rdfs:Class ;
  rdfs:label "translation regulator activity" ;
  rdfs:subClassOf obo:GO_0003674 .

obo:ECO_0007054 a rdfs:Class ;
  rdfs:label "koehler illumination microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of koehler illumination microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001232 , obo:ECO_0007053 .

obo:OBI_0500001 a rdfs:Class ;
  rdfs:label "clinical study design" ;
  rdfs:comment "Plan for the precise procedure to be followed in a clinical trial, including planned and actual timing of events, choice of control group, method of allocating treatments, blinding methods; assigns a subject to pass through one or more epochs in the course of a trial. Specific design elements, e.g., crossover, parallel; dose-escalation [Modified from Pocock, Clinical Trials: A Practical Approach]" ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:ECO_0000136 a rdfs:Class ;
  rdfs:label "nucleic acid binding evidence" ;
  rdfs:comment "A type of affinity evidence resulting from the binding of a molecule to a nucleic acid." ;
  rdfs:subClassOf obo:ECO_0000023 .

obo:ECO_0007284 a rdfs:Class ;
  rdfs:label "cell-detached outside-out single-channel recording evidence used in automatic assertion" ;
  rdfs:comment "A type of cell-detached outside-out single-channel recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005582 , obo:ECO_0007537 .

obo:CL_0002319 a rdfs:Class ;
  rdfs:label "neural cell" ;
  rdfs:comment "A cell that is part of the nervous system." ;
  rdfs:subClassOf obo:CL_0000000 .

obo:OBI_0302729 a rdfs:Class ;
  rdfs:label "chemical solution"@en ;
  rdfs:comment "A material entity  that is made up of at least 2 scattered molecular aggregates, one playing the role of solvent and the other one playing the role of solute."@en ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:GO_0051345 a rdfs:Class ;
  rdfs:label "positive regulation of hydrolase activity" ;
  rdfs:subClassOf obo:GO_0043085 , obo:GO_0051336 , obo:GO_0065007 .

obo:ECO_0007336 a rdfs:Class ;
  rdfs:label "51Cr release assay evidence used in automatic assertion" ;
  rdfs:comment "A type of 51Cr release assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001004 , obo:ECO_0007781 .

obo:ECO_0007257 a rdfs:Class ;
  rdfs:label "phylogenetic distribution evidence used in automatic assertion" ;
  rdfs:comment "A type of phylogenetic distribution evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000355 , obo:ECO_0007826 .

obo:ECO_0000266 a rdfs:Class ;
  rdfs:label "sequence orthology evidence used in manual assertion" ;
  rdfs:comment "A type of sequence orthology evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/iso-inferred-sequence-orthology> ;
  rdfs:subClassOf obo:ECO_0000201 , obo:ECO_0000250 .

obo:ECO_0005669 a rdfs:Class ;
  rdfs:label "ultraviolet light footprinting evidence used in manual assertion" ;
  rdfs:comment "A type of ultraviolet light footprinting evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001826 , obo:ECO_0006083 .

obo:GO_0010558 a rdfs:Class ;
  rdfs:label "negative regulation of macromolecule biosynthetic process" ;
  rdfs:subClassOf obo:GO_0009890 , obo:GO_0010556 , obo:GO_0010605 , obo:GO_0065007 .

obo:GO_0098743 a rdfs:Class ;
  rdfs:label "cell aggregation" ;
  rdfs:subClassOf obo:GO_0009987 .

obo:ECO_0006004 a rdfs:Class ;
  rdfs:label "super-resolution microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of super-resolution microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001232 , obo:ECO_0005578 .

obo:ECO_0008007 a rdfs:Class ;
  rdfs:label "rule-based machine learning method evidence used in manual assertion" ;
  rdfs:comment "A type of rule-based machine learning method evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008002 , obo:ECO_0008003 .

obo:GO_0048856 a rdfs:Class ;
  rdfs:label "anatomical structure development" ;
  rdfs:subClassOf obo:GO_0032502 .

obo:OBI_0001879 a rdfs:Class ;
  rdfs:label "reagent"@en ;
  rdfs:comment "A biological or chemical entity that bears a reagent role in virtue of it being intended for application in a scientific technique  to participate in (or have molecular parts that participate in) a chemical reaction that facilitates the generation of data about some distinct entity, or the generation of some distinct material specified output." ;
  rdfs:subClassOf obo:BFO_0000040 , obo:OBI_0000047 .

obo:ECO_0006175 a rdfs:Class ;
  rdfs:label "nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence" ;
  rdfs:comment "A type of nuclear magnetic resonance spectroscopy evidence resulting from measuring the change in a certain type of NMR spectrum as a result of the spontenaous exchange of hydrogen and deuterium nuclei between the sample and the solvent." ;
  rdfs:subClassOf obo:ECO_0006163 .

obo:BFO_0000023 a rdfs:Class ;
  rdfs:label "role"@en ;
  rdfs:comment "A realizable entity  the manifestation of which brings about some result or end that is not essential to a continuant  in virtue of the kind of thing that it is but that can be served or participated in by that kind of continuant  in some kinds of natural, social or institutional contexts."@en ;
  rdfs:subClassOf obo:BFO_0000017 .

obo:ECO_0006089 a rdfs:Class ;
  rdfs:label "bacterial one-hybrid evidence used in manual assertion" ;
  rdfs:comment "A type of hybrid interaction evidence that is used in a manual assertion that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey)." ;
  rdfs:subClassOf obo:ECO_0000294 , obo:ECO_0006077 .

obo:GO_0060352 a rdfs:Class ;
  rdfs:label "cell adhesion molecule production" ;
  rdfs:subClassOf obo:GO_0009987 .

obo:ECO_0007790 a rdfs:Class ;
  rdfs:label "defarnesylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of defarnesylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001549 , obo:ECO_0007307 .

obo:ECO_0008031 a rdfs:Class ;
  rdfs:label "author inference based on intrinsic disorder prediction" ;
  rdfs:comment "A type of author inference in which information from intrinsic disorder prediction tools has been employed." ;
  rdfs:subClassOf obo:ECO_0000212 , obo:ECO_0006185 .

obo:ECO_0007525 a rdfs:Class ;
  rdfs:label "survival rate analysis evidence used in automatic assertion" ;
  rdfs:comment "A type of survival rate analysis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001822 , obo:ECO_0007370 .

obo:ECO_0007573 a rdfs:Class ;
  rdfs:label "protein:ion binding evidence used in automatic assertion" ;
  rdfs:comment "A type of protein:ion binding evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000142 , obo:ECO_0007373 .

obo:ECO_0001261 a rdfs:Class ;
  rdfs:label "WST-1 assay evidence used in manual assertion" ;
  rdfs:comment "A type of WST-1 assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001136 , obo:ECO_0007696 .

obo:OBI_0100010 a rdfs:Class ;
  rdfs:label "Epstein Barr virus transformed B cell"@en ;
  rdfs:comment "A material entity which results from viral transformation process using EBV as transformation agent when applied to B-cell entity"@en ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:GO_1903556 a rdfs:Class ;
  rdfs:label "negative regulation of tumor necrosis factor superfamily cytokine production" ;
  rdfs:subClassOf obo:GO_0001818 , obo:GO_0065007 , obo:GO_1903555 .

obo:ECO_0000233 a rdfs:Class ;
  rdfs:label "3C evidence" ;
  rdfs:comment "A type of chromosome conformation-based evidence that is derived by 1) quantifying interactions between a single pair of genomic loci and not multiple interactions and 2) performing polymerase chain reaction (PCR) in the final product quantitation step." ;
  rdfs:subClassOf obo:ECO_0000232 .

obo:ECO_0007065 a rdfs:Class ;
  rdfs:label "static light scattering assay evidence" ;
  rdfs:comment "A type of light scattering evidence derived by measuring the average intensity of scattered light by the particles at multiple angles." ;
  rdfs:subClassOf obo:ECO_0007061 .

obo:ECO_0007268 a rdfs:Class ;
  rdfs:label "duplication mutation evidence used in automatic assertion" ;
  rdfs:comment "A type of duplication mutation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005511 , obo:ECO_0007635 .

obo:ECO_0001212 a rdfs:Class ;
  rdfs:label "in vivo desumoylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo desumoylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001080 .

obo:OBI_0001290 a rdfs:Class ;
  rdfs:label "transcription profiling by array design" ;
  rdfs:comment "A study design that identifies forms and abundance of transcripts in the genome using microarray technology." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:ECO_0006385 a rdfs:Class ;
  rdfs:label "host-surrogate penetration assay evidence used in automatic assertion" ;
  rdfs:comment "A type of host-surrogate penetration assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006347 , obo:ECO_0007297 .

obo:ECO_0000254 a rdfs:Class ;
  rdfs:label "genetic similarity evidence used in automatic assertion" ;
  rdfs:comment "A type of genetic similarity evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000051 , obo:ECO_0000251 .

obo:ECO_0001818 a rdfs:Class ;
  rdfs:label "hydroxyl-radical footprinting evidence" ;
  rdfs:comment "A type of nucleic acid binding evidence used to identify protein-binding sites on the DNA molecule where DNA that has been bound to protein is digested with hydroxyl radical produced by reduction of hydrogen peroxide with iron (II), followed by separating the cleavage products on a denaturing electrophoresis gel." ;
  rdfs:subClassOf obo:ECO_0000136 .

obo:ECO_0006216 a rdfs:Class ;
  rdfs:label "author inference used in manual assertion" ;
  rdfs:comment "A type of author inference that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000302 , obo:ECO_0006185 , obo:ECO_0007751 .

obo:GO_0120035 a rdfs:Class ;
  rdfs:label "regulation of plasma membrane bounded cell projection organization" ;
  rdfs:subClassOf obo:GO_0031344 , obo:GO_0065007 .

obo:ECO_0001269 a rdfs:Class ;
  rdfs:label "surface plasmon resonance evidence used in manual assertion" ;
  rdfs:comment "A type of surface plasmon resonance evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000353 , obo:ECO_0001127 .

obo:OBI_0000639 a rdfs:Class ;
  rdfs:label "material separation objective" ;
  rdfs:comment "is an objective to transform a material entity into spatially separated components." ;
  rdfs:subClassOf obo:OBI_0000456 .

obo:GO_1903507 a rdfs:Class ;
  rdfs:label "negative regulation of nucleic acid-templated transcription" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_1902679 , obo:GO_1903506 .

obo:ECO_0006310 a rdfs:Class ;
  rdfs:label "competitive binding evidence used in automatic assertion" ;
  rdfs:comment "A type of competitive binding evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006264 , obo:ECO_0007384 .

obo:OBI_0000401 a rdfs:Class ;
  rdfs:label "environment control function"@en ;
  rdfs:comment "An environmental control function is a function that regulates a contained environment within specified parameter ranges. For example the control of light exposure, humidity and temperature."@en ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:ECO_0005514 a rdfs:Class ;
  rdfs:label "splice site mutation phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence resulting from a mutation in which there is a change in nucleotides at a splice site." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:GO_0008150 a rdfs:Class ;
  rdfs:label "biological_process" ;
  rdfs:comment "A biological process represents a specific objective that the organism is genetically programmed to achieve. Biological processes are often described by their outcome or ending state, e.g., the biological process of cell division results in the creation of two daughter cells (a divided cell) from a single parent cell. A biological process is accomplished by a particular set of molecular functions carried out by specific gene products (or macromolecular complexes), often in a highly regulated manner and in a particular temporal sequence." ;
  rdfs:subClassOf obo:BFO_0000015 .

obo:ECO_0008161 a rdfs:Class ;
  rdfs:label "antibody Y-3-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody Y-3-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008076 , obo:ECO_0008087 .

obo:ECO_0006378 a rdfs:Class ;
  rdfs:label "sporulation assay evidence used in manual assertion" ;
  rdfs:comment "A type of sporulation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0006344 .

obo:ECO_0001133 a rdfs:Class ;
  rdfs:label "terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence" ;
  rdfs:comment "A type of apoptotic assay evidence resulting from the visualization of DNA fragmentation by detection of exposed 3'-OH ends, localized by terminal deoxynucleotidyl transferase (TdT) which then catalyzes the addition of labeled dUTPs." ;
  rdfs:subClassOf obo:ECO_0005034 .

obo:ECO_0001533 a rdfs:Class ;
  rdfs:label "localization evidence" ;
  rdfs:comment "A type of direct assay evidence in which the sub-cellular location of a protein or nucleic acid sequence is determined." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:OBI_0000838 a rdfs:Class ;
  rdfs:label "material maintenance" ;
  rdfs:comment "a process with that achieves the objective to maintain some or all of the characteristics of an input material over time" ;
  rdfs:subClassOf obo:OBI_0000011 .

obo:ECO_0000301 a rdfs:Class ;
  rdfs:label "beta-galactosidase protein immunolocalization evidence" ;
  rdfs:comment "A type of immunolocalization evidence that was generated using LacZ protein as a marker." ;
  rdfs:subClassOf obo:ECO_0000087 .

obo:ECO_0001819 a rdfs:Class ;
  rdfs:label "primer extension assay evidence" ;
  rdfs:comment "A type of transcript expression evidence used to determine expression levels of mRNA where a labeled synthetic oligonucleotide primer is annealed to mRNA downstream of the presumed transcription start site of a gene. The mRNA is extended with reverse transcriptase, producing cDNA which is electrophoresed, and the band size is enables determination of the 5' end of the mRNA (i.e. the transcription start site)." ;
  rdfs:subClassOf obo:ECO_0000009 .

obo:ECO_0000177 a rdfs:Class ;
  rdfs:label "genomic context evidence" ;
  rdfs:comment "A type of similarity evidence in which the location of the gene within the genome provides insight into the gene's function or evolutionary insight." ;
  rdfs:subClassOf obo:ECO_0000041 .

obo:GO_0042575 a rdfs:Class ;
  rdfs:label "DNA polymerase complex" ;
  rdfs:comment "A protein complex that possesses DNA polymerase activity and is involved in template directed synthesis of DNA." ;
  rdfs:subClassOf obo:GO_0032991 , obo:OBI_0000427 .

obo:ECO_0007679 a rdfs:Class ;
  rdfs:label "combinatorial computational evidence used in automatic assertion" ;
  rdfs:comment "A type of combinatorial computational evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000213 , obo:ECO_0007677 .

obo:ECO_0007555 a rdfs:Class ;
  rdfs:label "field potential recording evidence used in automatic assertion" ;
  rdfs:comment "A type of field potential recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006047 , obo:ECO_0007553 .

obo:ECO_0000310 a rdfs:Class ;
  rdfs:label "nano-cap analysis of gene expression evidence" ;
  rdfs:comment "A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by reverse transcriptase template switching." ;
  rdfs:subClassOf obo:ECO_0000009 , obo:IAO_0000030 .

obo:GO_1990234 a rdfs:Class ;
  rdfs:label "transferase complex" ;
  rdfs:subClassOf obo:GO_1902494 .

obo:ECO_0007620 a rdfs:Class ;
  rdfs:label "colony papillation assay phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of colony papillation assay phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007067 , obo:ECO_0007625 .

obo:OBI_0001034 a rdfs:Class ;
  rdfs:label "environmental control device" ;
  rdfs:comment "An environmental control device is a device which has the function to control some aspect of the environment such as temperature, or humidity." ;
  rdfs:subClassOf obo:OBI_0000968 .

obo:ECO_0000281 a rdfs:Class ;
  rdfs:label "expression library screen evidence used in manual assertion" ;
  rdfs:comment "A type of expression library screen evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000114 , obo:ECO_0000284 .

obo:ECO_0007347 a rdfs:Class ;
  rdfs:label "blocking monoclonal antibody evidence used in automatic assertion" ;
  rdfs:comment "A type of blocking monoclonal antibody evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001013 , obo:ECO_0007348 .

obo:PATO_0001422 a rdfs:Class ;
  rdfs:label "dead" ;
  rdfs:comment "A viability quality inhering in a bearer by virtue of the cessation of the bearer's life." ;
  rdfs:subClassOf obo:PATO_0000169 .

obo:ECO_0001237 a rdfs:Class ;
  rdfs:label "natural variation mutant evidence used in manual assertion" ;
  rdfs:comment "A type of natural variation mutant evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001103 , obo:ECO_0007634 .

obo:ECO_0007643 a rdfs:Class ;
  rdfs:label "curator inference from Merriam-Webster Dictionary" ;
  rdfs:comment "A type of curator inference from dictionary in which the reference is to an entry in the Merriam-Webster Dictionary." ;
  rdfs:subClassOf obo:ECO_0007641 .

obo:ECO_0006367 a rdfs:Class ;
  rdfs:label "temperature jump relaxation evidence used in automatic assertion" ;
  rdfs:comment "A type of temperature jump relaxation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006338 , obo:ECO_0007450 .

obo:ECO_0006091 a rdfs:Class ;
  rdfs:label "functional complementation evidence used in manual assertion" ;
  rdfs:comment "A type of genetic interaction evidence that is used in a manual assertion where a wild-type copy of the gene in question is inserted into a mutant cell to see if it restores the wild-type phenotype in the mutant background." ;
  rdfs:subClassOf obo:ECO_0000012 , obo:ECO_0000316 .

obo:GO_0006306 a rdfs:Class ;
  rdfs:label "DNA methylation" ;
  rdfs:comment "The covalent transfer of a methyl group to either N-6 of adenine or C-5 or N-4 of cytosine." ;
  rdfs:subClassOf obo:GO_0006305 , obo:GO_0043414 , obo:GO_0044728 .

obo:GO_1904172 a rdfs:Class ;
  rdfs:label "positive regulation of bleb assembly" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_0120034 , obo:GO_1904170 .

obo:ECO_0007282 a rdfs:Class ;
  rdfs:label "reconstituted bilayer single-channel patch recording evidence used in automatic assertion" ;
  rdfs:comment "A type of reconstituted bilayer single-channel patch recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005575 , obo:ECO_0007537 .

obo:GO_0034220 a rdfs:Class ;
  rdfs:label "ion transmembrane transport" ;
  rdfs:subClassOf obo:GO_0006811 , obo:GO_0055085 .

obo:ECO_0000120 a rdfs:Class ;
  rdfs:label "over expression analysis evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence in which a gene and/or gene product is investigated in a transgenic organism that has been engineered to overexpress that gene product." ;
  rdfs:subClassOf obo:ECO_0005027 .

obo:ECO_0007547 a rdfs:Class ;
  rdfs:label "optogenetic evidence used in automatic assertion" ;
  rdfs:comment "A type of optogenetic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006032 , obo:ECO_0007635 .

obo:ECO_0000299 a rdfs:Class ;
  rdfs:label "cytochalasin experiment evidence" ;
  rdfs:comment "A type of cleavage arrested development that arises after treatment with cytochalasin." ;
  rdfs:subClassOf obo:ECO_0000298 .

obo:ECO_0005605 a rdfs:Class ;
  rdfs:label "disk diffusion test evidence" ;
  rdfs:comment "A type of zone of inhibition evidence where sensitivity/resistance to an antimicrobial agent is tested by impregnating small filter disks with a known concentration of antimicrobial agent which is then placed on a Mueller-Hinton agar plate inoculated with the test organism." ;
  rdfs:subClassOf obo:ECO_0007083 .

obo:ECO_0006080 a rdfs:Class ;
  rdfs:label "split-ubiquitin functional complementation evidence used in manual assertion" ;
  rdfs:comment "A type of split-ubiquitin functional complementation evidence that is used in a manual assertion that is based on detection of protein-protein interaction between a bait and prey protein by in vivo reconstitution of split-ubiquitin (when bait and prey interact) and release of a reporter protein." ;
  rdfs:subClassOf obo:ECO_0000074 , obo:ECO_0006293 .

obo:ECO_0006197 a rdfs:Class ;
  rdfs:label "nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence used in automatic assertion" ;
  rdfs:comment "A type of nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006164 , obo:ECO_0006175 .

obo:ECO_0001059 a rdfs:Class ;
  rdfs:label "in vitro deubiquitination assay evidence" ;
  rdfs:comment "A type of deubiquitination assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001552 .

obo:GO_0003674 a rdfs:Class ;
  rdfs:label "molecular_function" ;
  rdfs:comment "A molecular process that can be carried out by the action of a single macromolecular machine, usually via direct physical interactions with other molecular entities. Function in this sense denotes an action, or activity, that a gene product (or a complex) performs. These actions are described from two distinct but related perspectives: (1) biochemical activity, and (2) role as a component in a larger system/process." ;
  rdfs:subClassOf obo:BFO_0000015 .

obo:ECO_0006167 a rdfs:Class ;
  rdfs:label "nuclear magnetic resonance imaging evidence used in automatic assertion" ;
  rdfs:comment "A type of nuclear magnetic resonance imaging evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006166 , obo:ECO_0007441 .

obo:ECO_0000220 a rdfs:Class ;
  rdfs:label "sequencing assay evidence" ;
  rdfs:comment "A type of experimental evidence where the order of molecules that constitute a biopolymer is determined." ;
  rdfs:subClassOf obo:ECO_0000006 .

obo:OBI_0001271 a rdfs:Class ;
  rdfs:label "RNA-seq assay" ;
  rdfs:comment "An RNA sequencing assay that determines an RNA sequence by analyzing the transcibed regions of the genome and or to quantitate transcript abundance." ;
  rdfs:subClassOf obo:OBI_0001177 .

obo:ECO_0005578 a rdfs:Class ;
  rdfs:label "super-resolution microscopy evidence" ;
  rdfs:comment "A type of microscopy evidence where the images are taken with a higher resolution than the diffraction limit that help in studying nanoscopic sub-cellular structures." ;
  rdfs:subClassOf obo:ECO_0001098 .

obo:ECO_0007193 a rdfs:Class ;
  rdfs:label "loss-of-function mutant phenotype evidence used in automatic assertion" ;
  rdfs:comment "A type of loss-of-function mutant phenotype evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000016 , obo:ECO_0007325 .

obo:ECO_0007043 a rdfs:Class ;
  rdfs:label "transmission electron microscopy evidence" ;
  rdfs:comment "A type of electron microscopy evidence where a beam of electrons are transmitted through an ultra thin specimen forming an image which is magnified and focused onto an imaging device, such as a fluorescent screen, on a layer of photographic film, or to be detected by a sensor such as a CCD camera." ;
  rdfs:subClassOf obo:ECO_0005033 .

obo:ECO_0001174 a rdfs:Class ;
  rdfs:label "4',6-diamidino-2-phenylindole staining evidence used in manual assertion" ;
  rdfs:comment "A type of 4',6-diamidino-2-phenylindole staining evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001037 .

obo:OBI_0001914 a rdfs:Class ;
  rdfs:label "multiplex ligation-mediated amplification"@en ;
  rdfs:comment "A polymerase chain reaction that amplifies multiple targets with a single primer pair mediated by hybridization of a primer with its target sequence using ligation." ;
  rdfs:subClassOf obo:OBI_0000415 .

obo:ECO_0006063 a rdfs:Class ;
  rdfs:label "over expression analysis evidence used in manual assertion" ;
  rdfs:comment "A type of experimental phenotypic evidence that is used in a manual assertion where a gene and/or gene product is investigated in a transgenic organism that has been engineered to overexpress that gene product." ;
  rdfs:subClassOf obo:ECO_0000120 , obo:ECO_0006049 .

obo:ECO_0000300 a rdfs:Class ;
  rdfs:label "green fluorescent protein immunolocalization evidence" ;
  rdfs:comment "A type of immunolocalization evidence data that was generated using green fluorescent protein (GFP) as a marker." ;
  rdfs:subClassOf obo:ECO_0000087 .

obo:OBI_0001148 a rdfs:Class ;
  rdfs:label "gene knock out"@en ;
  rdfs:comment "a genetic transformation that renders a gene non-functional, e.g. due to a point mutation, or the removal of all, or part of, the gene using recombinant methods."@en ;
  rdfs:subClassOf obo:OBI_0600043 .

obo:ECO_0006248 a rdfs:Class ;
  rdfs:label "fluorescence polarization evidence" ;
  rdfs:comment "A type of physical interaction evidence based on the measurement of the change in the degree of polarization of a fluorophore upon binding to a molecular partner." ;
  rdfs:subClassOf obo:ECO_0000021 .

obo:GO_0032890 a rdfs:Class ;
  rdfs:label "regulation of organic acid transport" ;
  rdfs:subClassOf obo:GO_0051049 , obo:GO_0065007 .

obo:OBI_0000131 a rdfs:Class ;
  rdfs:label "molecular feature identification objective"@en ;
  rdfs:comment "Molecular_feature_identification_objective is a biological_feature_identification_objective role describing a study designed to examine or characterize molecular features of a biological system, e.g. expression profiling, copy number of molecular components, epigenetic modifications."@en ;
  rdfs:subClassOf obo:OBI_0000015 .

obo:ECO_0007459 a rdfs:Class ;
  rdfs:label "syngeneic transplantation experiment evidence used in automatic assertion" ;
  rdfs:comment "A type of syngeneic transplantation experiment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001128 , obo:ECO_0007635 .

obo:ECO_0001842 a rdfs:Class ;
  rdfs:label "random mutagenesis of specific target DNA evidence" ;
  rdfs:comment "A type of random mutagenesis phenotypic evidence resulting from the random mutation of a specifically targeted region of DNA." ;
  rdfs:subClassOf obo:ECO_0005529 .

obo:OBI_0000427 a rdfs:Class ;
  rdfs:label "enzyme"@en ;
  rdfs:comment """(protein or rna) or has_part (protein or rna) and
has_function some GO:0003824 (catalytic activity)"""@en ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:GO_0015267 a rdfs:Class ;
  rdfs:label "channel activity" ;
  rdfs:subClassOf obo:GO_0022803 .

obo:OBI_0600014 a rdfs:Class ;
  rdfs:label "material component separation"@en ;
  rdfs:comment "a material processing in which components of an input material become segregated in space"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:GO_0006807 a rdfs:Class ;
  rdfs:label "nitrogen compound metabolic process" ;
  rdfs:subClassOf obo:GO_0008152 .

obo:ECO_0006390 a rdfs:Class ;
  rdfs:label "bio-layer interferometry evidence used in manual assertion" ;
  rdfs:comment "A type of bio-layer interferometry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0006350 .

obo:ECO_0005655 a rdfs:Class ;
  rdfs:label "phylogenetic footprinting evidence used in automatic assertion" ;
  rdfs:comment "A type of phylogenetic footprinting evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005533 , obo:ECO_0005559 .

obo:ECO_0006314 a rdfs:Class ;
  rdfs:label "urea-induced protein unfolding evidence used in automatic assertion" ;
  rdfs:comment "A type of urea-induced protein unfolding evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006266 , obo:ECO_0006312 .

obo:GO_0043271 a rdfs:Class ;
  rdfs:label "negative regulation of ion transport" ;
  rdfs:subClassOf obo:GO_0043269 , obo:GO_0051051 , obo:GO_0065007 .

obo:ECO_0005673 a rdfs:Class ;
  rdfs:label "ad-hoc qualitative phenotype observation evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence where a non-standard trait (e.g. natural competence) is assessed qualitatively (e.g. presence/absence) and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:OBI_0000651 a rdfs:Class ;
  rdfs:label "urine specimen" ;
  rdfs:comment "a portion of urine collected from an organism" ;
  rdfs:subClassOf obo:OBI_0001479 .

obo:ECO_0006208 a rdfs:Class ;
  rdfs:label "cryogenic electron microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of cryogenic electron microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006003 , obo:ECO_0006181 .

obo:ECO_0007177 a rdfs:Class ;
  rdfs:label "cell-detached inside-out single-channel recording evidence used in manual assertion" ;
  rdfs:comment "A type of cell-detached inside-out single-channel recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005574 , obo:ECO_0006013 .

obo:GO_0032410 a rdfs:Class ;
  rdfs:label "negative regulation of transporter activity" ;
  rdfs:subClassOf obo:GO_0032409 , obo:GO_0044092 , obo:GO_0051051 , obo:GO_0065007 .

obo:GO_0051046 a rdfs:Class ;
  rdfs:label "regulation of secretion" ;
  rdfs:subClassOf obo:GO_0051049 , obo:GO_0065007 .

obo:ECO_0007134 a rdfs:Class ;
  rdfs:label "Hi-C evidence used in manual assertion" ;
  rdfs:comment "A type of Hi-C evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000238 , obo:ECO_0000269 .

obo:ECO_0006273 a rdfs:Class ;
  rdfs:label "dynamic fluorescence quenching evidence" ;
  rdfs:comment "A type of physical interaction evidence derived from the decrease (quenching) of fluorescence as a result of the interaction between a fluorescence donor and acceptor in their excited state." ;
  rdfs:subClassOf obo:ECO_0000021 , obo:ECO_0001115 .

obo:ECO_0008150 a rdfs:Class ;
  rdfs:label "antibody OX18-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody OX18-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008070 , obo:ECO_0008088 .

obo:ECO_0007015 a rdfs:Class ;
  rdfs:label "voltammetry evidence" ;
  rdfs:comment "A type of substance quantification evidence where an electrical gradient potential is applied to analyze an analyte of interest, resulting in a measurement amount of electrical current across the potential range applied." ;
  rdfs:subClassOf obo:ECO_0000335 .

obo:ECO_0006233 a rdfs:Class ;
  rdfs:label "differential scanning calorimetry evidence used in automatic assertion" ;
  rdfs:comment "A type of differential scanning calorimetry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006193 , obo:ECO_0006231 .

obo:OBI_0001051 a rdfs:Class ;
  rdfs:label "DNA extract" ;
  rdfs:comment "The output of an extraction process in which DNA molecules are purified in order to exclude DNA from organellas." ;
  rdfs:subClassOf obo:OBI_0001010 .

obo:OBI_0000471 a rdfs:Class ;
  rdfs:label "study design execution" ;
  rdfs:comment "a planned process that carries out a study design" ;
  rdfs:subClassOf obo:OBI_0000011 .

obo:ECO_0005630 a rdfs:Class ;
  rdfs:label "cDNA to DNA expression microarray evidence used in automatic assertion" ;
  rdfs:comment "A type of cDNA to DNA expression microarray evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000097 , obo:ECO_0007301 .

obo:ECO_0000182 a rdfs:Class ;
  rdfs:label "obsolete in vitro culture assay evidence" ;
  rdfs:comment "Use ECO:0001563 (cell growth assay evidence) in place of this term." .

obo:ECO_0007095 a rdfs:Class ;
  rdfs:label "quantitative trait analysis evidence used in manual assertion" ;
  rdfs:comment "A type of quantitative trait analysis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000061 , obo:ECO_0007634 .

obo:ECO_0006072 a rdfs:Class ;
  rdfs:label "immunofluorescence super resolution microscopy evidence" ;
  rdfs:comment "A type of super-resolution microscopy evidence resulting from the use of fluorescently-tagged antibodies to identify the localization of antigens in cells and tissue." ;
  rdfs:subClassOf obo:ECO_0005578 .

obo:ECO_0000110 a rdfs:Class ;
  rdfs:label "RNA protection assay evidence" ;
  rdfs:comment "A type of nuclease protection assay evidence where mRNA is hybridized with radiolabeled RNA probes after which RNAse is added to digest the unbound, nonresistant single-stranded overhang regions, later the remaining probe target hybrids are purified and resolved on denaturing polyacrylamide gel, and quantified by autoradiography." ;
  rdfs:subClassOf obo:ECO_0001106 .

obo:ECO_0006266 a rdfs:Class ;
  rdfs:label "urea-induced protein unfolding evidence" ;
  rdfs:comment "A type of protein unfolding evidence where the unfolding of the protein is triggered by increasing concentrations of urea." ;
  rdfs:subClassOf obo:ECO_0006265 .

obo:OBI_0002448 a rdfs:Class ;
  rdfs:label "differential screening hybridization" ;
  rdfs:comment "An artificially induced nucleic acid hybridization that is performed to identify differentially expressed genes through hybridization of cDNA probes." ;
  rdfs:subClassOf obo:OBI_0000253 .

obo:BFO_0000016 a rdfs:Class ;
  rdfs:label "disposition"@en ;
  rdfs:subClassOf obo:BFO_0000017 .

obo:ECO_0007145 a rdfs:Class ;
  rdfs:label "nano-cap analysis of gene expression evidence used in manual assertion" ;
  rdfs:comment "A type of nano-cap analysis of gene expression evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000291 , obo:ECO_0000310 .

obo:ECO_0007188 a rdfs:Class ;
  rdfs:label "capillary assay evidence used in manual assertion" ;
  rdfs:comment "A type of capillary assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005609 , obo:ECO_0007701 .

obo:GO_0045759 a rdfs:Class ;
  rdfs:label "negative regulation of action potential" ;
  rdfs:subClassOf obo:GO_0048519 , obo:GO_0065007 , obo:GO_0098900 .

obo:GO_0015711 a rdfs:Class ;
  rdfs:label "organic anion transport" ;
  rdfs:subClassOf obo:GO_0006820 , obo:GO_0071702 .

obo:ECO_0006037 a rdfs:Class ;
  rdfs:label "genetically encoded fluorescent sensor evidence used in manual assertion" ;
  rdfs:comment "A type of genetically encoded fluorescent sensor evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006035 , obo:ECO_0006036 .

obo:OBI_0001673 a rdfs:Class ;
  rdfs:label "nano-cap analysis of gene expression assay" ;
  rdfs:comment "A transcription profiling assay that is performed using a very low amount (nanogram scale) of mRNA samples using Cap analysis gene expression (CAGE)." ;
  rdfs:subClassOf obo:OBI_0000424 .

obo:ECO_0000092 a rdfs:Class ;
  rdfs:label "epitope-tagged protein immunolocalization evidence" ;
  rdfs:comment "A type of immunolocalization evidence in which recombinant proteins fused with epitopes are recognized by antibodies." ;
  rdfs:subClassOf obo:ECO_0000087 .

obo:ECO_0007632 a rdfs:Class ;
  rdfs:label "transcriptional activation assay evidence used in manual assertion" ;
  rdfs:comment "A type of transcriptional activation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000170 , obo:ECO_0000314 .

obo:ECO_0007504 a rdfs:Class ;
  rdfs:label "copper-phenanthroline footprinting evidence used in automatic assertion" ;
  rdfs:comment "A type of copper-phenanthroline footprinting evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001815 , obo:ECO_0007505 .

obo:GO_0048589 a rdfs:Class ;
  rdfs:label "developmental growth" ;
  rdfs:subClassOf obo:GO_0032502 , obo:GO_0040007 .

obo:ECO_0007444 a rdfs:Class ;
  rdfs:label "phosphoamino acid analysis evidence used in automatic assertion" ;
  rdfs:comment "A type of phosphoamino acid analysis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001109 , obo:ECO_0007307 .

obo:ECO_0007396 a rdfs:Class ;
  rdfs:label "in vitro defarnesylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro defarnesylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001061 , obo:ECO_0007790 .

obo:ECO_0001223 a rdfs:Class ;
  rdfs:label "obsolete induced mutation evidence used in manual assertion" ;
  rdfs:comment "OBSOLETE. A type of induced mutation evidence that is used in a manual assertion." .

obo:GO_1902742 a rdfs:Class ;
  rdfs:label "apoptotic process involved in development" ;
  rdfs:subClassOf obo:GO_0006915 .

obo:OBI_0001588 a rdfs:Class ;
  rdfs:label "binding" ;
  rdfs:comment "The process of material entities forming complexes. " ;
  rdfs:subClassOf obo:GO_0008150 .

obo:OBI_0001954 a rdfs:Class ;
  rdfs:label "ChIP assay" ;
  rdfs:comment "An assay in which portions of chromatin, the ordered and organized complex of DNA and its interaction partners that make up a chormosome, is extracted and purified by immunoprecipitation with antibodies or tags, and subsequently analyzed" ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0006400 a rdfs:Class ;
  rdfs:label "antibody Tu36-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody Tu36-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006398 , obo:ECO_0008087 .

obo:ECO_0007712 a rdfs:Class ;
  rdfs:label "palmitoylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of palmitoylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001555 .

obo:ECO_0006017 a rdfs:Class ;
  rdfs:label "author statement supported from referenced clinical study used in manual assertion" ;
  rdfs:comment "A type of author statement supported from referenced clinical study that is used in a manual assertion." ;
  rdfs:seeAlso <http://human-phenotype-ontology.github.io/documentation.html#annot> ;
  rdfs:subClassOf obo:ECO_0000304 , obo:ECO_0006016 .

obo:ECO_0007708 a rdfs:Class ;
  rdfs:label "sumoylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of sumoylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001559 .

obo:ECO_0007325 a rdfs:Class ;
  rdfs:label "mutant phenotype evidence used in automatic assertion" ;
  rdfs:comment "A type of mutant phenotype evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000015 , obo:ECO_0007635 .

obo:OBI_0000272 a rdfs:Class ;
  rdfs:label "protocol"@en ;
  rdfs:comment "A plan specification which has sufficient level of detail and quantitative information to communicate it between investigation agents, so that different investigation agents will reliably be able to independently reproduce the process."@en ;
  rdfs:subClassOf obo:IAO_0000104 .

obo:GO_0006869 a rdfs:Class ;
  rdfs:label "lipid transport" ;
  rdfs:subClassOf obo:GO_0071702 .

obo:ECO_0006282 a rdfs:Class ;
  rdfs:label "rotary shadowing electron microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of rotary shadowing electron microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006250 , obo:ECO_0007493 .

obo:IAO_0000030 a rdfs:Class ;
  rdfs:label "information content entity"@en ;
  rdfs:comment "A generically dependent continuant that is about some thing."@en ;
  rdfs:subClassOf obo:BFO_0000031 .

obo:ECO_0007230 a rdfs:Class ;
  rdfs:label "454 pyrosequencing evidence used in automatic assertion" ;
  rdfs:comment "A type of 454 pyrosequencing evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000223 , obo:ECO_0007814 .

obo:CHEBI_15422 a rdfs:Class ;
  rdfs:label "ATP" ;
  rdfs:comment "An adenosine 5'-phosphate in which the 5'-phosphate is a triphosphate group. It is involved in the transportation of chemical energy during metabolic pathways." ;
  rdfs:subClassOf obo:CHEBI_23367 .

obo:ECO_0005641 a rdfs:Class ;
  rdfs:label "beta-glucuronidase reporter gene assay evidence used in manual assertion" ;
  rdfs:comment "A type of beta-glucuronidase reporter gene assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000270 , obo:ECO_0001804 .

obo:OBI_0002179 a rdfs:Class ;
  rdfs:label "cell-attached patch clamp assay" ;
  rdfs:comment "A patch-clamp assay where the electrode is left in place on the cell, and the membrane patch has been left intact been perforated. This maintains the separation of the interior of the pipette to the intracellular space of the cell. Measurements made with this technique involve recording currents through multiple channels simultaneously, over the membrane of the entire cell." ;
  rdfs:subClassOf obo:OBI_0002177 .

obo:ECO_0006309 a rdfs:Class ;
  rdfs:label "competitive binding evidence used in manual assertion" ;
  rdfs:comment "A type of competitive binding evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006075 , obo:ECO_0006264 .

obo:ECO_0001820 a rdfs:Class ;
  rdfs:label "rapid amplification of cDNA ends polymerase chain reaction evidence" ;
  rdfs:comment "A type of nucleotide sequencing assay evidence in which RT-PCR (cDNA synthesis) is first used to produce a cDNA copy of a region of the RNA transcript being investigated followed by PCR to capture either the unknown 5' or 3' end of the transcript for sequencing, depending on whether 5' RACE-PCR or 3' RACE-PCR are being undertaken." ;
  rdfs:subClassOf obo:BFO_0000002 , obo:ECO_0000219 .

obo:OBI_0003145 a rdfs:Class ;
  rdfs:label "assay measuring quantitative binding of a MHC:ligand complex" ;
  rdfs:comment "An assay that detects the binding of a MHC molecule with a ligand, and produces a quantitative measurement of the binding as an output." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_1110126 .

obo:ECO_0007622 a rdfs:Class ;
  rdfs:label "flow cell biofilm assay evidence used in automatic assertion" ;
  rdfs:comment "A type of flow cell biofilm assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007071 , obo:ECO_0007541 .

obo:ECO_0007353 a rdfs:Class ;
  rdfs:label "bromodeoxyuridine incorporation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of bromodeoxyuridine incorporation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001018 , obo:ECO_0007354 .

obo:OBI_0000341 a rdfs:Class ;
  rdfs:label "histological sample preparation"@en ;
  rdfs:comment "histological sample preparation is the preparation of an input tissue via slicing and labeling to make tissue microstructure of interest visible in a future histology assay"@en ;
  rdfs:subClassOf obo:OBI_0000094 , obo:OBI_0000659 .

obo:ECO_0006169 a rdfs:Class ;
  rdfs:label "quantitative western immunoblotting evidence" ;
  rdfs:comment "A type of qualitative western immunoblotting evidence where detection of the signal is carried out through digital imaging and the signal is normalized with methods such as standard curves built from reference housekeeping proteins or total transferred protein measurements." ;
  rdfs:subClassOf obo:ECO_0000112 .

obo:ECO_0006186 a rdfs:Class ;
  rdfs:label "combinatorial experimental and author inference evidence contained in single publication" ;
  rdfs:comment "A type of combinatorial experimental and author inference evidence where the experimental results and author inference are contained in a single publication." ;
  rdfs:subClassOf obo:ECO_0007011 .

obo:OBI_0002035 a rdfs:Class ;
  rdfs:label "peptide mass fingerprinting assay" ;
  rdfs:comment "A mass spectrometry assay that measures the absolute mass of the cleaved peptides derived from an unknown protein of interest. These masses are then compared to values for known protein sequences to identify the unknown protein." ;
  rdfs:subClassOf obo:OBI_0000470 .

obo:ECO_0006061 a rdfs:Class ;
  rdfs:label "immunofluorescence wide-field microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of wide-field fluorescence microscopy evidence used in a manual assertion where fluorescently tagged antibodies are imaged that are used to bind to their antigens." ;
  rdfs:subClassOf obo:ECO_0005599 , obo:ECO_0005804 , obo:ECO_0006062 .

obo:ECO_0007199 a rdfs:Class ;
  rdfs:label "quantitative trait analysis evidence used in automatic assertion" ;
  rdfs:comment "A type of quantitative trait analysis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000061 , obo:ECO_0007635 .

obo:GO_0050482 a rdfs:Class ;
  rdfs:label "arachidonic acid secretion" ;
  rdfs:subClassOf obo:GO_0032309 , obo:GO_1903963 .

obo:ECO_0001048 a rdfs:Class ;
  rdfs:label "fluorescence resonance energy transfer evidence" ;
  rdfs:comment "A type of dynamic fluorescence quenching evidence resulting from the measurement of the proximity of two fluorophores, where the energy from an excited molecular fluorophore to another fluorophore can only occur within ~10nm." ;
  rdfs:subClassOf obo:ECO_0006273 .

obo:ECO_0007433 a rdfs:Class ;
  rdfs:label "mass spectrometry evidence used in automatic assertion" ;
  rdfs:comment "A type of mass spectrometry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001096 , obo:ECO_0007821 .

obo:OBI_0000370 a rdfs:Class ;
  rdfs:label "contain function"@en ;
  rdfs:comment "A contain function is a function to constrain a material entities location in space"@en ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:ECO_0007159 a rdfs:Class ;
  rdfs:label "ATP bioluminescence assay evidence used in manual assertion" ;
  rdfs:comment "A type of ATP bioluminescence assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005011 , obo:ECO_0007703 .

obo:ECO_0007766 a rdfs:Class ;
  rdfs:label "voltage clamp recording evidence used in automatic assertion" ;
  rdfs:comment "A type of voltage clamp recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005576 , obo:ECO_0007535 .

obo:ECO_0000142 a rdfs:Class ;
  rdfs:label "protein:ion binding evidence" ;
  rdfs:comment "A type of protein binding evidence resulting from a metal ion binding to a protein at a specific binding site." ;
  rdfs:subClassOf obo:ECO_0000024 .

obo:ECO_0006074 a rdfs:Class ;
  rdfs:label "co-purification evidence used in manual assertion" ;
  rdfs:comment "A type of physical interaction evidence that is used in manual assertion where a cellular component subunit is isolated as part of purification of its larger complex." ;
  rdfs:subClassOf obo:ECO_0000022 , obo:ECO_0000353 .

obo:OBI_0000419 a rdfs:Class ;
  rdfs:label "reverse transcriptase"@en ;
  rdfs:comment """enzyme and has_function some GO:0003964 (RNA-directed DNA polymerase
activity)"""@en ;
  rdfs:subClassOf obo:OBI_0000427 .

obo:ECO_0008172 a rdfs:Class ;
  rdfs:label "MHC complex binding prediction based MHC restriction evidence used in automatic assertion" ;
  rdfs:comment "A type of MHC complex binding prediction based MHC restriction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007669 , obo:ECO_0008081 .

obo:ECO_0001136 a rdfs:Class ;
  rdfs:label "WST-1 assay evidence" ;
  rdfs:comment "A type of cell proliferation assay evidence resulting from the assessment of metabolic activity from a water-soluble tetrazolium salt WST-1 being reduced outside the cell (by reacting with mitochondrial succinate-tetrazolium reductase) to form a formazan dye." ;
  rdfs:subClassOf obo:ECO_0005007 .

obo:ECO_0001114 a rdfs:Class ;
  rdfs:label "propidium iodide staining evidence" ;
  rdfs:comment "A type of staining evidence resulting from the binding and labeling of DNA (during apoptosis, when there is a loss of nuclear DNA content) with propidium iodide (PI) to identify the cells from which it originated." ;
  rdfs:subClassOf obo:ECO_0005019 .

obo:GO_0044271 a rdfs:Class ;
  rdfs:label "cellular nitrogen compound biosynthetic process" ;
  rdfs:subClassOf obo:GO_0034641 , obo:GO_0044249 .

obo:ECO_0001078 a rdfs:Class ;
  rdfs:label "in vivo demethylation assay evidence" ;
  rdfs:comment "A type of demethylation assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001550 .

obo:ECO_0005580 a rdfs:Class ;
  rdfs:label "flow cytometry evidence used in manual assertion" ;
  rdfs:comment "A type of flow cytometry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000268 , obo:ECO_0000314 .

obo:OBI_0600064 a rdfs:Class ;
  rdfs:label "recombinant vector cloning"@en ;
  rdfs:comment "a planned process with the objective to insert genetic material into a cloning vector for future replication of the inserted material"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:GO_1903035 a rdfs:Class ;
  rdfs:label "negative regulation of response to wounding" ;
  rdfs:subClassOf obo:GO_0048585 , obo:GO_0065007 , obo:GO_1903034 .

obo:PATO_0001995 a rdfs:Class ;
  rdfs:label "organismal quality" ;
  rdfs:comment "A quality that inheres in an entire organism or part of an organism." ;
  rdfs:subClassOf obo:PATO_0001241 .

obo:ECO_0007119 a rdfs:Class ;
  rdfs:label "in vivo assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000178 , obo:ECO_0000314 .

obo:ECO_0001831 a rdfs:Class ;
  rdfs:label "host colonization assay evidence used in automatic assertion" ;
  rdfs:comment "A type of host colonization assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001830 , obo:ECO_0007297 .

obo:OBI_0000740 a rdfs:Class ;
  rdfs:label "material sample role" ;
  rdfs:comment "A material sample role is a specimen role borne by a material entity that is the output of a material sampling process." ;
  rdfs:subClassOf obo:OBI_0000112 .

obo:ECO_0001107 a rdfs:Class ;
  rdfs:label "nucleotide analog incorporation assay evidence" ;
  rdfs:comment "A type of DNA synthesis cell proliferation assay evidence resulting from the analysis and measurement of the incorporation of fluorescently labeled nucleotide analog(s) into the DNA." ;
  rdfs:subClassOf obo:ECO_0005008 .

obo:ECO_0007688 a rdfs:Class ;
  rdfs:label "gel electrophoresis evidence used in manual assertion" ;
  rdfs:comment "A type of gel electrophoresis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0000337 .

obo:OBI_0001912 a rdfs:Class ;
  rdfs:label "cell freezing medium"@en ;
  rdfs:comment "A processed material that serves as a liquid vehicle for freezing cells for long term quiescent stroage, which contains chemicls needed to sustain cell viability across freeze-thaw cycles. " ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:GO_0040012 a rdfs:Class ;
  rdfs:label "regulation of locomotion" ;
  rdfs:subClassOf obo:GO_0050789 , obo:GO_0065007 .

obo:GO_0080090 a rdfs:Class ;
  rdfs:label "regulation of primary metabolic process" ;
  rdfs:subClassOf obo:GO_0019222 , obo:GO_0065007 .

obo:ECO_0007364 a rdfs:Class ;
  rdfs:label "co-sedimentation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of co-sedimentation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001028 , obo:ECO_0007332 .

obo:ECO_0007413 a rdfs:Class ;
  rdfs:label "in vivo defarnesylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo defarnesylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001077 , obo:ECO_0007790 .

obo:ECO_0001547 a rdfs:Class ;
  rdfs:label "protein cleavage assay evidence" ;
  rdfs:comment "A type of direct assay evidence where the cleavage of a protein into protein fragments by a protease is detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:GO_0008284 a rdfs:Class ;
  rdfs:label "positive regulation of cell population proliferation" ;
  rdfs:subClassOf obo:GO_0042127 , obo:GO_0048522 , obo:GO_0065007 .

obo:ECO_0005632 a rdfs:Class ;
  rdfs:label "fluorescence anisotropy evidence used in manual assertion" ;
  rdfs:comment "A type of fluorescence anisotropy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001249 , obo:ECO_0001811 .

obo:GO_2000573 a rdfs:Class ;
  rdfs:label "positive regulation of DNA biosynthetic process" ;
  rdfs:subClassOf obo:GO_0010557 , obo:GO_0031328 , obo:GO_0051054 , obo:GO_0065007 , obo:GO_2000278 .

obo:OBI_0001152 a rdfs:Class ;
  rdfs:label "transfection"@en ;
  rdfs:comment "a genetic transformation which relies on the use of physical, electrical and chemical phenomena to introduce DNA or RNA into a cell"@en ;
  rdfs:subClassOf obo:OBI_0600043 .

obo:ECO_0007166 a rdfs:Class ;
  rdfs:label "repeat expansion mutation phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of repeat expansion mutation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0005513 .

obo:GO_1900119 a rdfs:Class ;
  rdfs:label "positive regulation of execution phase of apoptosis" ;
  rdfs:subClassOf obo:GO_0043065 , obo:GO_0065007 , obo:GO_1900117 .

obo:ECO_0007638 a rdfs:Class ;
  rdfs:label "curator inference from Wikipedia" ;
  rdfs:comment "A type of curator inference from encyclopedia where the reference is to a Wikipedia article." ;
  rdfs:subClassOf obo:ECO_0007637 .

obo:ECO_0000132 a rdfs:Class ;
  rdfs:label "beta-galactosidase fusion protein localization evidence" ;
  rdfs:comment "A type of fusion protein localization evidence resulting from the targeted insertion of the beta-galactosidase coding gene (lacZ) into a specific gene to create a beta-galactosidase-tagged fusion protein." ;
  rdfs:subClassOf obo:ECO_0000124 .

obo:GO_0005215 a rdfs:Class ;
  rdfs:label "transporter activity" ;
  rdfs:subClassOf obo:GO_0003674 .

obo:ECO_0000070 a rdfs:Class ;
  rdfs:label "co-immunoprecipitation evidence" ;
  rdfs:comment "A type of immunoprecipitation evidence that involves precipitating two or more proteins via binding to an antibody specific to a single protein, followed by protein identification." ;
  rdfs:subClassOf obo:ECO_0000085 .

obo:OBI_0001925 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation with exonuclease sequencing assay" ;
  rdfs:comment "A ChIP-seq assay which uses immunoprecipitation to isolate protein bound DNA followed by an exonuclease step to degrade DNA that is not protein bound to provide greater resolution of the DNA binding site" ;
  rdfs:subClassOf obo:OBI_0000716 .

obo:ECO_0007028 a rdfs:Class ;
  rdfs:label "direct hemagglutination assay evidence used in manual assertion" ;
  rdfs:comment "A type of direct hemagglutination assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007020 , obo:ECO_0007027 .

obo:ECO_0007305 a rdfs:Class ;
  rdfs:label "array-based sequence capture evidence used in automatic assertion" ;
  rdfs:comment "A type of array-based sequence capture evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000093 , obo:ECO_0007817 .

obo:OBI_0002590 a rdfs:Class ;
  rdfs:label "molecular interaction identification design" ;
  rdfs:comment "A study design that investigates the features of interaction between molecules." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:ECO_0001216 a rdfs:Class ;
  rdfs:label "in vivo palmitoylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo palmitoylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001083 .

obo:ECO_0007058 a rdfs:Class ;
  rdfs:label "extended field laser confocal microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of extended field laser confocal microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005589 , obo:ECO_0007057 .

obo:OBI_0000966 a rdfs:Class ;
  rdfs:label "in live organism assay" ;
  rdfs:comment "An assay in which a measurement is made by observing entities located in an organism." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007536 a rdfs:Class ;
  rdfs:label "mRNA interactome capture evidence used in automatic assertion" ;
  rdfs:comment "A type of mRNA interactome capture evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006010 , obo:ECO_0007505 .

obo:ECO_0006181 a rdfs:Class ;
  rdfs:label "cryogenic electron microscopy evidence" ;
  rdfs:comment "A type of electron microscopy evidence where the sample being studied has been cooled to cryogenic temperatures (typically below -153 degrees Celsius) prior to the experiment." ;
  rdfs:subClassOf obo:ECO_0005033 .

obo:GO_0032414 a rdfs:Class ;
  rdfs:label "positive regulation of ion transmembrane transporter activity" ;
  rdfs:subClassOf obo:GO_0032411 , obo:GO_0032412 , obo:GO_0034767 , obo:GO_0065007 .

obo:ECO_0007271 a rdfs:Class ;
  rdfs:label "splice site mutation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of splice site mutation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005514 , obo:ECO_0007325 .

obo:OBI_0002754 a rdfs:Class ;
  rdfs:label "cDNA library"@en ;
  rdfs:comment "A collection of DNA molecules with sequences complementary to a specified set of mRNA molecules and commonly developed by enzymatic reverse transcription."@en ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:ECO_0005536 a rdfs:Class ;
  rdfs:label "position-specific scoring matrix motif search evidence" ;
  rdfs:comment "A type of motif similarity evidence where the motif is represented by a position-specific frequency matrix (PSFM) that specifies the frequency of each base at each position of the motif, from which a position-specific scoring matrix (PSSM) can be derived under the framework of a log-likelihood ratio providing a scoring system based on the likelihood of a sequence given the motif, versus a genomic-frequency based null hypothesis." ;
  rdfs:subClassOf obo:ECO_0000028 .

obo:ECO_0001201 a rdfs:Class ;
  rdfs:label "in vitro polyADP-ribosylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro polyADP-ribosylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001069 .

obo:ECO_0005550 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence based on paralogy evidence" ;
  rdfs:comment "A type of  biological system reconstruction evidence based on homology evidence where the evidence is inferred by paralogy from an existing experimentally supported model to a process, pathway, or complex in the same species." ;
  rdfs:subClassOf obo:ECO_0005549 .

obo:ECO_0001234 a rdfs:Class ;
  rdfs:label "MTS assay evidence used in manual assertion" ;
  rdfs:comment "A type of MTS assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001100 , obo:ECO_0007698 .

obo:ECO_0000002 a rdfs:Class ;
  rdfs:label "direct assay evidence" ;
  rdfs:comment "A type of experimental evidence resulting from the direct measurement of some aspect of a biological feature." ;
  rdfs:subClassOf obo:ECO_0000006 , obo:IAO_0000030 .

obo:ECO_0001149 a rdfs:Class ;
  rdfs:label "cognitive assay phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of cognitive assay phenotypic evidence used that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001012 , obo:ECO_0007634 .

obo:ECO_0006227 a rdfs:Class ;
  rdfs:label "cryogenic electron microscopy-based structural model with high relative B-factor values used in automatic assertion" ;
  rdfs:comment "A type of cryogenic electron microscopy-based structural model with high relative B-factor values that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006190 , obo:ECO_0006209 .

obo:GO_0045760 a rdfs:Class ;
  rdfs:label "positive regulation of action potential" ;
  rdfs:subClassOf obo:GO_0048518 , obo:GO_0065007 , obo:GO_0098900 .

obo:ECO_0008018 a rdfs:Class ;
  rdfs:label "support vector machine evidence used in manual assertion" ;
  rdfs:comment "A type of support vector machine evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008003 , obo:ECO_0008010 .

obo:ECO_0007113 a rdfs:Class ;
  rdfs:label "protein separation followed by fragment identification evidence used in manual assertion" ;
  rdfs:comment "A type of protein separation followed by fragment identification evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000160 , obo:ECO_0006005 .

obo:OBI_0302905 a rdfs:Class ;
  rdfs:label "elution"@en ;
  rdfs:comment "the process of extracting one material from another by washing with a solvent to remove adsorbed material from an adsorbent (as in washing of loaded ion-exchange resins to remove captured ions)"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0007478 a rdfs:Class ;
  rdfs:label "random mutagenesis evidence used in automatic assertion" ;
  rdfs:comment "A type of random mutagenesis phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005529 , obo:ECO_0007325 .

obo:ECO_0000259 a rdfs:Class ;
  rdfs:label "match to InterPro member signature evidence used in automatic assertion" ;
  rdfs:comment "A type of match to InterPro member signature evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000029 , obo:ECO_0000256 .

obo:ECO_0001260 a rdfs:Class ;
  rdfs:label "xenotransplantation phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of xenotransplantation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001137 , obo:ECO_0007634 .

obo:ECO_0005626 a rdfs:Class ;
  rdfs:label "copper-phenanthroline footprinting evidence used in manual assertion" ;
  rdfs:comment "A type of copper-phenanthroline footprinting evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001815 , obo:ECO_0006083 .

obo:ECO_0007424 a rdfs:Class ;
  rdfs:label "in vivo translation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo translation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001088 , obo:ECO_0007799 .

obo:ECO_0006192 a rdfs:Class ;
  rdfs:label "heat capacity-based evidence" ;
  rdfs:comment "A type of direct assay evidence derived from measuring the amount of heat absorbed or released by a sample to monitor chemical reactions, phase transitions or other processes affecting the physical state or chemical composition." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007465 a rdfs:Class ;
  rdfs:label "transgenic organism evidence used in automatic assertion" ;
  rdfs:comment "A type of transgenic organism evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001131 , obo:ECO_0007427 .

obo:GO_1901363 a rdfs:Class ;
  rdfs:label "heterocyclic compound binding" ;
  rdfs:subClassOf obo:GO_0005488 .

obo:ECO_0001555 a rdfs:Class ;
  rdfs:label "palmitoylation assay evidence" ;
  rdfs:comment "A type of direct assay evidence where palmitoylated residues in a protein are detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007017 a rdfs:Class ;
  rdfs:label "photoconversion evidence" ;
  rdfs:comment "A type of fluorescence evidence where a molecule tagged with a fluorescent protein is exposed to ultraviolet or blue light to shift the spectral emission properties for visualization." ;
  rdfs:subClassOf obo:ECO_0001115 .

obo:ECO_0007069 a rdfs:Class ;
  rdfs:label "crystal violet staining evidence" ;
  rdfs:comment "A type of staining evidence that is derived from the use of gentian violet as a general biological stain and an acid-base indicator." ;
  rdfs:subClassOf obo:ECO_0005019 .

obo:ECO_0006389 a rdfs:Class ;
  rdfs:label "iodine-125-labeled ligand binding evidence used in automatic assertion" ;
  rdfs:comment "A type of iodine-125-labeled ligand binding evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006349 , obo:ECO_0007591 .

obo:ECO_0000321 a rdfs:Class ;
  rdfs:label "rapid divergence from ancestral sequence evidence used in manual assertion" ;
  rdfs:comment "A type of rapid divergence from ancestral sequence evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/ird-inferred-rapid-divergence> ;
  rdfs:subClassOf obo:ECO_0000215 , obo:ECO_0000252 .

obo:OBI_0002089 a rdfs:Class ;
  rdfs:label "physical store" ;
  rdfs:comment "A container with an environmental control function." ;
  rdfs:subClassOf obo:OBI_0000967 , obo:OBI_0001034 .

obo:GO_0032077 a rdfs:Class ;
  rdfs:label "positive regulation of deoxyribonuclease activity" ;
  rdfs:subClassOf obo:GO_0032070 , obo:GO_0032075 , obo:GO_0051054 , obo:GO_0065007 .

obo:UBERON_0000477 a rdfs:Class ;
  rdfs:label "anatomical cluster" ;
  rdfs:comment "Anatomical group that has its parts adjacent to one another." ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:ECO_0007705 a rdfs:Class ;
  rdfs:label "obsolete protein assay evidence used in manual assertion" ;
  rdfs:comment "OBSOLETE. A type of protein assay evidence that is used in a manual assertion." .

obo:GO_0032369 a rdfs:Class ;
  rdfs:label "negative regulation of lipid transport" ;
  rdfs:subClassOf obo:GO_0032368 , obo:GO_0051051 , obo:GO_0065007 , obo:GO_1905953 .

obo:OBI_0000967 a rdfs:Class ;
  rdfs:label "container" ;
  rdfs:comment "A device that can be used to restrict the location of material entities over time" ;
  rdfs:subClassOf obo:OBI_0000968 .

obo:GO_1903036 a rdfs:Class ;
  rdfs:label "positive regulation of response to wounding" ;
  rdfs:subClassOf obo:GO_0048584 , obo:GO_0065007 , obo:GO_1903034 .

obo:ECO_0006374 a rdfs:Class ;
  rdfs:label "qualitative macroscopy evidence used in manual assertion" ;
  rdfs:comment "A type of qualitative macroscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001228 , obo:ECO_0006342 .

obo:ECO_0008058 a rdfs:Class ;
  rdfs:label "antibody HB145-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody HB145 to determine that the human MHC class II protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0000096 a rdfs:Class ;
  rdfs:label "electrophoretic mobility shift assay evidence" ;
  rdfs:comment "A type of affinity evidence based on an electrophoretic mobility shift of macromolecules, where proteins, nucleic acids, or both, are combined and the resulting mixtures are electrophoresed under native conditions through polyacrylamide or agarose gel to detect interactions/complexes between proteins and/or nucleic acid." ;
  rdfs:subClassOf obo:ECO_0000021 .

obo:ECO_0006323 a rdfs:Class ;
  rdfs:label "fluorescence microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of fluorescence microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001232 , obo:ECO_0001249 , obo:ECO_0006271 .

obo:ECO_0005644 a rdfs:Class ;
  rdfs:label "immunoprecipitation evidence used in manual assertion" ;
  rdfs:comment "A type of immunoprecipitation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000085 , obo:ECO_0006076 .

obo:OBI_0001480 a rdfs:Class ;
  rdfs:label "phage display library panning" ;
  rdfs:comment """Phage display library panning is a process in which a diverse collection of phages encoding peptides or proteins are screened and the ones encoding active peptides/proteins are selected in an iterative process similar to natural selection.
""" ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:OBI_0001685 a rdfs:Class ;
  rdfs:label "amplification of intermethylated sites assay" ;
  rdfs:comment "An assay that estimates genome-wide DNA methylation and measures methylation of DNA sequences. AIMS is based on the differential enzymatic digestion of genomic DNA with methylation-sensitive and methylation-insensitive isoschizomers followed by restrained PCR amplification of methylated sequences." ;
  rdfs:subClassOf obo:OBI_0000634 .

obo:ECO_0005595 a rdfs:Class ;
  rdfs:label "immunoperoxidase immunolocalization evidence used in manual assertion" ;
  rdfs:comment "A type of immunoperoxidase immunolocalization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005594 , obo:ECO_0006031 .

obo:ECO_0007096 a rdfs:Class ;
  rdfs:label "compositional similarity evidence used in manual assertion" ;
  rdfs:comment "A type of compositional similarity evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000063 , obo:ECO_0000252 .

obo:OBI_0000338 a rdfs:Class ;
  rdfs:label "drawing a conclusion based on data"@en ;
  rdfs:comment "A planned process in which data gathered in an investigation is evaluated in the context of existing knowledge with the objective to generate more general conclusions or to conclude that the data does not allow one to draw general conclusion"@en ;
  rdfs:subClassOf obo:OBI_0000011 .

obo:ECO_0006237 a rdfs:Class ;
  rdfs:label "protein hydrogen-deuterium exchange mass spectrometry evidence used in automatic assertion" ;
  rdfs:comment "A type of protein hydrogen-deuterium exchange mass spectrometry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006195 , obo:ECO_0007433 .

obo:ECO_0007160 a rdfs:Class ;
  rdfs:label "missense mutation pohenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of missense mutation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001247 , obo:ECO_0005506 .

obo:ECO_0006334 a rdfs:Class ;
  rdfs:label "differential scanning fluorimetry evidence" ;
  rdfs:comment "A type of protein thermal shift assay evidence where thermal unfolding is monitored by measuring the fluorescence intensity upon interaction with its environment." ;
  rdfs:subClassOf obo:ECO_0001115 , obo:ECO_0006260 .

obo:ECO_0008127 a rdfs:Class ;
  rdfs:label "antibody HB-164-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody HB-164-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008059 , obo:ECO_0008087 .

obo:GO_0042571 a rdfs:Class ;
  rdfs:label "immunoglobulin complex, circulating" ;
  rdfs:comment "An immunoglobulin complex that is secreted into extracellular space and found in mucosal areas or other tissues or circulating in the blood or lymph. In its canonical form, a circulating immunoglobulin complex is composed of two identical heavy chains and two identical light chains, held together by disulfide bonds. Some forms of are polymers of the basic structure and contain additional components such as J-chain and the secretory component." ;
  rdfs:subClassOf obo:GO_0019814 , obo:OBI_1110022 .

obo:IAO_0000414 a rdfs:Class ;
  rdfs:label "mass measurement datum"@en ;
  rdfs:comment "A scalar measurement datum that is the result of measurement of mass quality"@en ;
  rdfs:subClassOf obo:IAO_0000032 .

obo:IAO_0000004 a rdf:Property ;
  rdfs:label "has measurement value"@en ;
  rdfs:domain obo:IAO_0000032 ;
  rdfs:range xsd:double .

obo:IAO_0000039 a rdf:Property ;
  rdfs:label "has measurement unit label"@en ;
  rdfs:range obo:UO_0000002 , obo:IAO_0000003 ;
  rdfs:subPropertyOf obo:BFO_0000051 .

obo:IAO_0000221 a rdf:Property ;
  rdfs:label "is quality measurement of"@en ;
  rdfs:comment "m is a quality measurement of q at t. When q is a quality, there is a measurement process p that has specified output m, a measurement datum, that is about q"@en ;
  rdfs:domain obo:IAO_0000109 ;
  rdfs:range obo:PATO_0000125 , obo:BFO_0000019 ;
  rdfs:subPropertyOf obo:IAO_0000136 .

obo:GO_0046677 a rdfs:Class ;
  rdfs:label "response to antibiotic" ;
  rdfs:subClassOf obo:GO_0042221 .

obo:ECO_0008000 a rdfs:Class ;
  rdfs:label "machine learning method evidence" ;
  rdfs:comment "A type of computational evidence in which computational systems build models based on sample data, known as training data, in order to make predictions or decisions without being explicitly programmed to do so. Such systems are trained on a combination of existing data about an entity, and are applied to the generation of new labels, such as numerical or textual or categorical." ;
  rdfs:subClassOf obo:ECO_0007672 .

obo:ECO_0007487 a rdfs:Class ;
  rdfs:label "flow cytometry evidence used in automatic assertion" ;
  rdfs:comment "A type of flow cytometry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000268 , obo:ECO_0007307 .

obo:BFO_0000001 a rdfs:Class ;
  rdfs:label "entity"@en .

obo:ECO_0007371 a rdfs:Class ;
  rdfs:label "constitutively active mutant protein evidence used in automatic assertion" ;
  rdfs:comment "A type of constitutively active mutant protein evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001032 , obo:ECO_0007325 .

obo:ECO_0007835 a rdfs:Class ;
  rdfs:label "curator inference from Wikipedia used in automatic assertion" ;
  rdfs:comment "A type of curator inference from Wikipedia that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007638 , obo:ECO_0007834 .

obo:OBI_0001149 a rdfs:Class ;
  rdfs:label "gene knock in"@en ;
  rdfs:comment "a genetic transformation that involves the insertion of a protein coding cDNA sequence at a particular locus in an organism's chromosome. Typically, this is done in mice since the technology for this process is more refined, and because mouse embryonic stem cells are easily manipulated. The difference between knock-in technology and transgenic technology is that a knock-in involves a gene inserted into a specific locus, and is a \"targeted\" insertion."@en ;
  rdfs:subClassOf obo:OBI_0600043 .

obo:ECO_0007728 a rdfs:Class ;
  rdfs:label "anatomical perturbation phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of anatomical perturbation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000240 , obo:ECO_0007634 .

obo:ECO_0006055 a rdfs:Class ;
  rdfs:label "high throughput evidence" ;
  rdfs:comment "A type of evidence where data generation is automated with equipment to allow for assaying samples or molecules in parallel." ;
  rdfs:subClassOf obo:ECO_0000000 .

obo:ECO_0007700 a rdfs:Class ;
  rdfs:label "disk diffusion test evidence used in manual assertion" ;
  rdfs:comment "A type of disk diffusion test evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005605 , obo:ECO_0007084 .

obo:GO_0045901 a rdfs:Class ;
  rdfs:label "positive regulation of translational elongation" ;
  rdfs:subClassOf obo:GO_0006448 , obo:GO_0045727 , obo:GO_0065007 .

obo:GO_0003824 a rdfs:Class ;
  rdfs:label "catalytic activity" ;
  rdfs:comment "Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic." ;
  rdfs:subClassOf obo:GO_0003674 .

obo:ECO_0007456 a rdfs:Class ;
  rdfs:label "radioimmunoassay evidence used in automatic assertion" ;
  rdfs:comment "A type of radioimmunoassay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001121 , obo:ECO_0007819 .

obo:ECO_0006029 a rdfs:Class ;
  rdfs:label "Kadouri drip-fed biofilm assay evidence used in manual assertion" ;
  rdfs:comment "A type of Kadouri drip-fed biofilm assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006021 , obo:ECO_0006028 .

obo:ECO_0008036 a rdfs:Class ;
  rdfs:label "author inference based on intrinsic disorder prediction used in automatic assertion" ;
  rdfs:comment "A type of author inference based on intrinsic disorder prediction that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000213 , obo:ECO_0006217 , obo:ECO_0008031 .

obo:ECO_0007476 a rdfs:Class ;
  rdfs:label "in vivo polyADP-ribosylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo polyADP-ribosylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005500 , obo:ECO_0007797 .

obo:ECO_0007659 a rdfs:Class ;
  rdfs:label "automatically integrated combinatorial experimental evidence used in manual assertion" ;
  rdfs:comment "A type of automatically integrated combinatorial experimental evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000245 , obo:ECO_0007658 .

obo:OBI_0400169 a rdfs:Class ;
  rdfs:label "microscope" ;
  rdfs:comment "A microscope is an instrument which magnifies the view on objects (too small to be viewed by the naked eye) under increased resolution. A microscope can be an optical instrument but also and electronic instrument. There are various kind of optical microscopes, e.g confocal microscope, epifluoresence microscope)" ;
  rdfs:subClassOf obo:OBI_0000398 .

obo:ECO_0005557 a rdfs:Class ;
  rdfs:label "multiple sequence alignment evidence used in automatic assertion" ;
  rdfs:comment "A type of multiple sequence alignment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000248 , obo:ECO_0005555 .

obo:GO_0016788 a rdfs:Class ;
  rdfs:label "hydrolase activity, acting on ester bonds" ;
  rdfs:subClassOf obo:GO_0016787 .

obo:OBI_0000288 a rdfs:Class ;
  rdfs:label "protein-protein interaction detection assay" ;
  rdfs:comment "An assay that determines interactions between proteins, such as protein-protein binding." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007317 a rdfs:Class ;
  rdfs:label "spatial pattern of transcript expression evidence used in automatic assertion" ;
  rdfs:comment "A type of spatial pattern of transcript expression evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000047 , obo:ECO_0007303 .

obo:ECO_0006044 a rdfs:Class ;
  rdfs:label "extracellular recording evidence used in manual assertion" ;
  rdfs:comment "A type of extracellular recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006006 , obo:ECO_0006043 .

obo:OBI_0600038 a rdfs:Class ;
  rdfs:label "addition of molecular label"@en ;
  rdfs:comment "a material processing technique intended to add a molecular label to some input material entity, to allow detection of the molecular target of this label in a detection of molecular label assay"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:GO_2000147 a rdfs:Class ;
  rdfs:label "positive regulation of cell motility" ;
  rdfs:subClassOf obo:GO_0040017 , obo:GO_0051272 , obo:GO_0065007 , obo:GO_2000145 .

obo:ECO_0006363 a rdfs:Class ;
  rdfs:label "intrinsic fluorescence-based protein conformation evidence used in automatic assertion" ;
  rdfs:comment "A type of intrinsic fluorescence-based protein conformation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006336 , obo:ECO_0007450 .

obo:ECO_0007664 a rdfs:Class ;
  rdfs:label "manually integrated combinatorial computational and experimental evidence used in automatic assertion" ;
  rdfs:comment "A type of manually integrated combinatorial computational and experimental evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007662 , obo:ECO_0007676 , obo:ECO_0007829 .

obo:ECO_0006222 a rdfs:Class ;
  rdfs:label "X-ray crystallography-based structural model with high relative B-factor values used in manual assertion" ;
  rdfs:comment "A type of X-ray crystallography-based structural model with high relative B-factor values that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005670 , obo:ECO_0006188 .

obo:ECO_0006283 a rdfs:Class ;
  rdfs:label "electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence used in manual assertion" ;
  rdfs:comment "A type of electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001230 , obo:ECO_0006251 .

obo:ECO_0005584 a rdfs:Class ;
  rdfs:label "macropatch voltage clamp recording evidence" ;
  rdfs:comment "A type of voltage clamp recording evidence where recording is done on a fragment of the cell membrane (attached to or detached from the cell) using a large tip diameter pipette that allows isolating and studying a small number of channels into that fragment (patch)." ;
  rdfs:subClassOf obo:ECO_0005576 .

obo:ECO_0007467 a rdfs:Class ;
  rdfs:label "TACE activity assay evidence used in automatic assertion" ;
  rdfs:comment "A type of TACE activity assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001129 , obo:ECO_0007468 .

obo:OBI_0002444 a rdfs:Class ;
  rdfs:label "measurand role"@en ;
  rdfs:comment "A role borne by a material entity and realized in an assay which achieves the objective to measure the magnitude/concentration/amount of the measurand in the entity bearing evaluant role." ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:ECO_0001142 a rdfs:Class ;
  rdfs:label "[3H]arachidonic acid release assay evidence used in manual assertion" ;
  rdfs:comment "A type of [3H]arachidonic acid release assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001002 , obo:ECO_0007695 .

obo:OBI_0600033 a rdfs:Class ;
  rdfs:label "cell permeabilization"@en ;
  rdfs:comment "A protocol application to permeabilize cell membranes, allowing molecules to more easily pass through the membrane than was possible prior to the protocol application"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0007102 a rdfs:Class ;
  rdfs:label "intermethylated site amplification evidence used in manual assertion" ;
  rdfs:comment "A type of intermethylated site amplification evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000086 , obo:ECO_0001198 .

obo:ECO_0008112 a rdfs:Class ;
  rdfs:label "antibody B8.11.2-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody B8.11.2-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008051 , obo:ECO_0008088 .

obo:GO_0015718 a rdfs:Class ;
  rdfs:label "monocarboxylic acid transport" ;
  rdfs:subClassOf obo:GO_0046942 .

obo:ECO_0001045 a rdfs:Class ;
  rdfs:label "eTag assay evidence" ;
  rdfs:comment "A type of affinity evidence resulting from the quantitative analysis of gene and protein expression monitored by proprietary eTag reporters." ;
  rdfs:subClassOf obo:ECO_0000023 .

obo:OBI_0000043 a rdfs:Class ;
  rdfs:label "xenotransplantation"@en ;
  rdfs:comment "is the transplantation of living cells, tissues or \\norgans from one species to another such as from pigs to humans"@en ;
  rdfs:subClassOf obo:OBI_0000105 .

obo:ECO_0006068 a rdfs:Class ;
  rdfs:label "RNA-sequencing evidence used in manual assertion" ;
  rdfs:comment "A type of high throughput nucleotide sequencing assay evidence used in a manual assertion based on high-throughput (HT) sequencing of fragmented cDNA molecules." ;
  rdfs:subClassOf obo:ECO_0000291 , obo:ECO_0000295 .

obo:ECO_0007108 a rdfs:Class ;
  rdfs:label "beta-glucuronidase fusion protein localization evidence used in manual assertion" ;
  rdfs:comment "A type of beta-glucuronidase fusion protein localization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000130 , obo:ECO_0000314 .

obo:ECO_0007491 a rdfs:Class ;
  rdfs:label "wide-field microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of wide-field microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005588 , obo:ECO_0007435 .

obo:GO_0051094 a rdfs:Class ;
  rdfs:label "positive regulation of developmental process" ;
  rdfs:subClassOf obo:GO_0048518 , obo:GO_0050793 , obo:GO_0065007 .

obo:ECO_0005619 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation- exonuclease evidence used in automatic assertion" ;
  rdfs:comment "A type of chromatin immunoprecipitation- exonuclease evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001806 , obo:ECO_0007786 .

obo:ECO_0008120 a rdfs:Class ;
  rdfs:label "antibody CR11-351-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody CR11-351-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008055 , obo:ECO_0008088 .

obo:ECO_0001066 a rdfs:Class ;
  rdfs:label "in vitro palmitoylation assay evidence" ;
  rdfs:comment "A type of palmitoylation assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001555 .

obo:OBI_0000399 a rdfs:Class ;
  rdfs:label "solid support function"@en ;
  rdfs:comment "A solid support function is a function of a device on which an entity is kept in a defined position and prevented in its movement"@en ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:ECO_0001013 a rdfs:Class ;
  rdfs:label "blocking monoclonal antibody evidence" ;
  rdfs:comment "A type of immunological assay evidence resulting from inhibitory effect of monoclonal antibody combining with an antigen over other antibodies." ;
  rdfs:subClassOf obo:ECO_0000040 .

obo:OBI_0002619 a rdfs:Class ;
  rdfs:label "site-directed mutagenesis" ;
  rdfs:comment "An induced mutation in which a specific base change is programmed into the sequence of a synthetic primer." ;
  rdfs:subClassOf obo:OBI_0001154 .

obo:ECO_0000013 a rdfs:Class ;
  rdfs:label "transgenic rescue experiment evidence" ;
  rdfs:comment "A type of functional complementation evidence resulting from the introduction of a transgene to prevent, or \"rescue\" an organism from a condition." ;
  rdfs:subClassOf obo:ECO_0000012 .

obo:ECO_0008159 a rdfs:Class ;
  rdfs:label "antibody W6/32-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody W6/32-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008075 , obo:ECO_0008087 .

obo:ECO_0007011 a rdfs:Class ;
  rdfs:label "combinatorial experimental and author inference evidence" ;
  rdfs:comment "A type of combinatorial evidence in which the author draws a conclusion based on a combination of prior scientific background knowledge and evidence from one or more experiments." ;
  rdfs:subClassOf obo:ECO_0000212 .

obo:ECO_0007761 a rdfs:Class ;
  rdfs:label "curator inference from MedlinePlus dictionary used in manual assertion" ;
  rdfs:comment "A type of curator inference from MedlinePlus dictionary that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007644 , obo:ECO_0007760 .

obo:OBI_0000860 a rdfs:Class ;
  rdfs:label "northern blot assay" ;
  rdfs:comment "An assay that determines the presence of gene transcripts by hybridizing labeled RNA or DNA probes against messenger RNAs isolated from tissue or cell cultures, resolved on denaturing gel, transfered by a blotting procedure to a solid support. Detection of hybridization signals is carried out by immunofluorescence or radioactivity measurements using photographic films or digital imaging devices such as Phosphor Imager." ;
  rdfs:subClassOf obo:OBI_0000443 .

obo:GO_0031099 a rdfs:Class ;
  rdfs:label "regeneration" ;
  rdfs:subClassOf obo:GO_0048856 .

obo:OBI_0000845 a rdfs:Class ;
  rdfs:label "primary structure of RNA molecule" ;
  rdfs:comment "The primary structure of an RNA molecule that is completely defined by the set of its nucleic residue parts and the linear order induced by the peptide bonds that hold them together" ;
  rdfs:subClassOf obo:SO_0000001 .

obo:ECO_0005547 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005548 , obo:ECO_0007746 .

obo:GO_0032413 a rdfs:Class ;
  rdfs:label "negative regulation of ion transmembrane transporter activity" ;
  rdfs:subClassOf obo:GO_0032410 , obo:GO_0032412 , obo:GO_0034766 , obo:GO_0065007 .

obo:OBI_0000535 a rdfs:Class ;
  rdfs:label "gel filtration column" ;
  rdfs:comment "A Gel filtration column is a chromatography column for size-exclusion chromatography, in which the stationary phase is a gel. The main application of gel filtration chromatography is the fractionation of proteins and other water-soluble polymers." ;
  rdfs:subClassOf obo:OBI_0000618 .

obo:ECO_0008061 a rdfs:Class ;
  rdfs:label "antibody ILA-21-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody ILA-21 to determine that the cattle MHC class II protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0007627 a rdfs:Class ;
  rdfs:label "colony size phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of colony size phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007081 , obo:ECO_0007625 .

obo:ECO_0007548 a rdfs:Class ;
  rdfs:label "fluorescent sensor evidence used in automatic assertion" ;
  rdfs:comment "A type of fluorescent sensor evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006034 , obo:ECO_0007450 .

obo:ECO_0001561 a rdfs:Class ;
  rdfs:label "translation assay evidence" ;
  rdfs:comment "A type of direct assay evidence where de novo protein synthesis is detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0000337 a rdfs:Class ;
  rdfs:label "gel electrophoresis evidence" ;
  rdfs:comment "A type of direct assay evidence where molecules have been sorted according to their size and charge by moving through a gel in the presence of an electric field." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:GO_0034762 a rdfs:Class ;
  rdfs:label "regulation of transmembrane transport" ;
  rdfs:subClassOf obo:GO_0050794 , obo:GO_0051049 , obo:GO_0065007 .

obo:ECO_0001056 a rdfs:Class ;
  rdfs:label "obsolete induced mutation evidence" ;
  rdfs:comment "OBSOLETE. A type of genetic transformation evidence resulting from a mutation induced by a mutagenic compounds or irradiation." .

obo:OBI_0001403 a rdfs:Class ;
  rdfs:label "ChIP-chip design" ;
  rdfs:comment "A study design which aims to identify protein binding sites in genomic DNA by a combination of chromatin immunoprecipitation and DNA microarray (chip) assays." ;
  rdfs:subClassOf obo:OBI_0001425 .

obo:ECO_0005558 a rdfs:Class ;
  rdfs:label "motif discovery evidence used in manual assertion" ;
  rdfs:comment "A type of motif discovery evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005531 , obo:ECO_0005556 .

obo:ECO_0007331 a rdfs:Class ;
  rdfs:label "rapid divergence from ancestral sequence evidence used in automatic assertion" ;
  rdfs:comment "A type of rapid divergence from ancestral sequence evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000215 , obo:ECO_0007826 .

obo:ECO_0007717 a rdfs:Class ;
  rdfs:label "translation assay evidence used in manual assertion" ;
  rdfs:comment "A type of translation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001561 .

obo:GO_0031343 a rdfs:Class ;
  rdfs:label "positive regulation of cell killing" ;
  rdfs:subClassOf obo:GO_0031341 , obo:GO_0048522 , obo:GO_0065007 .

obo:ECO_0005663 a rdfs:Class ;
  rdfs:label "regular expression motif search evidence used in automatic assertion" ;
  rdfs:comment "A type of regular expression motif search evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000258 , obo:ECO_0005505 .

obo:ECO_0000114 a rdfs:Class ;
  rdfs:label "expression library screen evidence" ;
  rdfs:comment "A type of protein expression evidence where a protein of interest is isolated on the basis of its hybridization to an antibody raised to a homologous protein." ;
  rdfs:subClassOf obo:ECO_0000010 .

obo:OBI_0000435 a rdfs:Class ;
  rdfs:label "genotyping assay" ;
  rdfs:comment "An assay which generates data about a genotype from a specimen of genomic DNA. A variety of techniques and instruments can be used to produce information about sequence variation at particular genomic positions." ;
  rdfs:subClassOf obo:OBI_0000443 .

obo:ECO_0001603 a rdfs:Class ;
  rdfs:label "cell staining evidence" ;
  rdfs:comment "A type of cell-based assay evidence in which living cells are stained with a vital stain, i.e. dye that can be used on living cells without causing cell death." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:ECO_0001810 a rdfs:Class ;
  rdfs:label "DNAse footprinting evidence" ;
  rdfs:comment "A type of nucleic acid binding evidence where proteins that have been bound to DNA protect a binding site from enzymatic cleavage with DNAse, thereby detecting protein-DNA interactions." ;
  rdfs:subClassOf obo:ECO_0000136 .

obo:ECO_0001208 a rdfs:Class ;
  rdfs:label "in vivo protein cleavage assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo protein cleavage assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001075 .

obo:ECO_0007692 a rdfs:Class ;
  rdfs:label "deacetylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of deacetylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001548 .

obo:ECO_0006079 a rdfs:Class ;
  rdfs:label "yeast one-hybrid evidence used in manual assertion" ;
  rdfs:comment "A type of hybrid interaction evidence that is used in manual assertion that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors." ;
  rdfs:subClassOf obo:ECO_0000066 , obo:ECO_0006077 .

obo:OBI_0000978 a rdfs:Class ;
  rdfs:label "in container assay" ;
  rdfs:comment "An assay in which a measurement is made by observing entities located in a container." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007488 a rdfs:Class ;
  rdfs:label "enzyme-linked immunoabsorbent assay evidence used in automatic assertion" ;
  rdfs:comment "A type of enzyme-linked immunoabsorbent assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000267 , obo:ECO_0007819 .

obo:ECO_0006262 a rdfs:Class ;
  rdfs:label "native protein gel electrophoresis evidence" ;
  rdfs:comment "A type of gel electrophoresis evidence resulting from the use of non-denaturing conditions so that the proteins being measured are in their native structural states." ;
  rdfs:subClassOf obo:ECO_0000337 .

obo:ECO_0001130 a rdfs:Class ;
  rdfs:label "tissue microarray evidence" ;
  rdfs:comment "A type of cytochemistry evidence resulting from the analysis of section(s) of a paraffin block containing embedded tissues cores arranged in an array pattern." ;
  rdfs:subClassOf obo:ECO_0001035 .

obo:ECO_0001168 a rdfs:Class ;
  rdfs:label "conditional knockout evidence used in manual assertion" ;
  rdfs:comment "A type of conditional knockout evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001030 , obo:ECO_0001225 .

obo:ECO_0008047 a rdfs:Class ;
  rdfs:label "antibody AG2.42.7-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody AG2.42.7 to determine that the H2-IAg7 protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0007801 a rdfs:Class ;
  rdfs:label "ubiquitination assay evidence used in automatic assertion" ;
  rdfs:comment "A type of ubiquitination assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001562 , obo:ECO_0007307 .

obo:ECO_0006328 a rdfs:Class ;
  rdfs:label "dynamic fluorescence quenching evidence used in automatic assertion" ;
  rdfs:comment "A type of dynamic fluorescence quenching evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006273 , obo:ECO_0007332 , obo:ECO_0007450 .

obo:ECO_0000049 a rdfs:Class ;
  rdfs:label "reporter gene assay evidence" ;
  rdfs:comment "A type of expression pattern evidence that is based on the expression pattern of a reporter gene." ;
  rdfs:subClassOf obo:ECO_0000008 , obo:IAO_0000030 .

obo:OBI_0200085 a rdfs:Class ;
  rdfs:label "mass spectrometry analysis"@en ;
  rdfs:comment "A data transformation which has the objective of spectrum analysis." ;
  rdfs:subClassOf obo:OBI_0200000 .

obo:ECO_0001034 a rdfs:Class ;
  rdfs:label "crystallography evidence" ;
  rdfs:comment "A type of structure determination evidence resulting from the use of a narrow beam of x-rays to identify molecular structures by creating a diffraction pattern." ;
  rdfs:subClassOf obo:ECO_0005031 .

obo:ECO_0007120 a rdfs:Class ;
  rdfs:label "animal model system study evidence used in manual assertion" ;
  rdfs:comment "A type of animal model system study evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000179 , obo:ECO_0000269 .

obo:OBI_0002082 a rdfs:Class ;
  rdfs:label "reporter gene assay" ;
  rdfs:comment "An assay that detects expression of a reporter gene that was inserted under the control of a regulatory sequence of interest." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:OBI_0000374 a rdfs:Class ;
  rdfs:label "excitation function"@en ;
  rdfs:comment "A excitation function is a function  to inject energy by bombarding a material with energetic particles (e.g., photons) thereby imbuing internal material components such as electrons with additional energy.  These internal, 'excited' particles may lead to the rupturing of covalent chemical bonds or may quickly relax back to there unexcited state with an exponential time course thereby locally emitting energy in the form of photons."@en ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:ECO_0007001 a rdfs:Class ;
  rdfs:label "high throughput mutant phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of high throughput mutant phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0006056 , obo:ECO_0007000 .

obo:OBI_0002175 a rdfs:Class ;
  rdfs:label "ATP bioluminescence assay" ;
  rdfs:comment "An analyte assay that detects ATP concentration through light intensity when luciferase catalyzes the oxidation of luciferin in the presence of ATP, magnesium ions, and molecular oxygen." ;
  rdfs:subClassOf obo:OBI_0000443 .

obo:ECO_0007797 a rdfs:Class ;
  rdfs:label "polyADP-ribosylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of polyADP-ribosylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001557 , obo:ECO_0007307 .

obo:ECO_0001572 a rdfs:Class ;
  rdfs:label "colony diameter phenotype evidence used in automatic assertion" ;
  rdfs:comment "A type of colony diameter phenotype evidence that is used in automatic assertion" ;
  rdfs:subClassOf obo:ECO_0001571 , obo:ECO_0007627 .

obo:ECO_0008138 a rdfs:Class ;
  rdfs:label "antibody L243-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody L243-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008064 , obo:ECO_0008088 .

obo:ECO_0001146 a rdfs:Class ;
  rdfs:label "allograft transplantation phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of allograft transplantation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001009 , obo:ECO_0007634 .

obo:ECO_0001558 a rdfs:Class ;
  rdfs:label "protein kinase assay evidence" ;
  rdfs:comment "A type of enzymatic activity assay evidence that measures transfer of a phosphate to a peptide or protein substrate by a protein kinase." ;
  rdfs:subClassOf obo:ECO_0000005 .

obo:GO_0097367 a rdfs:Class ;
  rdfs:label "carbohydrate derivative binding" ;
  rdfs:subClassOf obo:GO_0005488 .

obo:OBI_0001674 a rdfs:Class ;
  rdfs:label "cap analysis of gene expression assay" ;
  rdfs:comment "A transcription profiling assay which measures RNA transcript abundances in biological samples by extracting 5' ends of capped transcripts, RTPCR and sequence those. Copy numbers of CAGE tags provide a way of quantification and provide a measure of expression of the transcriptome" ;
  rdfs:subClassOf obo:OBI_0000424 , obo:OBI_0002692 , obo:OBI_0600047 .

obo:ECO_0007653 a rdfs:Class ;
  rdfs:label "automatically integrated combinatorial computational evidence used in automatic assertion" ;
  rdfs:comment "A type of automatically integrated combinatorial computational evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000053 , obo:ECO_0007651 , obo:ECO_0007679 .

obo:ECO_0001219 a rdfs:Class ;
  rdfs:label "in vivo sumoylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo sumoylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001086 .

obo:GO_0070549 a rdfs:Class ;
  rdfs:label "siRNA-mediated gene silencing by inhibition of translation" ;
  rdfs:subClassOf obo:GO_0040033 .

obo:ECO_0001588 a rdfs:Class ;
  rdfs:label "ion trap mass spectrometry evidence used in manual assertion" ;
  rdfs:comment "A type of ion trap mass spectrometry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001230 , obo:ECO_0001586 .

obo:OBI_0000424 a rdfs:Class ;
  rdfs:label "transcription profiling assay" ;
  rdfs:comment "An assay that determines gene expression and transcription activity using ribonucleic acids collected from a material entity." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:IAO_0000007 a rdfs:Class ;
  rdfs:label "action specification"@en ;
  rdfs:comment "A directive information entity that describes an action the bearer will take."@en ;
  rdfs:subClassOf obo:IAO_0000033 .

obo:ECO_0005652 a rdfs:Class ;
  rdfs:label "methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in manual assertion" ;
  rdfs:comment "A type of methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001814 , obo:ECO_0006083 .

obo:GO_0060548 a rdfs:Class ;
  rdfs:label "negative regulation of cell death" ;
  rdfs:subClassOf obo:GO_0010941 , obo:GO_0048523 , obo:GO_0065007 .

obo:OBI_0000744 a rdfs:Class ;
  rdfs:label "material sampling process" ;
  rdfs:comment "A specimen gathering process with the objective to obtain a specimen that is representative of the input material entity" ;
  rdfs:subClassOf obo:OBI_0000659 .

obo:GO_0051128 a rdfs:Class ;
  rdfs:label "regulation of cellular component organization" ;
  rdfs:subClassOf obo:GO_0050794 , obo:GO_0065007 .

obo:GO_0009057 a rdfs:Class ;
  rdfs:label "macromolecule catabolic process" ;
  rdfs:subClassOf obo:GO_0043170 , obo:GO_1901575 .

obo:GO_0030336 a rdfs:Class ;
  rdfs:label "negative regulation of cell migration" ;
  rdfs:subClassOf obo:GO_0030334 , obo:GO_0065007 , obo:GO_2000146 .

obo:ECO_0006293 a rdfs:Class ;
  rdfs:label "protein fragment functional complementation evidence used in manual assertion" ;
  rdfs:comment "A type of protein fragment functional complementation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000353 , obo:ECO_0006256 .

obo:ECO_0007278 a rdfs:Class ;
  rdfs:label "current clamp recording evidence used in automatic assertion" ;
  rdfs:comment "A type of current clamp recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005571 , obo:ECO_0007535 .

obo:ECO_0007605 a rdfs:Class ;
  rdfs:label "5-cyano-2,3-ditolyl tetrazolium chloride staining evidence used in automatic assertion" ;
  rdfs:comment "A type of 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007037 , obo:ECO_0007798 .

obo:ECO_0006077 a rdfs:Class ;
  rdfs:label "bait-prey hybrid interaction evidence used in manual assertion" ;
  rdfs:comment "A type of bait-prey hybrid interaction evidence that is used in manual assertion where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." ;
  rdfs:subClassOf obo:ECO_0000025 , obo:ECO_0006392 .

obo:ECO_0001599 a rdfs:Class ;
  rdfs:label "multilocus sequence typing evidence used in automatic assertion" ;
  rdfs:comment "A type of multilocus sequence typing evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001598 , obo:ECO_0007813 .

obo:OBI_0001248 a rdfs:Class ;
  rdfs:label "ChIP-chip assay" ;
  rdfs:comment "An assay in which chromatin is immunoprecipitated and subsequently analyzed using a DNA microarray to identify which parts of DNA are part of the isolated chromatin" ;
  rdfs:subClassOf obo:OBI_0001954 .

obo:ECO_0007070 a rdfs:Class ;
  rdfs:label "crystal violet staining evidence used in manual assertion" ;
  rdfs:comment "A type of crystal violet staining evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0007069 .

obo:ECO_0007436 a rdfs:Class ;
  rdfs:label "motility wound healing assay evidence used in automatic assertion" ;
  rdfs:comment "A type of motility wound healing assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001099 , obo:ECO_0007773 .

obo:ECO_0001157 a rdfs:Class ;
  rdfs:label "cell counting evidence used in manual assertion" ;
  rdfs:comment "A type of cell counting evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001020 , obo:ECO_0007695 .

obo:ECO_0005676 a rdfs:Class ;
  rdfs:label "ad-hoc quantitative phenotype observation evidence used in manual assertion" ;
  rdfs:comment "A type of ad-hoc quantitative phenotype observation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005675 , obo:ECO_0007634 .

obo:ECO_0005591 a rdfs:Class ;
  rdfs:label "immunogold labelling electron microscopy assay evidence" ;
  rdfs:comment "A type of immunogold labelling evidence where electron microscopy technique is used to detect the location of the antibodies labeled with colloidal gold particles." ;
  rdfs:subClassOf obo:ECO_0000090 , obo:ECO_0005033 .

obo:OBI_0002116 a rdfs:Class ;
  rdfs:label "high performance liquid chromotography assay" ;
  rdfs:comment "An analytical chromatography assay that utilizes a high performance liquid chromatography instrument for separation of compounts in a solution." ;
  rdfs:subClassOf obo:OBI_0001630 .

obo:ECO_0007289 a rdfs:Class ;
  rdfs:label "cross-streak test evidence used in automatic assertion" ;
  rdfs:comment "A type of cross-streak test evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005604 , obo:ECO_0007775 .

obo:ECO_0008011 a rdfs:Class ;
  rdfs:label "string-matching method evidence" ;
  rdfs:comment "A type of computational evidence that employs a string algorithm to identify a string within a larger string or body of text, where a string is a particular series of characters consisting of letters, numbers, symbols, spaces, etc." ;
  rdfs:subClassOf obo:ECO_0007672 .

obo:GO_0006414 a rdfs:Class ;
  rdfs:label "translational elongation" ;
  rdfs:subClassOf obo:GO_0034645 .

obo:ECO_0007417 a rdfs:Class ;
  rdfs:label "in vivo farnesylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo farnesylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001081 , obo:ECO_0007800 .

obo:GO_0015849 a rdfs:Class ;
  rdfs:label "organic acid transport" ;
  rdfs:subClassOf obo:GO_0071702 .

obo:ECO_0007382 a rdfs:Class ;
  rdfs:label "dominant-negative mutant phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of dominant-negative mutant phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001042 , obo:ECO_0007325 .

obo:GO_0097159 a rdfs:Class ;
  rdfs:label "organic cyclic compound binding" ;
  rdfs:subClassOf obo:GO_0005488 .

obo:ECO_0006406 a rdfs:Class ;
  rdfs:label "experimentally evolved mutant phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of experimentally evolved mutant phenotyic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0006404 .

obo:ECO_0007428 a rdfs:Class ;
  rdfs:label "lipid binding assay evidence used in automatic assertion" ;
  rdfs:comment "A type of lipid binding assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001092 , obo:ECO_0007332 .

obo:ECO_0006020 a rdfs:Class ;
  rdfs:label "biofilm formation assay evidence" ;
  rdfs:comment "A type of cell proliferation assay evidence in which biofilm growth is monitored and detected from attachment to development." ;
  rdfs:subClassOf obo:ECO_0005007 .

obo:ECO_0007610 a rdfs:Class ;
  rdfs:label "time-lapsed microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of time-lapsed microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007047 , obo:ECO_0007435 .

obo:OBI_0001700 a rdfs:Class ;
  rdfs:label "immunoprecipitation assay" ;
  rdfs:comment "An analyte assay in which an input material is mixed with antibodies and bound antigen:antibody complexes are separated out using immunoprecipitation. Either the antibody has known specificy, and the antigen mixture is tested for the presence of a specific antigen, or the antigen solution is well defined and the antibody solution is tested for the presence of antigen specific antibodies." ;
  rdfs:subClassOf obo:OBI_0000443 .

obo:ECO_0007750 a rdfs:Class ;
  rdfs:label "phylogenetic evidence used in manual assertion" ;
  rdfs:comment "A type of phylogenetic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000080 , obo:ECO_0000252 .

obo:ECO_0008072 a rdfs:Class ;
  rdfs:label "antibody SF1-1.1.1-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody SF1-1.1.1 to determine that the H2-Kd protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:OBI_0001479 a rdfs:Class ;
  rdfs:label "specimen from organism"@en ;
  rdfs:comment "A specimen that derives from an anatomical part or substance arising from an organism. Examples of tissue specimen include tissue, organ, physiological system, blood, or body location (arm)." ;
  rdfs:subClassOf obo:OBI_0100051 .

obo:ECO_0000229 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation-seq evidence" ;
  rdfs:comment "A type of chromatin immunoprecipitation evidence that uses high-throughput sequencing where immunoprecipitated DNA fragments are funnelled into a massively parallel sequencer to produce multiple short reads for locating binding sites of DNA-associated proteins." ;
  rdfs:subClassOf obo:ECO_0000226 .

obo:BFO_0000004 a rdfs:Class ;
  rdfs:label "independent continuant"@en ;
  rdfs:comment "A continuant that is a bearer of quality and realizable entity entities, in which other entities inhere and which itself cannot inhere in anything."@en ;
  rdfs:subClassOf obo:BFO_0000002 .

obo:GO_0034654 a rdfs:Class ;
  rdfs:label "nucleobase-containing compound biosynthetic process" ;
  rdfs:subClassOf obo:GO_0006139 , obo:GO_0018130 , obo:GO_0019438 , obo:GO_0044271 , obo:GO_1901362 .

obo:ECO_0006062 a rdfs:Class ;
  rdfs:label "wide-field fluorescence microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of wide-field microscopy evidence that is used in a manual assertion where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter." ;
  rdfs:subClassOf obo:ECO_0005590 , obo:ECO_0005598 .

obo:OBI_0001909 a rdfs:Class ;
  rdfs:label "conclusion based on data" ;
  rdfs:comment "An information content entity that is inferred from data." ;
  rdfs:subClassOf obo:IAO_0000030 .

obo:OBI_0000124 a rdf:Property ;
  rdfs:label "is_supported_by_data" ;
  rdfs:comment "The relation between a data item and a conclusion where the conclusion is the output of a data interpreting process and the data item is used as an input to that process" ;
  rdfs:domain obo:OBI_0001909 ;
  rdfs:range obo:IAO_0000027 .

obo:ECO_0007300 a rdfs:Class ;
  rdfs:label "cRNA to DNA expression microarray evidence used in automatic assertion" ;
  rdfs:comment "A type of cRNA to DNA expression microarray evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000062 , obo:ECO_0007301 .

obo:ECO_0007081 a rdfs:Class ;
  rdfs:label "colony size phenotypic evidence" ;
  rdfs:comment "A type of colony morphology phenotypic evidence resulting from measuring the area or diameter of the colony." ;
  rdfs:subClassOf obo:ECO_0007077 .

obo:GO_1903793 a rdfs:Class ;
  rdfs:label "positive regulation of anion transport" ;
  rdfs:subClassOf obo:GO_0043270 , obo:GO_0044070 , obo:GO_0065007 .

obo:ECO_0008101 a rdfs:Class ;
  rdfs:label "antibody 4B7/8-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody 4B7/8-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008046 , obo:ECO_0008087 .

obo:OBI_0000423 a rdfs:Class ;
  rdfs:label "extract"@en ;
  rdfs:comment "an extract is a material entity which results from an extraction process"@en ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:GO_0032268 a rdfs:Class ;
  rdfs:label "regulation of cellular protein metabolic process" ;
  rdfs:subClassOf obo:GO_0031323 , obo:GO_0051246 , obo:GO_0065007 .

obo:ECO_0007515 a rdfs:Class ;
  rdfs:label "isothermal titration calorimetry evidence used in automatic assertion" ;
  rdfs:comment "A type of isothermal titration calorimetry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001825 , obo:ECO_0007384 .

obo:ECO_0005622 a rdfs:Class ;
  rdfs:label "comparative genomics motif search evidence used in manual assertion" ;
  rdfs:comment "A type of comparative genomics motif search evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000257 , obo:ECO_0005534 .

obo:ECO_0000240 a rdfs:Class ;
  rdfs:label "anatomical perturbation phenotypic evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence resulting from the disruption of a structural feature." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0006352 a rdfs:Class ;
  rdfs:label "Congo red staining evidence used in manual assertion" ;
  rdfs:comment "A type of Congo red staining evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0006331 .

obo:OBI_0000736 a rdfs:Class ;
  rdfs:label "single fragment library" ;
  rdfs:comment "is a collection of short tags from DNA fragments, are extracted and covalently linked as single tag constructs" ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:ECO_0001083 a rdfs:Class ;
  rdfs:label "in vivo palmitoylation assay evidence" ;
  rdfs:comment "A type of palmitoylation assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001555 .

obo:GO_1901360 a rdfs:Class ;
  rdfs:label "organic cyclic compound metabolic process" ;
  rdfs:subClassOf obo:GO_0071704 .

obo:GO_0044089 a rdfs:Class ;
  rdfs:label "positive regulation of cellular component biogenesis" ;
  rdfs:subClassOf obo:GO_0044087 , obo:GO_0048522 , obo:GO_0065007 .

obo:OBI_1110057 a rdfs:Class ;
  rdfs:label "MHC:epitope complex binding to TCR"@en ;
  rdfs:comment "a process of an MHC molecule binding to an entity playing the role of epitope to create an MHC:epitope complex which is then bound by a TCR molecule."@en ;
  rdfs:subClassOf obo:OBI_0001588 , obo:OBI_1110014 .

obo:ECO_0007780 a rdfs:Class ;
  rdfs:label "chemotaxis assay evidence used in automatic assertion" ;
  rdfs:comment "A type of chemotaxis assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005021 , obo:ECO_0007773 .

obo:ECO_0007690 a rdfs:Class ;
  rdfs:label "ex vivo assay evidence used in manual assertion" ;
  rdfs:comment "A type of ex vivo assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0005502 .

obo:BFO_0000015 a rdfs:Class ;
  rdfs:label "process"@en ;
  rdfs:comment "An occurrent that has temporal proper parts and for some time t, p s-depends_on some material entity at t."@en ;
  rdfs:subClassOf obo:BFO_0000003 .

obo:GO_0004520 a rdfs:Class ;
  rdfs:label "endodeoxyribonuclease activity" ;
  rdfs:subClassOf obo:GO_0004519 , obo:GO_0004536 .

obo:OBI_0000454 a rdfs:Class ;
  rdfs:label "extracellular electrophysiology recording assay" ;
  rdfs:comment "An electrophysiology recording assay where the recording location of the electrode is extracellular" ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:OBI_0001979 a rdfs:Class ;
  rdfs:label "fluorescence quenching binding assay" ;
  rdfs:comment "A binding assay in which the proximity of two entities is monitored by measuring a fluorescent signal of one of the entities that gets reduced if the two entities are cliose to each other." ;
  rdfs:subClassOf obo:OBI_0001146 .

obo:ECO_0006031 a rdfs:Class ;
  rdfs:label "immunolocalization evidence used in manual assertion" ;
  rdfs:comment "A type of immunolocalization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000087 , obo:ECO_0001232 .

obo:OBI_0400147 a rdfs:Class ;
  rdfs:label "microarray" ;
  rdfs:comment "A processed material that is made to be used in an analyte assay. It consists of a physical immobilisation matrix in which substances that bind the analyte are placed in regular spatial position." ;
  rdfs:subClassOf obo:OBI_0001865 .

obo:ECO_0001229 a rdfs:Class ;
  rdfs:label "mammalian 2-hybrid assay evidence used in manual assertion" ;
  rdfs:comment "A type of mammalian 2-hybrid assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001095 , obo:ECO_0006077 .

obo:OBI_0302859 a rdfs:Class ;
  rdfs:label "genetically modified organism"@en ;
  rdfs:comment "an organism that is the output of a genetic transformation process"@en ;
  rdfs:subClassOf obo:OBI_0001151 .

obo:ECO_0005573 a rdfs:Class ;
  rdfs:label "cell-attached single-channel recording evidence" ;
  rdfs:comment "A type of patch-clamp recording evidence that involves isolation of a patch of membrane electrically from the external solution and to record current flowing into the patch and this is achieved by pressing a fire-polished glass pipette, which has been filled with a suitable electrolyte solution, against the surface of a cell and applying light suction." ;
  rdfs:subClassOf obo:ECO_0006012 .

obo:ECO_0001077 a rdfs:Class ;
  rdfs:label "in vivo defarnesylation assay evidence" ;
  rdfs:comment "A type of defarnesylation assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001549 .

obo:ECO_0005629 a rdfs:Class ;
  rdfs:label "DNA affinity chromatography evidence used in manual assertion" ;
  rdfs:comment "A type of DNA affinity chromatography evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001809 , obo:ECO_0007680 .

obo:ECO_0007477 a rdfs:Class ;
  rdfs:label "DNA dot blot assay evidence used in automatic assertion" ;
  rdfs:comment "A type of DNA dot blot assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005522 , obo:ECO_0007817 .

obo:GO_0060196 a rdfs:Class ;
  rdfs:label "positive regulation of antisense RNA transcription" ;
  rdfs:subClassOf obo:GO_0045893 , obo:GO_0060194 , obo:GO_0065007 .

obo:GO_0043565 a rdfs:Class ;
  rdfs:label "sequence-specific DNA binding" ;
  rdfs:comment "Interacting selectively and non-covalently with DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA e.g. promotor binding or rDNA binding." ;
  rdfs:subClassOf obo:GO_0003677 , obo:OBI_0001588 .

obo:ECO_0007469 a rdfs:Class ;
  rdfs:label "surface plasmon resonance evidence used in automatic assertion" ;
  rdfs:comment "A type of surface plasmon resonance evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001127 , obo:ECO_0007332 .

obo:ECO_0005606 a rdfs:Class ;
  rdfs:label "cell transfection experiment evidence" ;
  rdfs:comment "A type of experimental evidence where a foreign genetic material is introduced into the cell for study of gene function and regulation and protein function." ;
  rdfs:subClassOf obo:ECO_0000006 , obo:IAO_0000030 .

obo:GO_0043414 a rdfs:Class ;
  rdfs:label "macromolecule methylation" ;
  rdfs:subClassOf obo:GO_0032259 , obo:GO_0043412 , obo:GO_0044260 .

obo:ECO_0005633 a rdfs:Class ;
  rdfs:label "ferric uptake regulator titration assay evidence used in manual assertion" ;
  rdfs:comment "A type of ferric uptake regulator titration assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000270 , obo:ECO_0001829 .

obo:ECO_0006294 a rdfs:Class ;
  rdfs:label "protein fragment functional complementation evidence used in automatic assertion" ;
  rdfs:comment "A type of protein fragment functional complementation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006256 , obo:ECO_0007332 .

obo:ECO_0007225 a rdfs:Class ;
  rdfs:label "clinical study evidence used in automatic assertion" ;
  rdfs:comment "A type of clinical study evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000180 , obo:ECO_0007297 .

obo:ECO_0006316 a rdfs:Class ;
  rdfs:label "pH-induced protein unfolding evidence used in automatic assertion" ;
  rdfs:comment "A type of pH-induced protein unfolding evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006267 , obo:ECO_0006312 .

obo:ECO_0000275 a rdfs:Class ;
  rdfs:label "expression microarray evidence used in manual assertion" ;
  rdfs:comment "A type of expression microarray evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000058 , obo:ECO_0000291 .

obo:OBI_0600053 a rdfs:Class ;
  rdfs:label "electrophoresis"@en ;
  rdfs:comment "a protocol application that uses an electrical potential to move material through a defined matrix in order to separate it by its resistance to movement and its charge"@en ;
  rdfs:subClassOf obo:OBI_0600014 .

obo:GO_0045927 a rdfs:Class ;
  rdfs:label "positive regulation of growth" ;
  rdfs:subClassOf obo:GO_0040008 , obo:GO_0048518 , obo:GO_0065007 .

obo:ECO_0007846 a rdfs:Class ;
  rdfs:label "radioisotope quantification assay evidence used in manual assertion" ;
  rdfs:comment "A type of radioisotope quantification assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001254 , obo:ECO_0007844 .

obo:GO_0006276 a rdfs:Class ;
  rdfs:label "plasmid maintenance" ;
  rdfs:subClassOf obo:GO_0009987 .

obo:ECO_0007171 a rdfs:Class ;
  rdfs:label "current density evidence used in manual assertion" ;
  rdfs:comment "A type of current density evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005564 , obo:ECO_0006006 .

obo:ECO_0008148 a rdfs:Class ;
  rdfs:label "antibody MKD6-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody MKD6-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008069 , obo:ECO_0008088 .

obo:GO_0032880 a rdfs:Class ;
  rdfs:label "regulation of protein localization" ;
  rdfs:subClassOf obo:GO_0032879 , obo:GO_0065007 .

obo:GO_0006810 a rdfs:Class ;
  rdfs:label "transport" ;
  rdfs:subClassOf obo:GO_0051234 .

obo:ECO_0007677 a rdfs:Class ;
  rdfs:label "combinatorial computational evidence" ;
  rdfs:comment "A type of combinatorial evidence in which at least two distinct types of computational evidence have been integrated." ;
  rdfs:subClassOf obo:ECO_0000212 .

obo:OBI_0001689 a rdfs:Class ;
  rdfs:label "cytochalasin-induced inhibition of actin polymerization assay" ;
  rdfs:comment "An assay which uses compound cytochalasin (CHEBI: 23528) to block actin polymerization-dependent cell motility (GO:0070358) and actin filament polymerization (GO:0030041)." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007283 a rdfs:Class ;
  rdfs:label "electroencephalography recording evidence used in automatic assertion" ;
  rdfs:comment "A type of electroencephalography recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005577 , obo:ECO_0007535 .

obo:ECO_0007220 a rdfs:Class ;
  rdfs:label "biochemical trait analysis evidence used in automatic assertion" ;
  rdfs:comment "A type of biochemical trait analysis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000172 , obo:ECO_0007635 .

obo:ECO_0006143 a rdfs:Class ;
  rdfs:label "quantum mechanics simulation evidence used in manual assertion" ;
  rdfs:comment "A type of quantum mechanics simulation evidence that is used in manual assertion." ;
  rdfs:subClassOf obo:ECO_0006141 .

obo:ECO_0007812 a rdfs:Class ;
  rdfs:label "sequencing assay evidence used in automatic assertion" ;
  rdfs:comment "A type of sequencing assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000220 , obo:ECO_0007297 .

obo:ECO_0001803 a rdfs:Class ;
  rdfs:label "chloramphenicol acetyltransferase reporter gene assay evidence" ;
  rdfs:comment "A type of reporter gene assay evidence based on the fusion of the CAT gene to a specific promoter for the expression of chloramphenicol acetyltransferase which confers resistance to the chloramphenicol antibiotic." ;
  rdfs:subClassOf obo:ECO_0000049 .

obo:ECO_0006249 a rdfs:Class ;
  rdfs:label "bait-prey protein pull-down evidence" ;
  rdfs:comment "A type of bait-prey interaction evidence resulting from an affinity purification in-vitro technique used to detect physical interactions between two or more proteins, where a molecule of interest - a \"bait\" - is immobilized and incubated with a protein source containing putative \"prey\" proteins." ;
  rdfs:subClassOf obo:ECO_0006351 .

obo:ECO_0000128 a rdfs:Class ;
  rdfs:label "yellow fluorescent protein fusion protein localization evidence" ;
  rdfs:comment "A type of fusion protein localization evidence resulting from the labeling of a protein of interest with yellow fluorescent protein (YFP), which was created by mutating green fluorescent protein (GFP) of the Aequorea victoria." ;
  rdfs:subClassOf obo:ECO_0000124 .

obo:ECO_0007616 a rdfs:Class ;
  rdfs:label "confocal laser scanning microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of confocal laser scanning microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007059 , obo:ECO_0007490 .

obo:ECO_0000207 a rdfs:Class ;
  rdfs:label "nucleotide BLAST evidence" ;
  rdfs:comment "A type of BLAST evidence in which nucleotide sequences are aligned." ;
  rdfs:subClassOf obo:ECO_0000206 .

obo:ECO_0007406 a rdfs:Class ;
  rdfs:label "in vitro sumoylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro sumoylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001070 , obo:ECO_0007788 .

obo:ECO_0001094 a rdfs:Class ;
  rdfs:label "macroscopy evidence" ;
  rdfs:comment "A type of experimental evidence that is visible to the naked eye cf. microscopy evidence which requires the aid of a microscope." ;
  rdfs:subClassOf obo:ECO_0000006 .

obo:ECO_0007472 a rdfs:Class ;
  rdfs:label "high-performance liquid chromatography evidence used in automatic assertion" ;
  rdfs:comment "A type of high-performance liquid chromatography evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001052 , obo:ECO_0007681 .

obo:ECO_0006330 a rdfs:Class ;
  rdfs:label "static fluorescence quenching evidence used in automatic assertion" ;
  rdfs:comment "A type of static fluorescence quenching evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006274 , obo:ECO_0007332 , obo:ECO_0007450 .

obo:GO_0010628 a rdfs:Class ;
  rdfs:label "positive regulation of gene expression" ;
  rdfs:subClassOf obo:GO_0010468 , obo:GO_0010604 , obo:GO_0065007 .

obo:ECO_0000098 a rdfs:Class ;
  rdfs:label "obsolete in situ hybridization evidence" ;
  rdfs:comment "OBSOLETE. A type of nucleic acid hybridization evidence based on localization of a specific segment of DNA or RNA by the application of a complementary strand of nucleic acid to which a reporter molecule is attached." .

obo:ECO_0006096 a rdfs:Class ;
  rdfs:label "epistatic interaction phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of epistatic interaction phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000056 , obo:ECO_0000316 .

obo:OBI_0001460 a rdfs:Class ;
  rdfs:label "genetic modification design" ;
  rdfs:comment "A study design in which an organism(s) is studied that has had genetic material removed, rearranged, mutagenized or added, such as in a knock out." ;
  rdfs:subClassOf obo:OBI_0000115 .

obo:OBI_0001136 a rdfs:Class ;
  rdfs:label "surface plasmon resonance instrument" ;
  rdfs:comment "A tool for measuring adsorption of material onto planar metal (typically gold and silver) surfaces or onto the surface of metal nanoparticles." ;
  rdfs:subClassOf obo:OBI_0000832 .

obo:GO_0051239 a rdfs:Class ;
  rdfs:label "regulation of multicellular organismal process" ;
  rdfs:subClassOf obo:GO_0050789 , obo:GO_0065007 .

obo:ECO_0007358 a rdfs:Class ;
  rdfs:label "carboxyfluorescein diacetate succinimidyl ester staining evidence used in automatic assertion" ;
  rdfs:comment "A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001022 , obo:ECO_0007798 .

obo:ECO_0000168 a rdfs:Class ;
  rdfs:label "transcription assay evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from the detection and quantification of transcribed RNA." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:OBI_0000367 a rdfs:Class ;
  rdfs:label "light emission function"@en ;
  rdfs:comment "A light emission function is an excitation function to excite a material to a specific excitation state that it emits light."@en ;
  rdfs:subClassOf obo:OBI_0000374 .

obo:ECO_0008004 a rdfs:Class ;
  rdfs:label "machine learning method evidence used in automatic assertion" ;
  rdfs:comment "A type of machine learning method evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007669 , obo:ECO_0008000 .

obo:GO_0050922 a rdfs:Class ;
  rdfs:label "negative regulation of chemotaxis" ;
  rdfs:subClassOf obo:GO_0032102 , obo:GO_0040013 , obo:GO_0050920 , obo:GO_0065007 .

obo:ECO_0008123 a rdfs:Class ;
  rdfs:label "antibody DT9-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody DT9-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008057 , obo:ECO_0008087 .

obo:ECO_0007443 a rdfs:Class ;
  rdfs:label "phage display evidence used in automatic assertion" ;
  rdfs:comment "A type of phage display evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001108 , obo:ECO_0007373 .

obo:OBI_0001145 a rdfs:Class ;
  rdfs:label "3D structure determination of bound molecular complex assay" ;
  rdfs:comment "A 3D molecular structure determination assay in which a complex of 2 or more molecular enties is characterized which provides information on their binding configuration." ;
  rdfs:subClassOf obo:OBI_0003117 .

obo:ECO_0007076 a rdfs:Class ;
  rdfs:label "phenomic profiling assay evidence used in manual assertion" ;
  rdfs:comment "A type of phenomic profiling assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007075 , obo:ECO_0007634 .

obo:ECO_0006209 a rdfs:Class ;
  rdfs:label "cryogenic electron microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of cryogenic electron microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006181 , obo:ECO_0007493 .

obo:ECO_0000058 a rdfs:Class ;
  rdfs:label "expression microarray evidence" ;
  rdfs:comment "A type of transcript expression evidence resulting from simultaneous profiling of the expression levels of thousands of genes in a single experiment allowing analysis of genes and their networks." ;
  rdfs:subClassOf obo:ECO_0000009 , obo:IAO_0000030 .

obo:OBI_0000685 a rdfs:Class ;
  rdfs:label "creating a mixture of molecules in solution" ;
  rdfs:comment "is a process with the objective to prepare a liquid solution of one or more chemicals at desired concentrations." ;
  rdfs:subClassOf obo:OBI_0000652 .

obo:ECO_0007483 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence based on paralogy evidence used in automatic assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence based on paralogy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005550 , obo:ECO_0007482 .

obo:ECO_0007036 a rdfs:Class ;
  rdfs:label "copper transport assay evidence used in manual assertion" ;
  rdfs:comment "A type of copper transport assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0007035 .

obo:ECO_0007772 a rdfs:Class ;
  rdfs:label "phosphatase assay evidence used in automatic assertion" ;
  rdfs:comment "A type of phosphatase assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001556 , obo:ECO_0007307 .

obo:ECO_0006051 a rdfs:Class ;
  rdfs:label "morpholino experiment evidence used in manual assertion" ;
  rdfs:comment "A type of morpholino experiment evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000292 , obo:ECO_0000315 .

obo:ECO_0007432 a rdfs:Class ;
  rdfs:label "bait-prey hybrid interaction evidence used in automatic assertion" ;
  rdfs:comment "A type of bait-prey hybrid interaction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000025 , obo:ECO_0006393 .

obo:GO_1903625 a rdfs:Class ;
  rdfs:label "negative regulation of DNA catabolic process" ;
  rdfs:subClassOf obo:GO_0031330 , obo:GO_0051053 , obo:GO_0065007 , obo:GO_1903624 .

obo:GO_1902339 a rdfs:Class ;
  rdfs:label "positive regulation of apoptotic process involved in morphogenesis" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_1902337 , obo:GO_1904747 .

obo:ECO_0005615 a rdfs:Class ;
  rdfs:label "alkaline phosphatase reporter gene assay evidence used in manual assertion" ;
  rdfs:comment "A type of alkaline phosphatase reporter gene assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000270 , obo:ECO_0001801 .

obo:GO_0009896 a rdfs:Class ;
  rdfs:label "positive regulation of catabolic process" ;
  rdfs:subClassOf obo:GO_0009893 , obo:GO_0009894 , obo:GO_0065007 .

obo:OBI_0002186 a rdfs:Class ;
  rdfs:label "electroencephalography" ;
  rdfs:comment "An extracellular electrophysiology assay where electrodes are mounted outside the brain (either on the surface of the scalp on onto the brain surface itself during surgery) to measure the electrical field over the external surface." ;
  rdfs:subClassOf obo:OBI_0000454 .

obo:OBI_0200166 a rdfs:Class ;
  rdfs:label "data transformation objective"@en ;
  rdfs:comment "An objective specification to transformation input data into output data"@en ;
  rdfs:subClassOf obo:IAO_0000005 .

obo:ECO_0000248 a rdfs:Class ;
  rdfs:label "sequence alignment evidence used in automatic assertion" ;
  rdfs:comment "A type of sequence alignment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000200 , obo:ECO_0000249 .

obo:ECO_0007823 a rdfs:Class ;
  rdfs:label "3C evidence used in automatic assertion" ;
  rdfs:comment "A type of 3C evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000233 , obo:ECO_0007822 .

obo:ECO_0008032 a rdfs:Class ;
  rdfs:label "curator inference based on intrinsic disorder prediction" ;
  rdfs:comment "A type of curator inference in which information from intrinsic disorder prediction tools has been employed." ;
  rdfs:subClassOf obo:ECO_0000205 , obo:ECO_0000212 .

obo:OBI_0001398 a rdfs:Class ;
  rdfs:label "protein and DNA interaction identification objective"@en ;
  rdfs:comment "A sequence feature identification objective that aims to characterize the interactions between protein and DNA which includes identification of transcription factor binding sites."@en ;
  rdfs:subClassOf obo:OBI_0000113 .

obo:GO_0032074 a rdfs:Class ;
  rdfs:label "negative regulation of nuclease activity" ;
  rdfs:subClassOf obo:GO_0032069 , obo:GO_0045934 , obo:GO_0051346 , obo:GO_0065007 .

obo:ECO_0007562 a rdfs:Class ;
  rdfs:label "over expression analysis evidence used in automatic assertion" ;
  rdfs:comment "A type of over expression analysis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000120 , obo:ECO_0007427 .

obo:ECO_0005648 a rdfs:Class ;
  rdfs:label "luciferase reporter gene assay evidence used in manual assertion" ;
  rdfs:comment "A type of luciferase reporter gene assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000270 , obo:ECO_0001805 .

obo:ECO_0007412 a rdfs:Class ;
  rdfs:label "in vivo deacetylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo deacetylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001076 , obo:ECO_0007771 .

obo:GO_1904746 a rdfs:Class ;
  rdfs:label "negative regulation of apoptotic process involved in development" ;
  rdfs:subClassOf obo:GO_0043066 , obo:GO_0051093 , obo:GO_0065007 , obo:GO_1904748 .

obo:ECO_0008094 a rdfs:Class ;
  rdfs:label "antibody 28-14-8S-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody 28-14-8S-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008042 , obo:ECO_0008088 .

obo:ECO_0007718 a rdfs:Class ;
  rdfs:label "ubiquitination assay evidence used in manual assertion" ;
  rdfs:comment "A type of ubiquitination assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001562 .

obo:OBI_0600050 a rdfs:Class ;
  rdfs:label "specific enzymatic cleavage"@en ;
  rdfs:comment "an enzymatic cleavage that uses an enzyme with a strong affinity for specific cleavage sites in the input material"@en ;
  rdfs:subClassOf obo:OBI_0000231 .

obo:ECO_0000355 a rdfs:Class ;
  rdfs:label "phylogenetic distribution evidence" ;
  rdfs:comment "A type of phylogenetic evidence characterized by the mapping of the character states of living organisms onto phylogenies using the method of maximum parsimony." ;
  rdfs:subClassOf obo:ECO_0000080 .

obo:ECO_0000262 a rdfs:Class ;
  rdfs:label "targeting sequence prediction evidence used in manual assertion" ;
  rdfs:comment "A type of targeting sequence prediction that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000081 , obo:ECO_0000257 .

obo:ECO_0007541 a rdfs:Class ;
  rdfs:label "biofilm formation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of biofilm formation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006020 , obo:ECO_0007775 .

obo:OBI_0000807 a rdfs:Class ;
  rdfs:label "presentation of stimulus" ;
  rdfs:comment "a planned process in which an organism is exposed to some stimulus with the intent to invoke an action" ;
  rdfs:subClassOf obo:OBI_0000011 .

obo:GO_0032103 a rdfs:Class ;
  rdfs:label "positive regulation of response to external stimulus" ;
  rdfs:subClassOf obo:GO_0032101 , obo:GO_0048584 , obo:GO_0065007 .

obo:ECO_0007660 a rdfs:Class ;
  rdfs:label "automatically integrated combinatorial experimental evidence used in automatic assertion" ;
  rdfs:comment "A type of automatically integrated combinatorial experimental evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000053 , obo:ECO_0007658 , obo:ECO_0007830 .

obo:GO_0097194 a rdfs:Class ;
  rdfs:label "execution phase of apoptosis" ;
  rdfs:subClassOf obo:GO_0009987 .

obo:ECO_0007203 a rdfs:Class ;
  rdfs:label "gene expression similarity evidence used in automatic assertion" ;
  rdfs:comment "A type of gene expression similarity evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000075 , obo:ECO_0007824 .

obo:ECO_0006040 a rdfs:Class ;
  rdfs:label "genetically encoded fluorescent ion concentration sensor assay evidence" ;
  rdfs:comment "A type of genetically encoded fluorescent electrophysiology assay evidence that is based on direct, quantitative measurement of ion concentration using a genetically encoded fluorescent sensor." ;
  rdfs:subClassOf obo:ECO_0006038 .

obo:ECO_0001099 a rdfs:Class ;
  rdfs:label "motility wound healing assay evidence" ;
  rdfs:comment "A type of cell-based assay evidence to measure cell motility in which a \"wound\" is created in a cell monolayer and the migration of the cells is captured by imaging at regular intervals during cell migration to close the wound." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:GO_0051053 a rdfs:Class ;
  rdfs:label "negative regulation of DNA metabolic process" ;
  rdfs:subClassOf obo:GO_0010605 , obo:GO_0045934 , obo:GO_0051052 , obo:GO_0065007 .

obo:ECO_0007595 a rdfs:Class ;
  rdfs:label "photoconversion evidence used in automatic assertion" ;
  rdfs:comment "A type of photoconversion evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007017 , obo:ECO_0007450 .

obo:GO_0031328 a rdfs:Class ;
  rdfs:label "positive regulation of cellular biosynthetic process" ;
  rdfs:subClassOf obo:GO_0009891 , obo:GO_0031325 , obo:GO_0031326 , obo:GO_0065007 .

obo:GO_0043067 a rdfs:Class ;
  rdfs:label "regulation of programmed cell death" ;
  rdfs:subClassOf obo:GO_0010941 , obo:GO_0065007 .

obo:ECO_0008131 a rdfs:Class ;
  rdfs:label "antibody ILA-21-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody ILA-21-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008061 , obo:ECO_0008087 .

obo:GO_0046942 a rdfs:Class ;
  rdfs:label "carboxylic acid transport" ;
  rdfs:subClassOf obo:GO_0015711 .

obo:OBI_0000921 a rdfs:Class ;
  rdfs:label "labeled RNA extract" ;
  rdfs:comment "a labeled specimen that is the output of a labeling process and has grain labeled RNA to be able to detect RNA in future experiments." ;
  rdfs:subClassOf obo:OBI_0001143 .

obo:ECO_0000348 a rdfs:Class ;
  rdfs:label "full support of intron positions by RNA-sequencing alignment evidence used in automatic assertion" ;
  rdfs:comment "A type of full support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000343 , obo:ECO_0000347 .

obo:ECO_0008116 a rdfs:Class ;
  rdfs:label "antibody BB7.2-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody BB7.2-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008053 , obo:ECO_0008088 .

obo:ECO_0001534 a rdfs:Class ;
  rdfs:label "nucleic acid localization evidence" ;
  rdfs:comment "A type of localization evidence based on localization of a specific segment of DNA or RNA within tissue by the application of a complementary strand of nucleic acid to which a reporter molecule (i.e. either radio-, fluorescent- or antigen-labeled probe) is attached and quantified using either autoradiography, fluorescence microscopy, or immunohistochemistry." ;
  rdfs:subClassOf obo:ECO_0001533 .

obo:ECO_0006168 a rdfs:Class ;
  rdfs:label "nuclear magnetic resonance imaging evidence used in manual assertion" ;
  rdfs:comment "A type of nuclear magnetic resonance imaging evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001238 , obo:ECO_0006166 .

obo:ECO_0007342 a rdfs:Class ;
  rdfs:label "alamarBlue assay evidence used in automatic assertion" ;
  rdfs:comment "A type of alamarBlue assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001008 , obo:ECO_0007774 .

obo:ECO_0006287 a rdfs:Class ;
  rdfs:label "electron paramagnetic resonance evidence used in manual assertion" ;
  rdfs:comment "A type of electron paramagnetic resonance evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006253 , obo:ECO_0006285 .

obo:ECO_0001179 a rdfs:Class ;
  rdfs:label "dominant-negative mutant phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of dominant-negative mutant phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0001042 .

obo:ECO_0005503 a rdfs:Class ;
  rdfs:label "two-dimensional polyacrylamide gel electrophoresis evidence" ;
  rdfs:comment "A type of gel electrophoresis evidence that involves one dimension electrophoresis where biomolecules are electrophoresed on a low percentage polyacrylamide gel  to be separated in proportion to their mass or isoelectric point, followed by high voltage electrophoresis on a higher percentage gel in the presence of ethidium bromide to alter non-linear molecular shape." ;
  rdfs:subClassOf obo:ECO_0000337 .

obo:CHEBI_23367 a rdfs:Class ;
  rdfs:label "molecular entity" ;
  rdfs:comment "Any constitutionally or isotopically distinct atom, molecule, ion, ion pair, radical, radical ion, complex, conformer etc., identifiable as a separately distinguishable entity." ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:ECO_0006154 a rdfs:Class ;
  rdfs:label "self-reported patient statement evidence" ;
  rdfs:comment "A type of self-reported individual's statement evidence in which information is provided by a patient in a clinical setting." ;
  rdfs:subClassOf obo:ECO_0006153 .

obo:GO_0009895 a rdfs:Class ;
  rdfs:label "negative regulation of catabolic process" ;
  rdfs:subClassOf obo:GO_0009892 , obo:GO_0009894 , obo:GO_0065007 .

obo:ECO_0000017 a rdfs:Class ;
  rdfs:label "ectopic expression evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence where a transgenic strain carrying the construct of a promoter cDNA fusion in which a gene of interest is driven by a defined promoter or enhancer is ectopically expressed in the defined pattern to characterize potential cellular properties and functions of a protein of interest." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:GO_0051098 a rdfs:Class ;
  rdfs:label "regulation of binding" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_0065009 .

obo:GO_0034766 a rdfs:Class ;
  rdfs:label "negative regulation of ion transmembrane transport" ;
  rdfs:subClassOf obo:GO_0034763 , obo:GO_0034765 , obo:GO_0043271 , obo:GO_0065007 .

obo:GO_0006308 a rdfs:Class ;
  rdfs:label "DNA catabolic process" ;
  rdfs:subClassOf obo:GO_0006259 , obo:GO_0034655 , obo:GO_0044265 .

obo:ECO_0000024 a rdfs:Class ;
  rdfs:label "protein binding evidence" ;
  rdfs:comment "A type of affinity evidence resulting from the binding of a molecule to a protein or protein complex." ;
  rdfs:subClassOf obo:ECO_0000023 .

obo:OBI_0001211 a rdfs:Class ;
  rdfs:label "ex vivo design" ;
  rdfs:comment "A study design where all or part of an organism is removed and studied in vitro, e.g. part of a mouse is removed and cultured in vitro. A cell culture with an established cell line is an in vitro experiment." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:OBI_0000079 a rdfs:Class ;
  rdfs:label "culture medium"@en ;
  rdfs:comment "a processed material that provides the needed nourishment for microorganisms or cells grown in vitro." ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:ECO_0005546 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence based on paralogy evidence used in manual assertion" ;
  rdfs:comment "A type of  biological system reconstruction evidence based on paralogy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005550 , obo:ECO_0005610 .

obo:PATO_0001741 a rdfs:Class ;
  rdfs:label "radioactive" ;
  rdfs:comment "A radiation quality inhering in bearer by virtue of the bearer's exhibiting or being caused by radioactivity." ;
  rdfs:subClassOf obo:PATO_0001740 .

obo:OBI_0001633 a rdfs:Class ;
  rdfs:label "purified material" ;
  rdfs:comment "A material entity that is generated by a purification process in which an input material is separated to obtain a fraction with a higher concentration of a desired component" ;
  rdfs:subClassOf obo:BFO_0000040 , obo:OBI_0000047 .

obo:ECO_0007232 a rdfs:Class ;
  rdfs:label "chain termination sequencing evidence used in automatic assertion" ;
  rdfs:comment "A type of chain termination sequencing evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000225 , obo:ECO_0007813 .

obo:OBI_1110122 a rdfs:Class ;
  rdfs:label "pathologic process"@en ;
  rdfs:comment "abnormal, harmful processes caused by or associated with a disease"@en ;
  rdfs:subClassOf obo:GO_0008150 .

obo:ECO_0007841 a rdfs:Class ;
  rdfs:label "curator inference from Merriam-Webster Dictionary used in automatic assertion" ;
  rdfs:comment "A type of curator inference from Merriam-Webster Dictionary that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007643 , obo:ECO_0007839 .

obo:ECO_0008025 a rdfs:Class ;
  rdfs:label "neural network method evidence used in automatic assertion" ;
  rdfs:comment "A type of neural network method evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008004 , obo:ECO_0008013 .

obo:ECO_0007004 a rdfs:Class ;
  rdfs:label "high throughput direct assay evidence" ;
  rdfs:comment "A type of high throughput evidence derived from the high throughput direct measurement of some aspect of a biological feature." ;
  rdfs:subClassOf obo:ECO_0000002 , obo:ECO_0006055 .

obo:ECO_0005804 a rdfs:Class ;
  rdfs:label "immunofluorescence evidence used in manual assertion" ;
  rdfs:comment "A type of immunofluorescence evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000007 , obo:ECO_0000269 .

obo:ECO_0001002 a rdfs:Class ;
  rdfs:label "[3H]arachidonic acid release assay evidence" ;
  rdfs:comment "A type of cell-based assay evidence resulting from labeling cells with [3H]arachidonic acid and subsequently determining the release of the arachidonic acid by the cells." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:GO_0051050 a rdfs:Class ;
  rdfs:label "positive regulation of transport" ;
  rdfs:subClassOf obo:GO_0048518 , obo:GO_0051049 , obo:GO_0065007 .

obo:ECO_0008195 a rdfs:Class ;
  rdfs:label "avidity-based extracellular interaction screening evidence used in automatic assertion" ;
  rdfs:comment "A type of avidity-based extracellular interaction screening evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008186 , obo:ECO_0008193 .

obo:ECO_0000087 a rdfs:Class ;
  rdfs:label "immunolocalization evidence" ;
  rdfs:comment "A type of microscopy evidence where antibodies are used to detect the location of molecules or other structures within cells or tissues." ;
  rdfs:subClassOf obo:ECO_0001098 , obo:ECO_0005593 .

obo:ECO_0007423 a rdfs:Class ;
  rdfs:label "in vivo transcription assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo transcription assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001087 , obo:ECO_0007768 .

obo:ECO_0005540 a rdfs:Class ;
  rdfs:label "obsolete computationally derived logical inference from automatic assertion used in automatic assertion" .

obo:ECO_0006339 a rdfs:Class ;
  rdfs:label "structural ensemble generation evidence" ;
  rdfs:comment "A type of computational structure modeling evidence used to build molecular models of intrinsically disordered proteins to capture the dynamic behaviour of their polypeptide chains. Such tools include EOM, ASTEROIDS and ENSEMBLE." ;
  rdfs:subClassOf obo:ECO_0006341 .

obo:ECO_0007365 a rdfs:Class ;
  rdfs:label "colony counting evidence used in automatic assertion" ;
  rdfs:comment "A type of colony counting evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001027 , obo:ECO_0007307 .

obo:OBI_0000416 a rdfs:Class ;
  rdfs:label "cloning insert role"@en ;
  rdfs:comment "cloning insert role is a role which inheres in DNA or RNA and is realized by the process of being inserted into a cloning vector in a cloning process."@en ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:ECO_0001837 a rdfs:Class ;
  rdfs:label "in situ hybridization evidence used in automatic assertion" ;
  rdfs:comment "A type of in situ hybridization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001836 , obo:ECO_0007298 .

obo:OBI_0000722 a rdfs:Class ;
  rdfs:label "paired-end library" ;
  rdfs:comment "is a collection of short paired tags from the two ends of DNA fragments are extracted and covalently linked as ditag constructs" ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:ECO_0000008 a rdfs:Class ;
  rdfs:label "expression pattern evidence" ;
  rdfs:comment "A type of experimental evidence that is based on characterization of gene expression." ;
  rdfs:subClassOf obo:ECO_0000006 .

obo:ECO_0007696 a rdfs:Class ;
  rdfs:label "cell proliferation assay evidence used in manual assertion" ;
  rdfs:comment "A type of cell proliferation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005007 , obo:ECO_0007695 .

obo:OBI_0000378 a rdfs:Class ;
  rdfs:label "filter function"@en ;
  rdfs:comment "A filter function is a function to prevent the flow of certain entities based on a quality or qualities of the entity while allowing entities which have different qualities to pass through"@en ;
  rdfs:subClassOf obo:OBI_0000372 .

obo:ECO_0007294 a rdfs:Class ;
  rdfs:label "obsolete inference from phenotype manipulation evidence used in automatic assertion" ;
  rdfs:comment "OBSOLETE. A type of inference from phenotype manipulation evidence that is used in an automatic assertion." .

obo:GO_0042277 a rdfs:Class ;
  rdfs:label "peptide binding" ;
  rdfs:subClassOf obo:GO_0033218 .

obo:GO_0050790 a rdfs:Class ;
  rdfs:label "regulation of catalytic activity" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_0065009 .

obo:ECO_0001601 a rdfs:Class ;
  rdfs:label "protein-oligonucleotide microarray binding evidence" ;
  rdfs:comment "A type of protein-binding evidence that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites." ;
  rdfs:subClassOf obo:ECO_0000024 , obo:ECO_0000136 .

obo:GO_0050921 a rdfs:Class ;
  rdfs:label "positive regulation of chemotaxis" ;
  rdfs:subClassOf obo:GO_0032103 , obo:GO_0040017 , obo:GO_0050920 , obo:GO_0065007 .

obo:ECO_0006251 a rdfs:Class ;
  rdfs:label "electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence" ;
  rdfs:comment "A type of mass spectrometry evidence resulting from the ionization of polar functional groups producing multiple charged ions followed by the detection of ion cyclotron frequencies within a magnetic field, making it suitable for the study of large molecules (>2 kDa)." ;
  rdfs:subClassOf obo:ECO_0001096 .

obo:GO_0048518 a rdfs:Class ;
  rdfs:label "positive regulation of biological process" ;
  rdfs:subClassOf obo:GO_0050789 , obo:GO_0065007 .

obo:ECO_0008083 a rdfs:Class ;
  rdfs:label "T cell epitope assay type based MHC restriction evidence" ;
  rdfs:comment "A type of direct assay evidence that employs the type of T cell epitope assay to determine that a specific ligand is restricted by a MHC molecule or class of MHC molecules." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:GO_0051172 a rdfs:Class ;
  rdfs:label "negative regulation of nitrogen compound metabolic process" ;
  rdfs:subClassOf obo:GO_0009892 , obo:GO_0051171 , obo:GO_0065007 .

obo:ECO_0005521 a rdfs:Class ;
  rdfs:label "S1 nuclease protection assay evidence" ;
  rdfs:comment "A type of nuclease protection assay evidence where RNA is hybridized with complementary DNA probes and exposed to S1 nuclease for unbound RNA degradation after which the intact RNA is run on a gel for probe size determination and RNA identification to detect and map specific RNAs in a complex mixture of total cellular RNA." ;
  rdfs:subClassOf obo:ECO_0001106 .

obo:ECO_0005012 a rdfs:Class ;
  rdfs:label "cytotoxicity assay evidence" ;
  rdfs:comment "A type of  cell-based assay evidence resulting from the ability of an agent to induce cellular necrosis or apoptosis and the subsequent analysis of cell death mechanism." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:ECO_0007739 a rdfs:Class ;
  rdfs:label "RNA detection assay evidence used in manual assertion" ;
  rdfs:comment "A type of RNA detection assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0005518 .

obo:GO_0060561 a rdfs:Class ;
  rdfs:label "apoptotic process involved in morphogenesis" ;
  rdfs:subClassOf obo:GO_0006915 , obo:GO_1902742 .

obo:ECO_0005637 a rdfs:Class ;
  rdfs:label "green fluorescent protein reporter gene assay evidence used in automatic assertion" ;
  rdfs:comment "A type of green fluorescent protein reporter gene assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001816 , obo:ECO_0007765 .

obo:ECO_0007530 a rdfs:Class ;
  rdfs:label "cell transfection experiment evidence used in automatic assertion" ;
  rdfs:comment "A type of cell transfection experiment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005606 , obo:ECO_0007297 .

obo:GO_0051248 a rdfs:Class ;
  rdfs:label "negative regulation of protein metabolic process" ;
  rdfs:subClassOf obo:GO_0010605 , obo:GO_0051172 , obo:GO_0051246 , obo:GO_0065007 .

obo:OBI_0001986 a rdfs:Class ;
  rdfs:label "immunohistochemistry" ;
  rdfs:comment "An immunostaining assay to detect and potentially localize antigens within the cells of a tissue section." ;
  rdfs:subClassOf obo:OBI_0001632 .

obo:ECO_0006211 a rdfs:Class ;
  rdfs:label "small-angle X-ray scattering evidence used in automatic assertion" ;
  rdfs:comment "A type of small-angle X-ray scattering evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006182 , obo:ECO_0007617 .

obo:ECO_0007648 a rdfs:Class ;
  rdfs:label "manually integrated combinatorial computational evidence" ;
  rdfs:comment "A type of manually integrated combinatorial evidence in which two or more distinct types of computational evidence are integrated manually." ;
  rdfs:subClassOf obo:ECO_0007674 , obo:ECO_0007677 .

obo:ECO_0007830 a rdfs:Class ;
  rdfs:label "combinatorial experimental evidence used in automatic assertion" ;
  rdfs:comment "A type of combinatorial experimental evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000213 , obo:ECO_0007654 .

obo:ECO_0000045 a rdfs:Class ;
  rdfs:label "spatial pattern of protein expression evidence" ;
  rdfs:comment "A type of protein expression evidence that accounts for the position of RNA translation to a protein product ranging in scale from differing tissues to regions of an anatomical structure." ;
  rdfs:subClassOf obo:ECO_0000010 .

obo:OBI_0000789 a rdfs:Class ;
  rdfs:label "survival rate" ;
  rdfs:comment "A measurement data that represents the percentage of people or animals in a study or treatment group who are alive for a given period of time after diagnosis or initiation of monitoring." ;
  rdfs:subClassOf obo:IAO_0000109 .

obo:ECO_0001088 a rdfs:Class ;
  rdfs:label "in vivo translation assay evidence" ;
  rdfs:comment "A type of translation assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001561 .

obo:ECO_0000063 a rdfs:Class ;
  rdfs:label "compositional similarity evidence" ;
  rdfs:comment "A type of phenotypic similarity evidence based on the similarity of the histological makeup of structures." ;
  rdfs:subClassOf obo:ECO_0000057 .

obo:ECO_0005004 a rdfs:Class ;
  rdfs:label "cell viability assay evidence" ;
  rdfs:comment "A type of cell-based assay evidence resulting from analyzing the ability of cells to survive or live successfully." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:ECO_0006007 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation-chip evidence used in manual assertion" ;
  rdfs:comment "A type of chromatin immunoprecipitation-chip evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000230 , obo:ECO_0005644 .

obo:ECO_0007783 a rdfs:Class ;
  rdfs:label "methylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of methylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001554 , obo:ECO_0007307 .

obo:GO_0010639 a rdfs:Class ;
  rdfs:label "negative regulation of organelle organization" ;
  rdfs:subClassOf obo:GO_0033043 , obo:GO_0051129 , obo:GO_0065007 .

obo:ECO_0000085 a rdfs:Class ;
  rdfs:label "immunoprecipitation evidence" ;
  rdfs:comment "A type of protein binding evidence where an antibody specific for a target protein is used to precipitate the target protein along with any other molecules (e.g. protein, nucleic acid) that are bound to the target protein." ;
  rdfs:subClassOf obo:ECO_0000024 .

obo:ECO_0000222 a rdfs:Class ;
  rdfs:label "Illumina sequencing evidence" ;
  rdfs:comment "A type of high throughput nucleotide sequencing evidence in which sequence is determined through hybridization of adaptor-ligated DNA to a glass slide on which each fragment is simultaneously clonally amplified, followed by progressive rounds of base incorporation with fluorescently-tagged nucleotides, washing, imaging, and cleavage." ;
  rdfs:subClassOf obo:ECO_0000221 .

obo:ECO_0006240 a rdfs:Class ;
  rdfs:label "colony boundary assay evidence" ;
  rdfs:comment "A type of colony morphology phenotypic evidence based on the presence or absence of a visually apparent boundary formed between colonies of multiple isolates or strains of the same microbial species growing on a solid surface such as that of an agar culture medium." ;
  rdfs:subClassOf obo:ECO_0007077 .

obo:ECO_0000038 a rdfs:Class ;
  rdfs:label "transient rescue experiment evidence" ;
  rdfs:comment "A type of functional complementation evidence resulting from the introduction of nucleic acids, which are not permanently incorporated into the genome, to temporarily prevent, or \"rescue\" an organism from a condition." ;
  rdfs:subClassOf obo:ECO_0000012 .

obo:OBI_0001007 a rdfs:Class ;
  rdfs:label "image creation" ;
  rdfs:comment "A planned process that captures an image of an object." ;
  rdfs:subClassOf obo:OBI_0000011 .

obo:ECO_0007214 a rdfs:Class ;
  rdfs:label "thin layer chromatography evidence used in automatic assertion" ;
  rdfs:comment "A type of thin layer chromatography evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000140 , obo:ECO_0007681 .

obo:OBI_0001691 a rdfs:Class ;
  rdfs:label "cellular structure feature identification objective"@en ;
  rdfs:comment "is an objective specification which aims to discover cellular structure properties"@en ;
  rdfs:subClassOf obo:OBI_0000015 .

obo:ECO_0006270 a rdfs:Class ;
  rdfs:label "RNA-protein binding evidence" ;
  rdfs:comment "A type of affinity evidence resulting from the qualitative and quantitative analysis of the affinity with which a protein binds to RNA." ;
  rdfs:subClassOf obo:ECO_0000024 , obo:ECO_0000136 .

obo:OBI_0400110 a rdfs:Class ;
  rdfs:label "hybridization chamber" ;
  rdfs:comment "A device which is used to maintain constant contact of a liquid on an array.  This can be either a glass vial or slide." ;
  rdfs:subClassOf obo:OBI_0002089 .

obo:ECO_0007685 a rdfs:Class ;
  rdfs:label "substance quantification evidence used in manual assertion" ;
  rdfs:comment "A type of substance quantification evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0000335 .

obo:ECO_0007333 a rdfs:Class ;
  rdfs:label "gene neighbors evidence used in automatic assertion" ;
  rdfs:comment "A type of gene neighbors evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000084 , obo:ECO_0007327 .

obo:ECO_0005569 a rdfs:Class ;
  rdfs:label "obsolete window current trace evidence" .

obo:OBI_0001503 a rdfs:Class ;
  rdfs:label "MHC protein complex binding to ligand" ;
  rdfs:comment "The process in which a ligand binds to an MHC molecule to form a stable complex " .

obo:ECO_0006200 a rdfs:Class ;
  rdfs:label "circular dichroism evidence used in manual assertion" ;
  rdfs:comment "A type of circular dichroism evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0006177 .

obo:GO_0017148 a rdfs:Class ;
  rdfs:label "negative regulation of translation" ;
  rdfs:subClassOf obo:GO_0006417 , obo:GO_0010629 , obo:GO_0032269 , obo:GO_0034249 , obo:GO_0065007 , obo:GO_2000113 .

obo:ECO_0008134 a rdfs:Class ;
  rdfs:label "antibody ILA-88-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody ILA-88-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008062 , obo:ECO_0008088 .

obo:ECO_0008015 a rdfs:Class ;
  rdfs:label "inference of intermediate molecular structure from structure of final molecule" ;
  rdfs:comment "A type of curator inference in which the structure of a possible intermediate molecule made during a biosynthetic process is inferred based on the structure of the final molecule in the process." ;
  rdfs:subClassOf obo:ECO_0000205 .

obo:ECO_0006039 a rdfs:Class ;
  rdfs:label "genetically encoded fluorescent electrophysiology assay evidence used in manual assertion" ;
  rdfs:comment "A type of genetically encoded fluorescent electrophysiology assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006006 , obo:ECO_0006035 , obo:ECO_0006038 .

obo:ECO_0000326 a rdfs:Class ;
  rdfs:label "transcript splice pattern evidence" ;
  rdfs:comment "A type of sequence alignment evidence that is based on comparing the position of exon junctions, relative to a reference genome, for a transcript annotation and a supporting transcript." ;
  rdfs:subClassOf obo:ECO_0000200 .

obo:ECO_0001023 a rdfs:Class ;
  rdfs:label "chemiluminescence-linked immunoassay evidence" ;
  rdfs:comment "A type of protein detection assay evidence resulting from a complex (a chemiluminescent compound, protein and a steroidal hapten) utilized as a labeled antigen, which emits light when treated with hydrogen peroxide and copper acetate at a high pH." ;
  rdfs:subClassOf obo:ECO_0005517 .

obo:GO_1902510 a rdfs:Class ;
  rdfs:label "regulation of apoptotic DNA fragmentation" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_1903624 .

obo:ECO_0000069 a rdfs:Class ;
  rdfs:label "differential methylation hybridization evidence" ;
  rdfs:comment "A type of in vitro methylation assay evidence where methylation-sensitive restriction enzymes are utilized to compare differential methylation between two samples by first shearing DNA, followed by end-blunting, ligation of linkers, methylation sensitive restriction, PCR using linker primers, dye labeling and relative quantification of methylated DNA fragments by two-colored array hybridization to a CpG island microarray for visual assessment." ;
  rdfs:subClassOf obo:ECO_0001065 .

obo:IAO_0000104 a rdfs:Class ;
  rdfs:label "plan specification"@en ;
  rdfs:comment "A directive information entity with action specifications and objective specifications as parts that, when concretized, is realized in a process in which the bearer tries to achieve the objectives by taking the actions specified."@en ;
  rdfs:subClassOf obo:IAO_0000033 .

obo:ECO_0006057 a rdfs:Class ;
  rdfs:label "high throughput evidence used in automatic assertion" ;
  rdfs:comment "A type of evidence that is used in an automatic assertion where data generation is automated with equipment to allow for assaying samples or molecules in parallel." ;
  rdfs:subClassOf obo:ECO_0000501 , obo:ECO_0006055 .

obo:ECO_0000211 a rdfs:Class ;
  rdfs:label "protein BLAST evidence used in automatic assertion" ;
  rdfs:comment "A type of protein BLAST evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000208 , obo:ECO_0000209 .

obo:ECO_0007588 a rdfs:Class ;
  rdfs:label "high throughput genetic interaction phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of high throughput genetic interaction phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006057 , obo:ECO_0007002 , obo:ECO_0007326 .

obo:ECO_0006066 a rdfs:Class ;
  rdfs:label "fluorescence recovery after photobleaching evidence"@en ;
  rdfs:comment "A type of fluorescence evidence where quantitative information on the diffusion properties of a sample is produced from the measurement of diffusion of fluorescent probes over time into an area that has been photobleached by a high-intensity laser pulse." ;
  rdfs:subClassOf obo:ECO_0001115 .

obo:ECO_0007519 a rdfs:Class ;
  rdfs:label "methylation interference footprinting evidence used in automatic assertion" ;
  rdfs:comment "A type of methylation interference footprinting evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001827 , obo:ECO_0007505 .

obo:ECO_0007249 a rdfs:Class ;
  rdfs:label "nano-cap analysis of gene expression evidence used in automatic assertion" ;
  rdfs:comment "A type of nano-cap analysis of gene expression evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000310 , obo:ECO_0007303 .

obo:OBI_0600024 a rdfs:Class ;
  rdfs:label "maintaining cell culture"@en ;
  rdfs:comment "a protocol application in which cells are kept alive in a defined environment outside of an organism.  part of cell_culturing"@en ;
  rdfs:subClassOf obo:OBI_0000838 .

obo:ECO_0001072 a rdfs:Class ;
  rdfs:label "in vitro translation assay evidence" ;
  rdfs:comment "A type of translation assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001561 .

obo:OBI_0000443 a rdfs:Class ;
  rdfs:label "analyte assay" ;
  rdfs:comment "An assay with the objective to capture information about the presence, concentration, or amount of an analyte in an evaluant." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0006341 a rdfs:Class ;
  rdfs:label "computational structure modeling evidence" ;
  rdfs:comment "A type of structure determination evidence in which a computational tool is used to generate a model of structure(s) for a molecule." ;
  rdfs:subClassOf obo:ECO_0005031 , obo:ECO_0007672 .

obo:ECO_0007450 a rdfs:Class ;
  rdfs:label "fluorescence evidence used in automatic assertion" ;
  rdfs:comment "A type of fluorescence evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001115 , obo:ECO_0007307 .

obo:ECO_0008166 a rdfs:Class ;
  rdfs:label "protein expression-based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of protein expression-based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007309 , obo:ECO_0008078 .

obo:GO_0034249 a rdfs:Class ;
  rdfs:label "negative regulation of cellular amide metabolic process" ;
  rdfs:subClassOf obo:GO_0031324 , obo:GO_0034248 , obo:GO_0051172 , obo:GO_0065007 .

obo:ECO_0007344 a rdfs:Class ;
  rdfs:label "anion-exchange chromatography evidence used in automatic assertion" ;
  rdfs:comment "A type of anion-exchange chromatography evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001010 , obo:ECO_0007681 .

obo:ECO_0008184 a rdfs:Class ;
  rdfs:label "proximity-dependent biotin identification evidence" ;
  rdfs:comment "A type of protein proximity labeling assay evidence in which a mutant of a biotin protein ligase is fused to a protein of interest such that when other proteins enter the proximity of the protein of interest they are biotinylated. Biotinylated proteins are then isolated and identified, often by mass spectrometry." ;
  rdfs:subClassOf obo:ECO_0008183 .

obo:GO_0030308 a rdfs:Class ;
  rdfs:label "negative regulation of cell growth" ;
  rdfs:subClassOf obo:GO_0001558 , obo:GO_0045926 , obo:GO_0048523 , obo:GO_0065007 .

obo:ECO_0001189 a rdfs:Class ;
  rdfs:label "immunohistochemistry evidence used in manual assertion" ;
  rdfs:comment "A type of immunohistochemistry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001055 .

obo:ECO_0006319 a rdfs:Class ;
  rdfs:label "cell aggregation evidence used in manual assertion" ;
  rdfs:comment "A type of cell aggregation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006269 , obo:ECO_0007695 .

obo:OBI_0000989 a rdfs:Class ;
  rdfs:label "PCR instrument" ;
  rdfs:comment "A device that is used to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence." ;
  rdfs:subClassOf obo:OBI_0400116 .

obo:ECO_0008190 a rdfs:Class ;
  rdfs:label "proximity-dependent biotin identification evidence used in manual assertion" ;
  rdfs:comment "A type of proximity-dependent biotin identification evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008184 , obo:ECO_0008188 .

obo:ECO_0006396 a rdfs:Class ;
  rdfs:label "self-reported ancestry evidence used in manual assertion" ;
  rdfs:comment "A type of self-reported ancestry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006157 , obo:ECO_0006395 .

obo:GO_0019538 a rdfs:Class ;
  rdfs:label "protein metabolic process" ;
  rdfs:subClassOf obo:GO_0043170 , obo:GO_0044238 , obo:GO_1901564 .

obo:ECO_0000315 a rdfs:Class ;
  rdfs:label "mutant phenotype evidence used in manual assertion" ;
  rdfs:comment "A type of mutant phenotype evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/imp-inferred-mutant-phenotype> ;
  rdfs:subClassOf obo:ECO_0000015 , obo:ECO_0007634 .

obo:ECO_0000076 a rdfs:Class ;
  rdfs:label "far-Western blotting evidence" ;
  rdfs:comment "A type of physical interaction evidence that is based on detection of protein-protein interactions by separation of target proteins by SDS-PAGE which are blotted to a membrane, followed by denaturation and renaturation, probing with purified bait proteins, and detection of the target-bait complexes." ;
  rdfs:subClassOf obo:ECO_0000021 .

obo:ECO_0000366 a rdfs:Class ;
  rdfs:label "evidence based on logical inference from automatic annotation used in automatic assertion" ;
  rdfs:comment "A type of evidence based on logical inference from an automatically curated annotation that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000363 .

obo:GO_0046700 a rdfs:Class ;
  rdfs:label "heterocycle catabolic process" ;
  rdfs:subClassOf obo:GO_0044248 , obo:GO_0046483 .

obo:ECO_0008088 a rdfs:Class ;
  rdfs:label "antibody-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007373 , obo:ECO_0008039 .

obo:ECO_0007182 a rdfs:Class ;
  rdfs:label "macropatch voltage clamp recording evidence used in manual assertion" ;
  rdfs:comment "A type of macropatch voltage clamp recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005584 , obo:ECO_0006006 .

obo:ECO_0007577 a rdfs:Class ;
  rdfs:label "protein-oligonucleotide microarray binding evidence used in automatic assertion" ;
  rdfs:comment "A type of protein-oligonucleotide microarray binding evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001601 , obo:ECO_0007373 , obo:ECO_0007505 .

obo:ECO_0008105 a rdfs:Class ;
  rdfs:label "antibody B123.2-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody B123.2-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008048 , obo:ECO_0008087 .

obo:ECO_0006085 a rdfs:Class ;
  rdfs:label "protein:ion binding evidence used in manual assertion" ;
  rdfs:comment "A type of protein binding evidence that is used in a manual assertion resulting from a metal ion binding to a protein at a specific binding site." ;
  rdfs:subClassOf obo:ECO_0000142 , obo:ECO_0006076 .

obo:OBI_0400140 a rdfs:Class ;
  rdfs:label "gel tank" ;
  rdfs:comment "A device which holds a gel and running buffers to allow electrophoresis to be performed. A gel tank has the function to contain and to control the contained environment and transfer energy from an energy supply through the running buffers to the gel matrix and the material with charged molecules in an electric field across a porous matrix or medium with the objective to separate the charged molecules." ;
  rdfs:subClassOf obo:OBI_0002089 .

obo:GO_0031047 a rdfs:Class ;
  rdfs:label "gene silencing by RNA" ;
  rdfs:subClassOf obo:GO_0010629 .

obo:ECO_0001814 a rdfs:Class ;
  rdfs:label "methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence" ;
  rdfs:comment "A type of nucleic acid binding evidence where the synthetic molecule methidiumpropyl-EDTA (MPE) is used to cleave ligand-protected DNA, followed by analysis of the restriction fragments to generate a footprint (i.e. size and location) of small molecule binding sites on the DNA." ;
  rdfs:subClassOf obo:ECO_0000136 .

obo:ECO_0006356 a rdfs:Class ;
  rdfs:label "thioflavin T based fluorescence spectroscopy evidence used in manual assertion" ;
  rdfs:comment "A type of thioflavin T based fluorescence spectroscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001249 , obo:ECO_0006333 .

obo:ECO_0006298 a rdfs:Class ;
  rdfs:label "GAL4-VP16 functional complementation evidence used in automatic assertion" ;
  rdfs:comment "A type of GAL4-VP16 functional complementation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006258 , obo:ECO_0006294 .

obo:ECO_0001256 a rdfs:Class ;
  rdfs:label "imaging assay evidence used in manual assertion" ;
  rdfs:comment "A type of imaging assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0000324 .

obo:ECO_0000210 a rdfs:Class ;
  rdfs:label "nucleotide BLAST evidence used in automatic assertion" ;
  rdfs:comment "A type of nucleotide BLAST evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000207 , obo:ECO_0000209 .

obo:GO_0051254 a rdfs:Class ;
  rdfs:label "positive regulation of RNA metabolic process" ;
  rdfs:subClassOf obo:GO_0010604 , obo:GO_0045935 , obo:GO_0051252 , obo:GO_0065007 .

obo:ECO_0007354 a rdfs:Class ;
  rdfs:label "nucleotide analog incorporation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of nucleotide analog incorporation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001107 , obo:ECO_0007776 .

obo:ECO_0006137 a rdfs:Class ;
  rdfs:label "quantum mechanics/molecular mechanics simulation evidence used in manual assertion" ;
  rdfs:comment "A type of quantum mechanics/molecular mechanics simulation evidence that is used in manual assertion." ;
  rdfs:subClassOf obo:ECO_0006135 .

obo:ECO_0006179 a rdfs:Class ;
  rdfs:label "far-UV circular dichroism evidence" ;
  rdfs:comment "A type of circular dichroism evidence whose spectrum is in the range of 190-230 nm, providing information mainly about amide bonds and through which the relative proportion of secondary structure elements of a protein can be estimated." ;
  rdfs:subClassOf obo:ECO_0006177 .

obo:BFO_0000002 a rdfs:Class ;
  rdfs:label "continuant"@en ;
  rdfs:comment "An entity that exists in full at any time in which it exists at all, persists through time while maintaining its identity and has no temporal parts."@en ;
  rdfs:subClassOf obo:BFO_0000001 .

obo:ECO_0008115 a rdfs:Class ;
  rdfs:label "antibody BB7.2-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody BB7.2-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008053 , obo:ECO_0008087 .

obo:ECO_0001227 a rdfs:Class ;
  rdfs:label "luminescence-based mammalian interactome mapping assay evidence used in manual assertion" ;
  rdfs:comment "A type of LUMIER assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000353 , obo:ECO_0001093 .

obo:ECO_0001036 a rdfs:Class ;
  rdfs:label "cytochrome C release assay evidence" ;
  rdfs:comment "A type of apoptotic assay evidence resulting from the identification of an accumulation of cytochrome C in the cytoplasm after its release from the mitochondria, an early event in apoptosis." ;
  rdfs:subClassOf obo:ECO_0005034 .

obo:GO_0051049 a rdfs:Class ;
  rdfs:label "regulation of transport" ;
  rdfs:subClassOf obo:GO_0032879 , obo:GO_0065007 .

obo:GO_0005216 a rdfs:Class ;
  rdfs:label "ion channel activity" ;
  rdfs:comment "Enables the facilitated diffusion of an ion (by an energy-independent process) by passage through a transmembrane aqueous pore or channel without evidence for a carrier-mediated mechanism. May be either selective (it enables passage of a specific ion only) or non-selective (it enables passage of two or more ions of same charge but different size)." ;
  rdfs:subClassOf obo:GO_0009987 , obo:GO_0015075 , obo:GO_0015267 , obo:GO_0015318 .

obo:ECO_000157 a rdfs:Class ;
  rdfs:label "colony diameter phenotype evidence used in manual assertion" ;
  rdfs:comment "A type of colony diameter phenotype evidence that is used in manual assertion." ;
  rdfs:subClassOf obo:ECO_0001571 , obo:ECO_0007082 .

obo:ECO_0006284 a rdfs:Class ;
  rdfs:label "electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence used in automatic assertion" ;
  rdfs:comment "A type of electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006251 , obo:ECO_0007433 .

obo:OBI_0003122 a rdfs:Class ;
  rdfs:label "3D cell structure determination assay" ;
  rdfs:comment "A 3D structure determination assay that determines the 3-dimensional configuration of a cell." ;
  rdfs:subClassOf obo:OBI_0001977 , obo:OBI_0600045 .

obo:GO_0009891 a rdfs:Class ;
  rdfs:label "positive regulation of biosynthetic process" ;
  rdfs:subClassOf obo:GO_0009889 , obo:GO_0009893 , obo:GO_0065007 .

obo:ECO_0008156 a rdfs:Class ;
  rdfs:label "antibody SPVL3-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody SPVL3-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008073 , obo:ECO_0008088 .

obo:ECO_0006198 a rdfs:Class ;
  rdfs:label "proton-based nuclear magnetic resonance evidence used in manual assertion" ;
  rdfs:comment "A type of proton-based nuclear magnetic resonance evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006165 , obo:ECO_0006176 .

obo:GO_0009611 a rdfs:Class ;
  rdfs:label "response to wounding" ;
  rdfs:subClassOf obo:GO_0006950 .

obo:ECO_0001196 a rdfs:Class ;
  rdfs:label "in vitro deubiquitination assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro deubiquitination assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001059 .

obo:ECO_0006399 a rdfs:Class ;
  rdfs:label "antibody Tu36-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A  type of antibody Tu36-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006398 , obo:ECO_0008088 .

obo:GO_2000678 a rdfs:Class ;
  rdfs:label "negative regulation of transcription regulatory region DNA binding" ;
  rdfs:subClassOf obo:GO_0043392 , obo:GO_0048519 , obo:GO_0065007 , obo:GO_2000677 .

obo:OBI_0000011 a rdfs:Class ;
  rdfs:label "planned process"@en ;
  rdfs:comment "A process that realizes a plan which is the concretization of a plan specification."@en ;
  rdfs:subClassOf obo:BFO_0000015 .

obo:OBI_0001931 a rdfs:Class ;
  rdfs:label "scalar value specification" ;
  rdfs:comment "A value specification that consists of two parts: a numeral and a unit label" ;
  rdfs:subClassOf obo:OBI_0001933 .

obo:ECO_0007111 a rdfs:Class ;
  rdfs:label "in vitro recombinant protein transcription reconstitution assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro recombinant protein transcription reconstitution assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000152 , obo:ECO_0000314 .

obo:ECO_0007379 a rdfs:Class ;
  rdfs:label "deletion mutation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of deletion mutation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001038 , obo:ECO_0007325 .

obo:ECO_0006003 a rdfs:Class ;
  rdfs:label "electron microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of electron microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001232 , obo:ECO_0005033 .

obo:ECO_0005566 a rdfs:Class ;
  rdfs:label "sustained current evidence" ;
  rdfs:comment "A type of electrophysiology assay evidence that involves the measurement of the current that persists after the fast and slow inactivation of the analyzed channels." ;
  rdfs:subClassOf obo:ECO_0000164 .

obo:ECO_0008189 a rdfs:Class ;
  rdfs:label "protein proximity labeling assay evidence used in automatic assertion" ;
  rdfs:comment "A type of protein proximity labeling assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007307 , obo:ECO_0008183 .

obo:ECO_0007337 a rdfs:Class ;
  rdfs:label "7-aminoactinomycin staining evidence used in automatic assertion" ;
  rdfs:comment "A type of 7-aminoactinomycin staining evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001005 , obo:ECO_0007798 .

obo:ECO_0005509 a rdfs:Class ;
  rdfs:label "insertion mutation phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence resulting from a mutation in which there is an insertion of one or more contiguous nucleotides." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:GO_2001252 a rdfs:Class ;
  rdfs:label "positive regulation of chromosome organization" ;
  rdfs:subClassOf obo:GO_0010638 , obo:GO_0033044 , obo:GO_0065007 .

obo:OBI_0001985 a rdfs:Class ;
  rdfs:label "microarray assay" ;
  rdfs:comment "An analyte assay where binding of the analyte to immobilized matrix is measured." ;
  rdfs:subClassOf obo:OBI_0000443 .

obo:ECO_0001025 a rdfs:Class ;
  rdfs:label "co-electrophoresis evidence" ;
  rdfs:comment "A type of gel electrophoresis evidence resulting from the electrophoresis of two substances together, allowing for the characterization of ligand/nucleic acid binding interactions." ;
  rdfs:subClassOf obo:ECO_0000337 .

obo:ECO_0007212 a rdfs:Class ;
  rdfs:label "beta-glucuronidase fusion protein localization evidence used in automatic assertion" ;
  rdfs:comment "A type of beta-glucuronidase fusion protein localization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000130 , obo:ECO_0007808 .

obo:ECO_0006295 a rdfs:Class ;
  rdfs:label "beta galactosidase functional complementation evidence used in manual assertion" ;
  rdfs:comment "A type of beta galactosidase functional complementation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006257 , obo:ECO_0006293 .

obo:ECO_0007235 a rdfs:Class ;
  rdfs:label "5C evidence used in automatic assertion" ;
  rdfs:comment "A type of 5C evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000235 , obo:ECO_0007822 .

obo:ECO_0001556 a rdfs:Class ;
  rdfs:label "phosphatase assay evidence" ;
  rdfs:comment "A type of direct assay evidence where the removal of phosphatase groups from a protein is detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007572 a rdfs:Class ;
  rdfs:label "ribohomopolymer binding assay evidence used in automatic assertion" ;
  rdfs:comment "A type of ribohomopolymer binding assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000138 , obo:ECO_0007505 .

obo:ECO_0007460 a rdfs:Class ;
  rdfs:label "xenotransplantation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of xenotransplantation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001137 , obo:ECO_0007810 .

obo:ECO_0006302 a rdfs:Class ;
  rdfs:label "protein thermal shift assay evidence used in automatic assertion" ;
  rdfs:comment "A type of protein thermal shift assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006260 , obo:ECO_0006318 .

obo:ECO_0006071 a rdfs:Class ;
  rdfs:label "immuno-labelling electron microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of electron microscopy evidence used in a manual assertion resulting from the use of antibodies to identify the localization of antigens in cells and tissues." ;
  rdfs:subClassOf obo:ECO_0006003 , obo:ECO_0006070 .

obo:ECO_0001257 a rdfs:Class ;
  rdfs:label "restriction fragment detection evidence used in manual assertion" ;
  rdfs:comment "A type of restriction fragment detection evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001124 .

obo:ECO_0005033 a rdfs:Class ;
  rdfs:label "electron microscopy evidence" ;
  rdfs:comment "A type of microscopy evidence and structure determination evidence resulting from an electron beam utilized to create an image." ;
  rdfs:subClassOf obo:ECO_0001098 , obo:ECO_0005031 .

obo:ECO_0000148 a rdfs:Class ;
  rdfs:label "obsolete in vitro binding evidence" .

obo:ECO_0008178 a rdfs:Class ;
  rdfs:label "MHC-specific blocking based MHC restriction evidence used in automatic assertion" ;
  rdfs:comment "A type of MHC-specific blocking based MHC restriction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007373 , obo:ECO_0008084 .

obo:ECO_0005805 a rdfs:Class ;
  rdfs:label "yeast 2-hybrid evidence used in manual assertion" ;
  rdfs:comment "A type of yeast 2-hybrid evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000068 , obo:ECO_0006077 .

obo:GO_0009605 a rdfs:Class ;
  rdfs:label "response to external stimulus" ;
  rdfs:subClassOf obo:GO_0050896 .

obo:GO_0120032 a rdfs:Class ;
  rdfs:label "regulation of plasma membrane bounded cell projection assembly" ;
  rdfs:subClassOf obo:GO_0060491 , obo:GO_0065007 , obo:GO_0120035 .

obo:ECO_0001159 a rdfs:Class ;
  rdfs:label "carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion" ;
  rdfs:comment "A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001022 .

obo:ECO_0000324 a rdfs:Class ;
  rdfs:label "imaging assay evidence" ;
  rdfs:comment "A type of direct assay evidence in which an image is created and analyzed." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:GO_0006412 a rdfs:Class ;
  rdfs:label "translation" ;
  rdfs:subClassOf obo:GO_0034645 , obo:GO_0043043 , obo:GO_0044267 .

obo:ECO_0006036 a rdfs:Class ;
  rdfs:label "genetically encoded fluorescent sensor evidence" ;
  rdfs:comment "A type of fluorescent sensor evidence that is based on direct, quantitative measurement of some cellular property using a genetically encoded fluorescent sensor." ;
  rdfs:subClassOf obo:ECO_0006034 .

obo:ECO_0007503 a rdfs:Class ;
  rdfs:label "immunoprecipitation evidence used in automatic assertion" ;
  rdfs:comment "A type of immunoprecipitation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000085 , obo:ECO_0007373 .

obo:OBI_0001672 a rdfs:Class ;
  rdfs:label "gene knock-down assay" ;
  rdfs:comment "An assay which transiently disrupts gene transcripts by expressing antisense RNA constructs or delivering RNA interfering molecules in cells." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0006067 a rdfs:Class ;
  rdfs:label "fluorescence recovery after photobleaching evidence used in manual assertion"@en ;
  rdfs:comment "A type of fluorescence evidence that is used in a manual assertion where quantitative information on the diffusion properties of a sample is produced from the measurement of diffusion of fluorescent probes over time into an area that has been photobleached by a high-intensity laser pulse." ;
  rdfs:subClassOf obo:ECO_0001249 , obo:ECO_0006066 .

obo:IAO_0000033 a rdfs:Class ;
  rdfs:label "directive information entity"@en ;
  rdfs:comment "An information content entity whose concretizations indicate to their bearer how to realize them in a process."@en ;
  rdfs:subClassOf obo:IAO_0000030 .

obo:ECO_0000019 a rdfs:Class ;
  rdfs:label "RNAi evidence" ;
  rdfs:comment "A type of mutant phenotype evidence where an RNA construct is introduced into a cell and the expression of the gene bearing its complementary sequence is suppressed." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0001007 a rdfs:Class ;
  rdfs:label "adoptive cell transfer evidence" ;
  rdfs:comment "A type of ex vivo assay evidence derived by ex vivo selection of tumor-reactive lymphocytes, and their activation and numerical expansion before re-infusion to the autologous tumor-bearing host." ;
  rdfs:subClassOf obo:ECO_0005502 .

obo:ECO_0008082 a rdfs:Class ;
  rdfs:label "inference of MHC restriction based on shared MHC expression" ;
  rdfs:comment "A type of inferential evidence that employs the association of specific MHC molecule expression by a subject population with a T cell response to infer that T cells can recognize the ligand when presented by that MHC molecule." ;
  rdfs:subClassOf obo:ECO_0000361 .

obo:ECO_0001062 a rdfs:Class ;
  rdfs:label "in vitro demethylation assay evidence" ;
  rdfs:comment "A type of demethylation assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001550 .

obo:ECO_0005596 a rdfs:Class ;
  rdfs:label "immunoperoxidase immunolocalization electron microscopy evidence" ;
  rdfs:comment "A type of immunoperoxidase immunolocalization evidence where electron microscopy technique is used for enzymatic detection of a peroxidase tagged antibody." ;
  rdfs:subClassOf obo:ECO_0005033 , obo:ECO_0005594 .

obo:OBI_0600051 a rdfs:Class ;
  rdfs:label "gradient separation"@en ;
  rdfs:comment "a protocol application that uses different concentrations of materials in a defined order to create a gradient to facilitate the separation of an input material into its components with specific qualities"@en ;
  rdfs:subClassOf obo:OBI_0600014 .

obo:OBI_0000925 a rdfs:Class ;
  rdfs:label "infectious agent"@en ;
  rdfs:comment "is a material entity bearing the disposition to infect an organism"@en .

obo:RO_0000053 a rdf:Property ;
  rdfs:label "bearer of"@en ;
  rdfs:comment "a relation between an independent continuant (the bearer) and a specifically dependent continuant (the dependent), in which the dependent specifically depends on the bearer for its existence"@en ;
  rdfs:range obo:BFO_0000020 .

obo:ECO_0000028 a rdfs:Class ;
  rdfs:label "motif similarity evidence" ;
  rdfs:comment "A type of match to sequence model evidence that is based on the presence of a recognized domain or motif in a gene product's (usually protein) primary sequence." ;
  rdfs:subClassOf obo:ECO_0000202 .

obo:ECO_0006060 a rdfs:Class ;
  rdfs:label "high throughput cell biology evidence used in automatic assertion" ;
  rdfs:comment "A type of high throughput evidence used in an automatic assertion where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel." ;
  rdfs:subClassOf obo:ECO_0006057 , obo:ECO_0006058 .

obo:ECO_0007808 a rdfs:Class ;
  rdfs:label "fusion protein localization evidence used in automatic assertion" ;
  rdfs:comment "A type of fusion protein localization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000124 , obo:ECO_0007807 .

obo:ECO_0001095 a rdfs:Class ;
  rdfs:label "mammalian 2-hybrid assay evidence" ;
  rdfs:comment "A type of bait-prey hybrid interaction evidence resulting from the analysis of proteins of interest attached to two portions of the transcriptional activator, and in interaction bring the two portions together to increase expression of the reporter gene." ;
  rdfs:subClassOf obo:ECO_0000025 .

obo:ECO_0007007 a rdfs:Class ;
  rdfs:label "high throughput expression pattern evidence used in manual assertion" ;
  rdfs:comment "A type of high throughput evidence that is used in a manual assertion derived from the high throughput characterization of gene expression." ;
  rdfs:subClassOf obo:ECO_0000270 , obo:ECO_0006056 , obo:ECO_0007006 .

obo:ECO_0005661 a rdfs:Class ;
  rdfs:label "rapid amplification of cDNA ends polymerase chain reaction evidence used in manual assertion" ;
  rdfs:comment "A type of rapid amplification of cDNA ends polymerase chain reaction evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001820 .

obo:ECO_0000293 a rdfs:Class ;
  rdfs:label "systematic evolution of ligands by exponential amplification evidence" ;
  rdfs:comment "A type of evidence arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand." ;
  rdfs:subClassOf obo:ECO_0000021 .

obo:ECO_0000174 a rdfs:Class ;
  rdfs:label "mutant physiological response evidence" ;
  rdfs:comment "A type of mutant phenotype evidence in which the physiological response of a mutant when presented with an external stimulus is determined." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:OBI_0000201 a rdfs:Class ;
  rdfs:label "radioactivity detection" ;
  rdfs:comment "An assay that measures the amount of radiation in the radioactive spectrum (alpha, beta or gamma rays) emitted from an input material." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007033 a rdfs:Class ;
  rdfs:label "neutralization test assay evidence" ;
  rdfs:comment "A type of immunogical assay evidence that results in detection of specific neutralizing antibodies in a sample." ;
  rdfs:subClassOf obo:ECO_0000040 .

obo:CL_0000540 a rdfs:Class ;
  rdfs:label "neuron" ;
  rdfs:comment "The basic cellular unit of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the nervous system." ;
  rdfs:subClassOf obo:CL_0002319 .

obo:GO_0034708 a rdfs:Class ;
  rdfs:label "methyltransferase complex" ;
  rdfs:subClassOf obo:GO_1990234 .

obo:OBI_0002171 a rdfs:Class ;
  rdfs:label "dot blot assay" ;
  rdfs:comment "An analyte assay that detects molecules in a mixture dotted on a membrane using DNA probes or antibodies." ;
  rdfs:subClassOf obo:OBI_0000443 .

obo:ECO_0007557 a rdfs:Class ;
  rdfs:label "morpholino experiment evidence used in automatic assertion" ;
  rdfs:comment "A type of morpholino experiment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000292 , obo:ECO_0007325 .

obo:OBI_0000953 a rdfs:Class ;
  rdfs:label "processed specimen" ;
  rdfs:comment "A specimen that has been intentionally physically modified." ;
  rdfs:subClassOf obo:OBI_0000047 , obo:OBI_0100051 .

obo:ECO_0000304 a rdfs:Class ;
  rdfs:label "author statement supported by traceable reference used in manual assertion" ;
  rdfs:comment "A type of author statement supported by traceable reference that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/tas-traceable-author-statement> ;
  rdfs:subClassOf obo:ECO_0000033 , obo:ECO_0000302 .

obo:ECO_0001825 a rdfs:Class ;
  rdfs:label "isothermal titration calorimetry evidence" ;
  rdfs:comment "A type of affinity evidence where the absorbed or released heat of a biomolecular binding event is directly measured in a reference cell and sample cell after the addition of a ligand using a microcalorimeter for a complete thermodynamic profile of the molecular interaction." ;
  rdfs:subClassOf obo:ECO_0000023 .

obo:ECO_0001137 a rdfs:Class ;
  rdfs:label "xenotransplantation phenotypic evidence" ;
  rdfs:comment "A type of anatomical perturbation phenotypic evidence resulting from the transplantation, implantation, or infusion of live cells, tissues, or organs between individuals of different species." ;
  rdfs:subClassOf obo:ECO_0000240 .

obo:ECO_0001018 a rdfs:Class ;
  rdfs:label "bromodeoxyuridine incorporation assay evidence" ;
  rdfs:comment "A type of nucleotide analog incorporation evidence resulting from the incorporation of bromodeoxyuridine (BrdU) as a thymidine analog into nuclear DNA, resulting in a label that can be tracked using antibody probes." ;
  rdfs:subClassOf obo:ECO_0001107 .

obo:ECO_0007348 a rdfs:Class ;
  rdfs:label "immunological assay evidence used in automatic assertion" ;
  rdfs:comment "A type of immunological assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000040 , obo:ECO_0007384 .

obo:ECO_0006243 a rdfs:Class ;
  rdfs:label "polyadenylated transcript 3'-end-sequencing evidence used in manual assertion" ;
  rdfs:comment "A type of polyadenylated transcript 3'-end-sequencing evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006068 , obo:ECO_0006239 .

obo:GO_1904796 a rdfs:Class ;
  rdfs:label "regulation of core promoter binding" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_2000677 .

obo:GO_0007568 a rdfs:Class ;
  rdfs:label "aging" ;
  rdfs:subClassOf obo:GO_0032502 .

obo:ECO_0007561 a rdfs:Class ;
  rdfs:label "wide-field fluorescence microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of wide-field fluorescence microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005598 , obo:ECO_0007491 .

obo:ECO_0008093 a rdfs:Class ;
  rdfs:label "antibody 28-14-8S-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody 28-14-8S-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008042 , obo:ECO_0008087 .

obo:OBI_0400043 a rdfs:Class ;
  rdfs:label "flow cell" ;
  rdfs:comment "Aparatus in the fluidic subsystem where the sheath and sample meet. Can be one of several types; jet-in-air, quartz cuvette, or a hybrid of the two. The sample flows through the center of a fluid column of sheath fluid in the flow cell." ;
  rdfs:subClassOf obo:OBI_0000967 .

obo:ECO_0007240 a rdfs:Class ;
  rdfs:label "environmental perturbation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of environmental perturbation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000241 , obo:ECO_0007635 .

obo:GO_0032071 a rdfs:Class ;
  rdfs:label "regulation of endodeoxyribonuclease activity" ;
  rdfs:subClassOf obo:GO_0032070 , obo:GO_0065007 .

obo:ECO_0005604 a rdfs:Class ;
  rdfs:label "cross-streak test evidence" ;
  rdfs:comment "A type of cell proliferation assay evidence where the investigated organism or a potential antagonist is inoculated so as to grow along the diameter line of the agar medium in a Petri dish, then, potentially susceptible microbes (usually bacteria) are streaked perpendicularly to the growth line and inhibition of growth of a test microbe in the vicinity of that line suggests that the antagonist secretes one or more diffusible antibiotics." ;
  rdfs:subClassOf obo:ECO_0005007 .

obo:GO_0044267 a rdfs:Class ;
  rdfs:label "cellular protein metabolic process" ;
  rdfs:subClassOf obo:GO_0019538 , obo:GO_0044260 .

obo:ECO_0000223 a rdfs:Class ;
  rdfs:label "454 pyrosequencing evidence" ;
  rdfs:comment "A type of high throughput nucleotide sequencing evidence in which the sequence for a ssDNA is determined by addition of primers and then nucleotides, one base pair at a time (which generate a specific fluorescence), to the target strand." ;
  rdfs:subClassOf obo:ECO_0000221 .

obo:ECO_0001242 a rdfs:Class ;
  rdfs:label "phage display evidence used in manual assertion" ;
  rdfs:comment "A type of phage display evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001108 , obo:ECO_0006076 .

obo:ECO_0007292 a rdfs:Class ;
  rdfs:label "capillary assay evidence used in automatic assertion" ;
  rdfs:comment "A type of capillary assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005609 , obo:ECO_0007780 .

obo:GO_0040013 a rdfs:Class ;
  rdfs:label "negative regulation of locomotion" ;
  rdfs:subClassOf obo:GO_0040012 , obo:GO_0048519 , obo:GO_0065007 .

obo:ECO_0006018 a rdfs:Class ;
  rdfs:label "inference based on individual clinical experience" ;
  rdfs:comment "A type of curator inference that is based on the individual clinical experience of a clinician" ;
  rdfs:subClassOf obo:ECO_0000205 .

obo:ECO_0007192 a rdfs:Class ;
  rdfs:label "motility assay evidence used in manual assertion" ;
  rdfs:comment "A type of motility assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0005803 .

obo:ECO_0000212 a rdfs:Class ;
  rdfs:label "combinatorial evidence" ;
  rdfs:comment "A type of evidence in which at least two distinct types of evidence have been integrated." ;
  rdfs:subClassOf obo:ECO_0000000 .

obo:GO_0016043 a rdfs:Class ;
  rdfs:label "cellular component organization" ;
  rdfs:subClassOf obo:GO_0071840 .

obo:ECO_0007063 a rdfs:Class ;
  rdfs:label "dynamic light scattering assay evidence" ;
  rdfs:comment "A type of light scattering evidence derived by analyzing the fluctuations in the internsity of the scattered light by the particles in motion." ;
  rdfs:subClassOf obo:ECO_0007061 .

obo:OBI_0001476 a rdfs:Class ;
  rdfs:label "phage display binding assay" ;
  rdfs:comment "A binding assay in which a collection of phages expressing a library of different peptides or protein fragnments is used to infect cells, followed by screening for cells that bind a protein of interest, and identifiying the sequence of infecting phages to determine a suitable binding partner." ;
  rdfs:subClassOf obo:OBI_0001146 .

obo:CL_0000236 a rdfs:Class ;
  rdfs:label "B cell" ;
  rdfs:comment "A lymphocyte of B lineage with the phenotype CD19-positive, CD20-positive, and capable of B cell mediated immunity." ;
  rdfs:subClassOf obo:CL_0000542 .

obo:ECO_0008033 a rdfs:Class ;
  rdfs:label "intrinsic disorder prediction evidence used in manual assertion" ;
  rdfs:comment "A type of intrinsic disorder prediction evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007668 , obo:ECO_0008030 .

obo:ECO_0007223 a rdfs:Class ;
  rdfs:label "in vivo assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000178 , obo:ECO_0007307 .

obo:ECO_0007073 a rdfs:Class ;
  rdfs:label "bacterial 2-hybrid assay evidence" ;
  rdfs:comment "A type of bait-prey hybrid interaction evidence that involves the detection of protein-protein interaction by fusing the protein target (the \"bait\") to RNA polymerase and fusing protein or peptide library to be analyzed (the \"prey\") to DNA-binding domain." ;
  rdfs:subClassOf obo:ECO_0000025 .

obo:ECO_0006083 a rdfs:Class ;
  rdfs:label "nucleic acid binding evidence used in manual assertion" ;
  rdfs:comment "A type of affinity evidence that is used in a manual assertion resulting from the binding of a molecule to a nucleic acid." ;
  rdfs:subClassOf obo:ECO_0000136 , obo:ECO_0006075 .

obo:PATO_0001739 a rdfs:Class ;
  rdfs:label "radiation quality" ;
  rdfs:comment "A quality that inheres in an bearer by virtue of how that bearer interacts with radiation." ;
  rdfs:subClassOf obo:PATO_0001018 .

obo:ECO_0007681 a rdfs:Class ;
  rdfs:label "chromatography evidence used in automatic assertion" ;
  rdfs:comment "A type of chromatography evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000325 , obo:ECO_0007297 .

obo:ECO_0005617 a rdfs:Class ;
  rdfs:label "chloramphenicol acetyltransferase reporter gene assay evidence used in manual assertion" ;
  rdfs:comment "A type of chloramphenicol acetyltransferase reporter gene assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000270 , obo:ECO_0001803 .

obo:ECO_0001836 a rdfs:Class ;
  rdfs:label "in situ hybridization evidence" ;
  rdfs:comment "A type of expression pattern evidence in which single-stranded DNA or RNA (probe) are annealed to complementary DNA or RNA in a portion or section of tissue." ;
  rdfs:subClassOf obo:ECO_0000008 .

obo:OBI_0001463 a rdfs:Class ;
  rdfs:label "transcription profiling by array assay" ;
  rdfs:comment "A transcription profiling assay that uses array technology." ;
  rdfs:subClassOf obo:OBI_0000424 .

obo:ECO_0000006 a rdfs:Class ;
  rdfs:label "experimental evidence" ;
  rdfs:comment "A type of evidence that is the output of a scientific procedure performed to make a discovery, test a hypothesis, or demonstrate a known fact." ;
  rdfs:subClassOf obo:ECO_0000000 .

obo:ECO_0007044 a rdfs:Class ;
  rdfs:label "transmission electron microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of transmission electron microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006003 , obo:ECO_0007043 .

obo:ECO_0007583 a rdfs:Class ;
  rdfs:label "epistatic interaction phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of epistatic interaction phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000056 , obo:ECO_0007326 .

obo:ECO_0007637 a rdfs:Class ;
  rdfs:label "curator inference from encyclopedia" ;
  rdfs:comment "A type of curator inference from published work where the reference is to an entry in a compendium that provides summarized information on a subject." ;
  rdfs:subClassOf obo:ECO_0007647 .

obo:ECO_0000075 a rdfs:Class ;
  rdfs:label "gene expression similarity evidence" ;
  rdfs:comment "A type of phenotypic similarity evidence that is based on the categorization of genes by the similarity of expression profiles." ;
  rdfs:subClassOf obo:ECO_0000057 .

obo:ECO_0001567 a rdfs:Class ;
  rdfs:label "quantitative reverse transcription polymerase chain reaction evidence used in manual assertion" ;
  rdfs:comment "A type of quantitative reverse transcription polymerase chain reaction evidence used in manual assertion." ;
  rdfs:subClassOf obo:ECO_0001566 , obo:ECO_0001808 .

obo:OBI_0001365 a rdfs:Class ;
  rdfs:label "cellular process design" ;
  rdfs:comment "A study design that aims to study the processes that are carried out at the cellular level, but are not necessarily restricted to a single cell. For example, cell communication occurs among more than one cell, but occurs at the cellular level." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:ECO_0007304 a rdfs:Class ;
  rdfs:label "Nimblegen array evidence used in automatic assertion" ;
  rdfs:comment "A type of Nimblegen array evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000105 , obo:ECO_0005630 .

obo:ECO_0001246 a rdfs:Class ;
  rdfs:label "physical examination evidence used in manual assertion" ;
  rdfs:comment "A type of physical examination evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001111 .

obo:ECO_0005533 a rdfs:Class ;
  rdfs:label "phylogenetic footprinting evidence" ;
  rdfs:comment "A type of motif discovery evidence based on a set of algorithms that infer motifs in non-coding DNA sequences from the conservation of functional regulatory sites through evolution at a higher rate than their local surroundings for the identification of an evolutionary 'footprint'." ;
  rdfs:subClassOf obo:ECO_0005531 .

obo:GO_0048532 a rdfs:Class ;
  rdfs:label "anatomical structure arrangement" ;
  rdfs:subClassOf obo:GO_0032502 .

obo:ECO_0000209 a rdfs:Class ;
  rdfs:label "BLAST evidence used in automatic assertion" ;
  rdfs:comment "A type of BLAST evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000206 , obo:ECO_0005561 .

obo:OBI_0002622 a rdfs:Class ;
  rdfs:label "in vitro transcription reconstitution assay" ;
  rdfs:comment "A reconstitution assay in which RNA synthesis is investigated using RNA polymerase II transcription systems replicated in vitro from their base components." ;
  rdfs:subClassOf obo:OBI_0002621 .

obo:GO_1903792 a rdfs:Class ;
  rdfs:label "negative regulation of anion transport" ;
  rdfs:subClassOf obo:GO_0043271 , obo:GO_0044070 , obo:GO_0065007 .

obo:ECO_0007270 a rdfs:Class ;
  rdfs:label "repeat expansion mutation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of repeat expansion mutation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005513 , obo:ECO_0007325 .

obo:GO_0008156 a rdfs:Class ;
  rdfs:label "negative regulation of DNA replication" ;
  rdfs:subClassOf obo:GO_0006275 , obo:GO_0065007 , obo:GO_2000113 .

obo:ECO_0005599 a rdfs:Class ;
  rdfs:label "immunofluorescence wide-field microscopy evidence" ;
  rdfs:comment "A type of wide-field fluorescence microscopy evidence where fluorescently tagged antibodies are imaged that are used to bind to their antigens." ;
  rdfs:subClassOf obo:ECO_0000007 , obo:ECO_0005598 .

obo:GO_0070201 a rdfs:Class ;
  rdfs:label "regulation of establishment of protein localization" ;
  rdfs:subClassOf obo:GO_0032880 , obo:GO_0065007 .

obo:ECO_0000122 a rdfs:Class ;
  rdfs:label "protein localization evidence" ;
  rdfs:comment "A type of localization evidence where sub-cellular localization of a protein is determined." ;
  rdfs:subClassOf obo:ECO_0001533 .

obo:ECO_0007740 a rdfs:Class ;
  rdfs:label "small molecule detection assay evidence used in manual assertion" ;
  rdfs:comment "A type of small molecule detection assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001522 .

obo:OBI_0001683 a rdfs:Class ;
  rdfs:label "chromosome organization assay by fluorescence in-situ hybridization" ;
  rdfs:comment "An in-situ hybridization assay that uses fluorescence to detect chromosomal integrity" ;
  rdfs:subClassOf obo:OBI_0001686 .

obo:ECO_0007084 a rdfs:Class ;
  rdfs:label "zone of inhibition evidence used in manual assertion" ;
  rdfs:comment "A type of zone of inhibition evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007083 , obo:ECO_0007699 .

obo:ECO_0007162 a rdfs:Class ;
  rdfs:label "silent mutation evidence used in manual assertion" ;
  rdfs:comment "A type of silent mutation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001247 , obo:ECO_0005508 .

obo:ECO_0007059 a rdfs:Class ;
  rdfs:label "confocal laser scanning microscopy evidence" ;
  rdfs:comment "A type of confocal microscopy evidence that results in an image that is built up pixel-by-pixel by collecting the emitted photons from the fluorophores in the sample by passing a laser beam through a light source aperture which is then focused by an objective lens into a small area on the surface of the sample." ;
  rdfs:subClassOf obo:ECO_0005587 .

obo:OBI_0000711 a rdfs:Class ;
  rdfs:label "library preparation" ;
  rdfs:comment "is a process which results in the creation of a library from fragments of DNA using cloning vectors or oligonucleotides with the role of adaptors." ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0008167 a rdfs:Class ;
  rdfs:label "specific MHC protein expression-based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of specific MHC protein expression-based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008079 , obo:ECO_0008165 .

obo:OBI_0001147 a rdfs:Class ;
  rdfs:label "cell culture expansion"@en ;
  rdfs:comment "a process that results in the increase of cell numbers"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0000086 a rdfs:Class ;
  rdfs:label "amplification of intermethylated sites evidence" ;
  rdfs:comment "A type of in vitro methylation assay evidence resulting from a genome-wide estimate of DNA methylation by differential enzymatic digestion of genomic DNA with methylation-sensitive and methylation-insensitive isoschizomers, followed by restrained PCR amplification of sequences methylated at both ends and resolving the PCR products in a denaturing polyacrylamide-sequencing gel to generate fingerprints that consist of multiple anonymous bands that represent the DNA methylome of the cell." ;
  rdfs:subClassOf obo:ECO_0001065 .

obo:ECO_0006094 a rdfs:Class ;
  rdfs:label "suppressor/enhancer interaction phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of suppressor/enhancer interaction phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000052 , obo:ECO_0000316 .

obo:OBI_9999994 a rdfs:Class ;
  rdfs:label "chromium release assay" ;
  rdfs:comment "An in vitro cell killing assay in which radioactive chromium is absorbed by cells and released into supernatant when the cells die. The amount of radioactivity measured in the supernatant is a proxy for the number of cells that have died." ;
  rdfs:subClassOf obo:OBI_0000903 .

obo:GO_0044249 a rdfs:Class ;
  rdfs:label "cellular biosynthetic process" ;
  rdfs:subClassOf obo:GO_0009058 , obo:GO_0044237 .

obo:ECO_0007722 a rdfs:Class ;
  rdfs:label "reconstitution assay evidence used in manual assertion" ;
  rdfs:comment "A type of reconstitution assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000003 , obo:ECO_0000314 .

obo:IAO_0000064 a rdfs:Class ;
  rdfs:label "algorithm"@en ;
  rdfs:comment "A plan specification which describes the inputs and output of mathematical functions as well as workflow of execution for achieving an predefined objective. Algorithms are realized usually by means of implementation as computer programs for execution by automata."@en ;
  rdfs:subClassOf obo:IAO_0000104 .

obo:ECO_0008030 a rdfs:Class ;
  rdfs:label "intrinsic disorder prediction evidence" ;
  rdfs:comment "A type of computational evidence where one or more computational tools are used to predict regions of intrinsic disorder in a protein sequence." ;
  rdfs:subClassOf obo:ECO_0007672 .

obo:ECO_0007481 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence based on orthology evidence used in automatic assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence based on orthology evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005545 , obo:ECO_0007482 .

obo:ECO_0006388 a rdfs:Class ;
  rdfs:label "iodine-125-labeled ligand binding evidence used in manual assertion" ;
  rdfs:comment "A type of iodine-125-labeled ligand binding evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006349 , obo:ECO_0007009 .

obo:ECO_0007315 a rdfs:Class ;
  rdfs:label "RNA protection assay evidence used in automatic assertion" ;
  rdfs:comment "A type of RNA protection assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000110 , obo:ECO_0007316 .

obo:GO_0032076 a rdfs:Class ;
  rdfs:label "negative regulation of deoxyribonuclease activity" ;
  rdfs:subClassOf obo:GO_0032070 , obo:GO_0032074 , obo:GO_0051053 , obo:GO_0065007 .

obo:ECO_0001550 a rdfs:Class ;
  rdfs:label "demethylation assay evidence" ;
  rdfs:comment "A type of direct assay evidence where the removal of methyl groups from a substrate (RNA/DNA or protein) is detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007449 a rdfs:Class ;
  rdfs:label "propidium iodide staining evidence used in automatic assertion" ;
  rdfs:comment "A type of propidium iodide staining evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001114 , obo:ECO_0007798 .

obo:ECO_0007776 a rdfs:Class ;
  rdfs:label "DNA synthesis cell proliferation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of DNA synthesis cell proliferation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005008 , obo:ECO_0007775 .

obo:ECO_0005560 a rdfs:Class ;
  rdfs:label "pairwise sequence alignment evidence used in manual assertion" ;
  rdfs:comment "A type of pairwise sequence alignment evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000247 , obo:ECO_0005554 .

obo:GO_0032760 a rdfs:Class ;
  rdfs:label "positive regulation of tumor necrosis factor production" ;
  rdfs:subClassOf obo:GO_0032680 , obo:GO_0065007 , obo:GO_1903557 .

obo:GO_0019222 a rdfs:Class ;
  rdfs:label "regulation of metabolic process" ;
  rdfs:subClassOf obo:GO_0050789 , obo:GO_0065007 .

obo:ECO_0007201 a rdfs:Class ;
  rdfs:label "developmental similarity evidence used in automatic assertion" ;
  rdfs:comment "A type of developmental similarity evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000067 , obo:ECO_0007824 .

obo:ECO_0007109 a rdfs:Class ;
  rdfs:label "beta-galactosidase fusion protein localization evidence used in manual assertion" ;
  rdfs:comment "A type of beta-galactosidase fusion protein localization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000132 , obo:ECO_0000314 .

obo:ECO_0007094 a rdfs:Class ;
  rdfs:label "positional similarity evidence used in manual assertion" ;
  rdfs:comment "A type of positional similarity evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000060 , obo:ECO_0000252 .

obo:ECO_0006217 a rdfs:Class ;
  rdfs:label "author inference used in automatic assertion" ;
  rdfs:comment "A type of author inference that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006185 , obo:ECO_0007319 , obo:ECO_0007832 .

obo:ECO_0006206 a rdfs:Class ;
  rdfs:label "near-UV circular dichroism evidence used in manual assertion" ;
  rdfs:comment "A type of near-UV circular dichroism evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006180 , obo:ECO_0006200 .

obo:GO_0032060 a rdfs:Class ;
  rdfs:label "bleb assembly" ;
  rdfs:subClassOf obo:GO_0120031 .

obo:ECO_0006014 a rdfs:Class ;
  rdfs:label "whole-cell patch-clamp recording evidence" ;
  rdfs:comment "A type of patch-clamp recording evidence in which a glass micropipette filled with a prepared solution is used to form a seal with the cell membrane followed by rupture of membrane to provide accurate and high resolution electrical property measurements of the whole cell." ;
  rdfs:subClassOf obo:ECO_0006012 .

obo:ECO_0007650 a rdfs:Class ;
  rdfs:label "manually integrated combinatorial computational evidence used in automatic assertion" ;
  rdfs:comment "A type of manually integrated combinatorial computational evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007648 , obo:ECO_0007676 , obo:ECO_0007679 .

obo:OBI_0000219 a rdfs:Class ;
  rdfs:label "cellular feature identification objective"@en ;
  rdfs:comment "Cellular_feature_identification_objective is a biological_feature_identification_objective role describing a study designed to examine or characterize a biological feature monitored at the cellular level, e.g. stage of cell cycle, stage of differentiation."@en ;
  rdfs:subClassOf obo:OBI_0000015 .

obo:ECO_0007246 a rdfs:Class ;
  rdfs:label "green fluorescent protein immunolocalization evidence used in automatic assertion" ;
  rdfs:comment "A type of green fluorescent protein immunolocalization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000300 , obo:ECO_0007486 .

obo:ECO_0006308 a rdfs:Class ;
  rdfs:label "turbidity measurement evidence used in automatic assertion" ;
  rdfs:comment "A type of turbidity measurement evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006263 , obo:ECO_0007307 .

obo:ECO_0000057 a rdfs:Class ;
  rdfs:label "phenotypic similarity evidence" ;
  rdfs:comment "A type of similarity based on the expression of a genotype in an environment." ;
  rdfs:subClassOf obo:ECO_0000041 .

obo:OBI_0000437 a rdfs:Class ;
  rdfs:label "analyte measurement objective"@en ;
  rdfs:comment "an assay objective to determine the presence or concentration of an analyte in the evaluant"@en ;
  rdfs:subClassOf obo:OBI_0000441 .

obo:ECO_0005588 a rdfs:Class ;
  rdfs:label "wide-field microscopy evidence" ;
  rdfs:comment "A type of microscopy evidence where imaging of a thin cell or a tissue specimen is performed by full aperture emission of light gathered by the microscope's objective, which maximizes the recorded signal and simultaneously minimizes the required exposure times." ;
  rdfs:subClassOf obo:ECO_0001098 .

obo:ECO_0006254 a rdfs:Class ;
  rdfs:label "site-directed spin-labelling electron paramagnetic resonance evidence" ;
  rdfs:comment "A type of electron paramagnetic resonance evidence where the signal is obtained by labeling the protein in specific sites with a functional group (most often a nitroxide) containing an unpaired electron using site-directed mutagenesis." ;
  rdfs:subClassOf obo:ECO_0006253 .

obo:GO_1903508 a rdfs:Class ;
  rdfs:label "positive regulation of nucleic acid-templated transcription" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_1902680 , obo:GO_1903506 .

obo:OBI_0002059 a rdfs:Class ;
  rdfs:label "assay measuring a binding constant of a T cell epitope:MHC:TCR complex" ;
  rdfs:comment "A T cell epitope recognition assay that quantitavely characterizes the binding of a TCR with a ligand by determining a binding constant. " ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_0003147 .

obo:OBI_0000250 a rdfs:Class ;
  rdfs:label "dye role"@en ;
  rdfs:comment "A molecular label role which inheres in a material entity and which is realized in the process of detecting a molecular dye that imparts color to some material of interest. " ;
  rdfs:subClassOf obo:OBI_0002743 .

obo:ECO_0006025 a rdfs:Class ;
  rdfs:label "air-liquid interface assay evidence used in manual assertion" ;
  rdfs:comment "A type of air-liquid interface assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006021 , obo:ECO_0006024 .

obo:ECO_0008022 a rdfs:Class ;
  rdfs:label "regular expression method evidence used in manual assertion" ;
  rdfs:comment "A type of regular expression method evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008012 , obo:ECO_0008020 .

obo:ECO_0001134 a rdfs:Class ;
  rdfs:label "urine test evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from the analysis of a urine sample." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:OBI_0001669 a rdfs:Class ;
  rdfs:label "far-western blot assay" ;
  rdfs:comment "An assay that measures protein-protein interactions by separating target proteins on an SDS-PAGE gel, transfering them to a solid substrate, hybridizing with a protein probe and visualizing bound proteins using a probe-directed antibody. This is a adaptation on the western blot assay." ;
  rdfs:subClassOf obo:OBI_0001146 .

obo:OBI_0000397 a rdfs:Class ;
  rdfs:label "image acquisition function"@en ;
  rdfs:comment "An image acquisition function is a function to acquire an image of a material"@en ;
  rdfs:subClassOf obo:OBI_0000453 .

obo:ECO_0008153 a rdfs:Class ;
  rdfs:label "antibody SF1-1.1.1-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody SF1-1.1.1-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008072 , obo:ECO_0008087 .

obo:ECO_0007834 a rdfs:Class ;
  rdfs:label "curator inference from encyclopedia used in automatic assertion" ;
  rdfs:comment "A type of curator inference from encyclopedia that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007637 , obo:ECO_0007833 .

obo:GO_0030334 a rdfs:Class ;
  rdfs:label "regulation of cell migration" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_2000145 .

obo:OBI_0001444 a rdfs:Class ;
  rdfs:label "genotyping design" ;
  rdfs:comment "A study design that classifies an individual or group of individuals on the basis of alleles, haplotypes, SNPs." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:ECO_0006078 a rdfs:Class ;
  rdfs:label "immunological assay evidence used in manual assertion" ;
  rdfs:comment "A type of affinity evidence that is used in manual assertion resulting from quantitation of the analyte which depends on the reaction of an antigen (analyte) and an antibody." ;
  rdfs:subClassOf obo:ECO_0000040 , obo:ECO_0006075 .

obo:ECO_0000051 a rdfs:Class ;
  rdfs:label "genetic similarity evidence" ;
  rdfs:comment "A type of similarity based on genotype without respect to expression." ;
  rdfs:subClassOf obo:ECO_0000041 .

obo:ECO_0007409 a rdfs:Class ;
  rdfs:label "in vitro ubiquitination assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro ubiquitination assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001073 , obo:ECO_0007801 .

obo:ECO_0001238 a rdfs:Class ;
  rdfs:label "nuclear magnetic resonance evidence used in manual assertion" ;
  rdfs:comment "A type of nuclear magnetic resonance evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001105 , obo:ECO_0006285 .

obo:ECO_0007598 a rdfs:Class ;
  rdfs:label "direct Coombs test evidence used in automatic assertion" ;
  rdfs:comment "A type of direct Coombs test evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007023 , obo:ECO_0007596 .

obo:ECO_0000068 a rdfs:Class ;
  rdfs:label "yeast 2-hybrid evidence" ;
  rdfs:comment "A type of bait-prey hybrid interaction evidence that is based on detection of protein-protein interaction by activation of a yeast reporter gene after a bait protein fused to a DNA-binding domain (which has been transfected into a yeast cell) is used to screen a cDNA library of clones fused to an activation domain." ;
  rdfs:subClassOf obo:ECO_0000025 .

obo:GO_0090304 a rdfs:Class ;
  rdfs:label "nucleic acid metabolic process" ;
  rdfs:subClassOf obo:GO_0006139 , obo:GO_0043170 .

obo:ECO_0005802 a rdfs:Class ;
  rdfs:label "cell transfection experiment evidence used in manual assertion"@en ;
  rdfs:comment "A type of cell transfection experiment evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0005606 .

obo:ECO_0007116 a rdfs:Class ;
  rdfs:label "biochemical trait analysis evidence used in manual assertion" ;
  rdfs:comment "A type of biochemical trait analysis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000172 , obo:ECO_0007634 .

obo:ECO_0008044 a rdfs:Class ;
  rdfs:label "antibody 34-5-8S-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody 34-5-8S to determine that the H2-Dd protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0007281 a rdfs:Class ;
  rdfs:label "cell-detached inside-out single-channel recording evidence used in automatic assertion" ;
  rdfs:comment "A type of cell-detached inside-out single-channel recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005574 , obo:ECO_0007537 .

obo:OBI_1000029 a rdfs:Class ;
  rdfs:label "phage display library"@en ;
  rdfs:comment "a phage display library is a collection of materials in which a mixture of genes or gene fragments is expressed and can be individually selected and amplified."@en ;
  rdfs:subClassOf obo:OBI_0000731 .

obo:ECO_0007550 a rdfs:Class ;
  rdfs:label "genetically encoded fluorescent electrophysiology assay evidence used in automatic assertion" ;
  rdfs:comment "A type of genetically encoded fluorescent electrophysiology assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006038 , obo:ECO_0007535 , obo:ECO_0007548 .

obo:GO_0001818 a rdfs:Class ;
  rdfs:label "negative regulation of cytokine production" ;
  rdfs:subClassOf obo:GO_0001817 , obo:GO_0010629 , obo:GO_0051241 , obo:GO_0065007 .

obo:ECO_0007329 a rdfs:Class ;
  rdfs:label "biological aspect of descendant evidence used in automatic assertion" ;
  rdfs:comment "A type of biological aspect of descendant evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000214 , obo:ECO_0007826 .

obo:OBI_0000192 a rdfs:Class ;
  rdfs:label "microtiter plate" ;
  rdfs:comment "A microtiter_plate is a flat plate with multiple wells used as small test tubes." ;
  rdfs:subClassOf obo:OBI_0000967 .

obo:ECO_0000221 a rdfs:Class ;
  rdfs:label "high throughput nucleotide sequencing assay evidence" ;
  rdfs:comment "A type of nucleotide sequencing assay evidence in which a sequence is derived from a parallelized sequencing technique." ;
  rdfs:subClassOf obo:BFO_0000002 , obo:ECO_0000219 .

obo:OBI_0000690 a rdfs:Class ;
  rdfs:label "immunoprecipitation" ;
  rdfs:comment "is a process which realizes a material separation objective by relying on antibodies to specifically binding to material entity" ;
  rdfs:subClassOf obo:OBI_0600014 .

obo:OBI_0600007 a rdfs:Class ;
  rdfs:label "administering substance in vivo"@en ;
  rdfs:comment "A process by which a substance is intentionally given to an organism resulting in exposure of the organism to that substance." ;
  rdfs:subClassOf obo:OBI_0000274 .

obo:ECO_0008099 a rdfs:Class ;
  rdfs:label "antibody 34-7-23S-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody 34-7-23S-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008045 , obo:ECO_0008087 .

obo:IAO_0000015 a rdfs:Class ;
  rdfs:label "information carrier"@en ;
  rdfs:comment "A quality of an information bearer that imparts the information content"@en ;
  rdfs:subClassOf obo:BFO_0000019 .

obo:ECO_0000040 a rdfs:Class ;
  rdfs:label "immunological assay evidence" ;
  rdfs:comment "A type of affinity evidence resulting from quantitation of the analyte which depends on the reaction of an antigen (analyte) and an antibody." ;
  rdfs:subClassOf obo:ECO_0000023 .

obo:OBI_0000439 a rdfs:Class ;
  rdfs:label "agarose gel"@en ;
  rdfs:comment "a material entity resulting from the polymerization of agarose after heating agarose suspended in some buffer solution"@en ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:OBI_0400149 a rdfs:Class ;
  rdfs:label "protein microarray" ;
  rdfs:comment "A protein-microarray is a microarray, ususlly a piece of glass, on which different molecules of protein have been affixed at separate locations in an ordered manner. These are used to identify protein-protein or protein-small molecule interactions." ;
  rdfs:subClassOf obo:OBI_0400147 .

obo:GO_0032303 a rdfs:Class ;
  rdfs:label "regulation of icosanoid secretion" ;
  rdfs:subClassOf obo:GO_0044070 , obo:GO_0051046 , obo:GO_0065007 , obo:GO_2000191 .

obo:ECO_0007037 a rdfs:Class ;
  rdfs:label "5-cyano-2,3-ditolyl tetrazolium chloride staining evidence" ;
  rdfs:comment "A type of staining evidence that involves the use of the redox dye 5-cyano-2,3-ditolyl tetrazolium chloride to evaluate the respiratory activity of cells." ;
  rdfs:subClassOf obo:ECO_0005019 .

obo:ECO_0001253 a rdfs:Class ;
  rdfs:label "quantitative mass spectrometry evidence used in manual assertion" ;
  rdfs:comment "A type of quantitative mass spectrometry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001119 , obo:ECO_0001230 .

obo:CL_0000578 a rdfs:Class ;
  rdfs:label "experimentally modified cell in vitro" ;
  rdfs:comment "A cell in vitro that has undergone physical changes as a consequence of a deliberate and specific experimental procedure." ;
  rdfs:subClassOf obo:CL_0000000 , obo:OBI_0000047 .

obo:ECO_0007707 a rdfs:Class ;
  rdfs:label "protein inhibition evidence used in manual assertion" ;
  rdfs:comment "A type of protein inhibition evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000020 , obo:ECO_0000314 .

obo:ECO_0001069 a rdfs:Class ;
  rdfs:label "in vitro polyADP-ribosylation assay evidence" ;
  rdfs:comment "A type of polyADP-ribosylation assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001557 .

obo:ECO_0007148 a rdfs:Class ;
  rdfs:label "pulsed-field gel electrophoresis evidence used in manual assertion" ;
  rdfs:comment "A type of pulsed-field gel electrophoresis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0000338 .

obo:ECO_0000071 a rdfs:Class ;
  rdfs:label "morphological similarity evidence" ;
  rdfs:comment "A type of phenotypic similarity evidence based on the similarity of shape, structure, or configuration in structures." ;
  rdfs:subClassOf obo:ECO_0000057 .

obo:OBI_0000376 a rdfs:Class ;
  rdfs:label "synthesizing function"@en ;
  rdfs:comment "A synthesizing function is a function to assemble new output materials from distinct input materials.  The output materials typically consist of chemically distinct monomeric objects or object aggregate polymers." ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:ECO_0007393 a rdfs:Class ;
  rdfs:label "in vitro acetylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro acetylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001057 , obo:ECO_0007794 .

obo:ECO_0006180 a rdfs:Class ;
  rdfs:label "near-UV circular dichroism evidence" ;
  rdfs:comment "A type of circular dichroism evidence whose spectrum is in the range of 250-350 nm , providing information mainly about aromatic residues, i.e. phenylalanine, tyrosine and tryptophan, through which it provides information on the tertiary structure of a protein." ;
  rdfs:subClassOf obo:ECO_0006177 .

obo:ECO_0000033 a rdfs:Class ;
  rdfs:label "author statement supported by traceable reference" ;
  rdfs:comment "A type of author statement in which the author makes a statement that is not supported by information in that particular publication, but rather can be traced to a reference cited by that publication." ;
  rdfs:subClassOf obo:ECO_0000204 .

obo:ECO_0005564 a rdfs:Class ;
  rdfs:label "current density evidence" ;
  rdfs:comment "A type of electrophysiology assay evidence that involves measuring the amount of current (pA) going through the ion channels per unit  of membrane." ;
  rdfs:subClassOf obo:ECO_0000164 .

obo:ECO_0007733 a rdfs:Class ;
  rdfs:label "nucleotide sequencing assay evidence used in manual assertion" ;
  rdfs:comment "A type of nucleotide sequencing assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000219 , obo:ECO_0000269 .

obo:ECO_0001112 a rdfs:Class ;
  rdfs:label "peptide array evidence" ;
  rdfs:comment "A type of protein binding evidence resulting from protein-bound peptides detected from a collection of peptides arranged as an array and incubated with the partner protein in order to map protein-protein interaction sites." ;
  rdfs:subClassOf obo:ECO_0000024 .

obo:ECO_0008066 a rdfs:Class ;
  rdfs:label "antibody M1/42-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody M1/42 to determine that the mouse MHC class I protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0006276 a rdfs:Class ;
  rdfs:label "analytical ultracentrifugation evidence used in automatic assertion" ;
  rdfs:comment "A type of analytical ultracentrifugation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006247 , obo:ECO_0007816 .

obo:GO_0009653 a rdfs:Class ;
  rdfs:label "anatomical structure morphogenesis" ;
  rdfs:subClassOf obo:GO_0032502 .

obo:ECO_0007787 a rdfs:Class ;
  rdfs:label "protein separation evidence used in automatic assertion" ;
  rdfs:comment "A type of protein separation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000156 , obo:ECO_0007534 .

obo:OBI_0000809 a rdfs:Class ;
  rdfs:label "amplified DNA" ;
  rdfs:comment "DNA that has been produced in an enzymatic amplification process" ;
  rdfs:subClassOf obo:CHEBI_16991 , obo:OBI_0000047 .

obo:ECO_0006228 a rdfs:Class ;
  rdfs:label "Fourier-transform infrared spectroscopy evidence used in manual assertion" ;
  rdfs:comment "A type of Fourier-transform infrared spectroscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001258 , obo:ECO_0006191 .

obo:OBI_0000441 a rdfs:Class ;
  rdfs:label "assay objective"@en ;
  rdfs:comment "an objective specification to determine a specified type of information about an evaluated entity (the material entity bearing evaluant role)"@en ;
  rdfs:subClassOf obo:IAO_0000005 .

obo:ECO_0007014 a rdfs:Class ;
  rdfs:label "combinatorial experimental and curator inference evidence used in manual assertion" ;
  rdfs:comment "A type of combinatorial evidence from curator knowledge and experimental evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000244 , obo:ECO_0007012 .

obo:OBI_0001057 a rdfs:Class ;
  rdfs:label "high performance liquid chromatography instrument" ;
  rdfs:comment "A liquid chromatography instrument that consists of a reservoir of mobile phase, a pump, an injector, a separation column, and a detector. The pump (rather than gravity) provides the higher pressure required to propel the mobile phase and analyte through the densely packed column." ;
  rdfs:subClassOf obo:OBI_0001139 .

obo:ECO_0007112 a rdfs:Class ;
  rdfs:label "protein separation followed by direct sequencing evidence used in manual assertion" ;
  rdfs:comment "A type of protein separation followed by direct sequencing evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000158 , obo:ECO_0006005 .

obo:GO_0032305 a rdfs:Class ;
  rdfs:label "positive regulation of icosanoid secretion" ;
  rdfs:subClassOf obo:GO_0032303 , obo:GO_0051047 , obo:GO_0065007 , obo:GO_1903793 , obo:GO_2000193 .

obo:ECO_0007819 a rdfs:Class ;
  rdfs:label "protein detection assay evidence used in automatic assertion" ;
  rdfs:comment "A type of protein detection assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005517 , obo:ECO_0007816 .

obo:ECO_0000350 a rdfs:Class ;
  rdfs:label "partial support of intron positions by RNA-sequencing alignment evidence used in automatic assertion" ;
  rdfs:comment "A type of partial support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000344 , obo:ECO_0000347 .

obo:ECO_0007335 a rdfs:Class ;
  rdfs:label "3D cell culture evidence used in automatic assertion" ;
  rdfs:comment "A type of 3D cell culture evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001001 , obo:ECO_0007775 .

obo:ECO_0007464 a rdfs:Class ;
  rdfs:label "tryptic phosphopeptide mapping assay evidence used in automatic assertion" ;
  rdfs:comment "A type of tryptic phosphopeptide mapping assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001132 , obo:ECO_0007433 .

obo:GO_0060195 a rdfs:Class ;
  rdfs:label "negative regulation of antisense RNA transcription" ;
  rdfs:subClassOf obo:GO_0045892 , obo:GO_0060194 , obo:GO_0065007 .

obo:ECO_0008077 a rdfs:Class ;
  rdfs:label "antibody Y3JP-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody Y3JP to determine that the mouse MHC class II protein complex and/or rat MHC class II protein complex with RT1-A haplotype presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:GO_0071705 a rdfs:Class ;
  rdfs:label "nitrogen compound transport" ;
  rdfs:subClassOf obo:GO_0006810 .

obo:ECO_0008145 a rdfs:Class ;
  rdfs:label "antibody ME1-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody ME1-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008068 , obo:ECO_0008087 .

obo:ECO_0001126 a rdfs:Class ;
  rdfs:label "spectrophotometry evidence" ;
  rdfs:comment "A type of spectrometry evidence resulting from the evaluation of a molecule in a fluid or solid by its ability to alter the transmission of light at specific wavelengths." ;
  rdfs:subClassOf obo:BFO_0000002 , obo:ECO_0005504 .

obo:ECO_0000025 a rdfs:Class ;
  rdfs:label "bait-prey hybrid interaction evidence" ;
  rdfs:comment "A type of protein binding evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." ;
  rdfs:subClassOf obo:ECO_0006351 .

obo:ECO_0007267 a rdfs:Class ;
  rdfs:label "insertion mutation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of insertion mutation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005509 , obo:ECO_0007325 .

obo:ECO_0007066 a rdfs:Class ;
  rdfs:label "static light scattering assay evidence used in manual assertion" ;
  rdfs:comment "A type of static light scattering assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007062 , obo:ECO_0007065 .

obo:GO_0044093 a rdfs:Class ;
  rdfs:label "positive regulation of molecular function" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_0065009 .

obo:ECO_0001101 a rdfs:Class ;
  rdfs:label "MTT assay evidence" ;
  rdfs:comment "A type of apoptotic assay evidence resulting from the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to its insoluble purple formazan dye in viable cells and measures cell viability / metabolic activity." ;
  rdfs:subClassOf obo:ECO_0005034 .

obo:GO_1901701 a rdfs:Class ;
  rdfs:label "cellular response to oxygen-containing compound" ;
  rdfs:subClassOf obo:GO_0070887 , obo:GO_1901700 .

obo:ECO_0001804 a rdfs:Class ;
  rdfs:label "beta-glucuronidase reporter gene assay evidence" ;
  rdfs:comment "A type of reporter gene assay evidence where the beta-glucuronidase enzyme from Escherichia coli is used as the reporter to transform non-fluorescent substrates into fluorescents for detection." ;
  rdfs:subClassOf obo:ECO_0000049 .

obo:ECO_0005640 a rdfs:Class ;
  rdfs:label "glutathione S-transferase pull-down assay evidence used in manual assertion" ;
  rdfs:comment "A type of glutathione S-transferase pull-down assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001817 , obo:ECO_0006077 .

obo:ECO_0008160 a rdfs:Class ;
  rdfs:label "antibody W6/32-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody W6/32-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008075 , obo:ECO_0008088 .

obo:ECO_0001185 a rdfs:Class ;
  rdfs:label "histochemistry evidence used in manual assertion" ;
  rdfs:comment "A type of histochemistry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001050 , obo:ECO_0001187 .

obo:GO_0032368 a rdfs:Class ;
  rdfs:label "regulation of lipid transport" ;
  rdfs:subClassOf obo:GO_0051049 , obo:GO_0065007 , obo:GO_1905952 .

obo:ECO_0008186 a rdfs:Class ;
  rdfs:label "avidity-based extracellular interaction screening evidence" ;
  rdfs:comment "A type of avidity evidence in which immobilized \"bait\" proteins are probed with enzyme-tagged, multimerized \"prey\" proteins to amplify avidity, enabling sensitive detection of otherwise weak and transient extracellular protein-protein interactions." ;
  rdfs:subClassOf obo:ECO_0008185 .

obo:PR_000000001 a rdfs:Class ;
  rdfs:label "protein" ;
  rdfs:comment "An amino acid chain that is produced de novo by ribosome-mediated translation of a genetically-encoded mRNA, and any derivatives thereof." ;
  rdfs:subClassOf obo:CHEBI_16670 .

obo:GO_0050793 a rdfs:Class ;
  rdfs:label "regulation of developmental process" ;
  rdfs:subClassOf obo:GO_0050789 , obo:GO_0065007 .

obo:ECO_0006047 a rdfs:Class ;
  rdfs:label "field potential recording evidence" ;
  rdfs:comment "A type of extracellular recording evidence in which electrical activity is measured in either tissue or at a cellular level with microelectrodes." ;
  rdfs:subClassOf obo:ECO_0006043 .

obo:ECO_0007077 a rdfs:Class ;
  rdfs:label "colony morphology phenotypic evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence resulting from studying the physical characteristics of an assemblage of microorganisms growing on a solid surface such as the surface of an agar culture medium." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:OBI_0000274 a rdfs:Class ;
  rdfs:label "adding a material entity into a target"@en ;
  rdfs:comment "is a process with the objective to place a material entity bearing the 'material to be added role' into a material bearing the 'target of material addition role'."@en ;
  rdfs:subClassOf obo:OBI_0000652 .

obo:GO_0044087 a rdfs:Class ;
  rdfs:label "regulation of cellular component biogenesis" ;
  rdfs:subClassOf obo:GO_0050794 , obo:GO_0065007 .

obo:OBI_0000671 a rdfs:Class ;
  rdfs:label "sample from organism" ;
  rdfs:comment "a material obtained from an organism in order to be a representative of the whole" ;
  rdfs:subClassOf obo:OBI_0000747 , obo:OBI_0001479 .

obo:GO_0033036 a rdfs:Class ;
  rdfs:label "macromolecule localization" ;
  rdfs:comment "Any process in which a macromolecule is transported to, or maintained in, a specific location." ;
  rdfs:subClassOf obo:GO_0051179 .

obo:ECO_0008171 a rdfs:Class ;
  rdfs:label "MHC complex binding prediction based MHC restriction evidence used in manual assertion" ;
  rdfs:comment "A type of MHC complex binding prediction based MHC restriction evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007668 , obo:ECO_0008081 .

obo:IAO_0000101 a rdfs:Class ;
  rdfs:label "image"@en ;
  rdfs:comment "An image is an affine projection to a two dimensional surface, of measurements of some quality of an entity or entities repeated at regular intervals across a spatial range, where the measurements are represented as color and luminosity on the projected on surface."@en ;
  rdfs:subClassOf obo:IAO_0000308 .

obo:ECO_0005608 a rdfs:Class ;
  rdfs:label "tumble frequency assay evidence" ;
  rdfs:comment "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined by measuring chemotaxis in response to sudden concentration changes from one uniform spatial environment to another." ;
  rdfs:subClassOf obo:ECO_0005021 .

obo:GO_0031345 a rdfs:Class ;
  rdfs:label "negative regulation of cell projection organization" ;
  rdfs:subClassOf obo:GO_0031344 , obo:GO_0051129 , obo:GO_0065007 .

obo:ECO_0006056 a rdfs:Class ;
  rdfs:label "high throughput evidence used in manual assertion" ;
  rdfs:comment "A type of evidence that is used in a manual assertion where data generation is automated with equipment to allow for assaying large numbers of samples or molecules in parallel." ;
  rdfs:subClassOf obo:ECO_0000352 , obo:ECO_0006055 .

obo:ECO_0000282 a rdfs:Class ;
  rdfs:label "heterologous protein expression evidence used in manual assertion" ;
  rdfs:comment "A type of heterologous protein expression analysis that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000154 , obo:ECO_0000284 .

obo:ECO_0000200 a rdfs:Class ;
  rdfs:label "sequence alignment evidence" ;
  rdfs:comment "A type of sequence similarity evidence in which an annotation is made based on a manually-reviewed or published sequence alignment" ;
  rdfs:subClassOf obo:ECO_0000044 .

obo:ECO_0007744 a rdfs:Class ;
  rdfs:label "combinatorial computational and experimental evidence used in manual assertion" ;
  rdfs:comment "A type of combinatorial computational and experimental evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000244 , obo:ECO_0007661 .

obo:ECO_0007824 a rdfs:Class ;
  rdfs:label "phenotypic similarity evidence used in automatic assertion" ;
  rdfs:comment "A type of phenotypic similarity evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000057 , obo:ECO_0000251 .

obo:ECO_0008052 a rdfs:Class ;
  rdfs:label "antibody B8-24-3-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody B8-24-3 to determine that the mouse MHC class I protein complex with H2-b haplotype and/or mouse MHC protein complex with H2-p haplotype presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0007239 a rdfs:Class ;
  rdfs:label "3C-seq evidence used in automatic assertion" ;
  rdfs:comment "A type of 3C-seq evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000239 , obo:ECO_0007822 .

obo:ECO_0007296 a rdfs:Class ;
  rdfs:label "motility assay evidence used in automatic assertion" ;
  rdfs:comment "A type of motility assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005803 , obo:ECO_0007307 .

obo:ECO_0006366 a rdfs:Class ;
  rdfs:label "temperature jump relaxation evidence used in manual assertion" ;
  rdfs:comment "A type of temperature jump relaxation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001249 , obo:ECO_0006338 .

obo:OBI_0000185 a rdfs:Class ;
  rdfs:label "imaging assay" ;
  rdfs:comment "An assay that produces a picture of an entity." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:GO_0032091 a rdfs:Class ;
  rdfs:label "negative regulation of protein binding" ;
  rdfs:subClassOf obo:GO_0043393 , obo:GO_0051100 , obo:GO_0065007 .

obo:OBI_0003127 a rdfs:Class ;
  rdfs:label "B cell epitope assay" ;
  rdfs:comment "An immune epitope assay that characterizes the structrue of an antibody-epitope complex, or measures the binding of an antibody receptor to epitope, or the immune response process resulting from such a binding event or the recognition of the epitope." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_1110128 .

obo:ECO_0007658 a rdfs:Class ;
  rdfs:label "automatically integrated combinatorial experimental evidence" ;
  rdfs:comment "A type of automatically integrated combinatorial evidence in which two or more distinct types of experimental evidence are integrated automatically." ;
  rdfs:subClassOf obo:ECO_0007654 , obo:ECO_0007673 .

obo:ECO_0007352 a rdfs:Class ;
  rdfs:label "Boyden chamber assay evidence used in automatic assertion" ;
  rdfs:comment "A type of Boyden chamber assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001017 , obo:ECO_0007780 .

obo:GO_0008285 a rdfs:Class ;
  rdfs:label "negative regulation of cell population proliferation" ;
  rdfs:subClassOf obo:GO_0042127 , obo:GO_0048523 , obo:GO_0065007 .

obo:OBI_0002020 a rdfs:Class ;
  rdfs:label "epigenetic modification assay" ;
  rdfs:comment "An assay that identifies epigenetic modifications including histone modifications, open chromatin, and DNA methylation." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0000340 a rdfs:Class ;
  rdfs:label "plasmid maintenance assay evidence" ;
  rdfs:comment "A type of experimental evidence in which a plasmid that is capable of being replicated is introduced into cells, cells are grown without selection for several generations, and retention of the plasmid is determined." ;
  rdfs:subClassOf obo:ECO_0000006 , obo:IAO_0000030 .

obo:GO_0006915 a rdfs:Class ;
  rdfs:label "apoptotic process" ;
  rdfs:subClassOf obo:GO_0012501 .

obo:ECO_0007565 a rdfs:Class ;
  rdfs:label "immuno-labelling electron microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of immuno-labelling electron microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006070 , obo:ECO_0007493 .

obo:CHEBI_16670 a rdfs:Class ;
  rdfs:label "peptide" ;
  rdfs:comment "Amide derived from two or more amino carboxylic acid molecules (the same or different) by formation of a covalent bond from the carbonyl carbon of one to the nitrogen atom of another with formal loss of water. The term is usually applied to structures formed from alpha-amino acids, but it includes those derived from any amino carboxylic acid. X = OH, OR, NH2, NHR, etc." ;
  rdfs:subClassOf obo:CHEBI_33839 .

obo:OBI_0001138 a rdfs:Class ;
  rdfs:label "X-ray source" ;
  rdfs:comment "A device that is used to generate X-rays." ;
  rdfs:subClassOf obo:OBI_0000968 .

obo:ECO_0007453 a rdfs:Class ;
  rdfs:label "protein sequencing assay evidence used in automatic assertion" ;
  rdfs:comment "A type of protein sequencing assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001118 , obo:ECO_0007812 .

obo:OBI_0001361 a rdfs:Class ;
  rdfs:label "transcription profiling by RT-PCR assay" ;
  rdfs:comment "A transcription profiling assay that uses reverse transcription PCR (RT-PCR)." ;
  rdfs:subClassOf obo:OBI_0000424 , obo:OBI_0002692 .

obo:ECO_0000020 a rdfs:Class ;
  rdfs:label "protein inhibition evidence" ;
  rdfs:comment "A type of direct assay evidence based on the inhibition of the molecular function of a protein." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007813 a rdfs:Class ;
  rdfs:label "nucleotide sequencing assay evidence used in automatic assertion" ;
  rdfs:comment "A type of nucleotide sequencing assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000219 , obo:ECO_0007812 .

obo:GO_0090342 a rdfs:Class ;
  rdfs:label "regulation of cell aging" ;
  rdfs:subClassOf obo:GO_0050793 , obo:GO_0050794 , obo:GO_0065007 .

obo:OBI_0302733 a rdfs:Class ;
  rdfs:label "solute role"@en ;
  rdfs:comment "solute role is a role played by a chemical entity which is dissolved by another chemical entity (the solvent) when creating a solution"@en ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:ECO_0001843 a rdfs:Class ;
  rdfs:label "random mutagenesis of specific target DNA evidence used in automatic assertion" ;
  rdfs:comment "A type of random mutagenesis of specific target DNA evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001842 , obo:ECO_0007478 .

obo:ECO_0000363 a rdfs:Class ;
  rdfs:label "computational inference used in automatic assertion" ;
  rdfs:comment "A type of evidence based on computational logical inference that is used in automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000362 , obo:ECO_0007669 , obo:ECO_0007832 .

obo:ECO_0005656 a rdfs:Class ;
  rdfs:label "methylation interference footprinting evidence used in manual assertion" ;
  rdfs:comment "A type of methylation interference footprinting evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001827 , obo:ECO_0006083 .

obo:ECO_0007529 a rdfs:Class ;
  rdfs:label "ad-hoc quantitative phenotype observation evidence used in automatic assertion" ;
  rdfs:comment "A type of ad-hoc quantitative phenotype observation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005675 , obo:ECO_0007635 .

obo:ECO_0006313 a rdfs:Class ;
  rdfs:label "urea-induced protein unfolding evidence used in manual assertion" ;
  rdfs:comment "A type of urea-induced protein unfolding evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006266 , obo:ECO_0006311 .

obo:GO_0071310 a rdfs:Class ;
  rdfs:label "cellular response to organic substance" ;
  rdfs:subClassOf obo:GO_0010033 , obo:GO_0070887 .

obo:ECO_0005553 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence by experimental evidence from single species" ;
  rdfs:comment "A type of biological system reconstruction where the experimental evidence is derived by using multiple experiments in a single species." ;
  rdfs:subClassOf obo:ECO_0005551 .

obo:ECO_0005537 a rdfs:Class ;
  rdfs:label "xylE reporter gene assay evidence" ;
  rdfs:comment "A type of reporter gene assay evidence based on the fusion of the xylE gene to a specific promoter for the expression of catechol 2,3-dioxygenase that converts the colorless catechol substrate to yellow 2-hydroxymuconic semialdehyde for identification of the expression of a particular gene." ;
  rdfs:subClassOf obo:ECO_0000049 .

obo:OBI_0400065 a rdfs:Class ;
  rdfs:label "light source" ;
  rdfs:comment "A light source is an optical subsystem that provides light for use in a distant area using a delivery system (e.g., fiber optics). Light sources may include one of a variety of lamps (e.g., xenon, halogen, mercury). Most light sources are operated from line power, but some may be powered from batteries. They are mostly used in endoscopic, microscopic, and other examination and/or in surgical procedures. The light source is part of the optical subsystem. In a flow cytometer the light source directs high intensity light at particles at the interrogation point. The light source in a flow cytometer is usually a laser." ;
  rdfs:subClassOf obo:OBI_0001032 .

obo:ECO_0000152 a rdfs:Class ;
  rdfs:label "in vitro recombinant protein transcription reconstitution assay evidence" ;
  rdfs:comment "A type of in vitro transcription reconstitution assay evidence resulting from the use of recombinant proteins in preparation of a fully-defined transcription system." ;
  rdfs:subClassOf obo:ECO_0000150 .

obo:ECO_0008060 a rdfs:Class ;
  rdfs:label "antibody HB180-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody HB180 to determine that the human MHC class II protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0007256 a rdfs:Class ;
  rdfs:label "single exon transcript confirmation via alignment evidence used in automatic assertion" ;
  rdfs:comment "A type of single exon transcript confirmation via alignment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000248 , obo:ECO_0000345 .

obo:ECO_0007055 a rdfs:Class ;
  rdfs:label "differential interference contrast microscopy evidence" ;
  rdfs:comment "A type of microscopy evidence that results in visualization of living cells and transparent specimens by taking advantage of differences in the light refraction of different parts of the specimen." ;
  rdfs:subClassOf obo:ECO_0001098 .

obo:OBI_0001721 a rdfs:Class ;
  rdfs:label "3D molecular structure determination assay of an antigen:antibody complex" ;
  rdfs:comment "A 3D structure determination of bound molecular complex assay that characterizes the 3-dimensional structure of an antigen:antobody complex." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_0001145 , obo:OBI_0003127 .

obo:OBI_0000253 a rdfs:Class ;
  rdfs:label "artificially induced nucleic acid hybridization"@en ;
  rdfs:comment "Is a material transformation in which strands of nucleic acids that are (somewhat) complementary form a double-stranded molecule. Has input at least two single stranded molecules of nucleic acid molecules."@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0007137 a rdfs:Class ;
  rdfs:label "tissue ablation phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of tissue ablation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000242 , obo:ECO_0007634 .

obo:GO_0006448 a rdfs:Class ;
  rdfs:label "regulation of translational elongation" ;
  rdfs:subClassOf obo:GO_0006417 , obo:GO_0065007 .

obo:GO_0032411 a rdfs:Class ;
  rdfs:label "positive regulation of transporter activity" ;
  rdfs:subClassOf obo:GO_0032409 , obo:GO_0044093 , obo:GO_0051050 , obo:GO_0065007 .

obo:ECO_0001231 a rdfs:Class ;
  rdfs:label "medical imaging evidence used in manual assertion" ;
  rdfs:comment "A type of medical imaging evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001097 , obo:ECO_0001256 .

obo:ECO_0001270 a rdfs:Class ;
  rdfs:label "restriction landmark genomic scanning evidence used in manual assertion" ;
  rdfs:comment "A type of restriction landmark genome scanning evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000089 , obo:ECO_0000269 .

obo:ECO_0001084 a rdfs:Class ;
  rdfs:label "in vivo phosphatase assay evidence" ;
  rdfs:comment "A type of phosphatase assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001556 .

obo:GO_0032774 a rdfs:Class ;
  rdfs:label "RNA biosynthetic process" ;
  rdfs:subClassOf obo:GO_0009059 , obo:GO_0016070 , obo:GO_0034654 .

obo:OBI_0003119 a rdfs:Class ;
  rdfs:label "antigen:antibody complex" ;
  rdfs:comment "A complex of molecular entitites which is the result of an antibody binding to an antigen." ;
  rdfs:subClassOf obo:COB_0000080 .

obo:ECO_0007521 a rdfs:Class ;
  rdfs:label "quantitative polymerase chain reaction evidence used in automatic assertion" ;
  rdfs:comment "A type of quantitative polymerase chain reaction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000231 , obo:ECO_0007817 .

obo:GO_1902337 a rdfs:Class ;
  rdfs:label "regulation of apoptotic process involved in morphogenesis" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_1904748 .

obo:GO_1900118 a rdfs:Class ;
  rdfs:label "negative regulation of execution phase of apoptosis" ;
  rdfs:subClassOf obo:GO_0043066 , obo:GO_0065007 , obo:GO_1900117 .

obo:OBI_1110127 a rdfs:Class ;
  rdfs:label "assay measuring binding of a B cell epitope:antibody complex" ;
  rdfs:comment "A B cell epitope assay that measures the binding of an antibody receptor to an epitope." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_0003127 .

obo:ECO_0007793 a rdfs:Class ;
  rdfs:label "ex vivo assay evidence used in automatic assertion" ;
  rdfs:comment "A type of ex vivo assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005502 , obo:ECO_0007307 .

obo:ECO_0007326 a rdfs:Class ;
  rdfs:label "genetic interaction evidence used in automatic assertion" ;
  rdfs:comment "A type of genetic interaction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000011 , obo:ECO_0007635 .

obo:ECO_0006191 a rdfs:Class ;
  rdfs:label "Fourier-transform infrared spectroscopy evidence" ;
  rdfs:comment "A type of spectrophotometry evidence resulting from the evaluation of a molecule in a fluid or solid state by its ability to alter the transmission of infrared light, where the raw data collected is transformed into the specturm by Fourier transform." ;
  rdfs:subClassOf obo:ECO_0001126 .

obo:ECO_0000183 a rdfs:Class ;
  rdfs:label "cell-free assay evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from studies in which cytoplasmic and/or nuclear cellular components from resting, nonstimulated cells, are isolated by ultracentrifugation to provide molecular machinery that can be used in reactions in the absence of many of the other cellular components for the purpose of in vitro protein synthesis, transcription, DNA replication, etc." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:GO_1904748 a rdfs:Class ;
  rdfs:label "regulation of apoptotic process involved in development" ;
  rdfs:subClassOf obo:GO_0042981 , obo:GO_0050793 , obo:GO_0065007 .

obo:BFO_0000019 a rdfs:Class ;
  rdfs:label "quality"@en ;
  rdfs:subClassOf obo:BFO_0000020 .

obo:ECO_0006265 a rdfs:Class ;
  rdfs:label "protein unfolding evidence" ;
  rdfs:comment "A type of structure determination evidence obtained by monitoring structural changes of a protein as a response to changes of an environmental parameter, such as pH or temperature, or the concentration of a denaturant, such as urea." ;
  rdfs:subClassOf obo:ECO_0005031 .

obo:GO_0009889 a rdfs:Class ;
  rdfs:label "regulation of biosynthetic process" ;
  rdfs:subClassOf obo:GO_0019222 , obo:GO_0065007 .

obo:ECO_0001220 a rdfs:Class ;
  rdfs:label "in vivo transcription assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo transcription assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001087 .

obo:GO_0051272 a rdfs:Class ;
  rdfs:label "positive regulation of cellular component movement" ;
  rdfs:subClassOf obo:GO_0048522 , obo:GO_0051270 , obo:GO_0065007 .

obo:ECO_0007845 a rdfs:Class ;
  rdfs:label "radioisotope quantification assay evidence used in automatic assertion" ;
  rdfs:comment "A type of radioisotope quantification assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007455 , obo:ECO_0007844 .

obo:OBI_0000434 a rdfs:Class ;
  rdfs:label "adding material objective"@en ;
  rdfs:comment "is the specification of an objective to add a material into a target material. The adding is asymmetric in the sense that the target material largely retains its identity"@en ;
  rdfs:subClassOf obo:OBI_0000686 .

obo:GO_1901699 a rdfs:Class ;
  rdfs:label "cellular response to nitrogen compound" ;
  rdfs:subClassOf obo:GO_0070887 , obo:GO_1901698 .

obo:OBI_0000115 a rdfs:Class ;
  rdfs:label "intervention design" ;
  rdfs:comment "An intervention design is a study design in which a controlled process applied to the subjects (the intervention) serves as the independent variable manipulated by the experimentalist. The treatment (perturbation or intervention) defined can be defined as a combination of values taken by independent variable manipulated by the experimentalists are applied to the recruited subjects assigned (possibly by applying specific methods) to treatment groups. The specificity of intervention design is the fact that independent variables are being manipulated and a response of the biological system is evaluated via response variables as monitored by possibly a series of assays." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:ECO_0000093 a rdfs:Class ;
  rdfs:label "array-based sequence capture evidence" ;
  rdfs:comment "A type of DNA detection assay evidence evidence where gene sequence or variation is determined or detected by fluorescently-labeled DNA fragments hybridized to sequence-specific oligonucleotides immobilized on an array." ;
  rdfs:subClassOf obo:ECO_0005519 .

obo:ECO_0001030 a rdfs:Class ;
  rdfs:label "conditional knockout evidence" ;
  rdfs:comment "A type of knockout evidence resulting from a temporally-restricted and/or tissue-specific targeted gene removal." ;
  rdfs:subClassOf obo:ECO_0001091 .

obo:ECO_0007324 a rdfs:Class ;
  rdfs:label "no evidence data found used in automatic assertion" ;
  rdfs:comment "An inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000035 , obo:ECO_0007322 .

obo:ECO_0007802 a rdfs:Class ;
  rdfs:label "desumoylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of desumoylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001551 , obo:ECO_0007307 .

obo:ECO_0001249 a rdfs:Class ;
  rdfs:label "fluorescence evidence used in manual assertion" ;
  rdfs:comment "A type of protein detection by fluorescence evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001115 .

obo:ECO_0007217 a rdfs:Class ;
  rdfs:label "protein separation followed by fragment identification evidence used in automatic assertion" ;
  rdfs:comment "A type of protein separation followed by fragment identification evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000160 , obo:ECO_0007787 .

obo:ECO_0007025 a rdfs:Class ;
  rdfs:label "indirect Coombs test evidence" ;
  rdfs:comment "A type of agglutination test evidence that results in screening of antibodies against red blood cell antigens (other than the A and B antigens) that are unbound in a patient's serum in vitro." ;
  rdfs:subClassOf obo:ECO_0007019 .

obo:ECO_0007706 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation evidence used in manual assertion" ;
  rdfs:comment "A type of chromatin immunoprecipitation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000226 , obo:ECO_0005644 .

obo:GO_0009987 a rdfs:Class ;
  rdfs:label "cellular process" ;
  rdfs:comment "Any process that is carried out at the cellular level, but not necessarily restricted to a single cell. For example, cell communication occurs among more than one cell, but occurs at the cellular level." ;
  rdfs:subClassOf obo:GO_0008150 .

obo:GO_0031326 a rdfs:Class ;
  rdfs:label "regulation of cellular biosynthetic process" ;
  rdfs:subClassOf obo:GO_0009889 , obo:GO_0031323 , obo:GO_0065007 .

obo:OBI_0001154 a rdfs:Class ;
  rdfs:label "induced mutagenesis"@en ;
  rdfs:comment "A genetic transformation that is the modification of the genetic material (either coding or non-coding) of an organism caused by mutagenic processes, compounds, or irradiation."@en ;
  rdfs:subClassOf obo:OBI_0600043 .

obo:ECO_0007123 a rdfs:Class ;
  rdfs:label "enzyme inhibition evidence used in manual assertion" ;
  rdfs:comment "A type of enzyme inhibition evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000184 , obo:ECO_0005801 , obo:ECO_0007707 .

obo:ECO_0001268 a rdfs:Class ;
  rdfs:label "TACE activity assay evidence used in manual assertion" ;
  rdfs:comment "A type of TACE activity assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001129 , obo:ECO_0005801 .

obo:OBI_1110108 a rdfs:Class ;
  rdfs:label "material to be added" ;
  rdfs:comment "a material that is added to another one in a material combination process" ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:OBI_0002176 a rdfs:Class ;
  rdfs:label "electrophysiology assay" ;
  rdfs:comment "An assay that measures the electrical properties of biological cells or tissues. Typically, this assay will generate measurements of voltage changes or electric current (or other associated variables such as impedence or capacitance)." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0001058 a rdfs:Class ;
  rdfs:label "in vitro protein cleavage assay evidence" ;
  rdfs:comment "A type of protein cleavage assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001547 .

obo:ECO_0007149 a rdfs:Class ;
  rdfs:label "two-dimensional agarose gel electrophoresis evidence used in manual assertion" ;
  rdfs:comment "A type of two-dimensional agarose gel electrophoresis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0000339 .

obo:ECO_0008055 a rdfs:Class ;
  rdfs:label "antibody CR11-351-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody CR11-351 to determine that HLA protein complex with A2 serotype and/or HLA protein complex with A28 serotype presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0007524 a rdfs:Class ;
  rdfs:label "site-directed mutagenesis phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of site-directed mutagenesis phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005528 , obo:ECO_0007325 .

obo:ECO_0006159 a rdfs:Class ;
  rdfs:label "self-reported patient statement evidence used in manual assertion" ;
  rdfs:comment "A type of self-reported patient statement evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006154 , obo:ECO_0006157 .

obo:ECO_0006377 a rdfs:Class ;
  rdfs:label "quantitative macroscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of quantitative macroscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006343 , obo:ECO_0007430 .

obo:GO_0051179 a rdfs:Class ;
  rdfs:label "localization" ;
  rdfs:subClassOf obo:GO_0008150 .

obo:GO_0043143 a rdfs:Class ;
  rdfs:label "regulation of translation by machinery localization" ;
  rdfs:subClassOf obo:GO_0006417 , obo:GO_0008104 , obo:GO_0034613 .

obo:ECO_0001832 a rdfs:Class ;
  rdfs:label "host colonization assay evidence used in manual assertion" ;
  rdfs:comment "A type of host colonization assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001830 .

obo:ECO_0007554 a rdfs:Class ;
  rdfs:label "single-unit extracellular recording evidence used in automatic assertion" ;
  rdfs:comment "A type of single-unit extracellular recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006045 , obo:ECO_0007553 .

obo:ECO_0007313 a rdfs:Class ;
  rdfs:label "DNA to cDNA expression microarray evidence used in automatic assertion" ;
  rdfs:comment "A type of DNA to cDNA expression microarray evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000104 , obo:ECO_0007301 .

obo:GO_0097659 a rdfs:Class ;
  rdfs:label "nucleic acid-templated transcription" ;
  rdfs:subClassOf obo:GO_0032774 .

obo:ECO_0007765 a rdfs:Class ;
  rdfs:label "reporter gene assay evidence used in automatic assertion" ;
  rdfs:comment "A type of reporter gene assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000049 , obo:ECO_0007298 .

obo:ECO_0007497 a rdfs:Class ;
  rdfs:label "immunofluorescence evidence used in automatic assertion" ;
  rdfs:comment "A type of immunofluorescence evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000007 , obo:ECO_0007819 .

obo:ECO_0007621 a rdfs:Class ;
  rdfs:label "crystal violet staining evidence used in automatic assertion" ;
  rdfs:comment "A type of crystal violet staining evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007069 , obo:ECO_0007798 .

obo:ECO_0005544 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence based on orthology evidence used in manual assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence based on orthology evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005545 , obo:ECO_0005610 .

obo:ECO_0007711 a rdfs:Class ;
  rdfs:label "deubiquitination assay evidence used in manual assertion" ;
  rdfs:comment "A type of deubiquitination assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001552 .

obo:ECO_0001011 a rdfs:Class ;
  rdfs:label "annexin-V staining evidence" ;
  rdfs:comment "A type of apoptotic assay evidence resulting from the addition and subsequent binding of Annexin V to phosphatidylserine in cells to detect if they are viable, apoptotic, or necrotic." ;
  rdfs:subClassOf obo:ECO_0005034 .

obo:ECO_0000361 a rdfs:Class ;
  rdfs:label "inferential evidence" ;
  rdfs:comment "A type of evidence where an assertion is derived from another assertion via logical inference or some non-logical but rational means." ;
  rdfs:subClassOf obo:ECO_0000000 .

obo:GO_1904171 a rdfs:Class ;
  rdfs:label "negative regulation of bleb assembly" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_0120033 , obo:GO_1904170 .

obo:ECO_0001578 a rdfs:Class ;
  rdfs:label "spheroplast fusion assay evidence used in automatic assertion" ;
  rdfs:comment "A type of spheroplast fusion assay evidence used in automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001575 , obo:ECO_0001577 .

obo:ECO_0008048 a rdfs:Class ;
  rdfs:label "antibody B123.2-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody B123.2 to determine that the human MHC class I protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0000082 a rdfs:Class ;
  rdfs:label "obsolete polymerase chain reaction evidence" ;
  rdfs:comment "OBSOLETE. A type of experimental genomic evidence where DNA polymerase is used to synthesize new strand of DNA complementary to the offered template strand." .

obo:ECO_0000346 a rdfs:Class ;
  rdfs:label "support of intron positions by RNA-sequencing alignment evidence used in manual assertion" ;
  rdfs:comment "A type of support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000342 , obo:ECO_0006068 .

obo:ECO_0001584 a rdfs:Class ;
  rdfs:label "small interfering RNA knockdown evidence used in automatic assertion" ;
  rdfs:comment "A type of small interfering RNA knockdown evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001583 , obo:ECO_0007556 .

obo:ECO_0001115 a rdfs:Class ;
  rdfs:label "fluorescence evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from the analysis of a molecule by its intrinsic fluorescence, or by attaching it with a fluorophore." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007098 a rdfs:Class ;
  rdfs:label "morphological similarity evidence used in manual assertion" ;
  rdfs:comment "A type of morphological similarity evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000071 , obo:ECO_0000252 .

obo:ECO_0007689 a rdfs:Class ;
  rdfs:label "sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence used in manual assertion" ;
  rdfs:comment "A type of sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0000333 .

obo:ECO_0006090 a rdfs:Class ;
  rdfs:label "protein-oligonucleotide microarray binding evidence used in manual assertion" ;
  rdfs:comment "A type of protein-oligonucleotide microarray binding evidence that is used in a manual assertion that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites." ;
  rdfs:subClassOf obo:ECO_0001601 , obo:ECO_0006076 , obo:ECO_0006083 .

obo:ECO_0008197 a rdfs:Class ;
  rdfs:label "single-molecule fluorescence resonance energy transfer evidence used in automatic assertion" ;
  rdfs:comment "A type of single-molecule fluorescence resonance energy transfer evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007387 , obo:ECO_0008187 .

obo:ECO_0007088 a rdfs:Class ;
  rdfs:label "ribosome profiling evidence used in manual assertion" ;
  rdfs:comment "A type of ribosome profiling evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000270 , obo:ECO_0007087 .

obo:ECO_0007363 a rdfs:Class ;
  rdfs:label "imaging assay evidence used in automatic assertion" ;
  rdfs:comment "A type of imaging assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000324 , obo:ECO_0007307 .

obo:GO_0046903 a rdfs:Class ;
  rdfs:label "secretion" ;
  rdfs:subClassOf obo:GO_0006810 .

obo:ECO_0007048 a rdfs:Class ;
  rdfs:label "time-lapsed microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of time-lapsed microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001232 , obo:ECO_0007047 .

obo:ECO_0008029 a rdfs:Class ;
  rdfs:label "inference of intermediate molecular structure from structure of final molecule used in automatic assertion" ;
  rdfs:comment "A type of inference of intermediate molecular structure from structure of final molecule that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007322 , obo:ECO_0008015 .

obo:ECO_0006045 a rdfs:Class ;
  rdfs:label "single-unit extracellular recording evidence" ;
  rdfs:comment "A type of extracellular recording evidence in which an extracellular microelectrode is used to measure the electrical activity of a single neuron." ;
  rdfs:subClassOf obo:ECO_0006043 .

obo:ECO_0000335 a rdfs:Class ;
  rdfs:label "substance quantification evidence" ;
  rdfs:comment "A type of direct assay evidence where the quantity of a substance used as part of an assay is measured." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0001073 a rdfs:Class ;
  rdfs:label "in vitro ubiquitination assay evidence" ;
  rdfs:comment "A type of ubiquitination assay evidenceused in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001562 .

obo:ECO_0006058 a rdfs:Class ;
  rdfs:label "high throughput cell biology evidence" ;
  rdfs:comment "A type of high throughput evidence where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel." ;
  rdfs:subClassOf obo:ECO_0006055 .

obo:ECO_0005007 a rdfs:Class ;
  rdfs:label "cell proliferation assay evidence" ;
  rdfs:comment "A type of cell-based assay evidence resulting from the measurement of the number of cells, which is a reflection of the balance between cell division and cell loss through cell differentiation or cell death." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:ECO_0001175 a rdfs:Class ;
  rdfs:label "deletion mutation phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of deletion mutation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0001038 .

obo:ECO_0001104 a rdfs:Class ;
  rdfs:label "nuclear fragmentation evidence" ;
  rdfs:comment "A type of apoptotic assay evidence resulting from the nuclei fragmenting into smaller pieces during programmed cell death." ;
  rdfs:subClassOf obo:ECO_0005034 .

obo:OBI_0002187 a rdfs:Class ;
  rdfs:label "single-unit recording" ;
  rdfs:comment "An extracellular electrophysiology assay where a single microelectrode is placed in close proximity to a single neuron to measure voltage and current changes over time. This is the technicque used by Hubel and Wiesel to measure firing properties of primary visual cortex neurons in the 1950s in their original Nobel-prize winning study. A classic, old technique." ;
  rdfs:subClassOf obo:OBI_0000454 , obo:OBI_0001977 .

obo:GO_2000193 a rdfs:Class ;
  rdfs:label "positive regulation of fatty acid transport" ;
  rdfs:subClassOf obo:GO_0032370 , obo:GO_0032892 , obo:GO_0065007 , obo:GO_2000191 .

obo:ECO_0000100 a rdfs:Class ;
  rdfs:label "fractionation evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from the separation of various cell components while preserving their individual functions." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007567 a rdfs:Class ;
  rdfs:label "co-purification evidence used in automatic assertion" ;
  rdfs:comment "A type of co-purification evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000022 , obo:ECO_0007332 .

obo:ECO_0007018 a rdfs:Class ;
  rdfs:label "photoconversion evidence used in manual assertion" ;
  rdfs:comment "A type of photoconversion evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001249 , obo:ECO_0007017 .

obo:ECO_0008149 a rdfs:Class ;
  rdfs:label "antibody OX18-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody OX18-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008070 , obo:ECO_0008087 .

obo:ECO_0005515 a rdfs:Class ;
  rdfs:label "translocation mutation phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence resulting from a mutation in which a region of a chromosome is transferred to a nonhomologous chromosome." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0005645 a rdfs:Class ;
  rdfs:label "interferometric reflectance imaging sensor evidence used in manual assertion" ;
  rdfs:comment "A type of interferometric reflectance imaging sensor evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0005520 .

obo:ECO_0001156 a rdfs:Class ;
  rdfs:label "caspase assay evidence used in manual assertion" ;
  rdfs:comment "A type of caspase assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001019 , obo:ECO_0007698 .

obo:ECO_0006362 a rdfs:Class ;
  rdfs:label "intrinsic fluorescence-based protein conformation evidence used in manual assertion" ;
  rdfs:comment "A type of intrinsic fluorescence-based protein conformation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001249 , obo:ECO_0006336 .

obo:ECO_0006405 a rdfs:Class ;
  rdfs:label "experimentally evolved mutant phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of experimentally evolved mutant phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006404 , obo:ECO_0007325 .

obo:ECO_0007751 a rdfs:Class ;
  rdfs:label "inferential evidence used in manual assertion" ;
  rdfs:comment "A type of inferential evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000352 , obo:ECO_0000361 .

obo:ECO_0005672 a rdfs:Class ;
  rdfs:label "xylE reporter gene assay evidence used in manual assertion" ;
  rdfs:comment "A type of xylE reporter gene assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000270 , obo:ECO_0005537 .

obo:GO_0034248 a rdfs:Class ;
  rdfs:label "regulation of cellular amide metabolic process" ;
  rdfs:subClassOf obo:GO_0031323 , obo:GO_0051171 , obo:GO_0065007 .

obo:ECO_0001186 a rdfs:Class ;
  rdfs:label "immunocytochemistry evidence used in manual assertion" ;
  rdfs:comment "A type of immunocytochemistry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001053 .

obo:GO_0004518 a rdfs:Class ;
  rdfs:label "nuclease activity" ;
  rdfs:subClassOf obo:GO_0016788 .

obo:GO_1901700 a rdfs:Class ;
  rdfs:label "response to oxygen-containing compound" ;
  rdfs:subClassOf obo:GO_0042221 .

obo:GO_0034655 a rdfs:Class ;
  rdfs:label "nucleobase-containing compound catabolic process" ;
  rdfs:subClassOf obo:GO_0006139 , obo:GO_0019439 , obo:GO_0044270 , obo:GO_0046700 , obo:GO_1901361 .

obo:ECO_0007301 a rdfs:Class ;
  rdfs:label "expression microarray evidence used in automatic assertion" ;
  rdfs:comment "A type of expression microarray evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000058 , obo:ECO_0007303 .

obo:ECO_0005520 a rdfs:Class ;
  rdfs:label "interferometric reflectance imaging sensor evidence" ;
  rdfs:comment "A type of molecule detection assay evidence based on changes in surface-reflected light due to ligand binding on the surface of a semi-transparent substrate to detect proteins, DNA, and other biological material in a multiplexed, high-throughput microarray format without labels." ;
  rdfs:subClassOf obo:ECO_0005516 .

obo:OBI_0001234 a rdfs:Class ;
  rdfs:label "epigenetic modification identification objective"@en ;
  rdfs:comment "A molecular feature identification objective that aims to detect epigenetic modifications, such as DNA methylation, histone modifications."@en ;
  rdfs:subClassOf obo:OBI_0000131 .

obo:ECO_0001833 a rdfs:Class ;
  rdfs:label "infection assay evidence" ;
  rdfs:comment "A type of host colonization assay evidence in which the infection capacity is measured by assessing changes in the host." ;
  rdfs:subClassOf obo:ECO_0001830 .

obo:GO_0022898 a rdfs:Class ;
  rdfs:label "regulation of transmembrane transporter activity" ;
  rdfs:subClassOf obo:GO_0032409 , obo:GO_0034762 , obo:GO_0065007 .

obo:GO_0008104 a rdfs:Class ;
  rdfs:label "protein localization" ;
  rdfs:subClassOf obo:GO_0033036 .

obo:ECO_0007514 a rdfs:Class ;
  rdfs:label "hydroxyl-radical footprinting evidence used in automatic assertion" ;
  rdfs:comment "A type of hydroxyl-radical footprinting evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001818 , obo:ECO_0007505 .

obo:CL_0000542 a rdfs:Class ;
  rdfs:label "lymphocyte" ;
  rdfs:comment "A lymphocyte is a leukocyte commonly found in the blood and lymph that has the characteristics of a large nucleus, a neutral staining cytoplasm, and prominent heterochromatin." ;
  rdfs:subClassOf obo:CL_0000842 .

obo:ECO_0006351 a rdfs:Class ;
  rdfs:label "bait-prey interaction evidence" ;
  rdfs:comment "A type of protein binding evidence in which the use of \"bait\" molecules or proteins allows the purification or identification of a binding partner, the \"prey\"." ;
  rdfs:subClassOf obo:ECO_0000024 .

obo:OBI_0001441 a rdfs:Class ;
  rdfs:label "proteomic profiling by array design" ;
  rdfs:comment "A study design in which proteins in a sample are detected, quantified or otherwise analysed, through an array-based technology." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:ECO_0000003 a rdfs:Class ;
  rdfs:label "reconstitution assay evidence" ;
  rdfs:comment "A type of direct assay evidence based on reconstructing a biological sample from its disassociated state to its original state." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0006272 a rdfs:Class ;
  rdfs:label "viscosity measurement evidence" ;
  rdfs:comment "A type of direct assay evidence where the viscosity of a solution is measured to quantify the material properties of the solution, or the changes of these properties in response to changing composition or environmental factors." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0008089 a rdfs:Class ;
  rdfs:label "antibody 10.3.6-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody 10.3.6-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008040 , obo:ECO_0008087 .

obo:OBI_0302903 a rdfs:Class ;
  rdfs:label "nucleic acid hybridization"@en ;
  rdfs:comment "a planned process by which totally or partially complementary, single-stranded nucleic acids are  combined into a single molecule called heteroduplex or homoduplex to an extent depending on the amount of complementarity."@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0007062 a rdfs:Class ;
  rdfs:label "light scattering evidence used in manual assertion" ;
  rdfs:comment "A type of light scattering evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0007061 .

obo:CHEBI_472552 a rdfs:Class ;
  rdfs:label "5-bromo-2'-deoxyuridine" ;
  rdfs:comment "A pyrimidine 2'-deoxyribonucleoside compound having 5-bromouracil as the nucleobase." ;
  rdfs:subClassOf obo:CHEBI_23367 .

obo:OBI_0000834 a rdfs:Class ;
  rdfs:label "high molecular weight DNA extract" ;
  rdfs:comment "The output of an extraction process in which DNA molecules above a molecular weight cutoff are purified in order to exclude DNA from organellas." ;
  rdfs:subClassOf obo:OBI_0001051 .

obo:ECO_0006232 a rdfs:Class ;
  rdfs:label "differential scanning calorimetry evidence used in manual assertion" ;
  rdfs:comment "A type of differential scanning calorimetry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006193 , obo:ECO_0006230 .

obo:ECO_0007189 a rdfs:Class ;
  rdfs:label "obsolete inference from experimental data evidence used in manual assertion" ;
  rdfs:comment "OBSOLETE. A type of inference from experimental data evidence that is used in a manual assertion." .

obo:OBI_0001632 a rdfs:Class ;
  rdfs:label "immuno staining assay" ;
  rdfs:comment "A assay detecting a molecular label assay in which the label is attached to an antibody so that substances are marked based on the antibody's binding specificity." ;
  rdfs:subClassOf obo:OBI_0600017 .

obo:ECO_0005628 a rdfs:Class ;
  rdfs:label "DNA adenine methyltransferase identification evidence used in automatic assertion" ;
  rdfs:comment "A type of DNA adenine methyltransferase identification evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001824 , obo:ECO_0007384 .

obo:ECO_0007781 a rdfs:Class ;
  rdfs:label "cytotoxicity assay evidence used in automatic assertion" ;
  rdfs:comment "A type of cytotoxicity assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005012 , obo:ECO_0007773 .

obo:ECO_0001562 a rdfs:Class ;
  rdfs:label "ubiquitination assay evidence" ;
  rdfs:comment "A type of direct assay evidence where ubiquitinated residues on a protein are detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0001055 a rdfs:Class ;
  rdfs:label "immunohistochemistry evidence" ;
  rdfs:comment "A type of protein detection assay evidence resulting from the use of antibodies to detect proteins in localized cells of tissue sections." ;
  rdfs:subClassOf obo:ECO_0005517 .

obo:ECO_0000021 a rdfs:Class ;
  rdfs:label "physical interaction evidence" ;
  rdfs:comment "A type of experimental evidence that is based on characterization of an interaction between a gene product and another molecule." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0005634 a rdfs:Class ;
  rdfs:label "genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion" ;
  rdfs:comment "A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001812 , obo:ECO_0006088 .

obo:ECO_0008053 a rdfs:Class ;
  rdfs:label "antibody BB7.2-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody BB7.2 to determine that HLA protein complex with A2 serotype presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0007341 a rdfs:Class ;
  rdfs:label "adoptive cell transfer evidence used in automatic assertion" ;
  rdfs:comment "A type of adoptive cell transfer evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001007 , obo:ECO_0007793 .

obo:ECO_0006315 a rdfs:Class ;
  rdfs:label "pH-induced protein unfolding evidence used in manual assertion" ;
  rdfs:comment "A type of pH-induced protein unfolding evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006267 , obo:ECO_0006311 .

obo:ECO_0007144 a rdfs:Class ;
  rdfs:label "cap analysis of gene expression evidence used in manual assertion" ;
  rdfs:comment "A type of cap analysis of gene expression evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000291 , obo:ECO_0000309 .

obo:OBI_0002165 a rdfs:Class ;
  rdfs:label "protein localization assay" ;
  rdfs:comment "An assay that determines the specific location of a protein. Subcellular localization is distinguished from tissue-based localization based on the type of microscopy applied." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007224 a rdfs:Class ;
  rdfs:label "animal model system study evidence used in automatic assertion" ;
  rdfs:comment "A type of animal model system study evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000179 , obo:ECO_0007297 .

obo:OBI_0600054 a rdfs:Class ;
  rdfs:label "selection by survival"@en ;
  rdfs:comment "the use of environmental conditions to select for the organism or cells that have a certain trait"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:PATO_0001740 a rdfs:Class ;
  rdfs:label "activity (of a radionuclide)" ;
  rdfs:comment "A radiation quality inhering in a radioactive substance by virtue of its transformation (disintegration) rate." ;
  rdfs:subClassOf obo:PATO_0001739 .

obo:GO_1901576 a rdfs:Class ;
  rdfs:label "organic substance biosynthetic process" ;
  rdfs:subClassOf obo:GO_0009058 , obo:GO_0071704 .

obo:ECO_0007293 a rdfs:Class ;
  rdfs:label "obsolete inference from experimental data evidence used in automatic assertion" ;
  rdfs:comment "OBSOLETE. A type of inference from experimental data evidence that is used in an automatic assertion." .

obo:ECO_0001199 a rdfs:Class ;
  rdfs:label "in vitro palmitoylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro palmitoylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001066 .

obo:ECO_0007691 a rdfs:Class ;
  rdfs:label "protein cleavage assay evidence used in manual assertion" ;
  rdfs:comment "A type of protein cleavage assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001547 .

obo:OBI_0001709 a rdfs:Class ;
  rdfs:label "assay measuring a binding constant of a B cell epitope:antibody complex" ;
  rdfs:comment "A B cell epitope recognition assay that quantitavely characterizes the binding of an antibody / BCR with a ligand by determining a binding constant. " ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_0003146 .

obo:GO_0070887 a rdfs:Class ;
  rdfs:label "cellular response to chemical stimulus" ;
  rdfs:subClassOf obo:GO_0042221 , obo:GO_0051716 .

obo:GO_0080134 a rdfs:Class ;
  rdfs:label "regulation of response to stress" ;
  rdfs:subClassOf obo:GO_0048583 , obo:GO_0065007 .

obo:OBI_0000411 a rdfs:Class ;
  rdfs:label "cloning vector role"@en ;
  rdfs:comment "A material to be added role played by a small, self-replicating DNA or RNA molecule - usually a plasmid or chromosome - and realized in a process whereby foreign DNA or RNA is inserted into the vector during the process of cloning."@en ;
  rdfs:subClassOf obo:OBI_0000319 .

obo:OBI_0000379 a rdfs:Class ;
  rdfs:label "mechanical function"@en ;
  rdfs:comment "A mechanical function is a function that is realised via mechanical work (through an certain amount of energy transferred by some force)."@en ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:ECO_0006322 a rdfs:Class ;
  rdfs:label "RNA-protein binding evidence used in automatic assertion" ;
  rdfs:comment "A type of RNA-protein binding evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006270 , obo:ECO_0007373 , obo:ECO_0007505 .

obo:OBI_0001917 a rdfs:Class ;
  rdfs:label "chromosome conformation identification objective"@en ;
  rdfs:comment "A molecular feature identification objective that aims to determine spatial organization of chromatin." ;
  rdfs:subClassOf obo:OBI_0000131 .

obo:GO_0000737 a rdfs:Class ;
  rdfs:label "DNA catabolic process, endonucleolytic" ;
  rdfs:subClassOf obo:GO_0006308 , obo:GO_0090305 .

obo:ECO_0000285 a rdfs:Class ;
  rdfs:label "DNA to cDNA expression microarray evidence used in manual assertion" ;
  rdfs:comment "A type of DNA to cDNA expression microarray evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000104 , obo:ECO_0000275 .

obo:ECO_0000044 a rdfs:Class ;
  rdfs:label "sequence similarity evidence" ;
  rdfs:comment "A type of similarity based on biomolecular sequence." ;
  rdfs:subClassOf obo:ECO_0000041 , obo:ECO_0007672 .

obo:GO_2000113 a rdfs:Class ;
  rdfs:label "negative regulation of cellular macromolecule biosynthetic process" ;
  rdfs:subClassOf obo:GO_0010558 , obo:GO_0031327 , obo:GO_0065007 , obo:GO_2000112 .

obo:GO_0051247 a rdfs:Class ;
  rdfs:label "positive regulation of protein metabolic process" ;
  rdfs:subClassOf obo:GO_0010604 , obo:GO_0051173 , obo:GO_0051246 , obo:GO_0065007 .

obo:ECO_0007000 a rdfs:Class ;
  rdfs:label "high throughput mutant phenotypic evidence" ;
  rdfs:comment "A type of high throughput evidence derived from high throughput analysis of differences in alleles of a corresponding gene." ;
  rdfs:subClassOf obo:ECO_0000015 , obo:ECO_0006055 .

obo:ECO_0007374 a rdfs:Class ;
  rdfs:label "crystallography evidence used in automatic assertion" ;
  rdfs:comment "A type of crystallography evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001034 , obo:ECO_0007815 .

obo:ECO_0000060 a rdfs:Class ;
  rdfs:label "positional similarity evidence" ;
  rdfs:comment "A type of phenotypic similarity evidence based on the similarity of stucture locations or arrangements." ;
  rdfs:subClassOf obo:ECO_0000057 .

obo:ECO_0000270 a rdfs:Class ;
  rdfs:label "expression pattern evidence used in manual assertion" ;
  rdfs:comment "A type of expression pattern evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/iep-inferred-expression-pattern> ;
  rdfs:subClassOf obo:ECO_0000008 , obo:ECO_0000269 .

obo:GO_0042246 a rdfs:Class ;
  rdfs:label "tissue regeneration" ;
  rdfs:subClassOf obo:GO_0031099 , obo:GO_0048589 .

obo:OBI_0000686 a rdfs:Class ;
  rdfs:label "material combination objective" ;
  rdfs:comment "is an objective to obtain an output material that contains several input materials." ;
  rdfs:subClassOf obo:OBI_0000456 .

obo:GO_0065008 a rdfs:Class ;
  rdfs:label "regulation of biological quality" ;
  rdfs:subClassOf obo:GO_0065007 .

obo:OBI_0000151 a rdfs:Class ;
  rdfs:label "filter paper" ;
  rdfs:comment "A device manufacture with the intent to provide a porous unsized paper used for filtering." ;
  rdfs:subClassOf obo:OBI_0000932 .

obo:GO_0009892 a rdfs:Class ;
  rdfs:label "negative regulation of metabolic process" ;
  rdfs:subClassOf obo:GO_0019222 , obo:GO_0048519 , obo:GO_0065007 .

obo:GO_1900132 a rdfs:Class ;
  rdfs:label "positive regulation of lipid binding" ;
  rdfs:subClassOf obo:GO_0051099 , obo:GO_0065007 , obo:GO_1900130 .

obo:ECO_0007455 a rdfs:Class ;
  rdfs:label "radioisotope assay evidence used in automatic assertion" ;
  rdfs:comment "A type of radioisotope assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001120 , obo:ECO_0007307 .

obo:GO_0044270 a rdfs:Class ;
  rdfs:label "cellular nitrogen compound catabolic process" ;
  rdfs:subClassOf obo:GO_0034641 , obo:GO_0044248 .

obo:GO_0032079 a rdfs:Class ;
  rdfs:label "positive regulation of endodeoxyribonuclease activity" ;
  rdfs:subClassOf obo:GO_0032071 , obo:GO_0032077 , obo:GO_0065007 .

obo:ECO_0000245 a rdfs:Class ;
  rdfs:label "automatically integrated combinatorial evidence used in manual assertion" ;
  rdfs:comment "A type of automatically integrated combinatorial evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/rca-inferred-reviewed-computational-analysis> ;
  rdfs:subClassOf obo:ECO_0000244 , obo:ECO_0007673 .

obo:ECO_0006292 a rdfs:Class ;
  rdfs:label "deglycosylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of deglycosylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006255 , obo:ECO_0007307 .

obo:ECO_0000323 a rdfs:Class ;
  rdfs:label "imported automatically asserted information used in automatic assertion" ;
  rdfs:comment "A type of imported information used in automatic assertion that was initially used in automatic assertion by one person or group, which is imported by a second person or group and used in automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000313 .

obo:GO_0032309 a rdfs:Class ;
  rdfs:label "icosanoid secretion" ;
  rdfs:subClassOf obo:GO_0046903 , obo:GO_0071715 .

obo:ECO_0007604 a rdfs:Class ;
  rdfs:label "copper transport assay evidence used in automatic assertion" ;
  rdfs:comment "A type of copper transport assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007035 , obo:ECO_0007789 .

obo:ECO_0000298 a rdfs:Class ;
  rdfs:label "cleavage arrested development evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence where embryonic development proceeds although cytokinesis is blocked." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0005526 a rdfs:Class ;
  rdfs:label "matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence" ;
  rdfs:comment "A type of protein detection assay evidence where soft ionization is used for the analysis of biomolecules for mass determination in a three step process of plate preparation, radiation, and ionization with high throughput technology." ;
  rdfs:subClassOf obo:ECO_0005517 .

obo:ECO_0007796 a rdfs:Class ;
  rdfs:label "immunodetection assay evidence used in automatic assertion" ;
  rdfs:comment "A type of immunodetection assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005593 , obo:ECO_0007307 .

obo:ECO_0007385 a rdfs:Class ;
  rdfs:label "filter binding assay evidence used in automatic assertion" ;
  rdfs:comment "A type of filter binding assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001046 , obo:ECO_0007332 .

obo:ECO_0007542 a rdfs:Class ;
  rdfs:label "microtiter plate biofilm assay evidence used in automatic assertion" ;
  rdfs:comment "A type of microtiter plate biofilm assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006022 , obo:ECO_0007541 .

obo:ECO_0007475 a rdfs:Class ;
  rdfs:label "reverse transcription polymerase chain reaction evidence used in automatic assertion" ;
  rdfs:comment "A type of reverse transcription polymerase chain reaction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000109 , obo:ECO_0007818 .

obo:ECO_0001522 a rdfs:Class ;
  rdfs:label "small molecule detection assay evidence" ;
  rdfs:comment "A type of molecule detection assay evidence resulting from the detection and quantification of a small molecule (a low molecular weight (typically < 900 daltons) organic compound), such as lipids, sugars, animo acids, drugs, etc." ;
  rdfs:subClassOf obo:ECO_0005516 .

obo:GO_1904170 a rdfs:Class ;
  rdfs:label "regulation of bleb assembly" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_0120032 .

obo:CHEBI_33839 a rdfs:Class ;
  rdfs:label "macromolecule" ;
  rdfs:comment "A macromolecule is a molecule of high relative molecular mass, the structure of which essentially comprises the multiple repetition of units derived, actually or conceptually, from molecules of low relative molecular mass." ;
  rdfs:subClassOf obo:CHEBI_23367 .

obo:OBI_0000470 a rdfs:Class ;
  rdfs:label "mass spectrometry assay" ;
  rdfs:comment "An assay that identifies the amount and type of material entities present in a sample by fragmenting the sample and measuring the mass-to-charge ratio of the resulting particles." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007437 a rdfs:Class ;
  rdfs:label "MTS assay evidence used in automatic assertion" ;
  rdfs:comment "A type of MTS assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001100 , obo:ECO_0007777 .

obo:GO_0051052 a rdfs:Class ;
  rdfs:label "regulation of DNA metabolic process" ;
  rdfs:subClassOf obo:GO_0019219 , obo:GO_0060255 , obo:GO_0065007 .

obo:CHEBI_33696 a rdfs:Class ;
  rdfs:label "nucleic acid" ;
  rdfs:comment "A macromolecule made up of nucleotide units and hydrolysable into certain pyrimidine or purine bases (usually adenine, cytosine, guanine, thymine, uracil), D-ribose or 2-deoxy-D-ribose and phosphoric acid." ;
  rdfs:subClassOf obo:CHEBI_33839 .

obo:ECO_0008042 a rdfs:Class ;
  rdfs:label "antibody 28-14-8S-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody 28-14-8S to determine that the H2-Ld protein complex, H2-Db protein complex, and/or H2-Dq protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:GO_0032069 a rdfs:Class ;
  rdfs:label "regulation of nuclease activity" ;
  rdfs:subClassOf obo:GO_0051336 , obo:GO_0060255 , obo:GO_0065007 .

obo:ECO_0007466 a rdfs:Class ;
  rdfs:label "tissue microarray evidence used in automatic assertion" ;
  rdfs:comment "A type of tissue microarray evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001130 , obo:ECO_0007375 .

obo:ECO_0007051 a rdfs:Class ;
  rdfs:label "transmitted light brightfied mircoscopy evidence" ;
  rdfs:comment "A type of microscopy evidence where a sample is illuminated with transmitted white light from below and observed from above." ;
  rdfs:subClassOf obo:ECO_0001098 .

obo:ECO_0005027 a rdfs:Class ;
  rdfs:label "genetic transformation evidence" ;
  rdfs:comment "A type of mutant phenotype evidence resulting from the conversion of one genotype into another by the introduction of exogenous DNA." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0007041 a rdfs:Class ;
  rdfs:label "epifluorescence microscopy evidence" ;
  rdfs:comment "A type of fluorescence microscopy evidence where the light source is mounted above the specimen and the excitation light passes through the microscope objective lens on its way toward the specimen." ;
  rdfs:subClassOf obo:ECO_0006271 .

obo:GO_0051222 a rdfs:Class ;
  rdfs:label "positive regulation of protein transport" ;
  rdfs:subClassOf obo:GO_0051050 , obo:GO_0051223 , obo:GO_0065007 , obo:GO_1904951 .

obo:ECO_0001163 a rdfs:Class ;
  rdfs:label "co-localization evidence used in manual assertion" ;
  rdfs:comment "A type of co-localization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001026 , obo:ECO_0001256 .

obo:ECO_0007178 a rdfs:Class ;
  rdfs:label "reconstituted bilayer single-channel patch recording evidence used in manual assertion" ;
  rdfs:comment "A type of reconstituted bilayer single-channel patch recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005575 , obo:ECO_0006013 .

obo:OBI_0001153 a rdfs:Class ;
  rdfs:label "genetic transformation objective"@en ;
  rdfs:comment "a material transformation objective aims to create genetically modified organism or cell"@en ;
  rdfs:subClassOf obo:OBI_0000456 .

obo:ECO_0007029 a rdfs:Class ;
  rdfs:label "viral hemagglutination inhibition assay evidence" ;
  rdfs:comment "A type of agglutination test evidence where antiviral antibodies in a patient's serum or in a lab-produced antiserum neutralize the virus and block it from agglutinating the red blood cells." ;
  rdfs:subClassOf obo:ECO_0007019 .

obo:ECO_0000010 a rdfs:Class ;
  rdfs:label "protein expression evidence" ;
  rdfs:comment "A type of expression pattern evidence resulting from protein abundance quantification techniques." ;
  rdfs:subClassOf obo:ECO_0000008 .

obo:ECO_0001020 a rdfs:Class ;
  rdfs:label "cell counting evidence" ;
  rdfs:comment "A type of cell-based assay evidence resulting from the quantification of a sample of cells." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:OBI_0003043 a rdfs:Class ;
  rdfs:label "urine assay" ;
  rdfs:comment "An assay that has a urine specimen as evaluant." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:OBI_0000737 a rdfs:Class ;
  rdfs:label "cloning vector" ;
  rdfs:comment "A cloning vector is an engineered material that is used as an input material for a recombinant vector cloning process to carry inserted nucleic acids. It contains an origin of replication for a specific destination host organism, encodes for a selectable gene product and contains a cloning site." ;
  rdfs:subClassOf obo:BFO_0000040 , obo:OBI_0000047 , obo:OBI_1110108 .

obo:ECO_0005500 a rdfs:Class ;
  rdfs:label "in vivo polyADP-ribosylation assay evidence" ;
  rdfs:comment "A type of polyADP-ribosylation assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001557 .

obo:ECO_0006221 a rdfs:Class ;
  rdfs:label "X-ray crystallography-based structural model with missing residue coordinates used in automatic assertion" ;
  rdfs:comment "A type of X-ray crystallography-based structural model with missing residue coordinates that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005671 , obo:ECO_0006187 .

obo:ECO_0007770 a rdfs:Class ;
  rdfs:label "sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence used in automatic assertion" ;
  rdfs:comment "A type of sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000333 , obo:ECO_0007769 .

obo:ECO_0001180 a rdfs:Class ;
  rdfs:label "eTag assay evidence used in manual assertion" ;
  rdfs:comment "A type of eTag assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001045 , obo:ECO_0006075 .

obo:ECO_0007509 a rdfs:Class ;
  rdfs:label "ferric uptake regulator titration assay evidence used in automatic assertion" ;
  rdfs:comment "A type of ferric uptake regulator titration assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001829 , obo:ECO_0007765 .

obo:ECO_0001044 a rdfs:Class ;
  rdfs:label "Edman degradation evidence" ;
  rdfs:comment "A type of protein detection assay evidence resulting from labeling a termonal amino acid residue and cleaving it from a peptide by sequentially removing one residue at a time from the amino end of the peptide, without disrupting the bonds." ;
  rdfs:subClassOf obo:ECO_0005517 .

obo:ECO_0007133 a rdfs:Class ;
  rdfs:label "3C-qPCR evidence used in manual assertion" ;
  rdfs:comment "A type of 3C-qPCR evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000237 , obo:ECO_0000269 .

obo:ECO_0000312 a rdfs:Class ;
  rdfs:label "imported information used in manual assertion" ;
  rdfs:comment "A type of imported information that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000311 , obo:ECO_0006155 .

obo:OBI_0001668 a rdfs:Class ;
  rdfs:label "split-ubiquitin assay" ;
  rdfs:comment "A binding assay that screens membrane soluble proteins by fusion of two integral membrane proteins (bait and prey) to two different ubiquitin moieties. One moiety is also fused to a transcription factor (TF) that can be cleaved off by ubiquitin specific proteases. Upon bait and prey interaction, Nub and Cub-moieties assemble, reconstituting the split-ubiquitin. The reconstituted split-ubiquitin molecule is recognized by ubiquitin specific proteases, which cleave off the reporter protein, allowing it to induce the transcription of reporter genes." ;
  rdfs:subClassOf obo:OBI_0001146 .

obo:GO_1901566 a rdfs:Class ;
  rdfs:label "organonitrogen compound biosynthetic process" ;
  rdfs:subClassOf obo:GO_1901564 , obo:GO_1901576 .

obo:GO_0019219 a rdfs:Class ;
  rdfs:label "regulation of nucleobase-containing compound metabolic process" ;
  rdfs:subClassOf obo:GO_0031323 , obo:GO_0051171 , obo:GO_0065007 , obo:GO_0080090 .

obo:ECO_0005627 a rdfs:Class ;
  rdfs:label "DNA adenine methyltransferase identification evidence used in manual assertion" ;
  rdfs:comment "A type of DNA adenine methyltransferase identification evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001824 , obo:ECO_0006075 .

obo:ECO_0001847 a rdfs:Class ;
  rdfs:label "cell population optical density evidence used in manual assertion" ;
  rdfs:comment "A type of cell population optical density evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001845 , obo:ECO_0007695 .

obo:GO_0006309 a rdfs:Class ;
  rdfs:label "apoptotic DNA fragmentation" ;
  rdfs:subClassOf obo:GO_0000737 .

obo:ECO_0007030 a rdfs:Class ;
  rdfs:label "viral hemagglutination inhibition assay evidence used in manual assertion" ;
  rdfs:comment "A type of viral hemagglutination inhibition assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007020 , obo:ECO_0007029 .

obo:ECO_0006030 a rdfs:Class ;
  rdfs:label "co-immunoprecipitation evidence used in manual assertion" ;
  rdfs:comment "A type of co-immunoprecipitation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000070 , obo:ECO_0005644 .

obo:ECO_0005543 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005552 , obo:ECO_0007747 .

obo:OBI_0001430 a rdfs:Class ;
  rdfs:label "transcription profiling design" ;
  rdfs:comment "A study design that identifies forms and abundance of transcripts in the genome." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:ECO_0007357 a rdfs:Class ;
  rdfs:label "cell permeability assay evidence used in automatic assertion" ;
  rdfs:comment "A type of cell permeability assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001021 , obo:ECO_0007773 .

obo:ECO_0005548 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence based on inference from background scientific knowledge" ;
  rdfs:comment "A type of biological system reconstruction based solely on background scientific knowledge of that biological system." ;
  rdfs:subClassOf obo:ECO_0000088 .

obo:OBI_0400148 a rdfs:Class ;
  rdfs:label "DNA microarray" ;
  rdfs:comment "A DNA-microarray is a microarray that is used as a physical 2D immobilisation matrix for DNA sequences. DNA microarray-bound DNA fragments are used as targets for a hybridising probed sample." ;
  rdfs:subClassOf obo:OBI_0400147 .

obo:ECO_0007589 a rdfs:Class ;
  rdfs:label "high throughput direct assay evidence used in automatic assertion" ;
  rdfs:comment "A type of high throughput direct assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006057 , obo:ECO_0007004 , obo:ECO_0007307 .

obo:ECO_0008064 a rdfs:Class ;
  rdfs:label "antibody L243-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody L243 to determine that the HLA-DR protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0007626 a rdfs:Class ;
  rdfs:label "colony color phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of colony color phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007079 , obo:ECO_0007625 .

obo:GO_0044070 a rdfs:Class ;
  rdfs:label "regulation of anion transport" ;
  rdfs:subClassOf obo:GO_0043269 , obo:GO_0065007 .

obo:ECO_0007755 a rdfs:Class ;
  rdfs:label "curator inference from MedlinePlus encyclopedia used in manual assertion" ;
  rdfs:comment "A type of curator inference from MedlinePlus encyclopedia that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007640 , obo:ECO_0007753 .

obo:ECO_0008183 a rdfs:Class ;
  rdfs:label "protein proximity labeling assay evidence" ;
  rdfs:comment "A type of direct assay evidence in which proteins of interest are fused to an enzyme that is able to label or modify other proteins that come into the proximity of the protein of interest. Labeled/modified proteins are then isolated and identified, often by mass spectrometry." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:GO_0034763 a rdfs:Class ;
  rdfs:label "negative regulation of transmembrane transport" ;
  rdfs:subClassOf obo:GO_0034762 , obo:GO_0048523 , obo:GO_0051051 , obo:GO_0065007 .

obo:ECO_0006086 a rdfs:Class ;
  rdfs:label "Southwestern blot evidence used in manual assertion" ;
  rdfs:comment "A type of nucleic acid binding evidence that is used in a manual assertion in which DNA-protein binding is detected using labeled DNA as probes, hybridized to electrophoretically separated proteins." ;
  rdfs:subClassOf obo:ECO_0000144 , obo:ECO_0006083 .

obo:BFO_0000020 a rdfs:Class ;
  rdfs:label "specifically dependent continuant"@en ;
  rdfs:comment "A continuant that inheres in or is borne by other entities. Every instance of A requires some specific instance of B which must always be the same."@en ;
  rdfs:subClassOf obo:BFO_0000002 .

obo:ECO_0000274 a rdfs:Class ;
  rdfs:label "differential methylation hybridization evidence used in manual assertion" ;
  rdfs:comment "A type of differential methylation hybridization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000069 , obo:ECO_0001198 .

obo:ECO_0006340 a rdfs:Class ;
  rdfs:label "molecular dynamics evidence" ;
  rdfs:comment "A type of computational evidence used to study the physical movements of atoms and molecules with empirical molecular mechanics force fields." ;
  rdfs:subClassOf obo:ECO_0007672 .

obo:ECO_0007716 a rdfs:Class ;
  rdfs:label "staining evidence used in manual assertion" ;
  rdfs:comment "A type of staining evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0005019 .

obo:ECO_0008071 a rdfs:Class ;
  rdfs:label "antibody PT85-A-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody PT85-A to determine that the pig MHC class I protein complex, cattle MHC class I protein complex, and/or human MHC class I protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0008194 a rdfs:Class ;
  rdfs:label "avidity-based extracellular interaction screening evidence used in manual assertion" ;
  rdfs:comment "A type of avidity-based extracellular interaction screening evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008186 , obo:ECO_0008192 .

obo:ECO_0006261 a rdfs:Class ;
  rdfs:label "microscale thermophoresis evidence" ;
  rdfs:comment "A type of physical interaction evidence resulting from measuring the change of the fluorescent activity of a molecule as a function of binding to a non-fluorescent partner and its movement in a microspcopic thermal gradient affecting the intensity of fluorescence." ;
  rdfs:subClassOf obo:ECO_0000021 , obo:ECO_0001115 .

obo:ECO_0005559 a rdfs:Class ;
  rdfs:label "motif discovery evidence used in automatic assertion" ;
  rdfs:comment "A type of motif discovery evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005531 , obo:ECO_0005557 .

obo:ECO_0001224 a rdfs:Class ;
  rdfs:label "knockin evidence used in manual assertion" ;
  rdfs:comment "A type of knockin evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001090 , obo:ECO_0006049 .

obo:GO_0031344 a rdfs:Class ;
  rdfs:label "regulation of cell projection organization" ;
  rdfs:subClassOf obo:GO_0051128 , obo:GO_0065007 .

obo:GO_2000677 a rdfs:Class ;
  rdfs:label "regulation of transcription regulatory region DNA binding" ;
  rdfs:subClassOf obo:GO_0050789 , obo:GO_0051101 , obo:GO_0065007 .

obo:ECO_0007330 a rdfs:Class ;
  rdfs:label "phylogenetic determination of loss of key residues evidence used in automatic assertion" ;
  rdfs:comment "A type of phylogenetic determination of loss of key residues evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000216 , obo:ECO_0007826 .

obo:ECO_0000322 a rdfs:Class ;
  rdfs:label "imported manually asserted information used in automatic assertion" ;
  rdfs:comment "Evidence that was initially used in manual assertion by one person or group, which is imported by a second person or group and used in automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000313 .

obo:ECO_0007368 a rdfs:Class ;
  rdfs:label "knockin evidence used in automatic assertion" ;
  rdfs:comment "A type of knockin evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001090 , obo:ECO_0007427 .

obo:ECO_0007749 a rdfs:Class ;
  rdfs:label "transcript splice pattern evidence used in manual assertion" ;
  rdfs:comment "A type of transcript splice pattern evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000247 , obo:ECO_0000326 .

obo:OBI_0400114 a rdfs:Class ;
  rdfs:label "sonicator" ;
  rdfs:comment "A device that converts a variable electrical current to mechanical vibration of a metallic probe. The device is used for the lysis of cells, the mixing of compounds or solutions, to framgent molecules of DNA, or to create emulsions." ;
  rdfs:subClassOf obo:OBI_0001033 .

obo:ECO_0000097 a rdfs:Class ;
  rdfs:label "cDNA to DNA expression microarray evidence" ;
  rdfs:comment "A type of expression microarray evidence where expression level is quantified by fluroescently-labeled cDNA (reverse transcribed from an unknown RNA sample) hybridized to DNA immobilized on a solid surface." ;
  rdfs:subClassOf obo:ECO_0000058 .

obo:ECO_0005662 a rdfs:Class ;
  rdfs:label "regular expression motif search evidence used in manual assertion" ;
  rdfs:comment "A type of regular expression motif search evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000257 , obo:ECO_0005505 .

obo:ECO_0001010 a rdfs:Class ;
  rdfs:label "anion-exchange chromatography evidence" ;
  rdfs:comment "A type of chromatography evidence where a positively charged ion exchange resin with an affinity for molecules having net negative surface charges is used to separate the molecules." ;
  rdfs:subClassOf obo:ECO_0000325 .

obo:ECO_0005585 a rdfs:Class ;
  rdfs:label "high throughput mass spectrometry evidence" ;
  rdfs:comment "A type of mass spectrometry evidence where mass-to-charge ratio (m/z) of gas-phase ions is measured which is used to identify and quantify molecules in complex solutions within the framework of high throughput studies." ;
  rdfs:subClassOf obo:ECO_0001096 .

obo:ECO_0001205 a rdfs:Class ;
  rdfs:label "in vitro translation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro translation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001072 .

obo:ECO_0001600 a rdfs:Class ;
  rdfs:label "multilocus sequence typing evidence used in manual assertion" ;
  rdfs:comment "A type of multilocus sequence typing evidence that is used in a manual assertion" ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001598 .

obo:ECO_0006281 a rdfs:Class ;
  rdfs:label "rotary shadowing electron microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of rotary shadowing electron microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006003 , obo:ECO_0006250 .

obo:ECO_0000206 a rdfs:Class ;
  rdfs:label "BLAST evidence" ;
  rdfs:comment "A type of pairwise sequence alignment evidence obtained with basic local alignment search tool (BLAST)." ;
  rdfs:subClassOf obo:ECO_0005554 .

obo:ECO_0000311 a rdfs:Class ;
  rdfs:label "imported information" ;
  rdfs:comment "A type of documented statement evidence that is based on work performed by a person or group prior to a use by a different person or group." ;
  rdfs:subClassOf obo:ECO_0006151 .

obo:ECO_0008059 a rdfs:Class ;
  rdfs:label "antibody HB-164-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody  HB-164 to determine that the human MHC class I protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:OBI_1110030 a rdfs:Class ;
  rdfs:label "experimental infection of cell culture"@en ;
  rdfs:comment "is the administration of an infectious agent to a cell culture with the objective to have the agent colonize and replicate in culture"@en ;
  rdfs:subClassOf obo:OBI_0600000 .

obo:ECO_0000362 a rdfs:Class ;
  rdfs:label "computational inference" ;
  rdfs:comment """A type of inferential evidence based on a computationally derived unary inference or an inference chain in which at least one step is 
computationally derived.""" ;
  rdfs:subClassOf obo:ECO_0000361 , obo:ECO_0007672 .

obo:OBI_0001137 a rdfs:Class ;
  rdfs:label "protein sequencer" ;
  rdfs:comment "An device that is used to determine the order of amino acids in protein sequences." ;
  rdfs:subClassOf obo:OBI_0000832 .

obo:ECO_0008137 a rdfs:Class ;
  rdfs:label "antibody L243-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody L243-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008064 , obo:ECO_0008087 .

obo:OBI_0000985 a rdfs:Class ;
  rdfs:label "growth condition intervention design" ;
  rdfs:comment "A study design in which the independent variable is the environmental condition in which the specimen is growing" ;
  rdfs:subClassOf obo:OBI_0000115 .

obo:GO_0090343 a rdfs:Class ;
  rdfs:label "positive regulation of cell aging" ;
  rdfs:subClassOf obo:GO_0048522 , obo:GO_0051094 , obo:GO_0065007 , obo:GO_0090342 .

obo:ECO_0000234 a rdfs:Class ;
  rdfs:label "4C evidence" ;
  rdfs:comment "A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a prior circularized 3C library followed by product quantitation with a microarray." ;
  rdfs:subClassOf obo:ECO_0000232 .

obo:ECO_0001145 a rdfs:Class ;
  rdfs:label "alamarBlue assay evidence used in manual assertion" ;
  rdfs:comment "A type of alamarBlue assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001008 , obo:ECO_0007703 .

obo:GO_0019439 a rdfs:Class ;
  rdfs:label "aromatic compound catabolic process" ;
  rdfs:subClassOf obo:GO_0006725 , obo:GO_0044248 .

obo:ECO_0005592 a rdfs:Class ;
  rdfs:label "immunogold labelling electron microscopy assay evidence used in manual assertion" ;
  rdfs:comment "A type of immunogold labelling electron microscopy assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005579 , obo:ECO_0005591 , obo:ECO_0006003 .

obo:GO_2000278 a rdfs:Class ;
  rdfs:label "regulation of DNA biosynthetic process" ;
  rdfs:subClassOf obo:GO_0051052 , obo:GO_0065007 , obo:GO_2000112 .

obo:ECO_0000351 a rdfs:Class ;
  rdfs:label "partial support of intron positions by RNA-sequencing alignment evidence used in manual assertion" ;
  rdfs:comment "A type of partial support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000344 , obo:ECO_0000346 .

obo:OBI_0000447 a rdfs:Class ;
  rdfs:label "intra cellular electrophysiology recording assay" ;
  rdfs:comment "An electrophysiology recording assay where the recording location of the electrode is intracellular" ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0001047 a rdfs:Class ;
  rdfs:label "fluorescence in situ hybridization evidence" ;
  rdfs:comment "A type of nucleic acid localization evidence and in situ hybridization evidence resulting from the use of fluorescent probes to detect complementary sequences of nucleic acids." ;
  rdfs:subClassOf obo:ECO_0001534 , obo:ECO_0001836 .

obo:ECO_0007213 a rdfs:Class ;
  rdfs:label "beta-galactosidase fusion protein localization evidence used in automatic assertion" ;
  rdfs:comment "A type of beta-galactosidase fusion protein localization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000132 , obo:ECO_0007808 .

obo:GO_0035194 a rdfs:Class ;
  rdfs:label "post-transcriptional gene silencing by RNA" ;
  rdfs:subClassOf obo:GO_0016441 , obo:GO_0031047 .

obo:ECO_0007138 a rdfs:Class ;
  rdfs:label "tissue grafting phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of tissue grafting phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000243 , obo:ECO_0007634 .

obo:ECO_0006373 a rdfs:Class ;
  rdfs:label "molecular dynamics evidence used in automatic assertion" ;
  rdfs:comment "A type of molecular dynamics evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006340 , obo:ECO_0007669 .

obo:ECO_0005651 a rdfs:Class ;
  rdfs:label "matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in automatic assertion" ;
  rdfs:comment "A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005526 , obo:ECO_0007819 .

obo:GO_0042221 a rdfs:Class ;
  rdfs:label "response to chemical" ;
  rdfs:subClassOf obo:GO_0050896 .

obo:GO_0006921 a rdfs:Class ;
  rdfs:label "cellular component disassembly involved in execution phase of apoptosis" ;
  rdfs:subClassOf obo:GO_0022411 .

obo:ECO_0007486 a rdfs:Class ;
  rdfs:label "immunolocalization evidence used in automatic assertion" ;
  rdfs:comment "A type of immunolocalization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000087 , obo:ECO_0007435 , obo:ECO_0007796 .

obo:GO_1903624 a rdfs:Class ;
  rdfs:label "regulation of DNA catabolic process" ;
  rdfs:subClassOf obo:GO_0031329 , obo:GO_0051052 , obo:GO_0065007 .

obo:GO_0043086 a rdfs:Class ;
  rdfs:label "negative regulation of catalytic activity" ;
  rdfs:subClassOf obo:GO_0044092 , obo:GO_0050790 , obo:GO_0065007 .

obo:ECO_0001235 a rdfs:Class ;
  rdfs:label "MTT assay evidence used in manual assertion" ;
  rdfs:comment "A type of MTT assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001101 , obo:ECO_0007698 .

obo:ECO_0005616 a rdfs:Class ;
  rdfs:label "beta-galactosidase reporter gene assay evidence used in manual assertion" ;
  rdfs:comment "A type of beta-galactosidase reporter gene assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000270 , obo:ECO_0001802 .

obo:ECO_0008126 a rdfs:Class ;
  rdfs:label "antibody HB145-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody HB145-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008058 , obo:ECO_0008088 .

obo:ECO_0007701 a rdfs:Class ;
  rdfs:label "chemotaxis assay evidence used in manual assertion" ;
  rdfs:comment "A type of chemotaxis assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005021 , obo:ECO_0007695 .

obo:ECO_0001560 a rdfs:Class ;
  rdfs:label "single-cell RNA-sequencing evidence" ;
  rdfs:comment "A type of RNA-sequencing evidence that uses a single cell as the source of the RNA." ;
  rdfs:subClassOf obo:ECO_0000295 .

obo:ECO_0001230 a rdfs:Class ;
  rdfs:label "mass spectrometry evidence used in manual assertion" ;
  rdfs:comment "A type of mass spectrometry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001096 .

obo:GO_0071702 a rdfs:Class ;
  rdfs:label "organic substance transport" ;
  rdfs:subClassOf obo:GO_0006810 .

obo:ECO_0006333 a rdfs:Class ;
  rdfs:label "thioflavin T based fluorescence spectroscopy evidence" ;
  rdfs:comment "A type of fluorescence evidence used to assess the formation of amyloid fibrils indicated by an increase in Thioflavin T fluorescence intensity and a characteristic red shift in the emission spectrum upon binding to amyloids." ;
  rdfs:subClassOf obo:ECO_0001115 .

obo:ECO_0007260 a rdfs:Class ;
  rdfs:label "biological target-disease association via drug evidence used in automatic assertion" ;
  rdfs:comment "A type of biological target-disease association via drug that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000360 , obo:ECO_0007297 .

obo:ECO_0001844 a rdfs:Class ;
  rdfs:label "random mutagenesis of specific target DNA evidence used in manual assertion" ;
  rdfs:comment "A type of random mutagenesis of specific target DNA evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001842 , obo:ECO_0005530 .

obo:OBI_0001686 a rdfs:Class ;
  rdfs:label "in-situ hybridization assay" ;
  rdfs:comment "An assay that localizes a specific DNA or RNA sequence within a portion or section of tissue using artificially induced nucleic hybridization." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0005011 a rdfs:Class ;
  rdfs:label "ATP bioluminescence assay evidence" ;
  rdfs:comment "A type of cell viability assay evidence resulting from the analysis of ATP concentration (by measuring light intensity) when luciferase catalyzes the oxidation of luciferin in the presence of ATP, magnesium ions and molecular oxygen." ;
  rdfs:subClassOf obo:ECO_0005004 .

obo:GO_0060967 a rdfs:Class ;
  rdfs:label "negative regulation of gene silencing by RNA" ;
  rdfs:subClassOf obo:GO_0010628 , obo:GO_0048519 , obo:GO_0060966 , obo:GO_0065007 .

obo:ECO_0007665 a rdfs:Class ;
  rdfs:label "automatically integrated combinatorial computational and experimental evidence" ;
  rdfs:comment "A type of automatically integrated combinatorial evidence in which at least one line of experimental evidence and at least one line of computational evidence have been integrated automatically." ;
  rdfs:subClassOf obo:ECO_0007661 , obo:ECO_0007673 .

obo:ECO_0007231 a rdfs:Class ;
  rdfs:label "SOLiD sequencing evidence used in automatic assertion" ;
  rdfs:comment "A type of SOLiD sequencing evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000224 , obo:ECO_0007814 .

obo:ECO_0008097 a rdfs:Class ;
  rdfs:label "antibody 34-5-8S-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody 34-5-8S-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008044 , obo:ECO_0008087 .

obo:ECO_0007518 a rdfs:Class ;
  rdfs:label "northern blot evidence used in automatic assertion" ;
  rdfs:comment "A type of northern blot evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000106 , obo:ECO_0007303 .

obo:GO_0042101 a rdfs:Class ;
  rdfs:label "T cell receptor complex" ;
  rdfs:comment "A protein complex that contains a disulfide-linked heterodimer of T cell receptor (TCR) chains, which are members of the immunoglobulin superfamily, and mediates antigen recognition, ultimately resulting in T cell activation. The TCR heterodimer is associated with the CD3 complex, which consists of the nonpolymorphic polypeptides gamma, delta, epsilon, zeta, and, in some cases, eta (an RNA splice variant of zeta) or Fc epsilon chains." ;
  rdfs:subClassOf obo:OBI_1110022 .

obo:ECO_0000256 a rdfs:Class ;
  rdfs:label "match to sequence model evidence used in automatic assertion" ;
  rdfs:comment "A type of match to sequence model evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000202 , obo:ECO_0000249 .

obo:OBI_0001679 a rdfs:Class ;
  rdfs:label "yeast 2-hybrid assay" ;
  rdfs:comment "An assay that detects protein protein interactions and protein DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively. The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay." ;
  rdfs:subClassOf obo:OBI_0001146 .

obo:ECO_0007101 a rdfs:Class ;
  rdfs:label "southern hybridization evidence used in manual assertion" ;
  rdfs:comment "A type of southern hybridization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000078 , obo:ECO_0000269 .

obo:ECO_0000228 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation-qPCR evidence" ;
  rdfs:comment "A type of chromatin immunoprecipitation evidence in which protein-DNA interactions are investigated at known genomic binding sites through a process of ChIP, DNA purification, and finally quantitative polymerase chain reaction (qPCR) for DNA detection." ;
  rdfs:subClassOf obo:ECO_0000226 .

obo:OBI_0001278 a rdfs:Class ;
  rdfs:label "DNA methylation profiling by array design" ;
  rdfs:comment "A study design in which the methylation state of DNA is determined and is compared between samples using array technology." ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:ECO_0000079 a rdfs:Class ;
  rdfs:label "affinity chromatography evidence" ;
  rdfs:comment "A type of affinity evidence that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bound compound." ;
  rdfs:subClassOf obo:ECO_0000023 .

obo:ECO_0001029 a rdfs:Class ;
  rdfs:label "comet assay evidence" ;
  rdfs:comment "A type of gel electrophoresis evidence resulting in the assessment of DNA breakage in a cell, based on size and shape of DNA migration." ;
  rdfs:subClassOf obo:ECO_0000337 .

obo:ECO_0006395 a rdfs:Class ;
  rdfs:label "self-reported ancestry evidence" ;
  rdfs:comment "A type of self-reported individual's statement evidence in which a person reports their ancestry and that information is taken at face value." ;
  rdfs:subClassOf obo:ECO_0006153 .

obo:ECO_0005581 a rdfs:Class ;
  rdfs:label "enzyme-linked immunoabsorbent assay evidence used in manual assertion" ;
  rdfs:comment "A type of enzyme-linked immunoabsorbent assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000267 , obo:ECO_0000269 .

obo:ECO_0005618 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation-chip evidence used in automatic assertion" ;
  rdfs:comment "A type of chromatin immunoprecipitation-chip evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000230 , obo:ECO_0007786 .

obo:ECO_0001148 a rdfs:Class ;
  rdfs:label "annexin-V staining evidence used in manual assertion" ;
  rdfs:comment "A type of annexin-V staining evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001011 , obo:ECO_0007698 .

obo:ECO_0000333 a rdfs:Class ;
  rdfs:label "sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence" ;
  rdfs:comment "A type of gel electrophoresis evidence where proteins are separated according to their electrophoretic mobility." ;
  rdfs:subClassOf obo:ECO_0000337 .

obo:ECO_0008104 a rdfs:Class ;
  rdfs:label "antibody AG2.42.7-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody AG2.42.7-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008047 , obo:ECO_0008088 .

obo:GO_0034660 a rdfs:Class ;
  rdfs:label "ncRNA metabolic process" ;
  rdfs:subClassOf obo:GO_0016070 .

obo:ECO_0000104 a rdfs:Class ;
  rdfs:label "DNA to cDNA expression microarray evidence" ;
  rdfs:comment "A type of expression microarray evidence where expression levels are quantified by hybridization of fluorescently-labeled DNA to cDNA (reverse transcribed from an unknown RNA sample) immobilized on a solid surface." ;
  rdfs:subClassOf obo:ECO_0000058 .

obo:GO_0006820 a rdfs:Class ;
  rdfs:label "anion transport" ;
  rdfs:subClassOf obo:GO_0006811 .

obo:ECO_0007576 a rdfs:Class ;
  rdfs:label "bacterial one-hybrid evidence used in automatic assertion" ;
  rdfs:comment "A type of bacterial one-hybrid evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000294 , obo:ECO_0007432 .

obo:ECO_0001109 a rdfs:Class ;
  rdfs:label "phosphoamino acid analysis evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from the identification of the phosphorylated residue in a protein by amino acid analysis." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007180 a rdfs:Class ;
  rdfs:label "cell-detached outside-out single-channel recording evidence used in manual assertion" ;
  rdfs:comment "A type of cell-detached outside-out single-channel recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005582 , obo:ECO_0006013 .

obo:ECO_0001822 a rdfs:Class ;
  rdfs:label "survival rate analysis evidence" ;
  rdfs:comment "A type of knockout evidence based on the survival of an organism in a particular environment where a gene for an enzyme or regulator is knocked out and results are used as a natural reporter." ;
  rdfs:subClassOf obo:ECO_0001091 .

obo:ECO_0007762 a rdfs:Class ;
  rdfs:label "curator inference from Oxford Dictionary used in manual assertion" ;
  rdfs:comment "A type of curator inference from Oxford Dictionary that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007642 , obo:ECO_0007760 .

obo:GO_0032991 a rdfs:Class ;
  rdfs:label "protein-containing complex" ;
  rdfs:comment "A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together." ;
  rdfs:subClassOf obo:GO_0005575 .

obo:ECO_0001590 a rdfs:Class ;
  rdfs:label "atomic force microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of atomic force microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001589 , obo:ECO_0007435 .

obo:ECO_0001051 a rdfs:Class ;
  rdfs:label "histology evidence" ;
  rdfs:comment "A type of imaging assay evidence resulting from the qualitative microscopic examination of cells or tissues." ;
  rdfs:subClassOf obo:ECO_0000324 .

obo:ECO_0001213 a rdfs:Class ;
  rdfs:label "in vivo deubiquitination assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo deubiquitination assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001079 .

obo:ECO_0007507 a rdfs:Class ;
  rdfs:label "DNAse footprinting evidence used in automatic assertion" ;
  rdfs:comment "A type of DNAse footprinting evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001810 , obo:ECO_0007505 .

obo:ECO_0001811 a rdfs:Class ;
  rdfs:label "fluorescence anisotropy evidence" ;
  rdfs:comment "A type of fluorescence evidence based on rapid and quantitative analysis of diverse molecular interactions, enzyme activities, and nucleic acid hybridization which uses a fluorophore to measure the binding constants and kinetics of reactions that cause a change in the rotational time of the molecules." ;
  rdfs:subClassOf obo:ECO_0001115 .

obo:ECO_0006355 a rdfs:Class ;
  rdfs:label "Congo red birefringence evidence used in automatic assertion" ;
  rdfs:comment "A type of Congo red birefringence evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006332 , obo:ECO_0006353 .

obo:ECO_0006019 a rdfs:Class ;
  rdfs:label "inference based on individual clinical experience used in manual assertion" ;
  rdfs:comment "A type of inference based on individual clinical experience that is used in a manual assertion." ;
  rdfs:seeAlso <http://human-phenotype-ontology.github.io/documentation.html#annot> ;
  rdfs:subClassOf obo:ECO_0000305 , obo:ECO_0006018 .

obo:ECO_0008026 a rdfs:Class ;
  rdfs:label "coordinate mapping to geographic property shapefiles used in manual assertion" ;
  rdfs:comment "A type of coordinate mapping to geographic property shapefiles that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007631 , obo:ECO_0008014 .

obo:ECO_0006138 a rdfs:Class ;
  rdfs:label "molecular mechanics simulation evidence" ;
  rdfs:comment "A type of computational evidence where the energy of a molecular system is predicted as a function of its conformation." ;
  rdfs:subClassOf obo:ECO_0007672 .

obo:ECO_0005511 a rdfs:Class ;
  rdfs:label "duplication mutation evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence resulting from a mutation in which inserted nucleotide(s) have a sequence identical to, or derived from, adjacent nucleotides." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:GO_0120036 a rdfs:Class ;
  rdfs:label "plasma membrane bounded cell projection organization" ;
  rdfs:subClassOf obo:GO_0030030 .

obo:ECO_0000144 a rdfs:Class ;
  rdfs:label "Southwestern blot evidence" ;
  rdfs:comment "A type of nucleic acid binding evidence in which DNA-protein binding is detected using labeled DNA as probes, hybridized to electrophoretically separated proteins." ;
  rdfs:subClassOf obo:ECO_0000136 .

obo:OBI_0000049 a rdfs:Class ;
  rdfs:label "mass spectrometer" ;
  rdfs:comment "A mass spectrometer is an instrument which is used to measure the mass to charge ratio of ions. All mass spectrometers consist of three basic parts: an ion source, a mass analyzer, and a detector system. The stages within the mass spectrometer are: 1. Production of ions from the sample 2. Separation of ions with different masses 3. Detection of the number of ions of each mass produced 4.Collection of data to generate the mass spectrum" ;
  rdfs:subClassOf obo:OBI_0000832 .

obo:ECO_0007127 a rdfs:Class ;
  rdfs:label "SOLiD sequencing evidence used in manual assertion" ;
  rdfs:comment "A type of SOLiD sequencing evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000224 , obo:ECO_0000269 .

obo:ECO_0007695 a rdfs:Class ;
  rdfs:label "cell-based assay evidence used in manual assertion" ;
  rdfs:comment "A type of cell-based assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001565 .

obo:ECO_0000007 a rdfs:Class ;
  rdfs:label "immunofluorescence evidence" ;
  rdfs:comment "A type of protein detection assay evidence where a fluorescently labeled antibody is used to detect the presence or localization of a biomolecule within a cell." ;
  rdfs:subClassOf obo:ECO_0005517 .

obo:ECO_0001167 a rdfs:Class ;
  rdfs:label "conditional knockin evidence used in manual assertion" ;
  rdfs:comment "A type of conditional knockin evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001031 , obo:ECO_0001224 .

obo:ECO_0001207 a rdfs:Class ;
  rdfs:label "in vivo acetylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo acetylation assay evidence that is used in manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001074 .

obo:ECO_0001252 a rdfs:Class ;
  rdfs:label "protein sequencing assay evidence used in manual assertion" ;
  rdfs:comment "A type of protein sequencing assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001118 .

obo:ECO_0006142 a rdfs:Class ;
  rdfs:label "quantum mechanics simulation evidence used in automatic assertion" ;
  rdfs:comment "A type of quantum mechanics simulation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006141 .

obo:ECO_0007615 a rdfs:Class ;
  rdfs:label "extended field laser confocal microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of extended field laser confocal microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007057 , obo:ECO_0007490 .

obo:GO_0050920 a rdfs:Class ;
  rdfs:label "regulation of chemotaxis" ;
  rdfs:subClassOf obo:GO_0032101 , obo:GO_0040012 , obo:GO_0065007 .

obo:CHMO_0000087 a rdfs:Class ;
  rdfs:label "fluorescence microscopy assay" ;
  rdfs:comment "Any type of light microscopy assay where the specimen can be made to fluoresce (emit energy as visible light) by illuminating it with light of specific wavelengths. These specimens are called fluorophores." ;
  rdfs:subClassOf obo:CHMO_0000102 , obo:OBI_0001501 .

obo:ECO_0000170 a rdfs:Class ;
  rdfs:label "transcriptional activation assay evidence" ;
  rdfs:comment "A type of transcription assay evidence in which sequence-specific binding proteins that increase transcription of a specific target gene or genes are identified, quantified, and/or analyzed." ;
  rdfs:subClassOf obo:ECO_0000168 .

obo:ECO_0005638 a rdfs:Class ;
  rdfs:label "cell growth regulation assay evidence used in manual assertion" ;
  rdfs:comment "A type of cell growth regulation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000095 , obo:ECO_0007699 .

obo:ECO_0007738 a rdfs:Class ;
  rdfs:label "protein detection assay evidence used in manual assertion" ;
  rdfs:comment "A type of protein detection assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0005517 .

obo:GO_1903557 a rdfs:Class ;
  rdfs:label "positive regulation of tumor necrosis factor superfamily cytokine production" ;
  rdfs:subClassOf obo:GO_0001819 , obo:GO_0065007 , obo:GO_1903555 .

obo:ECO_0001589 a rdfs:Class ;
  rdfs:label "atomic force microscopy evidence" ;
  rdfs:comment "A type of microscopy evidence where a mechanical probe is applied to a sample (i.e. a type of scanning probe microscopy) which can result in production of an image (with resolution in the order of fractions of a nanometer), force measurement, or sample manipulation." ;
  rdfs:subClassOf obo:ECO_0001098 .

obo:ECO_0005522 a rdfs:Class ;
  rdfs:label "DNA dot blot assay evidence" ;
  rdfs:comment "A type of DNA detection assay evidence resulting from evaluation of the relative abundance of target sequences hybridizing to DNA samples that are immobilized on a nitrocellulose or nylon membrane." ;
  rdfs:subClassOf obo:ECO_0005519 .

obo:OBI_0100060 a rdfs:Class ;
  rdfs:label "cultured cell population" ;
  rdfs:comment "A processed material comprised of a collection of cultured cells that has been continuously maintained together in culture and shares a common propagation history." ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:ECO_0001003 a rdfs:Class ;
  rdfs:label "[3H]-thymidine incorporation assay evidence" ;
  rdfs:comment "A type of DNA synthesis cell proliferation assay evidence resulting from the measurement of cell proliferation rate by determining the incorporation of [3H]-thymidine into cellular nucleic acids." ;
  rdfs:subClassOf obo:ECO_0005008 .

obo:ECO_0008086 a rdfs:Class ;
  rdfs:label "T cell type based MHC restriction evidence" ;
  rdfs:comment "A type of direct assay evidence that employs a specific T cell type (CD4+ or CD8+) to determine that a ligand is restricted by a class of MHC molecules." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0000308 a rdfs:Class ;
  rdfs:label "biological aspect of ancestor evidence" ;
  rdfs:comment "A type of phylogenetic evidence whereby an aspect of a descendant gene is inferred through the characterization of an aspect of an ancestral gene." ;
  rdfs:subClassOf obo:ECO_0000080 .

obo:GO_0032075 a rdfs:Class ;
  rdfs:label "positive regulation of nuclease activity" ;
  rdfs:subClassOf obo:GO_0032069 , obo:GO_0045935 , obo:GO_0051345 , obo:GO_0065007 .

obo:OBI_0000415 a rdfs:Class ;
  rdfs:label "polymerase chain reaction"@en ;
  rdfs:comment "PCR is the process in which a DNA polymerase is used to amplify a piece of DNA by in vitro enzymatic replication. As PCR progresses, the DNA thus generated is itself used as a template for replication. This sets in motion a chain reaction in which the DNA template is exponentially amplified."@en ;
  rdfs:subClassOf obo:OBI_0600058 .

obo:ECO_0008008 a rdfs:Class ;
  rdfs:label "rule-based machine learning method evidence used in automatic assertion" ;
  rdfs:comment "A type of rule-based machine learning method evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008002 , obo:ECO_0008004 .

obo:GO_0019438 a rdfs:Class ;
  rdfs:label "aromatic compound biosynthetic process" ;
  rdfs:subClassOf obo:GO_0006725 , obo:GO_0044249 .

obo:ECO_0001218 a rdfs:Class ;
  rdfs:label "in vivo protein kinase assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo protein kinase assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001085 , obo:ECO_0005801 .

obo:ECO_0001829 a rdfs:Class ;
  rdfs:label "ferric uptake regulator titration assay evidence" ;
  rdfs:comment "A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis." ;
  rdfs:subClassOf obo:ECO_0000049 .

obo:ECO_0007251 a rdfs:Class ;
  rdfs:label "competitive growth assay evidence used in automatic assertion" ;
  rdfs:comment "A type of competitive growth assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000336 , obo:ECO_0007775 .

obo:ECO_0007156 a rdfs:Class ;
  rdfs:label "biological target-disease association via drug evidence used in manual assertion" ;
  rdfs:comment "A type of biological target-disease association via drug that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0000360 .

obo:ECO_0006238 a rdfs:Class ;
  rdfs:label "galactokinase reporter gene assay evidence" ;
  rdfs:comment "A type of reporter gene assay evidence based on the fusion of the galK gene to a specific promoter for the expression of the enzyme galactokinase." ;
  rdfs:subClassOf obo:ECO_0000049 .

obo:ECO_0006008 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation- exonuclease evidence used in manual assertion" ;
  rdfs:comment "A type of chromatin immunoprecipitation- exonuclease evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001806 , obo:ECO_0005644 .

obo:ECO_0007531 a rdfs:Class ;
  rdfs:label "yeast 2-hybrid evidence used in automatic assertion" ;
  rdfs:comment "A type of yeast 2-hybrid evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000068 , obo:ECO_0007432 .

obo:ECO_0000064 a rdfs:Class ;
  rdfs:label "functional complementation in heterologous system evidence" ;
  rdfs:comment "A type of functional complementation evidence that is based on the insertion of a wild-type copy of a gene into a heterologous organism, with the mutation occurring in a homologous gene." ;
  rdfs:subClassOf obo:ECO_0000012 .

obo:ECO_0007332 a rdfs:Class ;
  rdfs:label "physical interaction evidence used in automatic assertion" ;
  rdfs:comment "A type of physical interaction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000021 , obo:ECO_0007307 .

obo:ECO_0007727 a rdfs:Class ;
  rdfs:label "nucleic acid localization evidence used in manual assertion" ;
  rdfs:comment "A type of nucleic acid localization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001534 .

obo:ECO_0000318 a rdfs:Class ;
  rdfs:label "biological aspect of ancestor evidence used in manual assertion" ;
  rdfs:comment "A type of biological aspect of ancestor evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/iba-inferred-biological-aspect-ancestor> ;
  rdfs:subClassOf obo:ECO_0000252 , obo:ECO_0000308 .

obo:GO_0010638 a rdfs:Class ;
  rdfs:label "positive regulation of organelle organization" ;
  rdfs:subClassOf obo:GO_0033043 , obo:GO_0051130 , obo:GO_0065007 .

obo:GO_1901361 a rdfs:Class ;
  rdfs:label "organic cyclic compound catabolic process" ;
  rdfs:subClassOf obo:GO_1901360 , obo:GO_1901575 .

obo:ECO_0007052 a rdfs:Class ;
  rdfs:label "transmitted light brightfied mircoscopy evidence used in manual assertion" ;
  rdfs:comment "A type of transmitted light brightfied mircoscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001232 , obo:ECO_0007051 .

obo:ECO_0007684 a rdfs:Class ;
  rdfs:label "protein separation evidence used in manual assertion" ;
  rdfs:comment "A type of protein separation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000156 , obo:ECO_0006005 .

obo:GO_0071704 a rdfs:Class ;
  rdfs:label "organic substance metabolic process" ;
  rdfs:subClassOf obo:GO_0008152 .

obo:GO_0008219 a rdfs:Class ;
  rdfs:label "cell death" ;
  rdfs:subClassOf obo:GO_0009987 .

obo:GO_0060326 a rdfs:Class ;
  rdfs:label "cell chemotaxis" ;
  rdfs:subClassOf obo:GO_0006935 , obo:GO_0016477 .

obo:ECO_0008003 a rdfs:Class ;
  rdfs:label "machine learning method evidence used in manual assertion" ;
  rdfs:comment "A type of machine learning method evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007668 , obo:ECO_0008000 .

obo:ECO_0005649 a rdfs:Class ;
  rdfs:label "machine learning prediction of motif instance evidence used in manual assertion" ;
  rdfs:comment "A type of machine learning prediction of motif instance evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000255 , obo:ECO_0005535 .

obo:GO_0009058 a rdfs:Class ;
  rdfs:label "biosynthetic process" ;
  rdfs:subClassOf obo:GO_0008152 .

obo:ECO_0008037 a rdfs:Class ;
  rdfs:label "curator inference based on intrinsic disorder prediction used in manual assertion" ;
  rdfs:comment "A type of curator inference based on intrinsic disorder prediction that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000244 , obo:ECO_0000305 , obo:ECO_0008032 .

obo:ECO_0000261 a rdfs:Class ;
  rdfs:label "targeting sequence prediction evidence used in automatic assertion" ;
  rdfs:comment "A type of targeting sequence prediction that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000081 , obo:ECO_0000258 .

obo:GO_2000146 a rdfs:Class ;
  rdfs:label "negative regulation of cell motility" ;
  rdfs:subClassOf obo:GO_0040013 , obo:GO_0051271 , obo:GO_0065007 , obo:GO_2000145 .

obo:GO_1905954 a rdfs:Class ;
  rdfs:label "positive regulation of lipid localization" ;
  rdfs:subClassOf obo:GO_0048518 , obo:GO_0065007 , obo:GO_1905952 .

obo:ECO_0008014 a rdfs:Class ;
  rdfs:label "coordinate mapping to geographic property shapefiles" ;
  rdfs:comment "A type of computational inference where a geographic property of an entity is determined by searching coordinates associated with the entity against polygons with associated geographic properties in the form of shapefiles." ;
  rdfs:subClassOf obo:ECO_0000362 .

obo:GO_0002832 a rdfs:Class ;
  rdfs:label "negative regulation of response to biotic stimulus" ;
  rdfs:subClassOf obo:GO_0002831 , obo:GO_0048585 , obo:GO_0065007 .

obo:ECO_0005554 a rdfs:Class ;
  rdfs:label "pairwise sequence alignment evidence" ;
  rdfs:comment "A type of sequence alignment evidence where two sequences are aligned to identify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences (protein or nucleic acid)." ;
  rdfs:subClassOf obo:ECO_0000200 .

obo:ECO_0001022 a rdfs:Class ;
  rdfs:label "carboxyfluorescein diacetate succinimidyl ester staining evidence" ;
  rdfs:comment "A type of staining evidence resulting from monitoring division by the use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to label intracellular molecules with carboxyfluorescein; when a labeled cell divides, the number of carboxyfluorescein-tagged molecules is split." ;
  rdfs:subClassOf obo:ECO_0005019 .

obo:ECO_0007471 a rdfs:Class ;
  rdfs:label "resonant mirror biosensor evidence used in automatic assertion" ;
  rdfs:comment "A type of resonant mirror biosensor evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001123 , obo:ECO_0007307 .

obo:ECO_0005600 a rdfs:Class ;
  rdfs:label "immunofluorescence confocal microscopy evidence" ;
  rdfs:comment "A type of confocal microscopy evidence where light passes through immunofluorescent-stained tissue sections or cells and then the emitted light passes through the dichroic mirror and is focused onto the pinhole for subsequent detection." ;
  rdfs:subClassOf obo:ECO_0000007 , obo:ECO_0005587 .

obo:GO_0031327 a rdfs:Class ;
  rdfs:label "negative regulation of cellular biosynthetic process" ;
  rdfs:subClassOf obo:GO_0009890 , obo:GO_0031324 , obo:GO_0031326 , obo:GO_0065007 .

obo:ECO_0000239 a rdfs:Class ;
  rdfs:label "3C-seq evidence" ;
  rdfs:comment "A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with multiplexed high-throughput (HT) sequencing." ;
  rdfs:subClassOf obo:ECO_0000232 .

obo:ECO_0007431 a rdfs:Class ;
  rdfs:label "mammalian 2-hybrid assay evidence used in automatic assertion" ;
  rdfs:comment "A type of mammalian 2-hybrid assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001095 , obo:ECO_0007432 .

obo:ECO_0007318 a rdfs:Class ;
  rdfs:label "subtractive hybridization evidence used in automatic assertion" ;
  rdfs:comment "A type of subtractive hybridization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000118 , obo:ECO_0007303 .

obo:ECO_0007085 a rdfs:Class ;
  rdfs:label "Etest evidence" ;
  rdfs:comment "A type of zone of inhibition evidence resulting from testing the antimicrobial susceptibility by using predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip." ;
  rdfs:subClassOf obo:ECO_0007083 .

obo:ECO_0000037 a rdfs:Class ;
  rdfs:label "obsolete not_recorded" ;
  rdfs:comment "The evidence not_recorded appears in some legacy annotations; it should not be used for new annotations." .

obo:ECO_0007390 a rdfs:Class ;
  rdfs:label "immunocytochemistry evidence used in automatic assertion" ;
  rdfs:comment "A type of immunocytochemistry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001053 , obo:ECO_0007819 .

obo:GO_0009636 a rdfs:Class ;
  rdfs:label "response to toxic substance" ;
  rdfs:subClassOf obo:GO_0042221 .

obo:OBI_0002438 a rdfs:Class ;
  rdfs:label "nuclear ligation assay" ;
  rdfs:comment "An assay that detects the proximity of chromosomal DNA through the use of a ligation reaction in isolated nuclei." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:OBI_0002075 a rdfs:Class ;
  rdfs:label "MHC ligand assay" ;
  rdfs:comment "An immune epitope assay that characterizes the structrue of a MHC-ligand complex, or detects the processing and presentation of a ligand by an antigen presenting cell, or the binding of a ligand to an MHC molecule." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_1110128 .

obo:OBI_0000903 a rdfs:Class ;
  rdfs:label "in vitro cell killing assay" ;
  rdfs:comment "A cell killing assay that measures if and how many target cells are actively killed by other cells in a cell culture." ;
  rdfs:subClassOf obo:OBI_0000882 .

obo:ECO_0007369 a rdfs:Class ;
  rdfs:label "conditional knockout evidence used in automatic assertion" ;
  rdfs:comment "A type of conditional knockout evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001030 , obo:ECO_0007370 .

obo:OBI_0400103 a rdfs:Class ;
  rdfs:label "DNA sequencer" ;
  rdfs:comment "A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences." ;
  rdfs:subClassOf obo:OBI_0000832 .

obo:PATO_0001296 a rdfs:Class ;
  rdfs:label "luminous flux" ;
  rdfs:comment "A scalar optical quality which obtains by the magnitude of the light emitted by the bearer." ;
  rdfs:subClassOf obo:PATO_0001300 .

obo:ECO_0007343 a rdfs:Class ;
  rdfs:label "allograft transplantation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of allograft transplantation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001009 , obo:ECO_0007810 .

obo:OBI_0000444 a rdfs:Class ;
  rdfs:label "target of material addition role"@en ;
  rdfs:comment "target of material addition role is a role realized by an entity into which a material is added in a material addition process" ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:ECO_0007470 a rdfs:Class ;
  rdfs:label "restriction landmark genomic scanning evidence used in automatic assertion" ;
  rdfs:comment "A type of restriction landmark genomic scanning evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000089 , obo:ECO_0007817 .

obo:OBI_0000038 a rdfs:Class ;
  rdfs:label "chromatography column" ;
  rdfs:comment "Chromatography column in chemistry is a tube and contents (typically glass) used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms." ;
  rdfs:subClassOf obo:OBI_0000932 .

obo:GO_0043068 a rdfs:Class ;
  rdfs:label "positive regulation of programmed cell death" ;
  rdfs:subClassOf obo:GO_0010942 , obo:GO_0043067 , obo:GO_0065007 .

obo:ECO_0001202 a rdfs:Class ;
  rdfs:label "in vitro protein kinase assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro protein kinase assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001068 , obo:ECO_0005801 .

obo:ECO_0006344 a rdfs:Class ;
  rdfs:label "sporulation assay evidence" ;
  rdfs:comment "A type of direct assay evidence that reflects the presence, absence, and/or amount of spore formation." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0008075 a rdfs:Class ;
  rdfs:label "antibody W6/32-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody W6/32 to determine that the MHC class I protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0000279 a rdfs:Class ;
  rdfs:label "qualitative western immunoblotting evidence used in manual assertion" ;
  rdfs:comment "A type of qualitative western immunoblotting evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000112 , obo:ECO_0000284 , obo:ECO_0000314 .

obo:ECO_0001123 a rdfs:Class ;
  rdfs:label "resonant mirror biosensor evidence" ;
  rdfs:comment "A type of direct assay evidence from the analysis of the real-time interaction between an analyte in solution and its interactant linked to the surface of the resonant mirror." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:GO_0015075 a rdfs:Class ;
  rdfs:label "ion transmembrane transporter activity" ;
  rdfs:subClassOf obo:GO_0022857 .

obo:ECO_0005623 a rdfs:Class ;
  rdfs:label "comparative genomics motif search evidence used in automatic assertion" ;
  rdfs:comment "A type of comparative genomics motif search evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000258 , obo:ECO_0005534 .

obo:OBI_0001181 a rdfs:Class ;
  rdfs:label "binding datum" ;
  rdfs:comment "A data item that states if two or more material entities have the disposition to form a complex, and if so, how strong that disposition is. " ;
  rdfs:subClassOf obo:IAO_0000027 .

obo:OBI_0001738 a rdfs:Class ;
  rdfs:label "X-ray crystallography assay determining the 3D structure of a B cell epitope:antibody complex" ;
  rdfs:comment "A X-ray crystallography 3D molecular structure determination assay that characterizes the 3-dimensional molecular structrue of a B cell epitope:antibody complex." ;
  rdfs:subClassOf obo:OBI_0000912 , obo:OBI_0001721 .

obo:ECO_0007074 a rdfs:Class ;
  rdfs:label "bacterial 2-hybrid assay evidence used in manual assertion" ;
  rdfs:comment "A type of bacterial 2-hybrid assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006077 , obo:ECO_0007073 .

obo:IAO_0000003 a rdfs:Class ;
  rdfs:label "measurement unit label"@en ;
  rdfs:comment "A measurement unit label is as a label that is part of a scalar measurement datum and denotes a unit of measure."@en ;
  rdfs:subClassOf obo:IAO_0000009 .

obo:ECO_0006069 a rdfs:Class ;
  rdfs:label "RNA-sequencing evidence used in automatic assertion" ;
  rdfs:comment "A type of high throughput nucleotide sequencing assay evidence used in an automatic assertion based on high-throughput (HT) sequencing of fragmented cDNA molecules." ;
  rdfs:subClassOf obo:ECO_0000295 , obo:ECO_0007303 .

obo:ECO_0007080 a rdfs:Class ;
  rdfs:label "colony color phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of colony color phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007078 , obo:ECO_0007079 .

obo:ECO_0005504 a rdfs:Class ;
  rdfs:label "spectrometry evidence" ;
  rdfs:comment "A type of experimental evidence resulting from the use of a spectrometer." ;
  rdfs:subClassOf obo:ECO_0000006 .

obo:ECO_0001241 a rdfs:Class ;
  rdfs:label "nucleotide analog incorporation assay evidence used in manual assertion" ;
  rdfs:comment "A type of nucleotide analog incorporation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001107 , obo:ECO_0007697 .

obo:ECO_0000126 a rdfs:Class ;
  rdfs:label "green fluorescent protein fusion protein localization evidence" ;
  rdfs:comment "A type of fusion protein localization evidence resulting from the labeling of a protein of interest with green fluorescent protein (GFP) from the jellyfish Aequorea victoria." ;
  rdfs:subClassOf obo:ECO_0000124 .

obo:OBI_0001591 a rdfs:Class ;
  rdfs:label "direct binding assay" ;
  rdfs:comment "A binding assay that measures the formation or disassociation of a complex of 2 material entities directly without use of a competitve ligand." ;
  rdfs:subClassOf obo:OBI_0001146 .

obo:ECO_0007587 a rdfs:Class ;
  rdfs:label "high throughput mutant phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of high throughput mutant phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006057 , obo:ECO_0007000 , obo:ECO_0007325 .

obo:ECO_0006153 a rdfs:Class ;
  rdfs:label "self-reported individual's statement evidence" ;
  rdfs:comment "A type of documented statement evidence in which information is provided by an individual through means including, but not limited to, paper form, electronic form, or verbal communication, and is captured in a documented record." ;
  rdfs:subClassOf obo:ECO_0006151 .

obo:GO_0016441 a rdfs:Class ;
  rdfs:label "posttranscriptional gene silencing" ;
  rdfs:subClassOf obo:GO_0010608 , obo:GO_0010629 .

obo:GO_2001251 a rdfs:Class ;
  rdfs:label "negative regulation of chromosome organization" ;
  rdfs:subClassOf obo:GO_0010639 , obo:GO_0033044 , obo:GO_0065007 .

obo:OBI_1110037 a rdfs:Class ;
  rdfs:label "assay measuring binding of a T cell epitope:MHC:TCR complex" ;
  rdfs:comment "A T cell epitope assay that measures the binding of T cell receptor to an epitope." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_0003128 .

obo:ECO_0005574 a rdfs:Class ;
  rdfs:label "cell-detached inside-out single-channel recording evidence" ;
  rdfs:comment "A type of patch-clamp recording evidence that involves study of individual ion channels by gently pulling the patch of membrane away from the cell, and the patch remains attached to the pipette with its cytoplasmic surface exposed to the bathing solution." ;
  rdfs:subClassOf obo:ECO_0006012 .

obo:GO_0034767 a rdfs:Class ;
  rdfs:label "positive regulation of ion transmembrane transport" ;
  rdfs:subClassOf obo:GO_0034764 , obo:GO_0034765 , obo:GO_0043270 , obo:GO_0065007 .

obo:ECO_0007090 a rdfs:Class ;
  rdfs:label "structural similarity evidence used in manual assertion" ;
  rdfs:comment "A type of structural similarity evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000027 , obo:ECO_0000252 .

obo:IAO_0000109 a rdfs:Class ;
  rdfs:label "measurement datum"@en ;
  rdfs:comment "A measurement datum is an information content entity that is a recording of the output of a measurement such as produced by a device."@en ;
  rdfs:subClassOf obo:IAO_0000027 .

obo:ECO_0001014 a rdfs:Class ;
  rdfs:label "blocking peptide evidence" ;
  rdfs:comment "A type of immunological assay evidence resulting from the use of peptides to block antibodies from binding to their targets." ;
  rdfs:subClassOf obo:ECO_0000040 .

obo:OBI_0001318 a rdfs:Class ;
  rdfs:label "proteomic profiling by array assay" ;
  rdfs:comment "An assay that detects proteins in a sample (quantified or otherwise analyzed), e.g. antibody profiling using an array based technology." ;
  rdfs:subClassOf obo:OBI_0001985 , obo:OBI_2100023 .

obo:ECO_0005666 a rdfs:Class ;
  rdfs:label "S1 nuclease protection assay evidence used in manual assertion" ;
  rdfs:comment "A type of S1 nuclease protection assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001240 , obo:ECO_0005521 .

obo:ECO_0000267 a rdfs:Class ;
  rdfs:label "enzyme-linked immunoabsorbent assay evidence" ;
  rdfs:comment "A type of protein detection assay evidence where an antigen is bound to a substrate, and an antibody directly or indirectly linked to an enzyme is utilized to determine the amount of bound antigen which is visualized with a color change in the substrate." ;
  rdfs:subClassOf obo:ECO_0005517 .

obo:ECO_0006384 a rdfs:Class ;
  rdfs:label "host-surrogate penetration assay evidence used in manual assertion" ;
  rdfs:comment "A type of host-surrogate penetration assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0006347 .

obo:GO_0060491 a rdfs:Class ;
  rdfs:label "regulation of cell projection assembly" ;
  rdfs:subClassOf obo:GO_0031344 , obo:GO_0044087 , obo:GO_0065007 .

obo:ECO_0001239 a rdfs:Class ;
  rdfs:label "nuclear fragmentation evidence used in manual assertion" ;
  rdfs:comment "A type of nuclear fragmentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001104 , obo:ECO_0007698 .

obo:GO_0034613 a rdfs:Class ;
  rdfs:label "cellular protein localization" ;
  rdfs:subClassOf obo:GO_0008104 , obo:GO_0070727 .

obo:ECO_0007245 a rdfs:Class ;
  rdfs:label "cytochalasin experiment evidence used in automatic assertion" ;
  rdfs:comment "A type of cytochalasin experiment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000299 , obo:ECO_0007811 .

obo:OBI_0000874 a rdfs:Class ;
  rdfs:label "detection of specific nucleic acid polymers with complementary probes" ;
  rdfs:comment "An analyte assay in which a specified input material (the evaluant) is examined for the presence or quantity of specified nucleic acid polymers, which are identified based on the use of complementary nucleic acid probes." ;
  rdfs:subClassOf obo:OBI_0000443 .

obo:ECO_0007578 a rdfs:Class ;
  rdfs:label "functional complementation evidence used in automatic assertion" ;
  rdfs:comment "A type of functional complementation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000012 , obo:ECO_0007326 .

obo:ECO_0007678 a rdfs:Class ;
  rdfs:label "combinatorial computational evidence used in manual assertion" ;
  rdfs:comment "A type of combinatorial computational evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000244 , obo:ECO_0007677 .

obo:ECO_0006329 a rdfs:Class ;
  rdfs:label "static fluorescence quenching evidence used in manual assertion" ;
  rdfs:comment "A type of static fluorescence quenching evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000353 , obo:ECO_0001249 , obo:ECO_0006274 .

obo:ECO_0001840 a rdfs:Class ;
  rdfs:label "colorimetric in situ hybridization evidence used in automatic assertion" ;
  rdfs:comment "A type of colorimetric in situ hybridization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001837 , obo:ECO_0001839 .

obo:ECO_0007773 a rdfs:Class ;
  rdfs:label "cell-based assay evidence used in automatic assertion" ;
  rdfs:comment "A type of cell-based assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001565 , obo:ECO_0007307 .

obo:GO_0071840 a rdfs:Class ;
  rdfs:label "cellular component organization or biogenesis" ;
  rdfs:subClassOf obo:GO_0009987 .

obo:ECO_0001019 a rdfs:Class ;
  rdfs:label "caspase assay evidence" ;
  rdfs:comment "A type of apoptotic assay evidence resulting from the detection of caspase activation, which results in the cleaving of intracellular substrates during apoptosis." ;
  rdfs:subClassOf obo:ECO_0005034 .

obo:ECO_0001033 a rdfs:Class ;
  rdfs:label "cross-linking evidence" ;
  rdfs:comment "A type of protein binding evidence resulting from the identification of two interacting proteins (that exist in close proximity) by linking through covalent bonds followed by identification" ;
  rdfs:subClassOf obo:ECO_0000024 .

obo:UBERON_0000463 a rdfs:Class ;
  rdfs:label "organism substance" ;
  rdfs:comment "Material anatomical entity in a gaseous, liquid, semisolid or solid state; produced by anatomical structures or derived from inhaled and ingested substances that have been modified by anatomical structures as they pass through the body." ;
  rdfs:subClassOf obo:UBERON_0000465 .

obo:ECO_0000329 a rdfs:Class ;
  rdfs:label "whole transcript splice pattern evidence used in manual assertion" ;
  rdfs:comment "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in manual assertion." ;
  rdfs:subClassOf obo:ECO_0000247 , obo:ECO_0000327 .

obo:ECO_0007242 a rdfs:Class ;
  rdfs:label "tissue grafting phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of tissue grafting phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000243 , obo:ECO_0007810 .

obo:ECO_0007673 a rdfs:Class ;
  rdfs:label "automatically integrated combinatorial evidence" ;
  rdfs:comment "A type of combinatorial evidence in which at least two distinct types of evidence have been integrated automatically." ;
  rdfs:subClassOf obo:ECO_0000212 .

obo:ECO_0005571 a rdfs:Class ;
  rdfs:label "current clamp recording evidence" ;
  rdfs:comment "A type of electrophysiology assay evidence that involves recording the change in membrane potential caused by applying specific current to the cell." ;
  rdfs:subClassOf obo:ECO_0000164 .

obo:ECO_0005650 a rdfs:Class ;
  rdfs:label "machine learning prediction of motif instance evidence used in automatic assertion" ;
  rdfs:comment "A type of machine learning prediction of motif instance evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000256 , obo:ECO_0005535 .

obo:ECO_0006271 a rdfs:Class ;
  rdfs:label "fluorescence microscopy evidence" ;
  rdfs:comment "A type of microscopy evidence that is obtained by using the fluorescence of the studied sample instead of or in addition to the scattering, reflection, attenuation or absorption of light." ;
  rdfs:subClassOf obo:ECO_0001098 , obo:ECO_0001115 .

obo:ECO_0005612 a rdfs:Class ;
  rdfs:label "obsolete inference from phenotype manipulation evidence" ;
  rdfs:comment "OBSOLETE. A type of mutant phenotype evidence used in manual assertion where variations or changes, such as mutations or abnormal levels of the product(s) of a single gene of interest, including non-mutational changes such as inhibition with antibodies or other inhibitors are determined." .

obo:ECO_0007359 a rdfs:Class ;
  rdfs:label "chemiluminescence-linked immunoassay evidence used in automatic assertion" ;
  rdfs:comment "A type of chemiluminescence-linked immunoassay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001023 , obo:ECO_0007819 .

obo:GO_0040033 a rdfs:Class ;
  rdfs:label "RNA-mediated gene silencing by inhibition of translation" ;
  rdfs:subClassOf obo:GO_0017148 , obo:GO_0035194 .

obo:ECO_0007279 a rdfs:Class ;
  rdfs:label "whole-cell voltage clamp recording evidence used in automatic assertion" ;
  rdfs:comment "A type of whole-cell voltage clamp recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005572 , obo:ECO_0007766 .

obo:GO_0005622 a rdfs:Class ;
  rdfs:label "intracellular anatomical structure" ;
  rdfs:subClassOf obo:GO_0110165 .

obo:ECO_0001228 a rdfs:Class ;
  rdfs:label "macroscopy evidence used in manual assertion" ;
  rdfs:comment "A type of macroscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001094 .

obo:ECO_0007040 a rdfs:Class ;
  rdfs:label "plaque assay evidence used in manual assertion" ;
  rdfs:comment "A type of plaque assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007039 , obo:ECO_0007699 .

obo:ECO_0006250 a rdfs:Class ;
  rdfs:label "rotary shadowing electron microscopy evidence" ;
  rdfs:comment "A type of electron microscopy evidence where a heavy metal is evaporated onto surface adsorbed molecules and macromolecular assemblies allowing high-contrast visualization of both individual macromolecules and the surface structure of macromolecular assemblies." ;
  rdfs:subClassOf obo:ECO_0005033 .

obo:ECO_0007482 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence based on homology evidence used in automatic assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence based on homology evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005549 , obo:ECO_0007827 .

obo:ECO_0006304 a rdfs:Class ;
  rdfs:label "microscale thermophoresis evidence used in automatic assertion" ;
  rdfs:comment "A type of microscale thermophoresis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006261 , obo:ECO_0007332 , obo:ECO_0007450 .

obo:ECO_0007654 a rdfs:Class ;
  rdfs:label "combinatorial experimental evidence" ;
  rdfs:comment "A type of combinatorial evidence in which two or more distinct types of experimental evidence are integrated." ;
  rdfs:subClassOf obo:ECO_0000212 .

obo:ECO_0007039 a rdfs:Class ;
  rdfs:label "plaque assay evidence" ;
  rdfs:comment "A type of cell growth assay evidence resulting in direct quantification of infectious virons and antiviral substances through the counting of discrete plaques (infectious units and cellular dead zones) in cell culture." ;
  rdfs:subClassOf obo:ECO_0001563 .

obo:ECO_0006050 a rdfs:Class ;
  rdfs:label "anti-sense experiment evidence used in manual assertion" ;
  rdfs:comment "A type of anti-sense experiment evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000018 , obo:ECO_0000315 .

obo:ECO_0007442 a rdfs:Class ;
  rdfs:label "nuclear fragmentation evidence used in automatic assertion" ;
  rdfs:comment "A type of nuclear fragmentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001104 , obo:ECO_0007777 .

obo:ECO_0001191 a rdfs:Class ;
  rdfs:label "in vitro protein cleavage assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro protein cleavage assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001058 .

obo:CHEBI_23528 a rdfs:Class ;
  rdfs:label "cytochalasin" ;
  rdfs:subClassOf obo:CHEBI_23367 .

obo:ECO_0000029 a rdfs:Class ;
  rdfs:label "match to InterPro member signature evidence" ;
  rdfs:comment "A type of match to sequence model evidence resulting from a positive match of a protein, or set of proteins to a predictive model (signature) in the InterPro database." ;
  rdfs:subClassOf obo:ECO_0000202 .

obo:ECO_0007784 a rdfs:Class ;
  rdfs:label "obsolete protein assay evidence used in automatic assertion" ;
  rdfs:comment "OBSOLETE. A type of protein assay evidence that is used in an automatic assertion." .

obo:ECO_0001008 a rdfs:Class ;
  rdfs:label "alamarBlue assay evidence" ;
  rdfs:comment "A type of cell viability assay evidence resulting from the continuous measurement of fluorescence intensity from the viable cells, where a cell permeable compound, resazurin (the active ingredient of alamarBlue), is reduced to fluorescent resorufin." ;
  rdfs:subClassOf obo:ECO_0005004 .

obo:GO_0031323 a rdfs:Class ;
  rdfs:label "regulation of cellular metabolic process" ;
  rdfs:subClassOf obo:GO_0019222 , obo:GO_0050794 , obo:GO_0065007 .

obo:ECO_0001152 a rdfs:Class ;
  rdfs:label "blocking polyclonal antibody evidence used in manual assertion" ;
  rdfs:comment "A type of blocking polyclonal antibody evidence tthat is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001015 , obo:ECO_0006078 .

obo:ECO_0007206 a rdfs:Class ;
  rdfs:label "intermethylated site amplification evidence used in automatic assertion" ;
  rdfs:comment "A type of intermethylated site amplification evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000086 , obo:ECO_0007401 .

obo:ECO_0006210 a rdfs:Class ;
  rdfs:label "small-angle X-ray scattering evidence used in manual assertion" ;
  rdfs:comment "A type of small-angle X-ray scattering evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006182 , obo:ECO_0007062 .

obo:PR_000023089 a rdfs:Class ;
  rdfs:label "DNA ligase" ;
  rdfs:comment "A protein that is a translation product of the Escherichia coli K-12 ligA gene or a 1:1 ortholog thereof." ;
  rdfs:subClassOf obo:OBI_0000427 , obo:PR_000000001 .

obo:ECO_0007594 a rdfs:Class ;
  rdfs:label "voltammetry evidence used in automatic assertion" ;
  rdfs:comment "A type of voltammetry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007015 , obo:ECO_0007805 .

obo:ECO_0007100 a rdfs:Class ;
  rdfs:label "methylation-specific polymerase chain reaction evidence used in manual assertion" ;
  rdfs:comment "A type of methylation-specific polymerase chain reaction evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000077 , obo:ECO_0001198 .

obo:ECO_0007314 a rdfs:Class ;
  rdfs:label "differential hybridization evidence used in automatic assertion" ;
  rdfs:comment "A type of differential hybridization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000116 , obo:ECO_0007303 .

obo:ECO_0007719 a rdfs:Class ;
  rdfs:label "immunodetection assay evidence used in manual assertion" ;
  rdfs:comment "A type of immunodetection assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0005593 .

obo:ECO_0007202 a rdfs:Class ;
  rdfs:label "morphological similarity evidence used in automatic assertion" ;
  rdfs:comment "A type of morphological similarity evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000071 , obo:ECO_0007824 .

obo:ECO_0000156 a rdfs:Class ;
  rdfs:label "protein separation evidence" ;
  rdfs:comment "A type of fractionation evidence resulting from the isolation of a single type of protein from a complex mixture." ;
  rdfs:subClassOf obo:ECO_0000100 .

obo:ECO_0006260 a rdfs:Class ;
  rdfs:label "protein thermal shift assay evidence" ;
  rdfs:comment "A type of temperature-induced protein unfolding evidence that assess thermal denaturation temperature and protein stability under different experimental conditions, e.g. buffer pH or ionic strength, redox potential, usually by means of intrinsic fluorescence or thermofluor." ;
  rdfs:subClassOf obo:ECO_0006268 .

obo:OBI_0000806 a rdfs:Class ;
  rdfs:label "material maintenance objective" ;
  rdfs:comment "An objective specification maintains some or all of the qualities of a material over time." ;
  rdfs:subClassOf obo:IAO_0000005 .

obo:ECO_0005639 a rdfs:Class ;
  rdfs:label "cell growth regulation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of cell growth regulation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000095 , obo:ECO_0007778 .

obo:OBI_0001239 a rdfs:Class ;
  rdfs:label "transcription profiling by high throughput sequencing design" ;
  rdfs:comment "A study design in which sequencing technology (e.g. Solexa/454) is used to generate RNA sequence, analyse the transcibed regions of the genome, and/or to quantitate transcript abundance" ;
  rdfs:subClassOf obo:OBI_0500000 .

obo:ECO_0007167 a rdfs:Class ;
  rdfs:label "splice site mutation phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of splice site mutation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0005514 .

obo:ECO_0007661 a rdfs:Class ;
  rdfs:label "combinatorial computational and experimental evidence" ;
  rdfs:comment "A type of combinatorial evidence in which at least one line of experimental evidence and at least one line of computational evidence have been integrated." ;
  rdfs:subClassOf obo:ECO_0000212 .

obo:ECO_0006318 a rdfs:Class ;
  rdfs:label "temperature-induced protein unfolding evidence used in automatic assertion" ;
  rdfs:comment "A type of temperature-induced protein unfolding evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006268 , obo:ECO_0006312 .

obo:ECO_0005021 a rdfs:Class ;
  rdfs:label "chemotaxis assay evidence" ;
  rdfs:comment "A type of cell-based assay evidence resulting from the evaluation of chemotactic ability (i.e. the movement, or lack of movement, in response to a chemical stimulus) of prokaryotic or eukaryotic cells." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:GO_0032259 a rdfs:Class ;
  rdfs:label "methylation" ;
  rdfs:subClassOf obo:GO_0008152 .

obo:ECO_0000290 a rdfs:Class ;
  rdfs:label "subtractive hybridization evidence used in manual assertion" ;
  rdfs:comment "A type of subtractive hybridization experiment that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000118 , obo:ECO_0000291 .

obo:ECO_0006257 a rdfs:Class ;
  rdfs:label "beta galactosidase functional complementation evidence" ;
  rdfs:comment "A type of protein fragment functional complementaion evidence where the interaction between bait and prey assemble a functional beta galactosidase enzyme." ;
  rdfs:subClassOf obo:ECO_0006256 .

obo:ECO_0001042 a rdfs:Class ;
  rdfs:label "dominant-negative mutant phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence resulting from a mutated gene producing mutant polypeptides that disrupt the activity of the wild-type genes." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:GO_1902511 a rdfs:Class ;
  rdfs:label "negative regulation of apoptotic DNA fragmentation" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_1902510 , obo:GO_1903625 .

obo:OBI_0003147 a rdfs:Class ;
  rdfs:label "assay measuring quantitative binding of an epitope:MHC:TCR complex" ;
  rdfs:comment "An assay that detects the binding of a MHC:epitope complex with a T cell receptor, and produces a quantitative measurement of the binding as an output." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_1110037 .

obo:ECO_0001170 a rdfs:Class ;
  rdfs:label "cross-linking evidence used in manual assertion" ;
  rdfs:comment "A type of cross-linking evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001033 , obo:ECO_0006076 .

obo:ECO_0008081 a rdfs:Class ;
  rdfs:label "MHC complex binding prediction based MHC restriction evidence" ;
  rdfs:comment "A type of computational evidence that employs a computational tool to predict MHC complex binding to a ligand to infer that T cells can recognize the ligand when presented by that MHC molecule." ;
  rdfs:subClassOf obo:ECO_0007672 .

obo:OBI_0001053 a rdfs:Class ;
  rdfs:label "electrophoresis system" ;
  rdfs:comment "A device that moves charged particles through a medium by using an electric field induced by electrodes." ;
  rdfs:subClassOf obo:OBI_0000932 .

obo:OBI_0001155 a rdfs:Class ;
  rdfs:label "3D structural organization datum" ;
  rdfs:comment "A measurement datum that describes the structural orientation of a material entity in 3D space. " ;
  rdfs:subClassOf obo:IAO_0000109 .

obo:ECO_0007807 a rdfs:Class ;
  rdfs:label "protein localization evidence used in automatic assertion" ;
  rdfs:comment "A type of protein localization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000122 , obo:ECO_0007806 .

obo:OBI_0000731 a rdfs:Class ;
  rdfs:label "recombinant vector" ;
  rdfs:comment "A recombinant vector is created by a recombinant vector cloning process, and contains nucleic acids that can be amplified. It retains functions of the original cloning vector." ;
  rdfs:subClassOf obo:BFO_0000040 , obo:OBI_0000047 .

obo:ECO_0007072 a rdfs:Class ;
  rdfs:label "flow cell biofilm assay evidence used in manual assertion" ;
  rdfs:comment "A type of flow cell biofilm assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006021 , obo:ECO_0007071 .

obo:OBI_0001635 a rdfs:Class ;
  rdfs:label "antibody binding detection by fluorescence quenching" ;
  rdfs:comment "A binding assay in which affinity is measured by detecting a change in fluorescence (usually quenching) that occurs upon binding of the antibody to the ligand. The fluorescent signal that is affected by binding is either from an exogenous fluorophore attached to the ligand, or is the intrisic fluorescence of aromatic (tryptophan) residues on the binding site of the antibody (no conjugated fluorophore necessary) or, less commonly, on the ligand binding region (epitope)." ;
  rdfs:subClassOf obo:OBI_0001979 .

obo:ECO_0007126 a rdfs:Class ;
  rdfs:label "454 pyrosequencing evidence used in manual assertion" ;
  rdfs:comment "A type of 454 pyrosequencing evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000223 , obo:ECO_0000269 .

obo:ECO_0008040 a rdfs:Class ;
  rdfs:label "antibody 10.3.6-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody 10.3.6 to determine that the H2-IA protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0007197 a rdfs:Class ;
  rdfs:label "voucher specimen phenotypic analysis evidence used in automatic assertion" ;
  rdfs:comment "A type of voucher specimen phenotypic analysis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000050 , obo:ECO_0007635 .

obo:ECO_0000224 a rdfs:Class ;
  rdfs:label "SOLiD sequencing evidence" ;
  rdfs:comment "A type of high throughput nucleotide sequencing evidence in which sequence is determined through rounds of ligation-based sequencing with fluorescently-labeled Di-base probes and cleavage, after which the template is reset, with a primer offset by one base, for subsequent rounds of ligation." ;
  rdfs:subClassOf obo:ECO_0000221 .

obo:ECO_0000305 a rdfs:Class ;
  rdfs:label "curator inference used in manual assertion" ;
  rdfs:comment "A type of curator inference that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/ic-inferred-curator> ;
  rdfs:subClassOf obo:ECO_0000205 , obo:ECO_0007751 .

obo:ECO_0007768 a rdfs:Class ;
  rdfs:label "transcription assay evidence used in automatic assertion" ;
  rdfs:comment "A type of transcription assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000168 , obo:ECO_0007307 .

obo:GO_0006950 a rdfs:Class ;
  rdfs:label "response to stress" ;
  rdfs:subClassOf obo:GO_0050896 .

obo:ECO_0000314 a rdfs:Class ;
  rdfs:label "direct assay evidence used in manual assertion" ;
  rdfs:comment "A type of direct assay evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/ida-inferred-direct-assay> ;
  rdfs:subClassOf obo:ECO_0000002 , obo:ECO_0000269 .

obo:GO_0060148 a rdfs:Class ;
  rdfs:label "positive regulation of posttranscriptional gene silencing" ;
  rdfs:subClassOf obo:GO_0048518 , obo:GO_0060147 , obo:GO_0065007 .

obo:ECO_0007157 a rdfs:Class ;
  rdfs:label "cell staining evidence used in manual assertion" ;
  rdfs:comment "A type of cell staining evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001603 , obo:ECO_0007695 .

obo:ECO_0001138 a rdfs:Class ;
  rdfs:label "3D cell culture evidence used in manual assertion" ;
  rdfs:comment "A type of 3D cell culture evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001001 , obo:ECO_0007696 .

obo:ECO_0007651 a rdfs:Class ;
  rdfs:label "automatically integrated combinatorial computational evidence" ;
  rdfs:comment "A type of automatically integrated combinatorial evidence in which two or more distinct types of computational evidence are integrated automatically." ;
  rdfs:subClassOf obo:ECO_0007673 , obo:ECO_0007677 .

obo:ECO_0007676 a rdfs:Class ;
  rdfs:label "manually integrated combinatorial evidence used in automatic assertion" ;
  rdfs:comment "A type of manually integrated combinatorial evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000213 , obo:ECO_0007674 .

obo:ECO_0008109 a rdfs:Class ;
  rdfs:label "antibody B7/21-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody B7/21-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008050 , obo:ECO_0008087 .

obo:ECO_0001116 a rdfs:Class ;
  rdfs:label "protein dot blot assay evidence" ;
  rdfs:comment "A type of protein detection assay evidence resulting from protein samples immobilized on a membrane, then subsequently incubated and imaged." ;
  rdfs:subClassOf obo:ECO_0005517 .

obo:IAO_0000102 a rdfs:Class ;
  rdfs:label "data about an ontology part"@en ;
  rdfs:comment "Data about an ontology part is a data item about a part of an ontology, for example a term"@en ;
  rdfs:subClassOf obo:IAO_0000027 .

obo:ECO_0005582 a rdfs:Class ;
  rdfs:label "cell-detached outside-out single-channel recording evidence" ;
  rdfs:comment "A type of patch-clamp recording evidence where a part of cellular membrane is detached from the cell and whose external face is exposed to the extracellular medium which allows isolating and studying a single or at least a small number of channels into that fragment (patch)." ;
  rdfs:subClassOf obo:ECO_0006012 .

obo:GO_0033043 a rdfs:Class ;
  rdfs:label "regulation of organelle organization" ;
  rdfs:subClassOf obo:GO_0051128 , obo:GO_0065007 .

obo:ECO_0007117 a rdfs:Class ;
  rdfs:label "mutant physiological response evidence used in manual assertion" ;
  rdfs:comment "A type of mutant physiological response evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000174 , obo:ECO_0000315 .

obo:ECO_0008049 a rdfs:Class ;
  rdfs:label "antibody B22-249.R1-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody B22-249.R1 to determine that the H2-Db protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:OBI_0001501 a rdfs:Class ;
  rdfs:label "fluorescence detection assay" ;
  rdfs:comment "An assay in which a material's fluorescence is determined." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007757 a rdfs:Class ;
  rdfs:label "curator inference from book used in manual assertion" ;
  rdfs:comment "A type of curator inference from book that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007646 , obo:ECO_0007752 .

obo:OBI_0400044 a rdfs:Class ;
  rdfs:label "flow cytometer" ;
  rdfs:comment "A flow_cytometer is an instrument for counting, examining and sorting microscopic particles in suspension. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of single cells flowing through an optical and/or electronic detection apparatus. A flow cytometer is an instrument that can be used to quantitatively measure the properties of individual cells in a flowing medium." ;
  rdfs:subClassOf obo:OBI_0400137 .

obo:ECO_0006184 a rdfs:Class ;
  rdfs:label "small-angle neutron scattering evidence" ;
  rdfs:comment "A type of particle scattering evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight and structural transitions by irradiating the sample with a beam of thermal neutrons and the scattered intensity is probed at small angles (typically in the range of 0.1-10 degrees) by a detector." ;
  rdfs:subClassOf obo:ECO_0006183 .

obo:GO_1902512 a rdfs:Class ;
  rdfs:label "positive regulation of apoptotic DNA fragmentation" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_1902510 , obo:GO_1903626 .

obo:ECO_0001197 a rdfs:Class ;
  rdfs:label "in vitro farnesylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro farnesylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001064 .

obo:ECO_0000364 a rdfs:Class ;
  rdfs:label "evidence based on logical inference from manual annotation used in automatic assertion" ;
  rdfs:comment "A type of evidence based on logical inference from a manually curated annotation that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000363 .

obo:ECO_0001203 a rdfs:Class ;
  rdfs:label "in vitro sumoylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro sumoylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001070 .

obo:ECO_0007687 a rdfs:Class ;
  rdfs:label "protein kinase assay evidence used in manual assertion" ;
  rdfs:comment "A type of protein kinase assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001558 , obo:ECO_0005801 .

obo:ECO_0008013 a rdfs:Class ;
  rdfs:label "neural network method evidence" ;
  rdfs:comment "A type of machine learning method evidence in which the machine learning model consists of nodes connected to each other with edges that are organized into layers, where the edges can transmit signals between nodes such that the system acts similarly to biological neurons." ;
  rdfs:subClassOf obo:ECO_0008000 .

obo:ECO_0007603 a rdfs:Class ;
  rdfs:label "neutralization test assay evidence used in automatic assertion" ;
  rdfs:comment "A type of neutralization test assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007033 , obo:ECO_0007348 .

obo:ECO_0001585 a rdfs:Class ;
  rdfs:label "small interfering RNA knockdown evidence used in manual assertion" ;
  rdfs:comment "A type of small interfering RNA knockdown evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001583 , obo:ECO_0006050 .

obo:ECO_0000053 a rdfs:Class ;
  rdfs:label "automatically integrated combinatorial evidence used in automatic assertion" ;
  rdfs:comment "A type of automatically integrated combinatorial evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000213 , obo:ECO_0007673 .

obo:ECO_0007351 a rdfs:Class ;
  rdfs:label "blood test evidence used in automatic assertion" ;
  rdfs:comment "A type of blood test evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001016 , obo:ECO_0007307 .

obo:ECO_0007420 a rdfs:Class ;
  rdfs:label "in vivo phosphatase assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo phosphatase assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001084 , obo:ECO_0007772 .

obo:ECO_0000067 a rdfs:Class ;
  rdfs:label "developmental similarity evidence" ;
  rdfs:comment "A type of phenotypic similarity evidence based on the similarity of embryological or post-embryonic orgin of structures." ;
  rdfs:subClassOf obo:ECO_0000057 .

obo:ECO_0007168 a rdfs:Class ;
  rdfs:label "translocation mutation phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of translocation mutation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0005515 .

obo:ECO_0007400 a rdfs:Class ;
  rdfs:label "in vitro farnesylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro farnesylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001064 , obo:ECO_0007800 .

obo:ECO_0008141 a rdfs:Class ;
  rdfs:label "antibody M1/42-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody M1/42-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008066 , obo:ECO_0008087 .

obo:ECO_0007152 a rdfs:Class ;
  rdfs:label "single exon transcript confirmation via alignment evidence used in manual assertion" ;
  rdfs:comment "A type of single exon transcript confirmation via alignment evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000247 , obo:ECO_0000345 .

obo:ECO_0007303 a rdfs:Class ;
  rdfs:label "transcript expression evidence used in automatic assertion" ;
  rdfs:comment "A type of transcript expression evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000009 , obo:ECO_0007298 .

obo:ECO_0006343 a rdfs:Class ;
  rdfs:label "quantitative macroscopy evidence" ;
  rdfs:comment "A type of macroscopy evidence in which measurement techniques result in numerical data." ;
  rdfs:subClassOf obo:ECO_0001094 .

obo:ECO_0001105 a rdfs:Class ;
  rdfs:label "nuclear magnetic resonance evidence" ;
  rdfs:comment "A type of magnetic resonance evidence in which atomic nuclei in a strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting electromagnetic signal is captured." ;
  rdfs:subClassOf obo:BFO_0000002 , obo:ECO_0006252 .

obo:ECO_0001035 a rdfs:Class ;
  rdfs:label "cytochemistry evidence" ;
  rdfs:comment "A type of histochemistry evidence resulting from localizing chemical components of cells and organelles on histological sections." ;
  rdfs:subClassOf obo:ECO_0001050 .

obo:GO_0043555 a rdfs:Class ;
  rdfs:label "regulation of translation in response to stress" ;
  rdfs:subClassOf obo:GO_0006417 , obo:GO_0065007 .

obo:ECO_0007680 a rdfs:Class ;
  rdfs:label "chromatography evidence used in manual assertion" ;
  rdfs:comment "A type of chromatography evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0000325 .

obo:ECO_0000257 a rdfs:Class ;
  rdfs:label "motif similarity evidence used in manual assertion" ;
  rdfs:comment "A type of motif similarity evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000028 , obo:ECO_0000255 .

obo:ECO_0007582 a rdfs:Class ;
  rdfs:label "double mutant phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of double mutant phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000054 , obo:ECO_0007325 .

obo:GO_1903963 a rdfs:Class ;
  rdfs:label "arachidonate transport" ;
  rdfs:subClassOf obo:GO_0015718 , obo:GO_0015909 , obo:GO_0071715 .

obo:ECO_0007392 a rdfs:Class ;
  rdfs:label "immunohistochemistry evidence used in automatic assertion" ;
  rdfs:comment "A type of immunohistochemistry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001055 , obo:ECO_0007819 .

obo:ECO_0005534 a rdfs:Class ;
  rdfs:label "comparative genomics motif search evidence" ;
  rdfs:comment "A type of motif similarity evidence based on comparative genomics which uses a search method to scan a set of genomes for instances of a specific motif, and then applies a comparative criterion to analyze the collective strength of these predictions, based on the assumption that functional instances of the motif will be preserved by natural selection and their prediction will therefore be consistent across genomes." ;
  rdfs:subClassOf obo:ECO_0000028 .

obo:ECO_0007130 a rdfs:Class ;
  rdfs:label "4C evidence used in manual assertion" ;
  rdfs:comment "A type of 4C evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000234 , obo:ECO_0000269 .

obo:CHMO_0000102 a rdfs:Class ;
  rdfs:label "light microscopy assay" ;
  rdfs:comment "Microscopy where the specimen is illuminated with visible light and a system of lenses is used to produce an image." ;
  rdfs:subClassOf obo:OBI_0002119 .

obo:ECO_0007619 a rdfs:Class ;
  rdfs:label "static light scattering assay evidence used in automatic assertion" ;
  rdfs:comment "A type of static light scattering assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007065 , obo:ECO_0007617 .

obo:ECO_0001232 a rdfs:Class ;
  rdfs:label "microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001098 , obo:ECO_0001256 .

obo:ECO_0006225 a rdfs:Class ;
  rdfs:label "cryogenic electron microscopy-based structural model with missing residue coordinates used in automatic assertion" ;
  rdfs:comment "A type of cryogenic electron microscopy-based structural model with missing residue coordinates that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006189 , obo:ECO_0006209 .

obo:GO_0051223 a rdfs:Class ;
  rdfs:label "regulation of protein transport" ;
  rdfs:subClassOf obo:GO_0051049 , obo:GO_0065007 , obo:GO_0070201 .

obo:ECO_0001214 a rdfs:Class ;
  rdfs:label "in vivo farnesylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo farnesylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001081 .

obo:ECO_0007255 a rdfs:Class ;
  rdfs:label "specific protein inhibition by antibody evidence used in automatic assertion" ;
  rdfs:comment "A type of specific protein inhibition by antibody evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000341 , obo:ECO_0007785 .

obo:ECO_0005598 a rdfs:Class ;
  rdfs:label "wide-field fluorescence microscopy evidence" ;
  rdfs:comment "A type of wide-field microscopy evidence where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter." ;
  rdfs:subClassOf obo:ECO_0005588 .

obo:ECO_0007099 a rdfs:Class ;
  rdfs:label "gene expression similarity evidence used in manual assertion" ;
  rdfs:comment "A type of gene expression similarity evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000075 , obo:ECO_0000252 .

obo:ECO_0007667 a rdfs:Class ;
  rdfs:label "automatically integrated combinatorial computational and experimental evidence used in automatic assertion" ;
  rdfs:comment "A type of automatically integrated combinatorial computational and experimental evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000053 , obo:ECO_0007665 , obo:ECO_0007829 .

obo:ECO_0006033 a rdfs:Class ;
  rdfs:label "optogenetic evidence used in manual assertion" ;
  rdfs:comment "A type of optogenetic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006032 , obo:ECO_0007634 .

obo:ECO_0000138 a rdfs:Class ;
  rdfs:label "ribohomopolymer binding assay evidence" ;
  rdfs:comment "A type of nucleic acid binding evidence resulting from an enzyme displaying binding activity to specific ribohomopolymer." ;
  rdfs:subClassOf obo:ECO_0000136 .

obo:ECO_0006002 a rdfs:Class ;
  rdfs:label "Cya fusion reporter assay evidence used in manual assertion" ;
  rdfs:comment "A type of Cya fusion reporter assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000270 , obo:ECO_0001273 , obo:ECO_0006001 .

obo:ECO_0001147 a rdfs:Class ;
  rdfs:label "anion-exchange chromatography evidence used in manual assertion" ;
  rdfs:comment "A type of anion-exchange chromatography evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001010 , obo:ECO_0007680 .

obo:OBI_0200197 a rdfs:Class ;
  rdfs:label "spectrum analysis objective" ;
  rdfs:comment "is a data transformation objective where the aim is to analyse some aspect of spectral data by some data transformation process. " ;
  rdfs:subClassOf obo:OBI_0200166 .

obo:OBI_0001364 a rdfs:Class ;
  rdfs:label "genetic alteration information"@en ;
  rdfs:comment "a genetic characteristics information that is about known changes or the lack thereof from the genetic background, including allele information, duplication, insertion, deletion, etc."@en ;
  rdfs:subClassOf obo:OBI_0001404 .

obo:ECO_0006214 a rdfs:Class ;
  rdfs:label "small-angle neutron scattering evidence used in manual assertion" ;
  rdfs:comment "A type of small-angle neutron scattering evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006184 , obo:ECO_0006212 .

obo:GO_0044248 a rdfs:Class ;
  rdfs:label "cellular catabolic process" ;
  rdfs:subClassOf obo:GO_0009056 , obo:GO_0044237 .

obo:ECO_0007211 a rdfs:Class ;
  rdfs:label "yellow fluorescent protein fusion protein localization evidence used in automatic assertion" ;
  rdfs:comment "A type of yellow fluorescent protein fusion protein localization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000128 , obo:ECO_0007808 .

obo:GO_0006997 a rdfs:Class ;
  rdfs:label "nucleus organization" ;
  rdfs:subClassOf obo:GO_0006996 .

obo:GO_0044092 a rdfs:Class ;
  rdfs:label "negative regulation of molecular function" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_0065009 .

obo:GO_1902338 a rdfs:Class ;
  rdfs:label "negative regulation of apoptotic process involved in morphogenesis" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_1902337 , obo:GO_1904746 .

obo:ECO_0007019 a rdfs:Class ;
  rdfs:label "agglutination test evidence" ;
  rdfs:comment "A type of immunological assay evidence that involves observation of visible clumping of antibody and antigen into a complex." ;
  rdfs:subClassOf obo:ECO_0000040 .

obo:ECO_0007204 a rdfs:Class ;
  rdfs:label "methylation-specific polymerase chain reaction evidence used in automatic assertion" ;
  rdfs:comment "A type of methylation-specific polymerase chain reaction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000077 , obo:ECO_0007401 .

obo:GO_0048522 a rdfs:Class ;
  rdfs:label "positive regulation of cellular process" ;
  rdfs:subClassOf obo:GO_0048518 , obo:GO_0050794 , obo:GO_0065007 .

obo:OBI_0000816 a rdfs:Class ;
  rdfs:label "micro electrode" ;
  rdfs:comment "An electrode of very fine caliber consisting usually of a fine wire or a glass tube of capillary diameter drawn to a fine point and filled with saline or a metal used in physiological experiments to stimulate or record action currents of extracellular or intracellular origin in the nervous system." ;
  rdfs:subClassOf obo:OBI_0000832 .

obo:OBI_0000601 a rdfs:Class ;
  rdfs:label "chromatography consumable" ;
  rdfs:comment "A chromatography consumable is a consumable that is used in a chromatography experiment." ;
  rdfs:subClassOf obo:OBI_0000048 .

obo:GO_0051252 a rdfs:Class ;
  rdfs:label "regulation of RNA metabolic process" ;
  rdfs:subClassOf obo:GO_0019219 , obo:GO_0060255 , obo:GO_0065007 .

obo:ECO_0006173 a rdfs:Class ;
  rdfs:label "mixed support of intron positions by RNA-sequencing alignment evidence used in automatic assertion" ;
  rdfs:comment "A type of mixed support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000347 , obo:ECO_0006172 .

obo:ECO_0008128 a rdfs:Class ;
  rdfs:label "antibody HB-164-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody HB-164-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008059 , obo:ECO_0008088 .

obo:ECO_0000252 a rdfs:Class ;
  rdfs:label "similarity evidence used in manual assertion" ;
  rdfs:comment "A type of similarity evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000041 , obo:ECO_0000352 .

obo:ECO_0007121 a rdfs:Class ;
  rdfs:label "clinical study evidence used in manual assertion" ;
  rdfs:comment "A type of clinical study evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000180 , obo:ECO_0000269 .

obo:ECO_0007141 a rdfs:Class ;
  rdfs:label "cytochalasin experiment evidence used in manual assertion" ;
  rdfs:comment "A type of cytochalasin experiment evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000299 , obo:ECO_0007634 .

obo:ECO_0006158 a rdfs:Class ;
  rdfs:label "self-reported individual's statement evidence used in automatic assertion" ;
  rdfs:comment "A type of self-reported individual's statement evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006153 , obo:ECO_0006156 .

obo:ECO_0008056 a rdfs:Class ;
  rdfs:label "antibody DK1G8-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody DK1G8 to determine that HLA protein complex with A29 serotype presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0007200 a rdfs:Class ;
  rdfs:label "compositional similarity evidence used in automatic assertion" ;
  rdfs:comment "A type of compositional similarity evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000063 , obo:ECO_0007824 .

obo:OBI_0400082 a rdfs:Class ;
  rdfs:label "photodetector" ;
  rdfs:comment "A photodetector is a device used to detect and measure the intensity of radiant energy through photoelectric action. In a cytometer, photodetectors measure either the number of photons of laser light scattered on impact with a cell (for example), or the flourescence emitted by excitation of a fluorescent dye." ;
  rdfs:subClassOf obo:OBI_0000832 .

obo:ECO_0006354 a rdfs:Class ;
  rdfs:label "Congo red birefringence evidence used in manual assertion" ;
  rdfs:comment "A type of Congo red birefringence evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006332 , obo:ECO_0006352 .

obo:ECO_0007703 a rdfs:Class ;
  rdfs:label "cell viability assay evidence used in manual assertion" ;
  rdfs:comment "A type of cell viability assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005004 , obo:ECO_0007695 .

obo:ECO_0007820 a rdfs:Class ;
  rdfs:label "small molecule detection assay evidence used in automatic assertion" ;
  rdfs:comment "A type of small molecule detection assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001522 , obo:ECO_0007816 .

obo:ECO_0006383 a rdfs:Class ;
  rdfs:label "sexual sporulation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of sexual sporulation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006346 , obo:ECO_0006379 .

obo:ECO_0008078 a rdfs:Class ;
  rdfs:label "protein expression-based MHC presentation evidence" ;
  rdfs:comment "A type of protein expression evidence that employs MHC molecule motif or alleles present to determine that a specific MHC molecule or class of MHC molecules presented a ligand." ;
  rdfs:subClassOf obo:ECO_0000010 .

obo:OBI_0001706 a rdfs:Class ;
  rdfs:label "assay measuring biological activity resulting from B cell epitope:antibody binding" ;
  rdfs:comment "A B cell epitope assay that measures the immune response process resulting from the binding of an antibody receptor to epitope or recognition of the epitope." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_0003127 .

obo:UBERON_0000479 a rdfs:Class ;
  rdfs:label "tissue" ;
  rdfs:comment "Multicellular anatomical structure that consists of many cells of one or a few types, arranged in an extracellular matrix such that their long-range organisation is at least partly a repetition of their short-range organisation." ;
  rdfs:subClassOf obo:UBERON_0000465 .

obo:ECO_0005635 a rdfs:Class ;
  rdfs:label "genomic systematic evolution of ligands by exponential amplification evidence used in automatic assertion" ;
  rdfs:comment "A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001812 , obo:ECO_0007510 .

obo:GO_0032409 a rdfs:Class ;
  rdfs:label "regulation of transporter activity" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_0065009 .

obo:OBI_0000015 a rdfs:Class ;
  rdfs:label "biological feature identification objective"@en ;
  rdfs:comment "Biological_feature_identification_objective is an objective role carried out by the proposition defining the aim of a study designed to examine or characterize a particular biological feature."@en ;
  rdfs:subClassOf obo:IAO_0000005 .

obo:ECO_0006348 a rdfs:Class ;
  rdfs:label "host penetration assay evidence" ;
  rdfs:comment "A type of host colonization assay evidence in which the extent of penetration into host tissue is measured." ;
  rdfs:subClassOf obo:ECO_0001830 .

obo:ECO_0007814 a rdfs:Class ;
  rdfs:label "high throughput nucleotide sequencing assay evidence used in automatic assertion" ;
  rdfs:comment "A type of high throughput nucleotide sequencing assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000221 , obo:ECO_0007813 .

obo:ECO_0007222 a rdfs:Class ;
  rdfs:label "mutant visible phenotype evidence used in automatic assertion" ;
  rdfs:comment "A type of mutant visible phenotype evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000176 , obo:ECO_0007325 .

obo:ECO_0006203 a rdfs:Class ;
  rdfs:label "synchrotron radiation circular dichroism evidence used in automatic assertion" ;
  rdfs:comment "A type of synchrotron radiation circular dichroism evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006178 , obo:ECO_0006201 .

obo:ECO_0005597 a rdfs:Class ;
  rdfs:label "immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005595 , obo:ECO_0005596 , obo:ECO_0006003 .

obo:GO_0048583 a rdfs:Class ;
  rdfs:label "regulation of response to stimulus" ;
  rdfs:subClassOf obo:GO_0050789 , obo:GO_0065007 .

obo:OBI_0000924 a rdfs:Class ;
  rdfs:label "labeled specimen" ;
  rdfs:comment "A specimen  that has been modified in order to be able to detect it in future experiments" ;
  rdfs:subClassOf obo:OBI_0000953 , obo:OBI_0001936 , obo:OBI_0100051 .

obo:ECO_0005516 a rdfs:Class ;
  rdfs:label "molecule detection assay evidence" ;
  rdfs:comment "A type of experimental evidence resulting from the detection and analysis of molecular markers." ;
  rdfs:subClassOf obo:BFO_0000002 , obo:ECO_0000006 .

obo:ECO_0001063 a rdfs:Class ;
  rdfs:label "in vitro desumoylation assay evidence" ;
  rdfs:comment "A type of desumoylation assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001551 .

obo:ECO_0000090 a rdfs:Class ;
  rdfs:label "immunogold labelling evidence" ;
  rdfs:comment "A type of immunolocalization evidence where the antibodies are labeled with colloidal gold particles whose location is then detected via microscopy." ;
  rdfs:subClassOf obo:ECO_0000087 .

obo:ECO_0008130 a rdfs:Class ;
  rdfs:label "antibody HB180-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody HB180-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008060 , obo:ECO_0008088 .

obo:ECO_0001096 a rdfs:Class ;
  rdfs:label "mass spectrometry evidence" ;
  rdfs:comment "A type of spectrometry evidence resulting from identifying the amount and type of material entities present in a sample by fragmenting it and measuring the mass-to-charge ratio of the resulting particles." ;
  rdfs:subClassOf obo:BFO_0000002 , obo:ECO_0005504 .

obo:ECO_0001127 a rdfs:Class ;
  rdfs:label "surface plasmon resonance evidence" ;
  rdfs:comment "A type of physical interaction evidence based on real-time, rapid plasmon generation on the interface between a planar surface and vacuum that measures changes in refractive index close to the sensor surface when an analyte and its immobilized ligand bind to observe and characterize molecular interaction." ;
  rdfs:subClassOf obo:ECO_0000021 .

obo:ECO_0000056 a rdfs:Class ;
  rdfs:label "epistatic interaction phenotypic evidence" ;
  rdfs:comment "A type of genetic interaction evidence resulting from the suppression of one allelic effect by an allele at another genetic locus." ;
  rdfs:subClassOf obo:ECO_0000011 .

obo:ECO_0000249 a rdfs:Class ;
  rdfs:label "sequence similarity evidence used in automatic assertion" ;
  rdfs:comment "A type of sequence similarity evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000044 , obo:ECO_0000251 , obo:ECO_0007669 .

obo:ECO_0006332 a rdfs:Class ;
  rdfs:label "Congo red birefringence evidence" ;
  rdfs:comment "A type of Congo red staining evidence where the Congo red dye produces an apple-green birefringence when it specifically binds to cross-β-sheets of amyloids which is detectable via cross-polarized light." ;
  rdfs:subClassOf obo:ECO_0006331 .

obo:ECO_0007078 a rdfs:Class ;
  rdfs:label "colony morphology phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of colony morphology phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007077 , obo:ECO_0007634 .

obo:ECO_0005019 a rdfs:Class ;
  rdfs:label "staining evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from the use of stains or dyes to aid in analysis of a microscopic image." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007474 a rdfs:Class ;
  rdfs:label "electrophoretic mobility shift assay evidence used in automatic assertion" ;
  rdfs:comment "A type of electrophoretic mobility shift assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000096 , obo:ECO_0007332 .

obo:GO_0031325 a rdfs:Class ;
  rdfs:label "positive regulation of cellular metabolic process" ;
  rdfs:subClassOf obo:GO_0009893 , obo:GO_0031323 , obo:GO_0048522 , obo:GO_0065007 .

obo:ECO_0007441 a rdfs:Class ;
  rdfs:label "nuclear magnetic resonance evidence used in automatic assertion" ;
  rdfs:comment "A type of nuclear magnetic resonance evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001105 , obo:ECO_0006286 .

obo:ECO_0000263 a rdfs:Class ;
  rdfs:label "transmembrane domain prediction evidence used in automatic assertion" ;
  rdfs:comment "A type of transmembrane domain prediction that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000083 , obo:ECO_0000258 .

obo:ECO_0005646 a rdfs:Class ;
  rdfs:label "interferometric reflectance imaging sensor evidence used in automatic assertion" ;
  rdfs:comment "A type of interferometric reflectance imaging sensor evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005520 , obo:ECO_0007816 .

obo:ECO_0008002 a rdfs:Class ;
  rdfs:label "rule-based machine learning method evidence" ;
  rdfs:comment "A type of machine learning method evidence which uses a combination of data attributes to derive knowledge used to create a set of rules. These rules collectively represent the prediction model and are in the form of \"IF:THEN\" statements. The knowledge applied can be traced back to the rules and the original data." ;
  rdfs:subClassOf obo:ECO_0008000 .

obo:ECO_0000052 a rdfs:Class ;
  rdfs:label "suppressor/enhancer interaction phenotypic evidence" ;
  rdfs:comment "A type of genetic interaction evidence resulting from a second mutation that either suppresses or enhances the phenotype expressed from an original mutation." ;
  rdfs:subClassOf obo:ECO_0000011 .

obo:ECO_0007618 a rdfs:Class ;
  rdfs:label "dynamic light scattering assay evidence used in automatic assertion" ;
  rdfs:comment "A type of dynamic light scattering assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007063 , obo:ECO_0007617 .

obo:ECO_0000289 a rdfs:Class ;
  rdfs:label "spatial pattern of transcript expression evidence used in manual assertion" ;
  rdfs:comment "A type of spatial pattern of transcript expression analysis that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000047 , obo:ECO_0000291 .

obo:ECO_0007645 a rdfs:Class ;
  rdfs:label "curator inference from journal publication" ;
  rdfs:comment "A type of curator inference from published work reporting on research findings." ;
  rdfs:subClassOf obo:ECO_0007647 .

obo:ECO_0006372 a rdfs:Class ;
  rdfs:label "molecular dynamics evidence used in manual assertion" ;
  rdfs:comment "A type of molecular dynamics evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006340 , obo:ECO_0007668 .

obo:ECO_0001824 a rdfs:Class ;
  rdfs:label "DNA adenine methyltransferase identification evidence" ;
  rdfs:comment "A type of affinity evidence used to identify DNA binding sites in eukaryotes where Escherichia coli DNA adenine methyltransferase (Dam) is fused to a transcription factor, co-factor, chromatin-associated protein, or nuclear-associated protein, followed by a methyl-dependent PCR to localize methyltransferase in the region of the binding site." ;
  rdfs:subClassOf obo:ECO_0000023 .

obo:ECO_0005660 a rdfs:Class ;
  rdfs:label "quantitative polymerase chain reaction evidence used in manual assertion" ;
  rdfs:comment "A type of quantitative polymerase chain reaction evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000231 , obo:ECO_0000269 .

obo:OBI_0000369 a rdfs:Class ;
  rdfs:label "magnify function"@en ;
  rdfs:comment "A magnify function is a function to increase the size of a transmitted object image through the precise arrangement of energy diffraction elements along an imaging path."@en ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:ECO_0007234 a rdfs:Class ;
  rdfs:label "4C evidence used in automatic assertion" ;
  rdfs:comment "A type of 4C evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000234 , obo:ECO_0007822 .

obo:ECO_0007408 a rdfs:Class ;
  rdfs:label "in vitro translation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro translation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001072 , obo:ECO_0007799 .

obo:GO_0003690 a rdfs:Class ;
  rdfs:label "double-stranded DNA binding" ;
  rdfs:subClassOf obo:GO_0003677 .

obo:OBI_0001861 a rdfs:Class ;
  rdfs:label "methylation-sensitive restriction enzyme sequencing assay" ;
  rdfs:comment "An assay that identifies unmethylated CpGs using methylation sensitive restriction enzymes to fragment DNA." ;
  rdfs:subClassOf obo:OBI_0000626 , obo:OBI_0001266 .

obo:OBI_0000664 a rdfs:Class ;
  rdfs:label "BrdU incorporation assay" ;
  rdfs:comment "A cell proliferation assay in which cells are cultured in the presence of BrdU which is incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication, which can be quantified by BrdU specific antibodies." ;
  rdfs:subClassOf obo:OBI_0000891 .

obo:ECO_0008154 a rdfs:Class ;
  rdfs:label "antibody SF1-1.1.1-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody SF1-1.1.1-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008072 , obo:ECO_0008088 .

obo:ECO_0007013 a rdfs:Class ;
  rdfs:label "combinatorial experimental and author inference evidence used in manual assertion" ;
  rdfs:comment "A type of combinatorial evidence from author knowledge and experimental evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000244 , obo:ECO_0007011 .

obo:ECO_0005508 a rdfs:Class ;
  rdfs:label "silent mutation evidence" ;
  rdfs:comment "A type of point mutation phenotypic evidence resulting from the change of a DNA nucleotide that does not cause any changes in the amino acid sequence." ;
  rdfs:subClassOf obo:ECO_0001113 .

obo:ECO_0008092 a rdfs:Class ;
  rdfs:label "antibody 14.4.4-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody 14.4.4-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008041 , obo:ECO_0008088 .

obo:ECO_0007089 a rdfs:Class ;
  rdfs:label "loss-of-function mutant phenotype evidence used in manual assertion" ;
  rdfs:comment "A type of loss-of-function mutant phenotype evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000016 , obo:ECO_0000315 .

obo:ECO_0007349 a rdfs:Class ;
  rdfs:label "blocking peptide evidence used in automatic assertion" ;
  rdfs:comment "A type of blocking peptide evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001014 , obo:ECO_0007348 .

obo:ECO_0008051 a rdfs:Class ;
  rdfs:label "antibody B8.11.2-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody B8.11.2 to determine that the HLA-DR protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:OBI_0001079 a rdfs:Class ;
  rdfs:label "confocal microscope" ;
  rdfs:comment "A microscope that is used to increase micrograph contrast and/or reconstruct three-dimensional images by using a spatial pinhole to eliminate out-of-focus light in specimens that are thicker than the focal plane." ;
  rdfs:subClassOf obo:OBI_0400169 .

obo:GO_0022803 a rdfs:Class ;
  rdfs:label "passive transmembrane transporter activity" ;
  rdfs:subClassOf obo:GO_0022857 .

obo:GO_2000192 a rdfs:Class ;
  rdfs:label "negative regulation of fatty acid transport" ;
  rdfs:subClassOf obo:GO_0032369 , obo:GO_0032891 , obo:GO_0065007 , obo:GO_2000191 .

obo:ECO_0006195 a rdfs:Class ;
  rdfs:label "protein hydrogen-deuterium exchange mass spectrometry evidence" ;
  rdfs:comment "A type of mass spectrometry evidence derived from measuring changes in mass associated with the isotopic exchange between amide hydrogens of the protein backbone and its surrounding solvent, providing information on the structural state, the dynamics and the intrinsic chemical properties of the underlying amino acid sequence." ;
  rdfs:subClassOf obo:ECO_0001096 .

obo:ECO_0000213 a rdfs:Class ;
  rdfs:label "combinatorial evidence used in automatic assertion" ;
  rdfs:comment "A type of combinatorial analysis that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000212 , obo:ECO_0000501 .

obo:ECO_0008106 a rdfs:Class ;
  rdfs:label "antibody B123.2-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody B123.2-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008048 , obo:ECO_0008088 .

obo:ECO_0000283 a rdfs:Class ;
  rdfs:label "spatial pattern of protein expression evidence used in manual assertion" ;
  rdfs:comment "A type of protein expression spatial pattern analysis that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000045 , obo:ECO_0000284 .

obo:ECO_0001074 a rdfs:Class ;
  rdfs:label "in vivo acetylation assay evidence" ;
  rdfs:comment "A type of acetylation assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001546 .

obo:GO_0051240 a rdfs:Class ;
  rdfs:label "positive regulation of multicellular organismal process" ;
  rdfs:subClassOf obo:GO_0048518 , obo:GO_0051239 , obo:GO_0065007 .

obo:ECO_0007662 a rdfs:Class ;
  rdfs:label "manually integrated combinatorial computational and experimental evidence" ;
  rdfs:comment "A type of manually integrated combinatorial evidence in which at least one line of experimental evidence and at least one line of computational evidence have been integrated manually." ;
  rdfs:subClassOf obo:ECO_0007661 , obo:ECO_0007674 .

obo:OBI_0000385 a rdfs:Class ;
  rdfs:label "ionization function"@en ;
  rdfs:comment "An ionization function is a function to physically convert an atom or molecule into an ion by adding or removing charged particles such as electrons or other ions."@en ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:ECO_0005519 a rdfs:Class ;
  rdfs:label "DNA detection assay evidence" ;
  rdfs:comment "A type of molecule detection assay evidence resulting from the detection and visualization of a sequence of DNA." ;
  rdfs:subClassOf obo:ECO_0005516 .

obo:GO_0010942 a rdfs:Class ;
  rdfs:label "positive regulation of cell death" ;
  rdfs:subClassOf obo:GO_0010941 , obo:GO_0048522 , obo:GO_0065007 .

obo:OBI_0003128 a rdfs:Class ;
  rdfs:label "T cell epitope assay" ;
  rdfs:comment "An immune epitope assay that characterizes the structrue of an epitope-T cell receptor complex, or measures the binding of a T cell receptor to epitope, or the immune response process resulting from such a binding event or the recognition of the epitope." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_1110128 .

obo:ECO_0001172 a rdfs:Class ;
  rdfs:label "cytochemistry evidence used in manual assertion" ;
  rdfs:comment "A type of cytochemistry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001035 , obo:ECO_0001185 .

obo:ECO_0007226 a rdfs:Class ;
  rdfs:label "in vitro assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000181 , obo:ECO_0007307 .

obo:ECO_0006141 a rdfs:Class ;
  rdfs:label "quantum mechanics simulation evidence" ;
  rdfs:comment "A type of computational evidence where the behavior of matter and light on the atomic and subatomic scale is described." ;
  rdfs:subClassOf obo:ECO_0007672 .

obo:ECO_0007528 a rdfs:Class ;
  rdfs:label "ad-hoc qualitative phenotype observation evidence used in automatic assertion" ;
  rdfs:comment "A type of ad-hoc qualitative phenotype observation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005673 , obo:ECO_0007635 .

obo:ECO_0008143 a rdfs:Class ;
  rdfs:label "antibody M5/114-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody M5/114-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008067 , obo:ECO_0008087 .

obo:ECO_0007024 a rdfs:Class ;
  rdfs:label "direct Coombs test evidence used in manual assertion" ;
  rdfs:comment "A type of direct Coombs test evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007020 , obo:ECO_0007023 .

obo:ECO_0006162 a rdfs:Class ;
  rdfs:label "medical practitioner statement evidence used in automatic assertion" ;
  rdfs:comment "A type of medical practitioner statement evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006152 , obo:ECO_0006156 .

obo:OBI_1110022 a rdfs:Class ;
  rdfs:label "adaptive immune receptor"@en ;
  rdfs:comment "is a receptor produced by cells of the adaptive immune system with the purpose of binding epitopes."@en ;
  rdfs:subClassOf obo:GO_0032991 .

obo:ECO_0006224 a rdfs:Class ;
  rdfs:label "cryogenic electron microscopy-based structural model with missing residue coordinates used in manual assertion" ;
  rdfs:comment "A type of cryogenic electron microscopy-based structural model with missing residue coordinates that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006189 , obo:ECO_0006208 .

obo:GO_0009890 a rdfs:Class ;
  rdfs:label "negative regulation of biosynthetic process" ;
  rdfs:subClassOf obo:GO_0009889 , obo:GO_0009892 , obo:GO_0065007 .

obo:ECO_0007179 a rdfs:Class ;
  rdfs:label "electroencephalography recording evidence used in manual assertion" ;
  rdfs:comment "A type of electroencephalography recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005577 , obo:ECO_0006006 .

obo:GO_0072749 a rdfs:Class ;
  rdfs:label "cellular response to cytochalasin B" ;
  rdfs:subClassOf obo:GO_0071407 , obo:GO_0071417 , obo:GO_1901328 , obo:GO_1901701 .

obo:GO_0002833 a rdfs:Class ;
  rdfs:label "positive regulation of response to biotic stimulus" ;
  rdfs:subClassOf obo:GO_0002831 , obo:GO_0048584 , obo:GO_0065007 .

obo:ECO_0006156 a rdfs:Class ;
  rdfs:label "documented statement evidence used in automatic assertion" ;
  rdfs:comment "A type of documented statement evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000501 , obo:ECO_0006151 .

obo:ECO_0008067 a rdfs:Class ;
  rdfs:label "antibody M5/114-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody M5/114 to determine that the mouse MHC class II protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0000272 a rdfs:Class ;
  rdfs:label "Affymetrix GeneChip evidence used in manual assertion" ;
  rdfs:comment "A type of Affymetrix GeneChip evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000101 , obo:ECO_0000276 .

obo:ECO_0007163 a rdfs:Class ;
  rdfs:label "insertion mutation phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of insertion mutation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0005509 .

obo:ECO_0007110 a rdfs:Class ;
  rdfs:label "thin layer chromatography evidence used in manual assertion" ;
  rdfs:comment "A type of thin layer chromatography evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000140 , obo:ECO_0007680 .

obo:ECO_0001060 a rdfs:Class ;
  rdfs:label "in vitro deacetylation assay evidence" ;
  rdfs:comment "A type of deacetylation assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001548 .

obo:ECO_0006275 a rdfs:Class ;
  rdfs:label "analytical ultracentrifugation evidence used in manual assertion" ;
  rdfs:comment "A type of analytical ultracentrifugation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0006247 .

obo:OBI_0001146 a rdfs:Class ;
  rdfs:label "binding assay" ;
  rdfs:comment "An assay with the objective to characterize the disposition of two or more material entities to form a complex." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007629 a rdfs:Class ;
  rdfs:label "Etest evidence used in automatic assertion" ;
  rdfs:comment "A type of Etest evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007085 , obo:ECO_0007628 .

obo:OBI_0000626 a rdfs:Class ;
  rdfs:label "DNA sequencing assay" ;
  rdfs:comment "A sequencing assay which determines information on the sequence of a DNA molecule." ;
  rdfs:subClassOf obo:OBI_0600047 .

obo:ECO_0001015 a rdfs:Class ;
  rdfs:label "blocking polyclonal antibody evidence" ;
  rdfs:comment "A type of immunological assay evidence resulting from the inhibitory effect of polyclonal antibodies (antibodies secreted by different B cell lineages), which while not reacting with a specific antigen, serve to prevent other antibodies from doing so." ;
  rdfs:subClassOf obo:ECO_0000040 .

obo:ECO_0006093 a rdfs:Class ;
  rdfs:label "transient rescue experiment evidence used in manual assertion" ;
  rdfs:comment "A type of transient rescue experiment evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000038 , obo:ECO_0006091 .

obo:ECO_0000184 a rdfs:Class ;
  rdfs:label "enzyme inhibition evidence" ;
  rdfs:comment "A type of protein inhibition evidence and enzymatic acitivty evidence where the protein under study is an enzyme." ;
  rdfs:subClassOf obo:ECO_0000005 , obo:ECO_0000020 .

obo:ECO_0007502 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation-PCR evidence used in automatic assertion" ;
  rdfs:comment "A type of chromatin immunoprecipitation-PCR evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000227 , obo:ECO_0007786 .

obo:ECO_0008152 a rdfs:Class ;
  rdfs:label "antibody PT85-A-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody PT85-A-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008071 , obo:ECO_0008088 .

obo:ECO_0001024 a rdfs:Class ;
  rdfs:label "chimeric protein phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence resulting from the fusion of two or more different genes to create one protein product." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:SO_0000001 a rdfs:Class ;
  rdfs:label "region" ;
  rdfs:comment "A sequence_feature with an extent greater than zero. A nucleotide region is composed of bases and a polypeptide region is composed of amino acids." ;
  rdfs:subClassOf obo:BFO_0000031 .

obo:ECO_0006235 a rdfs:Class ;
  rdfs:label "selective antibody-based structural conformation evidence used in automatic assertion" ;
  rdfs:comment "A type of selective antibody-based structural conformation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006194 , obo:ECO_0007796 , obo:ECO_0007815 .

obo:ECO_0007721 a rdfs:Class ;
  rdfs:label "farnesylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of farnesylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001553 .

obo:OBI_0000618 a rdfs:Class ;
  rdfs:label "size exclusion column" ;
  rdfs:comment "A size exclusion column is a chromatography column as used in size exclusion chromatography and which enables the separation of mixtures according to differrences in molecular size." ;
  rdfs:subClassOf obo:OBI_0000038 .

obo:GO_0006355 a rdfs:Class ;
  rdfs:label "regulation of transcription, DNA-templated" ;
  rdfs:subClassOf obo:GO_0010468 , obo:GO_0065007 , obo:GO_1903506 .

obo:ECO_0007539 a rdfs:Class ;
  rdfs:label "author statement supported from referenced clinical study used in automatic assertion" ;
  rdfs:comment "A type of author statement supported from referenced clinical study that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006016 , obo:ECO_0007321 .

obo:ECO_0000325 a rdfs:Class ;
  rdfs:label "chromatography evidence" ;
  rdfs:comment "A type of experimental evidence that is based on separation of constituent parts of a mixture (the mobile phase) as they pass differentially through a stationary phase due to differences in partition coefficient and retention on the stationary phase." ;
  rdfs:subClassOf obo:BFO_0000002 , obo:ECO_0000006 .

obo:ECO_0006264 a rdfs:Class ;
  rdfs:label "competitive binding evidence" ;
  rdfs:comment "A type of affinity evidence derived from an experimental setup where the binding properties of a ligand are assessed by measuring the competition with another ligand with already known binding properties." ;
  rdfs:subClassOf obo:ECO_0000023 .

obo:ECO_0001158 a rdfs:Class ;
  rdfs:label "cell permeability assay evidence used in manual assertion" ;
  rdfs:comment "A type of cell permeability assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001021 , obo:ECO_0007695 .

obo:ECO_0000112 a rdfs:Class ;
  rdfs:label "qualitative western immunoblotting evidence" ;
  rdfs:comment "A type of protein expression evidence where proteins are separated using gel electrophoresis, blotted onto a membrane, and probed with an antibody that can be detected via light or radioactive emission." ;
  rdfs:subClassOf obo:ECO_0000010 , obo:ECO_0000333 , obo:ECO_0005593 .

obo:GO_2001020 a rdfs:Class ;
  rdfs:label "regulation of response to DNA damage stimulus" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_0080135 .

obo:ECO_0007730 a rdfs:Class ;
  rdfs:label "cleavage arrested development evidence used in manual assertion" ;
  rdfs:comment "A type of cleavage arrested development evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000298 , obo:ECO_0007634 .

obo:IAO_0000032 a rdfs:Class ;
  rdfs:label "scalar measurement datum"@en ;
  rdfs:comment "A scalar measurement datum is a measurement datum that is composed of two parts, numerals and a unit label."@en ;
  rdfs:subClassOf obo:IAO_0000109 .

obo:ECO_0006337 a rdfs:Class ;
  rdfs:label "stopped-flow fluorescence spectroscopy evidence" ;
  rdfs:comment "A type of fluorescence evidence used to study binding kinetics by rapidly mixing two interaction components and then \"stopping\" the reaction in the observation chamber." ;
  rdfs:subClassOf obo:ECO_0000021 , obo:ECO_0001115 .

obo:ECO_0001551 a rdfs:Class ;
  rdfs:label "desumoylation assay evidence" ;
  rdfs:comment "A type of direct assay evidence where the removal of sumo groups from a protein is detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0001835 a rdfs:Class ;
  rdfs:label "infection assay evidence used in manual assertion" ;
  rdfs:comment "A type of infection assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001832 , obo:ECO_0001833 .

obo:ECO_0001004 a rdfs:Class ;
  rdfs:label "51Cr release assay evidence" ;
  rdfs:comment "A type of cytotoxicity assay evidence resulting from the measurement of the amount of radioactive 51Cr released in the supernatant of a sample of 51Cr pre-labeled cells via cytolysis by effector cells (cytotoxic T-cells)." ;
  rdfs:subClassOf obo:ECO_0005012 .

obo:ECO_0007715 a rdfs:Class ;
  rdfs:label "polyADP-ribosylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of polyADP-ribosylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001557 .

obo:ECO_0007446 a rdfs:Class ;
  rdfs:label "peptide array evidence used in automatic assertion" ;
  rdfs:comment "A type of peptide array evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001112 , obo:ECO_0007373 .

obo:OBI_1110001 a rdfs:Class ;
  rdfs:label "epitope"@en ;
  rdfs:comment "a material entity bearing the epitope role"@en ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:ECO_0007698 a rdfs:Class ;
  rdfs:label "apoptotic assay evidence used in manual assertion" ;
  rdfs:comment "A type of apoptotic assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005034 , obo:ECO_0007695 .

obo:ECO_0007186 a rdfs:Class ;
  rdfs:label "tethered cell assay evidence used in manual assertion" ;
  rdfs:comment "A type of tethered cell assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005607 , obo:ECO_0007701 .

obo:ECO_0007093 a rdfs:Class ;
  rdfs:label "voucher specimen phenotypic analysis evidence used in manual assertion" ;
  rdfs:comment "A type of voucher specimen phenotypic analysis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000050 , obo:ECO_0007634 .

obo:ECO_0001221 a rdfs:Class ;
  rdfs:label "in vivo translation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo translation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001088 .

obo:OBI_0001956 a rdfs:Class ;
  rdfs:label "assay using chromatin immunoprecipitation" ;
  rdfs:comment "An assay that produces data about protein-DNA interaction or DNA epigenetic modification using immunoprecipitation" ;
  rdfs:subClassOf obo:OBI_0001700 .

obo:ECO_0000018 a rdfs:Class ;
  rdfs:label "anti-sense experiment evidence" ;
  rdfs:comment "A type of mutant phenotype evidence where a phenotype is observed while expressing an anti-sense version of a gene product in a wild-type (for that gene product) background." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0007383 a rdfs:Class ;
  rdfs:label "eTag assay evidence used in automatic assertion" ;
  rdfs:comment "A type of eTag assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001045 , obo:ECO_0007384 .

obo:ECO_0006326 a rdfs:Class ;
  rdfs:label "viscosity measurement evidence used in automatic assertion" ;
  rdfs:comment "A type of viscosity measurement evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006272 , obo:ECO_0007307 .

obo:ECO_0006013 a rdfs:Class ;
  rdfs:label "patch-clamp recording evidence used in manual assertion" ;
  rdfs:comment "A type of patch-clamp recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006006 , obo:ECO_0006012 .

obo:ECO_0007083 a rdfs:Class ;
  rdfs:label "zone of inhibition evidence" ;
  rdfs:comment "A type of cell growth assay evidence resulting from assessing the sensitivity or resistance of bacteria or fungi to an antimicrobial agent or chemical by measuring the size of the zone of inhibition that results when the test organism is grown on a solid surface in the presence of the antimicrobial agent or chemical." ;
  rdfs:subClassOf obo:ECO_0001563 .

obo:GO_0048646 a rdfs:Class ;
  rdfs:label "anatomical structure formation involved in morphogenesis" ;
  rdfs:subClassOf obo:GO_0032502 .

obo:ECO_0007003 a rdfs:Class ;
  rdfs:label "high throughput genetic interaction phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of high throughput genetic interaction phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000316 , obo:ECO_0006056 , obo:ECO_0007002 .

obo:ECO_0007164 a rdfs:Class ;
  rdfs:label "duplication mutation evidence used in manual assertion" ;
  rdfs:comment "A type of duplication mutation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005511 , obo:ECO_0007634 .

obo:OBI_0002169 a rdfs:Class ;
  rdfs:label "ChIP-qPCR assay" ;
  rdfs:comment "A ChIP assay that uses quantitative PCR to determine levels of specific DNA in immunoprecipitated samples." ;
  rdfs:subClassOf obo:OBI_0001954 , obo:OBI_0002692 .

obo:OBI_0002177 a rdfs:Class ;
  rdfs:label "patch clamp assay" ;
  rdfs:comment "An intracellular electrophysiology assay where a glass micropipette is sealed to the surface of the cell membrane as a recording electrode to study ion channel activity. The key distinction of this technique is the electrical resistance of the seal between the cell membrane and the pipette is of the order 10-100 gigaohms, permitting high-resolution current measurements over the cell membrane in several different standard configurations." ;
  rdfs:subClassOf obo:OBI_0000447 .

obo:ECO_0007312 a rdfs:Class ;
  rdfs:label "spatial pattern of protein expression evidence used in automatic assertion" ;
  rdfs:comment "A type of spatial pattern of protein expression evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000045 , obo:ECO_0007309 .

obo:ECO_0000294 a rdfs:Class ;
  rdfs:label "bacterial one-hybrid evidence" ;
  rdfs:comment "A type of bait-prey hybrid interaction evidence that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey)." ;
  rdfs:subClassOf obo:ECO_0000025 .

obo:ECO_0007345 a rdfs:Class ;
  rdfs:label "annexin-V staining evidence used in automatic assertion" ;
  rdfs:comment "A type of annexin-V staining evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001011 , obo:ECO_0007777 .

obo:OBI_0003024 a rdfs:Class ;
  rdfs:label "lymphocyte assay" ;
  rdfs:comment "A cytometry assay that measures the abundance and/or characteristics of lymphocytes" ;
  rdfs:subClassOf obo:OBI_0001977 .

obo:GO_0031330 a rdfs:Class ;
  rdfs:label "negative regulation of cellular catabolic process" ;
  rdfs:subClassOf obo:GO_0009895 , obo:GO_0031324 , obo:GO_0031329 , obo:GO_0065007 .

obo:ECO_0007523 a rdfs:Class ;
  rdfs:label "S1 nuclease protection assay evidence used in automatic assertion" ;
  rdfs:comment "A type of S1 nuclease protection assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005521 , obo:ECO_0007316 .

obo:ECO_0007558 a rdfs:Class ;
  rdfs:label "RNAi evidence used in automatic assertion" ;
  rdfs:comment "A type of RNAi evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000019 , obo:ECO_0007325 .

obo:ECO_0007362 a rdfs:Class ;
  rdfs:label "co-localization evidence used in automatic assertion" ;
  rdfs:comment "A type of co-localization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001026 , obo:ECO_0007363 .

obo:ECO_0001188 a rdfs:Class ;
  rdfs:label "immunodepletion evidence used in manual assertion" ;
  rdfs:comment "A type of immunodepletion evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001054 , obo:ECO_0006078 .

obo:ECO_0006048 a rdfs:Class ;
  rdfs:label "field potential recording evidence used in manual assertion" ;
  rdfs:comment "A type of field potential recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006044 , obo:ECO_0006047 .

obo:ECO_0000360 a rdfs:Class ;
  rdfs:label "biological target-disease association via drug evidence" ;
  rdfs:comment "A type of experimental evidence resulting from the prediction of drug-target interactions by computational means." ;
  rdfs:subClassOf obo:ECO_0000006 .

obo:ECO_0000347 a rdfs:Class ;
  rdfs:label "support of intron positions by RNA-sequencing alignment evidence used in automatic assertion" ;
  rdfs:comment "A type of support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000342 , obo:ECO_0006069 .

obo:ECO_0007153 a rdfs:Class ;
  rdfs:label "phylogenetic distribution evidence used in manual assertion" ;
  rdfs:comment "A type of phylogenetic distribution evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000252 , obo:ECO_0000355 .

obo:ECO_0007710 a rdfs:Class ;
  rdfs:label "defarnesylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of defarnesylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001549 .

obo:ECO_0001571 a rdfs:Class ;
  rdfs:label "colony diameter phenotype evidence" ;
  rdfs:comment "A type of colony size phenotypic evidence resulting from measurement of a microbial colony's diameter." ;
  rdfs:subClassOf obo:ECO_0007081 .

obo:ECO_0006253 a rdfs:Class ;
  rdfs:label "electron paramagnetic resonance evidence" ;
  rdfs:comment "A type of magnetic resonance evidence in which unpaired electrons in a strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting electromagnetic signal is captured." ;
  rdfs:subClassOf obo:ECO_0006252 .

obo:ECO_0007276 a rdfs:Class ;
  rdfs:label "sustained current evidence used in automatic assertion" ;
  rdfs:comment "A type of sustained current evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005566 , obo:ECO_0007535 .

obo:ECO_0005567 a rdfs:Class ;
  rdfs:label "obsolete steady state activation curve evidence" .

obo:ECO_0007785 a rdfs:Class ;
  rdfs:label "protein inhibition evidence used in automatic assertion" ;
  rdfs:comment "A type of protein inhibition evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000020 , obo:ECO_0007307 .

obo:ECO_0008174 a rdfs:Class ;
  rdfs:label "inference of MHC restriction based on shared MHC expression used in automatic assertion" ;
  rdfs:comment "A type of inference of MHC restriction based on shared MHC expression that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007832 , obo:ECO_0008082 .

obo:GO_1904797 a rdfs:Class ;
  rdfs:label "negative regulation of core promoter binding" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_1904796 , obo:GO_2000678 .

obo:ECO_0007607 a rdfs:Class ;
  rdfs:label "epifluorescence microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of epifluorescence microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006324 , obo:ECO_0007041 .

obo:ECO_0005552 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence by experimental evidence from mixed species" ;
  rdfs:comment "A type of biological system reconstruction evidence where the experimental evidence is derived by using a mix of species used in the same experiment." ;
  rdfs:subClassOf obo:ECO_0005551 .

obo:GO_0007569 a rdfs:Class ;
  rdfs:label "cell aging" ;
  rdfs:subClassOf obo:GO_0007568 , obo:GO_0048869 .

obo:ECO_0007185 a rdfs:Class ;
  rdfs:label "cross-streak test evidence used in manual assertion" ;
  rdfs:comment "A type of cross-streak test evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005604 , obo:ECO_0007696 .

obo:ECO_0001192 a rdfs:Class ;
  rdfs:label "in vitro deacetylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro deacetylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001060 .

obo:UBERON_0006314 a rdfs:Class ;
  rdfs:label "bodily fluid" ;
  rdfs:comment "Liquid components of living organisms. includes fluids that are excreted or secreted from the body as well as body water that normally is not." ;
  rdfs:subClassOf obo:UBERON_0000463 .

obo:ECO_0008045 a rdfs:Class ;
  rdfs:label "antibody 34-7-23S-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody 34-7-23S to determine that the H2-Dd protein complex and/or H2-Kd protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0006288 a rdfs:Class ;
  rdfs:label "electron paramagnetic resonance evidence used in automatic assertion" ;
  rdfs:comment "A type of electron paramagnetic resonance evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006253 , obo:ECO_0006286 .

obo:ECO_0007419 a rdfs:Class ;
  rdfs:label "in vivo palmitoylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo palmitoylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001083 , obo:ECO_0007792 .

obo:ECO_0006246 a rdfs:Class ;
  rdfs:label "colony boundary assay evidence used in automatic assertion" ;
  rdfs:comment "A type of colony boundary assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006240 , obo:ECO_0007625 .

obo:ECO_0005801 a rdfs:Class ;
  rdfs:label "enzymatic activity assay evidence used in manual assertion"@en ;
  rdfs:comment "A type of enzyme assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000005 , obo:ECO_0000314 .

obo:ECO_0006213 a rdfs:Class ;
  rdfs:label "particle scattering evidence used in automatic assertion" ;
  rdfs:comment "A type of particle scattering evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006183 , obo:ECO_0007815 .

obo:ECO_0000160 a rdfs:Class ;
  rdfs:label "protein separation followed by fragment identification evidence" ;
  rdfs:comment "A type of protein separation evidence which then utilizes the screening and identification of small-molecules that bind to proteins." ;
  rdfs:subClassOf obo:ECO_0000156 .

obo:GO_0000976 a rdfs:Class ;
  rdfs:label "transcription cis-regulatory region binding" ;
  rdfs:subClassOf obo:GO_0001067 , obo:GO_1990837 .

obo:ECO_0007297 a rdfs:Class ;
  rdfs:label "experimental evidence used in automatic assertion" ;
  rdfs:comment "A type of experimental evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000006 , obo:ECO_0000501 .

obo:ECO_0007839 a rdfs:Class ;
  rdfs:label "curator inference from dictionary used in automatic assertion" ;
  rdfs:comment "A type of curator inference from dictionary that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007641 , obo:ECO_0007833 .

obo:ECO_0006059 a rdfs:Class ;
  rdfs:label "high throughput cell biology evidence used in manual assertion" ;
  rdfs:comment "A type of high throughput evidence used in a manual assertion where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel." ;
  rdfs:subClassOf obo:ECO_0006056 , obo:ECO_0006058 .

obo:PATO_0000018 a rdfs:Class ;
  rdfs:label "fluorescence" ;
  rdfs:comment "A luminous flux quality inhering in a bearer by virtue of the bearer's emitting longer wavelength light following the absorption of shorter wavelength radiation; fluorescence is common with aromatic compounds with several rings joined together." ;
  rdfs:subClassOf obo:PATO_0001296 .

obo:OBI_0000406 a rdfs:Class ;
  rdfs:label "PCR product"@en ;
  rdfs:comment "is double stranded DNA that is the specified output of a polymerase chain reaction"@en ;
  rdfs:subClassOf obo:CHEBI_4705 , obo:OBI_0000809 .

obo:ECO_0000041 a rdfs:Class ;
  rdfs:label "similarity evidence" ;
  rdfs:comment "A type of evidence resulting from comparing likeness of distinct biological entities." ;
  rdfs:subClassOf obo:ECO_0000000 .

obo:OBI_0001978 a rdfs:Class ;
  rdfs:label "immunoblot assay" ;
  rdfs:comment "An analyte assay that detects specific molecules in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with_ antibodies specific to the analyte molecules." ;
  rdfs:subClassOf obo:OBI_0000443 .

obo:ECO_0000042 a rdfs:Class ;
  rdfs:label "gain-of-function mutant phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence resulting from an altered gene product which possesses a new molecular function or a new pattern of gene expression." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0007426 a rdfs:Class ;
  rdfs:label "obsolete induced mutation evidence used in automatic assertion" ;
  rdfs:comment "OBSOLETE. A type of induced mutation evidence that is used in an automatic assertion." .

obo:OBI_0000112 a rdfs:Class ;
  rdfs:label "specimen role"@en ;
  rdfs:comment "a role borne by a material entity that is gained during a specimen collection process and that can be realized by use of the specimen in an investigation"@en ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:ECO_0007586 a rdfs:Class ;
  rdfs:label "recessive mutant phenotype evidence used in automatic assertion" ;
  rdfs:comment "A type of recessive mutant phenotype evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006100 , obo:ECO_0007325 .

obo:OBI_0400137 a rdfs:Class ;
  rdfs:label "cytometer" ;
  rdfs:comment "A cytometer is an instrument for counting and/or measuring characteristics of cells." ;
  rdfs:subClassOf obo:OBI_0000832 .

obo:OBI_0600045 a rdfs:Class ;
  rdfs:label "3D structure determination assay" ;
  rdfs:comment "An assay that determines the 3-dimensional configuration of an input material." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0000219 a rdfs:Class ;
  rdfs:label "nucleotide sequencing assay evidence" ;
  rdfs:comment "A type of sequencing assay that determines the sequence of a biopolymer comprised of nucleotides." ;
  rdfs:subClassOf obo:ECO_0000220 .

obo:GO_0010605 a rdfs:Class ;
  rdfs:label "negative regulation of macromolecule metabolic process" ;
  rdfs:subClassOf obo:GO_0009892 , obo:GO_0060255 , obo:GO_0065007 .

obo:ECO_0007215 a rdfs:Class ;
  rdfs:label "in vitro recombinant protein transcription reconstitution assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro recombinant protein transcription reconstitution assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000152 , obo:ECO_0007804 .

obo:ECO_0007307 a rdfs:Class ;
  rdfs:label "direct assay evidence used in automatic assertion" ;
  rdfs:comment "A type of direct assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000002 , obo:ECO_0007297 .

obo:OBI_0002621 a rdfs:Class ;
  rdfs:label "reconstitution assay" ;
  rdfs:comment "An assay in which a cellular process is replicated from the bottom-up using isolated components in order to observe how the components work together." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_0000471 .

obo:ECO_0005563 a rdfs:Class ;
  rdfs:label "macroscopic current trace evidence" ;
  rdfs:comment "A type of voltage clamp recording evidence that involves recording trace of the current (pA) going through the analyzed ion channels determined from voltage clamp current recording." ;
  rdfs:subClassOf obo:ECO_0005576 .

obo:ECO_0005624 a rdfs:Class ;
  rdfs:label "consensus search evidence used in manual assertion" ;
  rdfs:comment "A type of consensus search evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000257 , obo:ECO_0005532 .

obo:ECO_0000330 a rdfs:Class ;
  rdfs:label "coding sequence splice pattern evidence used in manual assertion" ;
  rdfs:comment "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in manual assertion." ;
  rdfs:subClassOf obo:ECO_0000247 , obo:ECO_0000328 .

obo:ECO_0001263 a rdfs:Class ;
  rdfs:label "urine test evidence used in manual assertion" ;
  rdfs:comment "A type of urine test evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001134 .

obo:ECO_0000000 a rdfs:Class ;
  rdfs:label "evidence" ;
  rdfs:comment "A type of information that is used to support an assertion." .

obo:ECO_0001557 a rdfs:Class ;
  rdfs:label "polyADP-ribosylation assay evidence" ;
  rdfs:comment "A type of direct assay evidence where ADP-ribosylated residues in proteins are detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0001091 a rdfs:Class ;
  rdfs:label "knockout phenotypic evidence" ;
  rdfs:comment "A type of genetic transformation evidence resulting from a change in the DNA that results in the elimination of the function of the gene, allowing for functional analysis." ;
  rdfs:subClassOf obo:ECO_0005027 .

obo:ECO_0006299 a rdfs:Class ;
  rdfs:label "Raman spectroscopy evidence used in manual assertion" ;
  rdfs:comment "A type of Raman spectroscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001258 , obo:ECO_0006259 .

obo:ECO_0000030 a rdfs:Class ;
  rdfs:label "BLAST evidence used in manual assertion" ;
  rdfs:comment "A type of BLAST evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000206 , obo:ECO_0005560 .

obo:ECO_0007495 a rdfs:Class ;
  rdfs:label "immunoperoxidase immunolocalization electron microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005596 , obo:ECO_0007493 , obo:ECO_0007494 .

obo:ECO_0007378 a rdfs:Class ;
  rdfs:label "4',6-diamidino-2-phenylindole staining evidence used in automatic assertion" ;
  rdfs:comment "A type of 4',6-diamidino-2-phenylindole staining evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001037 , obo:ECO_0007798 .

obo:ECO_0007338 a rdfs:Class ;
  rdfs:label "[3H]-thymidine incorporation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of [3H]-thymidine incorporation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001003 , obo:ECO_0007776 .

obo:OBI_0001010 a rdfs:Class ;
  rdfs:label "nucleic acid extract" ;
  rdfs:comment "An extract that is the output of an extraction process in which nucleic acid molecules are isolated from a specimen." ;
  rdfs:subClassOf obo:OBI_0000423 , obo:OBI_0000953 .

obo:GO_0051234 a rdfs:Class ;
  rdfs:label "establishment of localization" ;
  rdfs:subClassOf obo:GO_0051179 .

obo:ECO_0007522 a rdfs:Class ;
  rdfs:label "rapid amplification of cDNA ends polymerase chain reaction evidence used in automatic assertion" ;
  rdfs:comment "A type of rapid amplification of cDNA ends polymerase chain reaction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001820 , obo:ECO_0007813 .

obo:ECO_0001085 a rdfs:Class ;
  rdfs:label "in vivo protein kinase assay evidence" ;
  rdfs:comment "A type of protein kinase assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001558 .

obo:ECO_0007115 a rdfs:Class ;
  rdfs:label "two-electrode voltage clamp recording evidence used in manual assertion" ;
  rdfs:comment "A type of two-electrode voltage clamp recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000166 , obo:ECO_0006006 .

obo:ECO_0007571 a rdfs:Class ;
  rdfs:label "affinity chromatography evidence used in automatic assertion" ;
  rdfs:comment "A type of affinity chromatography evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000079 , obo:ECO_0007384 .

obo:ECO_0007803 a rdfs:Class ;
  rdfs:label "reconstitution assay evidence used in automatic assertion" ;
  rdfs:comment "A type of reconstitution assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000003 , obo:ECO_0007307 .

obo:ECO_0001079 a rdfs:Class ;
  rdfs:label "in vivo deubiquitination assay evidence" ;
  rdfs:comment "A type of deubiquitination assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001552 .

obo:ECO_0001150 a rdfs:Class ;
  rdfs:label "blocking monoclonal antibody evidence used in manual assertion" ;
  rdfs:comment "A type of blocking monoclonal antibody evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001013 , obo:ECO_0006078 .

obo:ECO_0007608 a rdfs:Class ;
  rdfs:label "transmission electron microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of transmission electron microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007043 , obo:ECO_0007493 .

obo:OBI_0302716 a rdfs:Class ;
  rdfs:label "pool of specimens"@en ;
  rdfs:comment "A pool of specimens is a mixture of a population of samples which have been gathered from one or more sample populations, obtained by the physical process of mixing individual specimens, e.g.  mixing the DNA collected from the individual fish."@en ;
  rdfs:subClassOf obo:OBI_0000953 , obo:OBI_0100051 .

obo:GO_0045184 a rdfs:Class ;
  rdfs:label "establishment of protein localization" ;
  rdfs:subClassOf obo:GO_0008104 , obo:GO_0051234 .

obo:ECO_0006296 a rdfs:Class ;
  rdfs:label "beta galactosidase functional complementation evidence used in automatic assertion" ;
  rdfs:comment "A type of beta galactosidase functional complementation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006257 , obo:ECO_0006294 .

obo:GO_0060194 a rdfs:Class ;
  rdfs:label "regulation of antisense RNA transcription" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_0140747 .

obo:ECO_0007389 a rdfs:Class ;
  rdfs:label "histology evidence used in automatic assertion" ;
  rdfs:comment "A type of histology evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001051 , obo:ECO_0007363 .

obo:ECO_0008103 a rdfs:Class ;
  rdfs:label "antibody AG2.42.7-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody AG2.42.7-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008047 , obo:ECO_0008087 .

obo:ECO_0007792 a rdfs:Class ;
  rdfs:label "palmitoylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of palmitoylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001555 , obo:ECO_0007307 .

obo:PATO_0001291 a rdfs:Class ;
  rdfs:label "electromagnetic (EM) radiation quality" ;
  rdfs:comment "A physical quality that inheres in an bearer by virtue of how that bearer interacts with electromagnetic radiation." ;
  rdfs:subClassOf obo:PATO_0001739 .

obo:ECO_0007844 a rdfs:Class ;
  rdfs:label "radioisotope quantification assay evidence" ;
  rdfs:comment "A type of radioisotope assay evidence in which radioisotopic labeling is used to quantify a substance after a biochemical process or extraction from a biological sample." ;
  rdfs:subClassOf obo:ECO_0001120 .

obo:GO_0032640 a rdfs:Class ;
  rdfs:label "tumor necrosis factor production" ;
  rdfs:subClassOf obo:GO_0071706 .

obo:ECO_0001210 a rdfs:Class ;
  rdfs:label "in vivo defarnesylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo defarnesylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001077 .

obo:ECO_0006357 a rdfs:Class ;
  rdfs:label "thioflavin T based fluorescence spectroscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of thioflavin T based fluorescence spectroscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006333 , obo:ECO_0007450 .

obo:ECO_0007266 a rdfs:Class ;
  rdfs:label "silent mutation evidence used in automatic assertion" ;
  rdfs:comment "A type of silent mutation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005508 , obo:ECO_0007448 .

obo:OBI_0000231 a rdfs:Class ;
  rdfs:label "enzymatic cleavage"@en ;
  rdfs:comment "a material processing that digests the fraction of input material that is susceptible to that enzyme"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0007656 a rdfs:Class ;
  rdfs:label "manually integrated combinatorial experimental evidence used in manual assertion" ;
  rdfs:comment "A type of manually integrated combinatorial experimental evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007655 , obo:ECO_0007675 .

obo:OBI_0666666 a rdfs:Class ;
  rdfs:label "RNA extraction"@en ;
  rdfs:comment "A RNA extraction is a nucleic acid extraction where the desired output material is RNA"@en ;
  rdfs:subClassOf obo:OBI_0666667 .

obo:OBI_0000997 a rdfs:Class ;
  rdfs:label "growth environment" ;
  rdfs:comment "The collection of material entities and their qualities that are located near a live organism, tissue or cell and can influence its growth." ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:GO_0120033 a rdfs:Class ;
  rdfs:label "negative regulation of plasma membrane bounded cell projection assembly" ;
  rdfs:subClassOf obo:GO_0031345 , obo:GO_0065007 , obo:GO_0120032 .

obo:CHEBI_50076 a rdfs:Class ;
  rdfs:label "chromium-51" ;
  rdfs:comment "A synthetic radioactive isotope of chromium having a half-life of 27.7 days and decaying by electron capture with emission of gamma rays (0.32 MeV); it is used to label red blood cells for measurement of mass or volume, survival time, and sequestration studies, for the diagnosis of gastrointestinal bleeding, and to label platelets to study their survival." ;
  rdfs:subClassOf obo:CHEBI_23367 .

obo:GO_0051336 a rdfs:Class ;
  rdfs:label "regulation of hydrolase activity" ;
  rdfs:subClassOf obo:GO_0050790 , obo:GO_0065007 .

obo:ECO_0001031 a rdfs:Class ;
  rdfs:label "conditional knockin evidence" ;
  rdfs:comment "A type of knockin evidence resulting from the targeted introduction of a temporally-restricted and/or tissue-specific mutation." ;
  rdfs:subClassOf obo:ECO_0001090 .

obo:ECO_0001816 a rdfs:Class ;
  rdfs:label "green fluorescent protein reporter gene assay evidence" ;
  rdfs:comment "A type of reporter gene assay evidence based on the fusion of select genes with the green fluorescent protein (GFP) gene for detection with bioluminescence of the gene product when exposed to blue ultraviolet light." ;
  rdfs:subClassOf obo:ECO_0000049 .

obo:OBI_0000552 a rdfs:Class ;
  rdfs:label "reverse transcribed polymerase chain reaction" ;
  rdfs:comment "reverse transcribe pcr is a process which allow amplification of cDNA during a pcr reaction while the cDNA results from a retrotranscription of messenger RNA isolated from a material entity." ;
  rdfs:subClassOf obo:OBI_0600058 .

obo:ECO_0007327 a rdfs:Class ;
  rdfs:label "genomic context evidence used in automatic assertion" ;
  rdfs:comment "A type of genomic context evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000177 , obo:ECO_0000251 .

obo:OBI_0001258 a rdfs:Class ;
  rdfs:label "ChIP-seq design" ;
  rdfs:comment "A study design which aims to identify protein and DNA interactions using a combination of chromatin immunoprecipitation and high throughput sequencing. Massively parallel sequence analyses are used in conjunction with whole-genome sequence databases to analyze the interaction pattern of any protein with DNA, or the pattern of any epigenetic chromatin modifications." ;
  rdfs:subClassOf obo:OBI_0001425 .

obo:ECO_0006376 a rdfs:Class ;
  rdfs:label "quantitative macroscopy evidence used in manual assertion" ;
  rdfs:comment "A type of quantitative macroscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001228 , obo:ECO_0006343 .

obo:ECO_0005653 a rdfs:Class ;
  rdfs:label "northern blot evidence used in manual assertion" ;
  rdfs:comment "A type of northern blot evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000106 , obo:ECO_0000291 .

obo:ECO_0006361 a rdfs:Class ;
  rdfs:label "fluorescence correlation spectroscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of fluorescence correlation spectroscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006335 , obo:ECO_0007450 .

obo:ECO_0006285 a rdfs:Class ;
  rdfs:label "magnetic resonance evidence used in manual assertion" ;
  rdfs:comment "A type of magnetic resonance evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0006252 .

obo:ECO_0006268 a rdfs:Class ;
  rdfs:label "temperature-induced protein unfolding evidence" ;
  rdfs:comment "A type of protein unfolding evidence where the unfolding of the protein is triggered by changes in temperature." ;
  rdfs:subClassOf obo:ECO_0006265 .

obo:ECO_0001546 a rdfs:Class ;
  rdfs:label "acetylation assay evidence" ;
  rdfs:comment "A type of direct assay evidence where acetylated residues are detected in a protein." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0008187 a rdfs:Class ;
  rdfs:label "single-molecule fluorescence resonance energy transfer evidence" ;
  rdfs:comment "A type of fluorescence resonance energy transfer evidence resulting from the measurement of the distance-dependent energy transfer of two fluorophores attached to individual molecules, allowing for the detection of conformational states at both spatial and temporal resolution." ;
  rdfs:subClassOf obo:ECO_0001048 .

obo:GO_0044237 a rdfs:Class ;
  rdfs:label "cellular metabolic process" ;
  rdfs:subClassOf obo:GO_0008152 , obo:GO_0009987 .

obo:ECO_0008117 a rdfs:Class ;
  rdfs:label "antibody C7709A2-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody C7709A2-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008054 , obo:ECO_0008087 .

obo:ECO_0005587 a rdfs:Class ;
  rdfs:label "confocal microscopy evidence" ;
  rdfs:comment "A type of microscopy evidence where sharp images are taken by excluding most of the light from the specimen that is not from the microscope's focal plane, thus allowing better contrast and less haze, which makes it possible to build three-dimensional reconstructions of a volume of the specimen by assembling a series of thin slices taken along the vertical axis." ;
  rdfs:subClassOf obo:ECO_0001098 .

obo:OBI_0600058 a rdfs:Class ;
  rdfs:label "enzymatic amplification"@en ;
  rdfs:comment "the use of enzymes to increase the number of molecules of a biomaterial"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0007008 a rdfs:Class ;
  rdfs:label "radioligand binding assay evidence" ;
  rdfs:comment "A type of protein binding evidence in which radioactive ligands are used to measure receptor-ligand interactions, such as in determining selectivity for a particular ligand." ;
  rdfs:subClassOf obo:ECO_0000024 .

obo:ECO_0001805 a rdfs:Class ;
  rdfs:label "luciferase reporter gene assay evidence" ;
  rdfs:comment "A type of reporter gene assay evidence where luciferase, an oxidative enzyme, is used as the reporter to detect a gene product with bioluminescence." ;
  rdfs:subClassOf obo:ECO_0000049 .

obo:ECO_0007485 a rdfs:Class ;
  rdfs:label "immunogold labelling evidence used in automatic assertion" ;
  rdfs:comment "A type of immunogold labelling evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000090 , obo:ECO_0007486 .

obo:PATO_0000125 a rdfs:Class ;
  rdfs:label "mass" ;
  rdfs:comment "A physical quality that inheres in a bearer by virtue of the proportion of the bearer's amount of matter." ;
  rdfs:subClassOf obo:PATO_0001018 .

obo:ECO_0006321 a rdfs:Class ;
  rdfs:label "RNA-protein binding evidence used in manual assertion" ;
  rdfs:comment "A type of RNA-protein binding evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006076 , obo:ECO_0006083 , obo:ECO_0006270 .

obo:ECO_0007614 a rdfs:Class ;
  rdfs:label "differential interference contrast microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of differential interference contrast microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007055 , obo:ECO_0007435 .

obo:ECO_0008023 a rdfs:Class ;
  rdfs:label "regular expression method evidence used in automatic assertion" ;
  rdfs:comment "A type of regular expression method evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008012 , obo:ECO_0008021 .

obo:ECO_0007553 a rdfs:Class ;
  rdfs:label "extracellular recording evidence used in automatic assertion" ;
  rdfs:comment "A type of extracellular recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006043 , obo:ECO_0007535 .

obo:ECO_0001592 a rdfs:Class ;
  rdfs:label "polysome profiling evidence" ;
  rdfs:comment "A type of experimental evidence employed to study the associations between multiple ribosomes and mRNA that utilizes density gradient centrifugation to separate out the lysate of cells of interest with the objective of isolating polysomes/polyribosomes which are complexes of an mRNA molecule and two or more ribosomes that act to translate mRNA.  The optical density of the fractions is then determined." ;
  rdfs:subClassOf obo:ECO_0000006 .

obo:ECO_0008163 a rdfs:Class ;
  rdfs:label "antibody Y3JP-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody Y3JP-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008077 , obo:ECO_0008087 .

obo:ECO_0007774 a rdfs:Class ;
  rdfs:label "cell viability assay evidence used in automatic assertion" ;
  rdfs:comment "A type of cell viability assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005004 , obo:ECO_0007773 .

obo:BFO_0000003 a rdfs:Class ;
  rdfs:label "occurrent"@en ;
  rdfs:comment "An entity that has temporal parts and that happens, unfolds or develops through time."@en ;
  rdfs:subClassOf obo:BFO_0000001 .

obo:GO_1900130 a rdfs:Class ;
  rdfs:label "regulation of lipid binding" ;
  rdfs:subClassOf obo:GO_0051098 , obo:GO_0065007 .

obo:CHEBI_33250 a rdfs:Class ;
  rdfs:label "atom" ;
  rdfs:comment "A chemical entity constituting the smallest component of an element having the chemical properties of the element." ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:ECO_0007833 a rdfs:Class ;
  rdfs:label "curator inference from authoritative source used in automatic assertion" ;
  rdfs:comment "A type of curator inference from authoritative source that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007322 , obo:ECO_0007647 .

obo:OBI_0001553 a rdfs:Class ;
  rdfs:label "assay measuring a binding constant of a MHC:ligand complex" ;
  rdfs:comment "A MHC:ligand binding assay that measures a binding constant." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_0003145 .

obo:OBI_0001032 a rdfs:Class ;
  rdfs:label "light emission device" ;
  rdfs:comment "A device which has a function to emit light." ;
  rdfs:subClassOf obo:OBI_0000968 .

obo:GO_0018130 a rdfs:Class ;
  rdfs:label "heterocycle biosynthetic process" ;
  rdfs:subClassOf obo:GO_0044249 , obo:GO_0046483 .

obo:ECO_0007825 a rdfs:Class ;
  rdfs:label "transcript splice pattern evidence used in automatic assertion" ;
  rdfs:comment "A type of transcript splice pattern evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000248 , obo:ECO_0000326 .

obo:ECO_0005603 a rdfs:Class ;
  rdfs:label "protein mass spectrometry evidence" ;
  rdfs:comment "A type of mass spectrometry evidence where one or more protein analyses are performed." ;
  rdfs:subClassOf obo:ECO_0001096 .

obo:GO_0001046 a rdfs:Class ;
  rdfs:label "core promoter sequence-specific DNA binding" ;
  rdfs:comment "Interacting selectively and non-covalently with a sequence of DNA that is part of a core promoter region. The core promoter is composed of the transcription start site and binding sites for the RNA polymerase and the basal transcription machinery. The transcribed region might be described as a gene, cistron, or operon." ;
  rdfs:subClassOf obo:GO_0000976 .

obo:ECO_0007136 a rdfs:Class ;
  rdfs:label "environmental perturbation phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of environmental perturbation phenotypic evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000241 , obo:ECO_0007634 .

obo:ECO_0006011 a rdfs:Class ;
  rdfs:label "mRNA interactome capture evidence used in manual assertion" ;
  rdfs:comment "A type of mRNA interactome capture evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006010 , obo:ECO_0006083 .

obo:GO_0019814 a rdfs:Class ;
  rdfs:label "immunoglobulin complex" ;
  rdfs:comment "A protein complex that in its canonical form is composed of two identical immunoglobulin heavy chains and two identical immunoglobulin light chains, held together by disulfide bonds and sometimes complexed with additional proteins. An immunoglobulin complex may be embedded in the plasma membrane or present in the extracellular space, in mucosal areas or other tissues, or circulating in the blood or lymph." ;
  rdfs:subClassOf obo:GO_0032991 .

obo:GO_0006928 a rdfs:Class ;
  rdfs:label "movement of cell or subcellular component" ;
  rdfs:subClassOf obo:GO_0009987 .

obo:OBI_0000930 a rdfs:Class ;
  rdfs:label "calorimeter" ;
  rdfs:comment "A measurement device that is used to calculate the heat flow of a chemical reaction or physical change." ;
  rdfs:subClassOf obo:OBI_0000832 .

obo:ECO_0008034 a rdfs:Class ;
  rdfs:label "intrinsic disorder prediction evidence used in automatic assertion" ;
  rdfs:comment "A type of intrinsic disorder prediction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007669 , obo:ECO_0008030 .

obo:GO_0031346 a rdfs:Class ;
  rdfs:label "positive regulation of cell projection organization" ;
  rdfs:subClassOf obo:GO_0031344 , obo:GO_0051130 , obo:GO_0065007 .

obo:ECO_0007732 a rdfs:Class ;
  rdfs:label "sequencing assay evidence used in manual assertion" ;
  rdfs:comment "A type of sequencing assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000220 , obo:ECO_0000269 .

obo:GO_0140097 a rdfs:Class ;
  rdfs:label "catalytic activity, acting on DNA" ;
  rdfs:subClassOf obo:GO_0140640 .

obo:OBI_0600047 a rdfs:Class ;
  rdfs:label "sequencing assay" ;
  rdfs:comment "An assay the uses chemical or biochemical means to infer the sequence of a biomaterial" ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0000230 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation-chip evidence" ;
  rdfs:comment "A type of chromatin immunoprecipitation evidence that uses a tiling microarray for the detection of protein-bound DNA regions where chromatin is immunoprecipitated for protein tagging and DNA is sheared from the cross-linked protein-DNA complex to be arrayed revealing the protein-bound genomic regions." ;
  rdfs:subClassOf obo:ECO_0000226 .

obo:GO_0006304 a rdfs:Class ;
  rdfs:label "DNA modification" ;
  rdfs:subClassOf obo:GO_0006259 , obo:GO_0043412 .

obo:ECO_0007174 a rdfs:Class ;
  rdfs:label "current clamp recording evidence used in manual assertion" ;
  rdfs:comment "A type of current clamp recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005571 , obo:ECO_0006006 .

obo:ECO_0007564 a rdfs:Class ;
  rdfs:label "fluorescence recovery after photobleaching evidence used in automatic assertion" ;
  rdfs:comment "A type of fluorescence recovery after photobleaching evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006066 , obo:ECO_0007450 .

obo:ECO_0001053 a rdfs:Class ;
  rdfs:label "immunocytochemistry evidence" ;
  rdfs:comment "A type of protein detection assay evidence resulting from the use of antibodies linked to coloring agents to localize structures in cell cultures to identify proteins." ;
  rdfs:subClassOf obo:ECO_0005517 .

obo:ECO_0007657 a rdfs:Class ;
  rdfs:label "manually integrated combinatorial experimental evidence used in automatic assertion" ;
  rdfs:comment "A type of manually integrated combinatorial experimental evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007655 , obo:ECO_0007676 , obo:ECO_0007830 .

obo:ECO_0000336 a rdfs:Class ;
  rdfs:label "competitive growth assay evidence" ;
  rdfs:comment "A type of cell proliferation assay evidence in which a mutated strain of a microorganism, such as a yeast or bacterium, is grown competitively with wild-type cells and the relative fitness of the strains is assessed usually by means of a fluorescent marker and flow cytometric analysis." ;
  rdfs:subClassOf obo:ECO_0005007 .

obo:GO_0045935 a rdfs:Class ;
  rdfs:label "positive regulation of nucleobase-containing compound metabolic process" ;
  rdfs:subClassOf obo:GO_0019219 , obo:GO_0031325 , obo:GO_0051173 , obo:GO_0065007 .

obo:ECO_0000341 a rdfs:Class ;
  rdfs:label "specific protein inhibition by antibody evidence" ;
  rdfs:comment "A type of specific protein inhibition evidence where the molecular function of a protein is inhibited by an antibody." ;
  rdfs:subClassOf obo:ECO_0000020 .

obo:OBI_0302892 a rdfs:Class ;
  rdfs:label "enzymatic ligation"@en ;
  rdfs:comment "An enzymatic ligation is a planned process in which molecules  are joined by covalent bonds through the action of an material entity with a ligase activity"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0006082 a rdfs:Class ;
  rdfs:label "affinity chromatography evidence used in manual assertion" ;
  rdfs:comment "A type of affinity evidence that is used in a manual assertion that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bond compound." ;
  rdfs:subClassOf obo:ECO_0000079 , obo:ECO_0006075 .

obo:ECO_0005541 a rdfs:Class ;
  rdfs:label "obsolete computationally derived logical inference from manual assertion used in automatic assertion" .

obo:ECO_0006136 a rdfs:Class ;
  rdfs:label "quantum mechanics/molecular mechanics simulation evidence used in automatic assertion" ;
  rdfs:comment "A type of quantum mechanics/molecular mechanics simulation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006135 .

obo:GO_0001819 a rdfs:Class ;
  rdfs:label "positive regulation of cytokine production" ;
  rdfs:subClassOf obo:GO_0001817 , obo:GO_0010628 , obo:GO_0051240 , obo:GO_0065007 .

obo:CHEBI_16991 a rdfs:Class ;
  rdfs:label "deoxyribonucleic acid" ;
  rdfs:comment "High molecular weight, linear polymers, composed of nucleotides containing deoxyribose and linked by phosphodiester bonds; DNA contain the genetic information of organisms." ;
  rdfs:subClassOf obo:CHEBI_33696 .

obo:ECO_0006097 a rdfs:Class ;
  rdfs:label "functional complementation in heterologous system evidence used in manual assertion" ;
  rdfs:comment "A type of functional complementation evidence that is used in a manual assertion that is based on the insertion of a wild-type copy of a gene into a heterologous organism, with the mutation occurring in a homologous gene." ;
  rdfs:subClassOf obo:ECO_0000064 , obo:ECO_0006091 .

obo:ECO_0001068 a rdfs:Class ;
  rdfs:label "in vitro protein kinase assay evidence" ;
  rdfs:comment "A type of protein kinase assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001558 .

obo:ECO_0001021 a rdfs:Class ;
  rdfs:label "cell permeability assay evidence" ;
  rdfs:comment "A type of cell-based assay evidence resulting from the quantification of cell permeability." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:ECO_0006279 a rdfs:Class ;
  rdfs:label "bait-prey protein pull-down evidence used in manual assertion" ;
  rdfs:comment "A type of bait-prey protein pull-down evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006249 , obo:ECO_0006392 .

obo:ECO_0008114 a rdfs:Class ;
  rdfs:label "antibody B8-24-3-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody B8-24-3-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008052 , obo:ECO_0008088 .

obo:ECO_0007253 a rdfs:Class ;
  rdfs:label "two-dimensional agarose gel electrophoresis evidence used in automatic assertion" ;
  rdfs:comment "A type of two-dimensional agarose gel electrophoresis evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000339 , obo:ECO_0007769 .

obo:ECO_0007850 a rdfs:Class ;
  rdfs:label "inference of sequence features from visual inspection" ;
  rdfs:comment "A type of inferential evidence in which sequence features are inferred based on visual inspection of the sequence." ;
  rdfs:subClassOf obo:ECO_0000361 .

obo:ECO_0007741 a rdfs:Class ;
  rdfs:label "chromosome conformation-based evidence used in manual assertion" ;
  rdfs:comment "A type of chromosome conformation-based evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000232 , obo:ECO_0000269 .

obo:ECO_0001140 a rdfs:Class ;
  rdfs:label "7-aminoactinomycin staining evidence used in manual assertion" ;
  rdfs:comment "A type of 7-aminoactinomycin staining evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001005 .

obo:ECO_0007818 a rdfs:Class ;
  rdfs:label "RNA detection assay evidence used in automatic assertion" ;
  rdfs:comment "A type of RNA detection assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005518 , obo:ECO_0007816 .

obo:GO_2000679 a rdfs:Class ;
  rdfs:label "positive regulation of transcription regulatory region DNA binding" ;
  rdfs:subClassOf obo:GO_0043388 , obo:GO_0048518 , obo:GO_0065007 , obo:GO_2000677 .

obo:ECO_0007280 a rdfs:Class ;
  rdfs:label "cell-attached single-channel recording evidence used in automatic assertion" ;
  rdfs:comment "A type of cell-attached single-channel recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005573 , obo:ECO_0007537 .

obo:GO_0042710 a rdfs:Class ;
  rdfs:label "biofilm formation" ;
  rdfs:subClassOf obo:GO_0098630 .

obo:ECO_0007372 a rdfs:Class ;
  rdfs:label "cross-linking evidence used in automatic assertion" ;
  rdfs:comment "A type of cross-linking evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001033 , obo:ECO_0007373 .

obo:ECO_0006152 a rdfs:Class ;
  rdfs:label "medical practitioner statement evidence" ;
  rdfs:comment "A type of documented statement evidence in which information is stated by a health care professional, for example, but not limited to a doctor, nurse, or psychologist." ;
  rdfs:subClassOf obo:ECO_0006151 .

obo:GO_0034641 a rdfs:Class ;
  rdfs:label "cellular nitrogen compound metabolic process" ;
  rdfs:subClassOf obo:GO_0006807 , obo:GO_0044237 .

obo:ECO_0000015 a rdfs:Class ;
  rdfs:label "mutant phenotype evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence in which an observable phenotypic difference results from a change or mutation in DNA." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0007759 a rdfs:Class ;
  rdfs:label "curator inference from database used in manual assertion" ;
  rdfs:comment "A type of curator inference from database that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007636 , obo:ECO_0007752 .

obo:OBI_0001682 a rdfs:Class ;
  rdfs:label "bacterial one-hybrid assay" ;
  rdfs:comment "An assay that identifies the sequence-specific target site of a DNA-binding domain. In this system, a given transcription factor (TF) is expressed as a fusion to a subunit of RNA polymerase. In parallel, a library of randomized oligonucleotides representing potential TF target sequences, is cloned into a separate vector containing the selectable genes HIS3 and URA3. If the DNA-binding domain (bait) binds a potential DNA target site (prey) in vivo, it will recruit RNA polymerase to the promoter and activate transcription of the reporter genes in that clone. The two reporter genes, HIS3 and URA3, allow for positive and negative selections, respectively. At the end of the process, positive clones are sequenced and examined with motif-finding tools in order to resolve the favoured DNA target sequence" ;
  rdfs:subClassOf obo:OBI_0001681 .

obo:OBI_0000857 a rdfs:Class ;
  rdfs:label "complementary nucleotide probe role" ;
  rdfs:comment "A role played by a nucleic acid molecule that is used in a planned process for its ability to bind a nucleic acid molecules with complementary nucleotide sequence" ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:GO_0032304 a rdfs:Class ;
  rdfs:label "negative regulation of icosanoid secretion" ;
  rdfs:subClassOf obo:GO_0032303 , obo:GO_0051048 , obo:GO_0065007 , obo:GO_1903792 , obo:GO_2000192 .

obo:ECO_0005505 a rdfs:Class ;
  rdfs:label "regular expression motif search evidence" ;
  rdfs:comment "A type of motif similarity evidence where the motif, a local alignment of sequences, is represented by a regular expression, which captures the variability of nucleotides at each position in a discrete form and can then be used to search genetic sequences by identifying matches to the regular expression." ;
  rdfs:subClassOf obo:ECO_0000028 .

obo:ECO_0007694 a rdfs:Class ;
  rdfs:label "phosphatase assay evidence used in manual assertion" ;
  rdfs:comment "A type of phosphatase assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001556 .

obo:ECO_0007506 a rdfs:Class ;
  rdfs:label "DNA affinity chromatography evidence used in automatic assertion" ;
  rdfs:comment "A type of DNA affinity chromatography evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001809 , obo:ECO_0007681 .

obo:OBI_1110128 a rdfs:Class ;
  rdfs:label "immune epitope assay" ;
  rdfs:comment "An assay that detects the binding of an epitope to an adaptive immune receptor, or an immune process resulting from such a binding event, or characterizes the structrue of the complex resulting from such a binding event." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0007334 a rdfs:Class ;
  rdfs:label "Edman degradation evidence used in automatic assertion" ;
  rdfs:comment "A type of Edman degradation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001044 , obo:ECO_0007819 .

obo:GO_0006935 a rdfs:Class ;
  rdfs:label "chemotaxis" ;
  rdfs:subClassOf obo:GO_0042330 .

obo:ECO_0007625 a rdfs:Class ;
  rdfs:label "colony morphology phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of colony morphology phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007077 , obo:ECO_0007635 .

obo:ECO_0006024 a rdfs:Class ;
  rdfs:label "air-liquid interface assay evidence" ;
  rdfs:comment "A type of biofilm formation assay evidence in which biofilm formation is analyzed, without staining, over 4 to 48 hours by growth on a tilted multiwell plate." ;
  rdfs:subClassOf obo:ECO_0006020 .

obo:ECO_0000031 a rdfs:Class ;
  rdfs:label "protein BLAST evidence used in manual assertion" ;
  rdfs:comment "A type of protein BLAST evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000030 , obo:ECO_0000208 .

obo:ECO_0000061 a rdfs:Class ;
  rdfs:label "quantitative trait analysis evidence" ;
  rdfs:comment "A type of phenotypic evidence that is based on a gene product that is associated with a quantitative trait locus, but has not been cloned." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0007264 a rdfs:Class ;
  rdfs:label "missense mutation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of missense mutation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005506 , obo:ECO_0007448 .

obo:GO_0071706 a rdfs:Class ;
  rdfs:label "tumor necrosis factor superfamily cytokine production" ;
  rdfs:subClassOf obo:GO_0001816 .

obo:ECO_0006324 a rdfs:Class ;
  rdfs:label "fluorescence microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of fluorescence microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006271 , obo:ECO_0007435 , obo:ECO_0007450 .

obo:ECO_0000202 a rdfs:Class ;
  rdfs:label "match to sequence model evidence" ;
  rdfs:comment "A type of sequence similarity evidence in which the function of a gene product is predicted based on a sequence-based statistical model." ;
  rdfs:subClassOf obo:ECO_0000044 .

obo:ECO_0007323 a rdfs:Class ;
  rdfs:label "inference from background scientific knowledge used in automatic assertion" ;
  rdfs:comment "A type of inference from background scientific knowledge that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000001 , obo:ECO_0007322 .

obo:ECO_0007838 a rdfs:Class ;
  rdfs:label "curator inference from book used in automatic assertion" ;
  rdfs:comment "A type of curator inference from book that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007646 , obo:ECO_0007833 .

obo:ECO_0007511 a rdfs:Class ;
  rdfs:label "glutathione S-transferase pull-down assay evidence used in automatic assertion" ;
  rdfs:comment "A type of glutathione S-transferase pull-down assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001817 , obo:ECO_0007432 .

obo:OBI_0001671 a rdfs:Class ;
  rdfs:label "electrophoretic mobility shift assay" ;
  rdfs:comment "An assay that measures information about Protein-DNA or Protein-RNA interactions using gel electrophoresis and relying on the fact the molecular interactions will cause the heterodimer to be retarded on the gel when compared to controls corresponding to protein extract alone and protein extract + neutral nucleic acid." ;
  rdfs:subClassOf obo:OBI_0001146 .

obo:GO_0051271 a rdfs:Class ;
  rdfs:label "negative regulation of cellular component movement" ;
  rdfs:subClassOf obo:GO_0048523 , obo:GO_0051270 , obo:GO_0065007 .

obo:ECO_0006035 a rdfs:Class ;
  rdfs:label "fluorescent sensor evidence used in manual assertion" ;
  rdfs:comment "A type of fluorescent sensor evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001249 , obo:ECO_0006034 .

obo:ECO_0007779 a rdfs:Class ;
  rdfs:label "disk diffusion test evidence used in automatic assertion" ;
  rdfs:comment "A type of disk diffusion test evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005605 , obo:ECO_0007628 .

obo:ECO_0001581 a rdfs:Class ;
  rdfs:label "liquid chromatography coupled with tandem mass spectrometry evidence used in automatic assertion" ;
  rdfs:comment "A type of liquid chromatography coupled with tandem mass spectrometry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001580 , obo:ECO_0007433 .

obo:ECO_0000026 a rdfs:Class ;
  rdfs:label "obsolete nucleic acid hybridization evidence" ;
  rdfs:comment "OBSOLETE. A type of experimental genomic evidence resulting from a process in which single stranded nucleic acids are allowed to interact so that complexes, or hybrids, are formed by molecules with sufficiently similar, complementary sequences." .

obo:OBI_0000750 a rdfs:Class ;
  rdfs:label "study design independent variable"@en ;
  rdfs:comment "a directive information entity that is part of a study design. Independent variables are entities whose values are selected to determine its relationship to an observed phenomenon (the dependent variable). In such an experiment, an attempt is made to find evidence that the values of the independent variable determine the values of the dependent variable (that which is being measured). The independent variable can be changed as required, and its values do not represent a problem requiring explanation in an analysis, but are taken simply as given. The dependent variable on the other hand, usually cannot be directly controlled"@en ;
  rdfs:subClassOf obo:IAO_0000033 .

obo:ECO_0001812 a rdfs:Class ;
  rdfs:label "genomic systematic evolution of ligands by exponential amplification evidence" ;
  rdfs:comment "A type of systematic evolution of ligands by exponential amplification evidence based on a restricted genomic DNA library to identify naturally occurring genomic aptamers and RNA-protein interaction networks with RNA-binding protein as bait and high-throughput sequencing." ;
  rdfs:subClassOf obo:ECO_0000293 .

obo:ECO_0007612 a rdfs:Class ;
  rdfs:label "transmitted light brightfied mircoscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of transmitted light brightfied mircoscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007051 , obo:ECO_0007435 .

obo:ECO_0005523 a rdfs:Class ;
  rdfs:label "DNA dot blot assay evidence used in manual assertion" ;
  rdfs:comment "A type of DNA dot blot assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0005522 .

obo:ECO_0001586 a rdfs:Class ;
  rdfs:label "ion trap mass spectrometry evidence" ;
  rdfs:comment "A type of mass spectrometry evidence where a combination of magnetic and/or electric fields are utilized to capture charged particles in tandem with mass spectrometry." ;
  rdfs:subClassOf obo:ECO_0001096 .

obo:OBI_0000105 a rdfs:Class ;
  rdfs:label "transplantation"@en ;
  rdfs:comment "a protocol application to replace an organ or tissue of an organism"@en ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0007427 a rdfs:Class ;
  rdfs:label "genetic transformation evidence used in automatic assertion" ;
  rdfs:comment "A type of genetic transformation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005027 , obo:ECO_0007325 .

obo:GO_1902680 a rdfs:Class ;
  rdfs:label "positive regulation of RNA biosynthetic process" ;
  rdfs:subClassOf obo:GO_0010557 , obo:GO_0031328 , obo:GO_0051254 , obo:GO_0065007 , obo:GO_2001141 .

obo:ECO_0007737 a rdfs:Class ;
  rdfs:label "DNA detection assay evidence used in manual assertion" ;
  rdfs:comment "A type of DNA detection assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0005519 .

obo:OBI_0000372 a rdfs:Class ;
  rdfs:label "material separation function"@en ;
  rdfs:comment "A material separation function is a function that increases the resolution between two or more material entities. The  to distinction between the entities is usually based on some associated physical quality."@en ;
  rdfs:subClassOf obo:BFO_0000034 .

obo:ECO_0007027 a rdfs:Class ;
  rdfs:label "direct hemagglutination assay evidence" ;
  rdfs:comment "A type of agglutination test evidence where some bacteria and viruses cross-link red blood cells and clump together." ;
  rdfs:subClassOf obo:ECO_0007019 .

obo:ECO_0005593 a rdfs:Class ;
  rdfs:label "immunodetection assay evidence" ;
  rdfs:comment "A type of direct assay evidence that involves the use of antibodies to detect biomolecules." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0006202 a rdfs:Class ;
  rdfs:label "synchrotron radiation circular dichroism evidence used in manual assertion" ;
  rdfs:comment "A type of synchrotron radiation circular dichroism evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006178 , obo:ECO_0006200 .

obo:ECO_0001090 a rdfs:Class ;
  rdfs:label "knockin evidence" ;
  rdfs:comment "A type of genetic transformation evidence resulting from the targetted replacement of a wild-type DNA sequence with a different sequence." ;
  rdfs:subClassOf obo:ECO_0005027 .

obo:ECO_0001217 a rdfs:Class ;
  rdfs:label "in vivo phosphatase assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo phosphatase assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001084 .

obo:GO_0043043 a rdfs:Class ;
  rdfs:label "peptide biosynthetic process" ;
  rdfs:subClassOf obo:GO_0006518 , obo:GO_0043604 , obo:GO_1901566 .

obo:ECO_0007815 a rdfs:Class ;
  rdfs:label "structure determination evidence used in automatic assertion" ;
  rdfs:comment "A type of structure determination evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005031 , obo:ECO_0007297 .

obo:ECO_0001108 a rdfs:Class ;
  rdfs:label "phage display evidence" ;
  rdfs:comment "A type of protein binding evidence resulting from the panning of a phage library where the individual phage displayed a different peptide or protein by fusion to coat proteins on the capsid." ;
  rdfs:subClassOf obo:ECO_0000024 .

obo:ECO_0007532 a rdfs:Class ;
  rdfs:label "Cya fusion reporter assay evidence used in automatic assertion" ;
  rdfs:comment "A type of Cya fusion reporter assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006001 , obo:ECO_0007473 , obo:ECO_0007765 .

obo:ECO_0001046 a rdfs:Class ;
  rdfs:label "filter binding assay evidence" ;
  rdfs:comment "A type of physical interaction evidence resulting from a mixture of two molecules passed through a nitrocellulose filter, where one may be immobilized on the filter, and if the immobilized molecule is capable of binding to the other, it will be retained on the filter as well." ;
  rdfs:subClassOf obo:ECO_0000021 .

obo:ECO_0006391 a rdfs:Class ;
  rdfs:label "bio-layer interferometry evidence used in automatic assertion" ;
  rdfs:comment "A type of bio-layer interferometry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006350 , obo:ECO_0007816 .

obo:GO_0040017 a rdfs:Class ;
  rdfs:label "positive regulation of locomotion" ;
  rdfs:subClassOf obo:GO_0040012 , obo:GO_0048518 , obo:GO_0065007 .

obo:GO_0051246 a rdfs:Class ;
  rdfs:label "regulation of protein metabolic process" ;
  rdfs:subClassOf obo:GO_0051171 , obo:GO_0060255 , obo:GO_0065007 , obo:GO_0080090 .

obo:ECO_0005531 a rdfs:Class ;
  rdfs:label "motif discovery evidence" ;
  rdfs:comment "A type of multiple sequence alignment evidence based on a set of algorithms that infer over-represented motifs from a set of biological sequences returning one or more local multiple sequence alignment defining the inferred motifs." ;
  rdfs:subClassOf obo:ECO_0005555 .

obo:GO_1905642 a rdfs:Class ;
  rdfs:label "negative regulation of DNA methylation" ;
  rdfs:subClassOf obo:GO_0044030 , obo:GO_0051053 , obo:GO_0065007 .

obo:ECO_0005549 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence based on homology evidence" ;
  rdfs:comment "A type of biological system reconstruction where the evidence is inferred by homology based on conservation of sequence, function, and composition from an existing experimentally supported model to a process, pathway, or complex." ;
  rdfs:subClassOf obo:ECO_0000088 .

obo:PATO_0001300 a rdfs:Class ;
  rdfs:label "optical quality" ;
  rdfs:comment "An EM radiation quality in which the EM radiation is within the fiat range of the spectrum visible deemed to be light." ;
  rdfs:subClassOf obo:PATO_0001291 .

obo:ECO_0007184 a rdfs:Class ;
  rdfs:label "protein mass spectrometry evidence used in manual assertion" ;
  rdfs:comment "A type of protein mass spectrometry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001230 , obo:ECO_0005603 .

obo:ECO_0001815 a rdfs:Class ;
  rdfs:label "copper-phenanthroline footprinting evidence" ;
  rdfs:comment "A type of nucleic acid binding evidence where nucleic acid that has been bound to protein is cleaved with 1,10-phenanthroline-copper complex resulting in a high-resolution footprint of sequence-specific protein-DNA contacts." ;
  rdfs:subClassOf obo:ECO_0000136 .

obo:ECO_0001582 a rdfs:Class ;
  rdfs:label "liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion" ;
  rdfs:comment "A type of liquid chromatography coupled with tandem mass spectrometry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001230 , obo:ECO_0001580 .

obo:ECO_0000317 a rdfs:Class ;
  rdfs:label "genomic context evidence used in manual assertion" ;
  rdfs:comment "A type of genomic context evidence that is used in a manual assertion." ;
  rdfs:seeAlso <http://geneontology.org/page/igc-inferred-genomic-context> ;
  rdfs:subClassOf obo:ECO_0000177 , obo:ECO_0000252 .

obo:GO_0014070 a rdfs:Class ;
  rdfs:label "response to organic cyclic compound" ;
  rdfs:subClassOf obo:GO_0010033 .

obo:OBI_0100051 a rdfs:Class ;
  rdfs:label "specimen"@en ;
  rdfs:comment "A material entity that has the specimen role."@en ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:GO_0032078 a rdfs:Class ;
  rdfs:label "negative regulation of endodeoxyribonuclease activity" ;
  rdfs:subClassOf obo:GO_0032071 , obo:GO_0032076 , obo:GO_0065007 .

obo:ECO_0006311 a rdfs:Class ;
  rdfs:label "protein unfolding evidence used in manual assertion" ;
  rdfs:comment "A type of protein unfolding evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0006265 .

obo:GO_0009059 a rdfs:Class ;
  rdfs:label "macromolecule biosynthetic process" ;
  rdfs:subClassOf obo:GO_0043170 , obo:GO_1901576 .

obo:ECO_0001181 a rdfs:Class ;
  rdfs:label "filter binding assay evidence used in manual assertion" ;
  rdfs:comment "A type of filter binding assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000353 , obo:ECO_0001046 .

obo:GO_0015031 a rdfs:Class ;
  rdfs:label "protein transport" ;
  rdfs:subClassOf obo:GO_0045184 , obo:GO_0071702 , obo:GO_0071705 .

obo:ECO_0001144 a rdfs:Class ;
  rdfs:label "adoptive cell transfer evidence used in manual assertion" ;
  rdfs:comment "A type of adoptive cell transfer evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001007 .

obo:ECO_0000072 a rdfs:Class ;
  rdfs:label "obsolete Sos-recruitment assay evidence" .

obo:ECO_0007438 a rdfs:Class ;
  rdfs:label "MTT assay evidence used in automatic assertion" ;
  rdfs:comment "A type of MTT assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001101 , obo:ECO_0007777 .

obo:ECO_0001243 a rdfs:Class ;
  rdfs:label "phosphoamino acid analysis evidence used in manual assertion" ;
  rdfs:comment "A type of phosphoamino acid analysis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001109 .

obo:GO_0043085 a rdfs:Class ;
  rdfs:label "positive regulation of catalytic activity" ;
  rdfs:subClassOf obo:GO_0044093 , obo:GO_0050790 , obo:GO_0065007 .

obo:ECO_0008170 a rdfs:Class ;
  rdfs:label "MHC:ligand binding based MHC restriction evidence used in automatic assertion" ;
  rdfs:comment "A type of MHC:ligand binding based MHC restriction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007373 , obo:ECO_0008080 .

obo:ECO_0006242 a rdfs:Class ;
  rdfs:label "galactokinase reporter gene assay evidence used in automatic assertion" ;
  rdfs:comment "A type of galactokinase reporter gene assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006238 , obo:ECO_0007765 .

obo:OBI_0000990 a rdfs:Class ;
  rdfs:label "electron microscope" ;
  rdfs:comment "A microscope that produces an image of an object by targeting it with an electron beam" ;
  rdfs:subClassOf obo:OBI_0400169 .

obo:ECO_0007397 a rdfs:Class ;
  rdfs:label "in vitro demethylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro demethylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001062 , obo:ECO_0007795 .

obo:GO_1903555 a rdfs:Class ;
  rdfs:label "regulation of tumor necrosis factor superfamily cytokine production" ;
  rdfs:subClassOf obo:GO_0001817 , obo:GO_0065007 .

obo:ECO_0006335 a rdfs:Class ;
  rdfs:label "fluorescence correlation spectroscopy evidence" ;
  rdfs:comment "A type of fluorescence evidence that measures fluctuations in fluorescence intensity used to detect biophysical properties of single molecules such as diffusion coefficients and hydrodynamic radii." ;
  rdfs:subClassOf obo:ECO_0001115 .

obo:ECO_0007346 a rdfs:Class ;
  rdfs:label "cognitive assay phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of cognitive assay phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001012 , obo:ECO_0007635 .

obo:ECO_0001846 a rdfs:Class ;
  rdfs:label "cell population optical density evidence used in automatic assertion" ;
  rdfs:comment "A type of cell population optical density evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001845 , obo:ECO_0007773 .

obo:BFO_0000140 a rdfs:Class ;
  rdfs:label "continuant fiat boundary"@en ;
  rdfs:subClassOf obo:BFO_0000141 .

obo:OBI_0001702 a rdfs:Class ;
  rdfs:label "immunoglobulin binding to epitope" ;
  rdfs:comment "a process of an immunoglobulin complex binding to a material entity at the immunoglobulin complementarity determining region (CDR). " ;
  rdfs:subClassOf obo:OBI_0001588 , obo:OBI_1110014 .

obo:ECO_0007517 a rdfs:Class ;
  rdfs:label "methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in automatic assertion" ;
  rdfs:comment "A type of methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001814 , obo:ECO_0007505 .

obo:CHEBI_33697 a rdfs:Class ;
  rdfs:label "ribonucleic acid" ;
  rdfs:comment "High molecular weight, linear polymers, composed of nucleotides containing ribose and linked by phosphodiester bonds; RNA is central to the synthesis of proteins." ;
  rdfs:subClassOf obo:CHEBI_33696 .

obo:GO_0022607 a rdfs:Class ;
  rdfs:label "cellular component assembly" ;
  rdfs:subClassOf obo:GO_0016043 .

obo:GO_0009300 a rdfs:Class ;
  rdfs:label "antisense RNA transcription" ;
  rdfs:subClassOf obo:GO_0042868 , obo:GO_0098781 .

obo:ECO_0007636 a rdfs:Class ;
  rdfs:label "curator inference from database" ;
  rdfs:comment "A type of curator inference from authoritative resource based on information located in a queryable database and is optimized for computers." ;
  rdfs:subClassOf obo:ECO_0007647 .

obo:ECO_0007771 a rdfs:Class ;
  rdfs:label "deacetylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of deacetylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001548 , obo:ECO_0007307 .

obo:OBI_0002439 a rdfs:Class ;
  rdfs:label "chromosome conformation capture assay" ;
  rdfs:comment "A nuclear ligation assay that detects chromosomal interactions between any two genomic loci. Chromatin segments are cross-linked, cut by restriction enzymes, ligated, and finally analyzed by PCR." ;
  rdfs:subClassOf obo:OBI_0002438 .

obo:GO_1902679 a rdfs:Class ;
  rdfs:label "negative regulation of RNA biosynthetic process" ;
  rdfs:subClassOf obo:GO_0010558 , obo:GO_0031327 , obo:GO_0051253 , obo:GO_0065007 , obo:GO_2001141 .

obo:ECO_0008010 a rdfs:Class ;
  rdfs:label "support vector machine evidence" ;
  rdfs:comment "A type of machine learning method evidence that uses a supervised learning model with associated algorithms for classification. Support vector machines aim to determine a hyperplane that separates two classes while maximizing the distance from the hyperplane to any given data point in those classes." ;
  rdfs:subClassOf obo:ECO_0008000 .

obo:GO_0050896 a rdfs:Class ;
  rdfs:label "response to stimulus" ;
  rdfs:comment "Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus. The process begins with detection of the stimulus and ends with a change in state or activity or the cell or organism." ;
  rdfs:subClassOf obo:GO_0008150 .

obo:ECO_0008164 a rdfs:Class ;
  rdfs:label "antibody Y3JP-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody Y3JP-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008077 , obo:ECO_0008088 .

obo:ECO_0008041 a rdfs:Class ;
  rdfs:label "antibody 14.4.4-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody 14.4.4 to determine that the H2-IAg7 protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0000032 a rdfs:Class ;
  rdfs:label "nucleotide BLAST evidence used in manual assertion" ;
  rdfs:comment "A type of nucleotide BLAST evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000030 , obo:ECO_0000207 .

obo:ECO_0000078 a rdfs:Class ;
  rdfs:label "southern hybridization evidence" ;
  rdfs:comment "A type of DNA detection assay evidence that is based on detection of a specific DNA sequence by hybridization of labeled probes to any immobilized DNA fragment with sequence similarity." ;
  rdfs:subClassOf obo:ECO_0005519 .

obo:ECO_0001187 a rdfs:Class ;
  rdfs:label "histology evidence used in manual assertion" ;
  rdfs:comment "A type of histology evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001051 , obo:ECO_0001256 .

obo:ECO_0008038 a rdfs:Class ;
  rdfs:label "curator inference based on intrinsic disorder prediction used in automatic assertion" ;
  rdfs:comment "A type of curator inference based on intrinsic disorder prediction that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000213 , obo:ECO_0007322 , obo:ECO_0008032 .

obo:ECO_0000004 a rdfs:Class ;
  rdfs:label "cell fractionation evidence" ;
  rdfs:comment "A type of fractionation evidence where sub-cellular components are separated based on their physical properties such as density in a sucrose density gradient." ;
  rdfs:subClassOf obo:ECO_0000100 .

obo:ECO_0006049 a rdfs:Class ;
  rdfs:label "genetic transformation evidence used in manual assertion" ;
  rdfs:comment "A type of genetic transformation evidence that is used in a manual assertion" ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0005027 .

obo:ECO_0001570 a rdfs:Class ;
  rdfs:label "single-cell RNA-sequencing evidence used in manual assertion" ;
  rdfs:comment "A type of single cell RNA-sequencing evidence that is used in manual assertion." ;
  rdfs:subClassOf obo:ECO_0001560 , obo:ECO_0006068 .

obo:ECO_0005671 a rdfs:Class ;
  rdfs:label "x-ray crystallography evidence used in automatic assertion" ;
  rdfs:comment "A type of x-ray crystallography evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001823 , obo:ECO_0007374 .

obo:GO_1905643 a rdfs:Class ;
  rdfs:label "positive regulation of DNA methylation" ;
  rdfs:subClassOf obo:GO_0044030 , obo:GO_0051054 , obo:GO_0065007 .

obo:ECO_0008139 a rdfs:Class ;
  rdfs:label "antibody LB3.1-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody LB3.1-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008065 , obo:ECO_0008087 .

obo:OBI_0002625 a rdfs:Class ;
  rdfs:label "gene knockdown" ;
  rdfs:comment "A genetic transformation which reduces the expression of a specific gene or genes in an organism." ;
  rdfs:subClassOf obo:OBI_0600043 .

obo:ECO_0007056 a rdfs:Class ;
  rdfs:label "differential interference contrast microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of differential interference contrast microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001232 , obo:ECO_0007055 .

obo:UO_0000002 a rdfs:Class ;
  rdfs:label "mass unit" ;
  rdfs:comment "A unit which is a standard measure of the amount of matter/energy of a physical object." ;
  rdfs:subClassOf obo:IAO_0000003 .

obo:GO_0051716 a rdfs:Class ;
  rdfs:label "cellular response to stimulus" ;
  rdfs:subClassOf obo:GO_0009987 , obo:GO_0050896 .

obo:ECO_0006190 a rdfs:Class ;
  rdfs:label "cryogenic electron microscopy-based structural model with high relative B-factor values" ;
  rdfs:comment "A type of cryogenic electron microscopy evidence in which a structural model is built that contains high relative B-factor values indicating flexibility in that area of the molecule." ;
  rdfs:subClassOf obo:ECO_0006181 .

obo:ECO_0006005 a rdfs:Class ;
  rdfs:label "fractionation evidence used in manual assertion" ;
  rdfs:comment "A type of fractionation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000100 , obo:ECO_0000314 .

obo:GO_0004536 a rdfs:Class ;
  rdfs:label "deoxyribonuclease activity" ;
  rdfs:subClassOf obo:GO_0004518 , obo:GO_0140097 .

obo:ECO_0008175 a rdfs:Class ;
  rdfs:label "T cell epitope assay type based MHC restriction evidence used in manual assertion" ;
  rdfs:comment "A type of T cell epitope assay type based MHC restriction evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0008083 .

obo:ECO_0008100 a rdfs:Class ;
  rdfs:label "antibody 34-7-23S-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody 34-7-23S-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008045 , obo:ECO_0008088 .

obo:ECO_0001553 a rdfs:Class ;
  rdfs:label "farnesylation assay evidence" ;
  rdfs:comment "A type of direct assay evidence where farnesylated residues in proteins are detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007748 a rdfs:Class ;
  rdfs:label "phenotypic similarity evidence used in manual assertion" ;
  rdfs:comment "A type of phenotypic similarity evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000057 , obo:ECO_0000252 .

obo:OBI_0000456 a rdfs:Class ;
  rdfs:label "material transformation objective"@en ;
  rdfs:comment "an objective specifiction that creates an specific output object from input materials."@en ;
  rdfs:subClassOf obo:IAO_0000005 .

obo:ECO_0007640 a rdfs:Class ;
  rdfs:label "curator inference from MedlinePlus encyclopedia" ;
  rdfs:comment "A type of curator inference from encyclopedia in which the reference is to an article in the National Library of Medicine's MedLinePlus encyclopedia." ;
  rdfs:subClassOf obo:ECO_0007637 .

obo:ECO_0000101 a rdfs:Class ;
  rdfs:label "Affymetrix GeneChip evidence" ;
  rdfs:comment "A type of cRNA to DNA expression microarray evidence resulting from the use of a proprietary Affymetrix GeneChip System for analyzing complex genetic information." ;
  rdfs:subClassOf obo:ECO_0000062 .

obo:ECO_0007356 a rdfs:Class ;
  rdfs:label "cell counting evidence used in automatic assertion" ;
  rdfs:comment "A type of cell counting evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001020 , obo:ECO_0007773 .

obo:GO_0061045 a rdfs:Class ;
  rdfs:label "negative regulation of wound healing" ;
  rdfs:subClassOf obo:GO_0032102 , obo:GO_0061041 , obo:GO_0065007 , obo:GO_1903035 .

obo:GO_0043388 a rdfs:Class ;
  rdfs:label "positive regulation of DNA binding" ;
  rdfs:subClassOf obo:GO_0051099 , obo:GO_0051101 , obo:GO_0065007 .

obo:ECO_0007754 a rdfs:Class ;
  rdfs:label "curator inference from Wikipedia used in manual assertion" ;
  rdfs:comment "A type of curator inference from Wikipedia that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007638 , obo:ECO_0007753 .

obo:ECO_0008070 a rdfs:Class ;
  rdfs:label "antibody OX18-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody OX18 to determine that the rat MHC class I protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0008182 a rdfs:Class ;
  rdfs:label "T cell type based MHC restriction evidence used in automatic assertion" ;
  rdfs:comment "A type of T cell type based MHC restriction evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007307 , obo:ECO_0008086 .

obo:OBI_0600005 a rdfs:Class ;
  rdfs:label "collecting specimen from organism"@en ;
  rdfs:comment "a process with the objective to obtain a material entity that was part of an organism for potential future use in an investigation"@en ;
  rdfs:subClassOf obo:OBI_0000659 .

obo:GO_0045892 a rdfs:Class ;
  rdfs:label "negative regulation of transcription, DNA-templated" ;
  rdfs:subClassOf obo:GO_0006355 , obo:GO_0065007 , obo:GO_1903507 .

obo:OBI_0002090 a rdfs:Class ;
  rdfs:label "rapid amplification of cDNA ends"@en ;
  rdfs:comment "A reverse transcribed polymerase chain reaction that is used to amplify a mRNA sequence between a defined internal site and the 5' or 3' end of the mRNA."@en ;
  rdfs:subClassOf obo:OBI_0000552 .

obo:ECO_0006183 a rdfs:Class ;
  rdfs:label "particle scattering evidence" ;
  rdfs:comment "A type of structure determination evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight, diffusion and interaction strength by irradiating the sample with a particle beam and the scattered intensity is probed at a certain angle by a detector." ;
  rdfs:subClassOf obo:ECO_0005031 .

obo:OBI_0002189 a rdfs:Class ;
  rdfs:label "local field potential recording" ;
  rdfs:comment "An extracellular electrophysiology assay where a microelectrode is placed in the extracellular space of brain tissue to measure action potential and compared to an electrode either outside or inside that tissue." ;
  rdfs:subClassOf obo:OBI_0000454 .

obo:OBI_0001139 a rdfs:Class ;
  rdfs:label "liquid chromatography instrument" ;
  rdfs:comment "A chromatography device that dissolves a mixture in liquid mobile phase to separate the analyte to be measured from other molecules in the mixture and allows it to be isolated" ;
  rdfs:subClassOf obo:OBI_0000048 .

obo:ECO_0001075 a rdfs:Class ;
  rdfs:label "in vivo protein cleavage assay evidence" ;
  rdfs:comment "A type of protein cleavage assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001547 .

obo:ECO_0007575 a rdfs:Class ;
  rdfs:label "Northwestern blot evidence used in automatic assertion" ;
  rdfs:comment "A type of Northwestern blot evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000146 , obo:ECO_0007505 .

obo:OBI_0001977 a rdfs:Class ;
  rdfs:label "cytometry assay" ;
  rdfs:comment "An assay that counts and/or measures characteristics of cells." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:GO_0040008 a rdfs:Class ;
  rdfs:label "regulation of growth" ;
  rdfs:subClassOf obo:GO_0050789 , obo:GO_0065007 .

obo:ECO_0007173 a rdfs:Class ;
  rdfs:label "use dependence of inactivation evidence used in manual assertion" ;
  rdfs:comment "A type of use dependence of inactivation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005570 , obo:ECO_0006006 .

obo:OBI_1000048 a rdfs:Class ;
  rdfs:label "transgenic organism"@en ;
  rdfs:comment "a transgenic organism is material entity which derives from an organism which has been modified to express a gene not normally part of it"@en ;
  rdfs:subClassOf obo:OBI_0302859 .

obo:ECO_0001057 a rdfs:Class ;
  rdfs:label "in vitro acetylation assay evidence" ;
  rdfs:comment "A type of acetylation assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001546 .

obo:ECO_0006368 a rdfs:Class ;
  rdfs:label "computational structure modeling evidence used in manual assertion" ;
  rdfs:comment "A type of computational structure modeling evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0006341 , obo:ECO_0007668 .

obo:ECO_0007590 a rdfs:Class ;
  rdfs:label "high throughput expression pattern evidence used in automatic assertion" ;
  rdfs:comment "A type of high throughput expression pattern evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006057 , obo:ECO_0007006 , obo:ECO_0007298 .

obo:OBI_0000025 a rdfs:Class ;
  rdfs:label "reference substance role"@en ;
  rdfs:comment "a role inhering in a material entity that is realized when  characteristics or responses elicited by the substance are used for comparison or reference."@en ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:ECO_0001080 a rdfs:Class ;
  rdfs:label "in vivo desumoylation assay evidence" ;
  rdfs:comment "A type of desumoylation assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001551 .

obo:OBI_0000257 a rdfs:Class ;
  rdfs:label "DNA extraction"@en ;
  rdfs:comment "A DNA extraction is a nucleic acid extraction where the desired output material is DNA."@en ;
  rdfs:subClassOf obo:OBI_0666667 .

obo:ECO_0001801 a rdfs:Class ;
  rdfs:label "alkaline phosphatase reporter gene assay evidence" ;
  rdfs:comment "A type of reporter gene assay evidence based on the fusion of selected genes with the phoA gene to express alkaline phosphatase in periplasmic space for protein tracing." ;
  rdfs:subClassOf obo:ECO_0000049 .

obo:ECO_0001267 a rdfs:Class ;
  rdfs:label "tissue microarray evidence used in manual assertion" ;
  rdfs:comment "A type of tissue microarray evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001130 , obo:ECO_0001172 .

obo:ECO_0006300 a rdfs:Class ;
  rdfs:label "Raman spectroscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of Raman spectroscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006259 , obo:ECO_0007458 , obo:ECO_0007815 .

obo:GO_0003676 a rdfs:Class ;
  rdfs:label "nucleic acid binding" ;
  rdfs:subClassOf obo:GO_0097159 , obo:GO_1901363 .

obo:ECO_0000235 a rdfs:Class ;
  rdfs:label "5C evidence" ;
  rdfs:comment "A type of chromosome conformation-based evidence that is derived by performing ligation-mediated amplification (LMA) prior to product quantitation." ;
  rdfs:subClassOf obo:ECO_0000232 .

obo:ECO_0007560 a rdfs:Class ;
  rdfs:label "immunofluorescence wide-field microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of immunofluorescence wide-field microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005599 , obo:ECO_0007497 , obo:ECO_0007561 .

obo:ECO_0000271 a rdfs:Class ;
  rdfs:label "obsolete array experiment evidence used in manual assertion" ;
  rdfs:comment "This general 'array experiment' term should not be used - replace with the specific type of array (e.g. ECO:0000273, ECO:0000276, ECO:0000285, etc.)" .

obo:OBI_0400079 a rdfs:Class ;
  rdfs:label "optical filter" ;
  rdfs:comment "An optical filter is an optical subsystem that selectively transmits light having certain properties (often, a particular range of wavelengths, that is, range of colours of light), while blocking the remainder. They are commonly used in photography, in many optical instruments, and to colour stage lighting Optical filters can be arranged to segregate and collect light by wave length." ;
  rdfs:subClassOf obo:OBI_0000932 .

obo:ECO_0000352 a rdfs:Class ;
  rdfs:label "evidence used in manual assertion" ;
  rdfs:comment "A type of evidence that is used in an manual assertion." ;
  rdfs:subClassOf obo:ECO_0000000 .

obo:ECO_0001254 a rdfs:Class ;
  rdfs:label "radioisotope assay evidence used in manual assertion" ;
  rdfs:comment "A type of radioisotope assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001120 .

obo:OBI_0001266 a rdfs:Class ;
  rdfs:label "DNA methylation profiling by high throughput sequencing assay" ;
  rdfs:comment "A DNA methylation profiling assay in which the methylation state of DNA is determined and can be compared between samples using sequencing based technology" ;
  rdfs:subClassOf obo:OBI_0000634 .

obo:OBI_0000533 a rdfs:Class ;
  rdfs:label "affinity column" ;
  rdfs:comment "An affinity column is a chromatography column that is used in affinity chromatography. Differences in the affinity of molecules to be separated to a stationary phase are used for discriminate retention." ;
  rdfs:subClassOf obo:OBI_0000038 .

obo:ECO_0007016 a rdfs:Class ;
  rdfs:label "voltammetry evidence used in manual assertion" ;
  rdfs:comment "A type of voltammetry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0007015 .

obo:OBI_0000652 a rdfs:Class ;
  rdfs:label "material combination" ;
  rdfs:comment "is a material processing with the objective to combine two or more material entities as input into a single material entity as output." ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0007237 a rdfs:Class ;
  rdfs:label "3C-qPCR evidence used in automatic assertion" ;
  rdfs:comment "A type of 3C-qPCR evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000237 , obo:ECO_0007823 .

obo:ECO_0001169 a rdfs:Class ;
  rdfs:label "constitutively active mutant protein evidence used in manual assertion" ;
  rdfs:comment "A type of constitutively active mutant protein evidence that is used a in manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0001032 .

obo:ECO_0008009 a rdfs:Class ;
  rdfs:label "single molecule fluorescence in situ hybridization evidence" ;
  rdfs:comment "A type of fluorescence in situ hybridization evidence resulting from the use of fluorescent probes to localize and quantify individual RNA molecules in single cells for studying gene expression." ;
  rdfs:subClassOf obo:ECO_0001047 , obo:ECO_0005518 .

obo:GO_0065007 a rdfs:Class ;
  rdfs:label "biological regulation" ;
  rdfs:subClassOf obo:GO_0008150 .

obo:ECO_0007496 a rdfs:Class ;
  rdfs:label "immunofluorescence confocal microscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of immunofluorescence confocal microscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005600 , obo:ECO_0007490 , obo:ECO_0007497 .

obo:GO_0032879 a rdfs:Class ;
  rdfs:label "regulation of localization" ;
  rdfs:subClassOf obo:GO_0050789 , obo:GO_0065007 .

obo:ECO_0000246 a rdfs:Class ;
  rdfs:label "obsolete computational combinatorial evidence used in automatic assertion" ;
  rdfs:comment "OBSOLETE. A type of computational combinatorial analysis that is used in an automatic assertion." .

obo:ECO_0006365 a rdfs:Class ;
  rdfs:label "stopped-flow fluorescence spectroscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of stopped-flow fluorescence spectroscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006337 , obo:ECO_0007332 , obo:ECO_0007450 .

obo:ECO_0007702 a rdfs:Class ;
  rdfs:label "cytotoxicity assay evidence used in manual assertion" ;
  rdfs:comment "A type of cytotoxicity assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005012 , obo:ECO_0007695 .

obo:OBI_0000361 a rdfs:Class ;
  rdfs:label "ion source" ;
  rdfs:comment "An ion source is a device that is part of a mass spectrometer that ionizes the material under analysis. The ions are then transported by magnetic or electric fields to the mass analyzer.  Techniques for ionization have been key to determining what types of samples can be analyzed by mass spectrometry. Electron ionization and chemical ionization are used for gases and vapors. In chemical ionization sources, the material is ionized by chemical ion-molecule reactions during collisions in the source. Two techniques often used with liquid and solid biological samples include electrospray ionization (due to John Fenn PMID 2675315.) and matrix-assisted laser desorption/ionization (MALDI, due to M. Karas and F. Hillenkamp (Measuring Mass: From Positive Rays to Proteins by Michael A. Grayson (Editor) (ISBN 0-941901-31-9)))." ;
  rdfs:subClassOf obo:OBI_0000968 .

obo:ECO_0007597 a rdfs:Class ;
  rdfs:label "slide agglutination test evidence used in automatic assertion" ;
  rdfs:comment "A type of slide agglutination test evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007021 , obo:ECO_0007596 .

obo:ECO_0007849 a rdfs:Class ;
  rdfs:label "fluorescence quantification assay evidence used in manual assertion" ;
  rdfs:comment "A type of fluorescence quantification assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001249 , obo:ECO_0007847 .

obo:GO_0019904 a rdfs:Class ;
  rdfs:label "protein domain specific binding" ;
  rdfs:comment "Interacting selectively and non-covalently with a specific domain of a protein." ;
  rdfs:subClassOf obo:GO_0005515 , obo:OBI_0001588 .

obo:GO_0051099 a rdfs:Class ;
  rdfs:label "positive regulation of binding" ;
  rdfs:subClassOf obo:GO_0044093 , obo:GO_0051098 , obo:GO_0065007 .

obo:OBI_0000623 a rdfs:Class ;
  rdfs:label "NMR spectroscopy assay" ;
  rdfs:comment "An assay that exploits the magnetic properties of certain nuclei (those with a spin) to resonate when placed in particular magnetic field conditions. Instruments recording NMR spectrum and sets of analysis can be used to deduce identity of chemical as well as composition of complex chemical mixtures." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:GO_1901362 a rdfs:Class ;
  rdfs:label "organic cyclic compound biosynthetic process" ;
  rdfs:subClassOf obo:GO_1901360 , obo:GO_1901576 .

obo:GO_0051276 a rdfs:Class ;
  rdfs:label "chromosome organization" ;
  rdfs:comment "A process that is carried out at the cellular level that results in the assembly, arrangement of constituent parts, or disassembly of chromosomes, structures composed of a very long molecule of DNA and associated proteins that carries hereditary information. This term covers covalent modifications at the molecular level as well as spatial relationships among the major components of a chromosome." ;
  rdfs:subClassOf obo:GO_0006996 .

obo:ECO_0000260 a rdfs:Class ;
  rdfs:label "match to InterPro member signature evidence used in manual assertion" ;
  rdfs:comment "A type of match to InterPro member signature evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000029 , obo:ECO_0000255 .

obo:ECO_0006165 a rdfs:Class ;
  rdfs:label "nuclear magnetic resonance spectroscopy evidence used in manual assertion" ;
  rdfs:comment "A type of nuclear magnetic resonance spectroscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001238 , obo:ECO_0006163 .

obo:ECO_0006380 a rdfs:Class ;
  rdfs:label "asexual sporulation assay evidence used in manual assertion" ;
  rdfs:comment "A type of asexual sporulation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006345 , obo:ECO_0006378 .

obo:ECO_0000201 a rdfs:Class ;
  rdfs:label "sequence orthology evidence" ;
  rdfs:comment "A type of sequence similarity evidence in which orthology is established by multiple criteria, including amino acid and/or nucleotide sequence comparisons and one or more of the following: phylogenetic analysis, coincident expression, conserved map location, functional complementation, immunological cross-reaction, similarity in subcellular localization, subunit structure, substrate specificity, and response to specific inhibitors." ;
  rdfs:subClassOf obo:ECO_0000044 .

obo:ECO_0005008 a rdfs:Class ;
  rdfs:label "DNA synthesis cell proliferation assay evidence" ;
  rdfs:comment "A type of cell proliferation assay evidence resulting from the measure of DNA synthesis in the presece of a label." ;
  rdfs:subClassOf obo:ECO_0005007 .

obo:ECO_0007543 a rdfs:Class ;
  rdfs:label "air-liquid interface assay evidence used in automatic assertion" ;
  rdfs:comment "A type of air-liquid interface assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006024 , obo:ECO_0007541 .

obo:GO_0051051 a rdfs:Class ;
  rdfs:label "negative regulation of transport" ;
  rdfs:subClassOf obo:GO_0048519 , obo:GO_0051049 , obo:GO_0065007 .

obo:ECO_0007829 a rdfs:Class ;
  rdfs:label "combinatorial computational and experimental evidence used in automatic assertion" ;
  rdfs:comment "A type of combinatorial computational and experimental evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000213 , obo:ECO_0007661 .

obo:GO_0001508 a rdfs:Class ;
  rdfs:label "action potential" ;
  rdfs:comment "A process in which membrane potential cycles through a depolarizing spike, triggered in response to depolarization above some threshold, followed by repolarization. This cycle is driven by the flow of ions through various voltage gated channels with different thresholds and ion specificities." ;
  rdfs:subClassOf obo:GO_0042391 .

obo:GO_0032989 a rdfs:Class ;
  rdfs:label "cellular component morphogenesis" ;
  rdfs:subClassOf obo:GO_0009653 , obo:GO_0016043 , obo:GO_0048869 .

obo:ECO_0007367 a rdfs:Class ;
  rdfs:label "conditional knockin evidence used in automatic assertion" ;
  rdfs:comment "A type of conditional knockin evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001031 , obo:ECO_0007368 .

obo:GO_0043065 a rdfs:Class ;
  rdfs:label "positive regulation of apoptotic process" ;
  rdfs:subClassOf obo:GO_0042981 , obo:GO_0043068 , obo:GO_0065007 .

obo:GO_0032720 a rdfs:Class ;
  rdfs:label "negative regulation of tumor necrosis factor production" ;
  rdfs:subClassOf obo:GO_0032680 , obo:GO_0065007 , obo:GO_1903556 .

obo:GO_0001558 a rdfs:Class ;
  rdfs:label "regulation of cell growth" ;
  rdfs:subClassOf obo:GO_0040008 , obo:GO_0051128 , obo:GO_0065007 .

obo:GO_0030031 a rdfs:Class ;
  rdfs:label "cell projection assembly" ;
  rdfs:subClassOf obo:GO_0022607 , obo:GO_0030030 .

obo:GO_0044238 a rdfs:Class ;
  rdfs:label "primary metabolic process" ;
  rdfs:subClassOf obo:GO_0008152 .

obo:ECO_0007510 a rdfs:Class ;
  rdfs:label "systematic evolution of ligands by exponential amplification evidence used in automatic assertion" ;
  rdfs:comment "A type of systematic evolution of ligands by exponential amplification evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000293 , obo:ECO_0007332 .

obo:ECO_0000011 a rdfs:Class ;
  rdfs:label "genetic interaction evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence resulting from the effect that a given gene has on another gene or genes, and the products." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0007380 a rdfs:Class ;
  rdfs:label "DNA laddering assay evidence used in automatic assertion" ;
  rdfs:comment "A type of DNA laddering assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001039 , obo:ECO_0007777 .

obo:ECO_0007147 a rdfs:Class ;
  rdfs:label "competitive growth assay evidence used in manual assertion" ;
  rdfs:comment "A type of competitive growth assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000336 , obo:ECO_0007696 .

obo:ECO_0007376 a rdfs:Class ;
  rdfs:label "histochemistry evidence used in automatic assertion" ;
  rdfs:comment "A type of histochemistry evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001050 , obo:ECO_0007389 .

obo:OBI_0000319 a rdfs:Class ;
  rdfs:label "material to be added role"@en ;
  rdfs:comment "material to be added role is a protocol participant role realized by a material which is added into a material bearing the target of material addition role in a material addition process"@en ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:ECO_0007229 a rdfs:Class ;
  rdfs:label "Illumina sequencing evidence used in automatic assertion" ;
  rdfs:comment "A type of Illumina sequencing evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000222 , obo:ECO_0007814 .

obo:GO_0004519 a rdfs:Class ;
  rdfs:label "endonuclease activity" ;
  rdfs:subClassOf obo:GO_0004518 .

obo:GO_0031341 a rdfs:Class ;
  rdfs:label "regulation of cell killing" ;
  rdfs:subClassOf obo:GO_0050794 , obo:GO_0065007 .

obo:OBI_0001311 a rdfs:Class ;
  rdfs:label "X-ray crystallography assay determining the 3D structure of a T cell epitope:MHC:TCR complex" ;
  rdfs:comment "A X-ray crystallography 3D molecular structure determination assay that characterizes the 3-dimensional molecular structure of a T cell epitope:MHC:TCR complex." ;
  rdfs:subClassOf obo:OBI_0000912 , obo:OBI_0001999 .

obo:GO_0051100 a rdfs:Class ;
  rdfs:label "negative regulation of binding" ;
  rdfs:subClassOf obo:GO_0044092 , obo:GO_0051098 , obo:GO_0065007 .

obo:GO_0051224 a rdfs:Class ;
  rdfs:label "negative regulation of protein transport" ;
  rdfs:subClassOf obo:GO_0051051 , obo:GO_0051223 , obo:GO_0065007 , obo:GO_1904950 .

obo:GO_0006996 a rdfs:Class ;
  rdfs:label "organelle organization" ;
  rdfs:subClassOf obo:GO_0016043 .

obo:ECO_0006176 a rdfs:Class ;
  rdfs:label "proton-based nuclear magnetic resonance evidence" ;
  rdfs:comment "A type of nuclear magnetic resonance spectroscopy evidence in which the electromagnetic singal being measured comes from the perturbation of hydrogen nuclei resulting in a one dimensional spectrum." ;
  rdfs:subClassOf obo:ECO_0006163 .

obo:GO_2001021 a rdfs:Class ;
  rdfs:label "negative regulation of response to DNA damage stimulus" ;
  rdfs:subClassOf obo:GO_0048523 , obo:GO_0048585 , obo:GO_0065007 , obo:GO_2001020 .

obo:ECO_0001164 a rdfs:Class ;
  rdfs:label "co-sedimentation assay evidence used in manual assertion" ;
  rdfs:comment "A type of co-sedimentation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000353 , obo:ECO_0001028 .

obo:ECO_0007298 a rdfs:Class ;
  rdfs:label "expression pattern evidence used in automatic assertion" ;
  rdfs:comment "A type of expression pattern evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000008 , obo:ECO_0007297 .

obo:ECO_0005501 a rdfs:Class ;
  rdfs:label "in vivo polyADP-ribosylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vivo polyADP-ribosylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0005500 .

obo:ECO_0005657 a rdfs:Class ;
  rdfs:label "primer extension assay evidence used in manual assertion" ;
  rdfs:comment "A type of primer extension assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000291 , obo:ECO_0001819 .

obo:ECO_0007568 a rdfs:Class ;
  rdfs:label "yeast one-hybrid evidence used in automatic assertion" ;
  rdfs:comment "A type of yeast one-hybrid evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000066 , obo:ECO_0007432 .

obo:GO_0010556 a rdfs:Class ;
  rdfs:label "regulation of macromolecule biosynthetic process" ;
  rdfs:subClassOf obo:GO_0009889 , obo:GO_0060255 , obo:GO_0065007 .

obo:ECO_0001240 a rdfs:Class ;
  rdfs:label "nuclease protection assay evidence used in manual assertion" ;
  rdfs:comment "A type of nuclease protection assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001106 , obo:ECO_0007739 .

obo:ECO_0000083 a rdfs:Class ;
  rdfs:label "transmembrane domain prediction evidence" ;
  rdfs:comment "A type of motif similarity evidence that is based on detection of one, or more, transmembrane domains in the primary sequence of a protein through computational prediction and/or a manual examination of the sequence." ;
  rdfs:subClassOf obo:ECO_0000028 .

obo:GO_0009893 a rdfs:Class ;
  rdfs:label "positive regulation of metabolic process" ;
  rdfs:subClassOf obo:GO_0019222 , obo:GO_0048518 , obo:GO_0065007 .

obo:CHEBI_4705 a rdfs:Class ;
  rdfs:label "double-stranded DNA" ;
  rdfs:subClassOf obo:CHEBI_16991 .

obo:ECO_0006053 a rdfs:Class ;
  rdfs:label "pharmacological assay evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence that arises from assaying the response of a cell, tissue, organ or organism following exposure to a receptor agonist or antagonist." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0000022 a rdfs:Class ;
  rdfs:label "co-purification evidence" ;
  rdfs:comment "A type of physical interaction evidence where a cellular component subunit is isolated as part of purification of its larger complex." ;
  rdfs:subClassOf obo:ECO_0000021 .

obo:GO_0034645 a rdfs:Class ;
  rdfs:label "cellular macromolecule biosynthetic process" ;
  rdfs:subClassOf obo:GO_0009059 , obo:GO_0044249 , obo:GO_0044260 .

obo:ECO_0007760 a rdfs:Class ;
  rdfs:label "curator inference from dictionary used in manual assertion" ;
  rdfs:comment "A type of curator inference from dictionary that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007641 , obo:ECO_0007752 .

obo:ECO_0001575 a rdfs:Class ;
  rdfs:label "membrane fusion assay evidence used in automatic assertion" ;
  rdfs:comment "A type of membrane fusion assay evidence that is used in automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001574 , obo:ECO_0007307 .

obo:GO_0034764 a rdfs:Class ;
  rdfs:label "positive regulation of transmembrane transport" ;
  rdfs:subClassOf obo:GO_0034762 , obo:GO_0048522 , obo:GO_0051050 , obo:GO_0065007 .

obo:ECO_0007416 a rdfs:Class ;
  rdfs:label "in vivo deubiquitination assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vivo deubiquitination assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001079 , obo:ECO_0007791 .

obo:ECO_0007669 a rdfs:Class ;
  rdfs:label "computational evidence used in automatic assertion" ;
  rdfs:comment "A type of computational evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000501 , obo:ECO_0007672 .

obo:BFO_0000031 a rdfs:Class ;
  rdfs:label "generically dependent continuant"@en ;
  rdfs:comment "A continuant that is dependent on one or other independent continuant bearers. For every instance of A requires some instance of (an independent continuant type) B but which instance of B serves can change from time to time."@en ;
  rdfs:subClassOf obo:BFO_0000002 .

obo:ECO_0000339 a rdfs:Class ;
  rdfs:label "two-dimensional agarose gel electrophoresis evidence" ;
  rdfs:comment "A type of gel electrophoresis evidence where DNA molecules are electrophoresed on a low percentage agarose gel followed by high voltage electrophoresis on a higher percentage agarose gel in the presence of ethidium bromide." ;
  rdfs:subClassOf obo:ECO_0000337 .

obo:OBI_0000067 a rdfs:Class ;
  rdfs:label "evaluant role"@en ;
  rdfs:comment "a role that inheres in a material entity that is realized in an assay in which data is generated about the bearer of the evaluant role"@en ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:ECO_0008193 a rdfs:Class ;
  rdfs:label "avidity evidence used in automatic assertion" ;
  rdfs:comment "A type of avidity evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007332 , obo:ECO_0008185 .

obo:ECO_0000089 a rdfs:Class ;
  rdfs:label "restriction landmark genomic scanning evidence" ;
  rdfs:comment "A type of DNA detection assay evidence resulting from the use of restriction enzymes for direct end-labeling of DNA (creating landmarks), followed by high-resolution two-dimensional electrophoresis to visualize the landmarks." ;
  rdfs:subClassOf obo:ECO_0005519 .

obo:ECO_0001198 a rdfs:Class ;
  rdfs:label "in vitro methylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro methylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001065 .

obo:ECO_0007340 a rdfs:Class ;
  rdfs:label "adhesion assay evidence used in automatic assertion" ;
  rdfs:comment "A type of adhesion assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001006 , obo:ECO_0007773 .

obo:ECO_0007826 a rdfs:Class ;
  rdfs:label "phylogenetic evidence used in automatic assertion" ;
  rdfs:comment "A type of phylogenetic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000080 , obo:ECO_0000251 .

obo:ECO_0008027 a rdfs:Class ;
  rdfs:label "coordinate mapping to geographic property shapefiles used in automatic assertion" ;
  rdfs:comment "A type of coordinate mapping to geographic property shapefiles that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000363 , obo:ECO_0008014 .

obo:ECO_0007104 a rdfs:Class ;
  rdfs:label "co-fractionation evidence used in manual assertion" ;
  rdfs:comment "A type of co-fractionation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000102 , obo:ECO_0006005 .

obo:ECO_0001206 a rdfs:Class ;
  rdfs:label "in vitro ubiquitination assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro ubiquitination assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001073 .

obo:ECO_0007579 a rdfs:Class ;
  rdfs:label "transgenic rescue experiment evidence used in automatic assertion" ;
  rdfs:comment "A type of transgenic rescue experiment evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000013 , obo:ECO_0007578 .

obo:ECO_0007218 a rdfs:Class ;
  rdfs:label "heterologous system uptake evidence used in automatic assertion" ;
  rdfs:comment "A type of heterologous system uptake evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000162 , obo:ECO_0007789 .

obo:ECO_0001119 a rdfs:Class ;
  rdfs:label "quantitative mass spectrometry evidence" ;
  rdfs:comment "A type of mass spectrometry evidence resulting from quantitative analysis of peptides, proteins, and proteomes." ;
  rdfs:subClassOf obo:ECO_0001096 .

obo:ECO_0006101 a rdfs:Class ;
  rdfs:label "recessive mutant phenotype evidence used in manual assertion" ;
  rdfs:comment "A type of mutant phenotype evidence that is used in a manual assertion based on the analysis of a mutation which, in diploid organisms, must be present in both alleles for a phenotype to manifest itself." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0006100 .

obo:ECO_0001251 a rdfs:Class ;
  rdfs:label "protein microarray evidence used in manual assertion" ;
  rdfs:comment "A type of protein microarray evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0001117 .

obo:OBI_0000650 a rdfs:Class ;
  rdfs:label "differential expression analysis data transformation"@en ;
  rdfs:comment "A differential expression analysis data transformation is a data transformation that has an objective of differential expression analysis.  Frequently this data transformation involves summarizing or otherwise aggregating signals from various samples categorized into groups, and calculating measures of group differences from these aggregated signals."@en ;
  rdfs:subClassOf obo:OBI_0200000 .

obo:ECO_0005542 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence by experimental evidence from single species used in manual assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence by experimental evidence from single species that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005553 , obo:ECO_0007747 .

obo:ECO_0001086 a rdfs:Class ;
  rdfs:label "in vivo sumoylation assay evidence" ;
  rdfs:comment "A type of sumoylation assay evidence used in an in vivo experiment." ;
  rdfs:subClassOf obo:ECO_0001559 .

obo:OBI_0100066 a rdfs:Class ;
  rdfs:label "organ section"@en ;
  rdfs:comment "A processed material which derives from an organ and results from a process of dissection or histological sample preparation a portion(formerly an organ section is portion of an organ removed from the context of the organ)"@en ;
  rdfs:subClassOf obo:OBI_0000047 .

obo:ECO_0005575 a rdfs:Class ;
  rdfs:label "reconstituted bilayer single-channel patch recording evidence" ;
  rdfs:comment "A type of patch-clamp reocrding evidence that involves ion channel analysis by incorporating the ion channels into liposomes suitable for patch-clamping which allows isolation and study of a specific population of channels into that bilayer membrane." ;
  rdfs:subClassOf obo:ECO_0006012 .

obo:ECO_0006229 a rdfs:Class ;
  rdfs:label "Fourier-transform infrared spectroscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of Fourier-transform infrared spectroscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006191 , obo:ECO_0007458 .

obo:ECO_0006307 a rdfs:Class ;
  rdfs:label "turbidity measurement evidence used in manual assertion" ;
  rdfs:comment "A type of turbidity measurement evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0006263 .

obo:ECO_0005014 a rdfs:Class ;
  rdfs:label "obsolete in vitro cell based assay evidence" ;
  rdfs:comment "Use children of ECO:0001565 (cell-based assay evidence) in place of this term." .

obo:ECO_0006280 a rdfs:Class ;
  rdfs:label "bait-prey protein pull-down evidence used in automatic assertion" ;
  rdfs:comment "A type of bait-prey protein pull-down evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006249 , obo:ECO_0006393 .

obo:ECO_0000358 a rdfs:Class ;
  rdfs:label "differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact)" ;
  rdfs:comment "A type of transcript expression evidence resulting from a microarray analysis with the use of the Gene Set Enrichment Analysis (GSEA) method or the Fisher's-Exact (FE) method to assess the differential expression." ;
  rdfs:subClassOf obo:ECO_0000009 .

obo:ECO_0008017 a rdfs:Class ;
  rdfs:label "single molecule fluorescence in situ hybridization evidence used in automatic assertion" ;
  rdfs:comment "A type of single molecule fluorescence in situ hybridization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007386 , obo:ECO_0007818 , obo:ECO_0008009 .

obo:OBI_0000854 a rdfs:Class ;
  rdfs:label "western blot assay" ;
  rdfs:comment "An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with_ antibodies specific to the analyte molecules." ;
  rdfs:subClassOf obo:OBI_0001978 .

obo:ECO_0008111 a rdfs:Class ;
  rdfs:label "antibody B8.11.2-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody B8.11.2-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008051 , obo:ECO_0008087 .

<https://w3id.org/linkml/Any> a rdfs:Class ;
  rdfs:comment "An unconstrained type or object" .

obo:ECO_0007672 a rdfs:Class ;
  rdfs:label "computational evidence" ;
  rdfs:comment "A type of evidence in which data are produced, and/or generated, and/or analyzed on a computer." ;
  rdfs:subClassOf obo:ECO_0000000 .

obo:GO_0060355 a rdfs:Class ;
  rdfs:label "positive regulation of cell adhesion molecule production" ;
  rdfs:subClassOf obo:GO_0048522 , obo:GO_0060353 , obo:GO_0065007 .

obo:GO_1904950 a rdfs:Class ;
  rdfs:label "negative regulation of establishment of protein localization" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_0070201 , obo:GO_1903828 .

obo:ECO_0008136 a rdfs:Class ;
  rdfs:label "antibody IVD12-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody IVD12-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008063 , obo:ECO_0008088 .

obo:ECO_0008179 a rdfs:Class ;
  rdfs:label "specific MHC molecule based MHC restriction evidence used in manual assertion" ;
  rdfs:comment "A type of specific MHC molecule based MHC restriction evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0008085 .

obo:ECO_0005570 a rdfs:Class ;
  rdfs:label "use dependence of inactivation evidence" ;
  rdfs:comment "A type of electrophysiology assay evidence in which the amount of current remaining after a series of depolarization at different frequencies described as the normalized current related to the time course." ;
  rdfs:subClassOf obo:ECO_0000164 .

obo:ECO_0007122 a rdfs:Class ;
  rdfs:label "in vitro assay evidence used in manual assertion" ;
  rdfs:comment "A type of in vitro assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000181 , obo:ECO_0000314 .

obo:ECO_0001574 a rdfs:Class ;
  rdfs:label "membrane fusion assay evidence" ;
  rdfs:comment "A type of direct assay evidence in which a broad variety of physiological processes are measured when two separate membranes merge into a single contiguous membrane." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0005527 a rdfs:Class ;
  rdfs:label "matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion" ;
  rdfs:comment "A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is  used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000269 , obo:ECO_0005526 .

obo:ECO_0007843 a rdfs:Class ;
  rdfs:label "curator inference from journal publication used in automatic assertion" ;
  rdfs:comment "A type of curator inference from journal publication that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007645 , obo:ECO_0007833 .

obo:GO_0080135 a rdfs:Class ;
  rdfs:label "regulation of cellular response to stress" ;
  rdfs:subClassOf obo:GO_0050794 , obo:GO_0065007 , obo:GO_0080134 .

obo:ECO_0008085 a rdfs:Class ;
  rdfs:label "specific MHC molecule based MHC restriction evidence" ;
  rdfs:comment "A type of direct assay evidence that employs the use of a specific MHC molecule in a T cell response assay to determine that a specific ligand is restricted by that MHC molecule." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:IAO_0000005 a rdfs:Class ;
  rdfs:label "objective specification"@en ;
  rdfs:comment "A directive information entity that describes an intended process endpoint. When part of a plan specification the concretization is realized in a planned process in which the bearer tries to effect the world so that the process endpoint is achieved."@en ;
  rdfs:subClassOf obo:IAO_0000033 .

obo:GO_2000112 a rdfs:Class ;
  rdfs:label "regulation of cellular macromolecule biosynthetic process" ;
  rdfs:subClassOf obo:GO_0010556 , obo:GO_0031326 , obo:GO_0065007 .

obo:ECO_0006194 a rdfs:Class ;
  rdfs:label "selective antibody-based structural conformation evidence" ;
  rdfs:comment "A type of immunodetection assay evidence that involves the use of antibodies that are selective to conformations of the epitope to which they bind in order to provide information on conformations present in a target molecule." ;
  rdfs:subClassOf obo:ECO_0005031 , obo:ECO_0005593 .

obo:OBI_0002436 a rdfs:Class ;
  rdfs:label "RNA interactome capture" ;
  rdfs:comment "An assay that identifies RNA binding proteins by cross-linking RNA and proteins with UV light, then purifying the bound complexes by oligo(dT) capture. Finally, the complexes are analyzed by mass spectrometry." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:GO_0016070 a rdfs:Class ;
  rdfs:label "RNA metabolic process" ;
  rdfs:subClassOf obo:GO_0090304 .

obo:ECO_0000047 a rdfs:Class ;
  rdfs:label "spatial pattern of transcript expression evidence" ;
  rdfs:comment "A type of transcript expression evidence that accounts for the position of transcription ranging in scale from the position of genes on the chromosome to differing tissues to regions of an anatomical structure." ;
  rdfs:subClassOf obo:ECO_0000009 .

obo:ECO_0006291 a rdfs:Class ;
  rdfs:label "deglycosylation assay evidence used in manual assertion" ;
  rdfs:comment "A type of deglycosylation assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0006255 .

obo:ECO_0007250 a rdfs:Class ;
  rdfs:label "particle size and count assay evidence used in automatic assertion" ;
  rdfs:comment "A type of particle size and count assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000334 , obo:ECO_0007307 .

obo:ECO_0000118 a rdfs:Class ;
  rdfs:label "subtractive hybridization evidence" ;
  rdfs:comment "A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue are compared to another cell type or tissue when denatured ds-cDNA from the two samples are hybridized, and the common sequences are 'subtracted'." ;
  rdfs:subClassOf obo:ECO_0000009 , obo:IAO_0000030 .

obo:ECO_0001153 a rdfs:Class ;
  rdfs:label "blood test evidence used in manual assertion" ;
  rdfs:comment "A type of blood test evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001016 .

obo:ECO_0006075 a rdfs:Class ;
  rdfs:label "affinity evidence used in manual assertion" ;
  rdfs:comment "A type of physical interaction evidence that is used in manual assertion that depends on the strength of the interaction between two entities." ;
  rdfs:subClassOf obo:ECO_0000023 , obo:ECO_0000353 .

obo:ECO_0006188 a rdfs:Class ;
  rdfs:label "X-ray crystallography-based structural model with high relative B-factor values" ;
  rdfs:comment "A type of X-ray cystallography evidence in which a structural model is built that contains high relative B-factor values indicating flexibility in that area of the molecule." ;
  rdfs:subClassOf obo:ECO_0001823 .

obo:ECO_0006409 a rdfs:Class ;
  rdfs:label "combinatorial intrinsic disorder prediction and experimental evidence used in automatic assertion" ;
  rdfs:comment "A type of combinatorial intrinsic disorder prediction and experimental evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006408 , obo:ECO_0007829 .

obo:OBI_0200000 a rdfs:Class ;
  rdfs:label "data transformation"@en ;
  rdfs:comment "A planned process that produces output data from input data."@en ;
  rdfs:subClassOf obo:OBI_0000011 .

obo:ECO_0007832 a rdfs:Class ;
  rdfs:label "inferential evidence used in automatic assertion" ;
  rdfs:comment "A type of inferential evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000361 , obo:ECO_0000501 .

obo:OBI_0000811 a rdfs:Class ;
  rdfs:label "primary structure of DNA macromolecule" ;
  rdfs:comment "a quality of a DNA molecule that inheres in its bearer due to the order of its DNA nucleotide residues." ;
  rdfs:subClassOf obo:SO_0000001 .

obo:GO_0031324 a rdfs:Class ;
  rdfs:label "negative regulation of cellular metabolic process" ;
  rdfs:subClassOf obo:GO_0009892 , obo:GO_0031323 , obo:GO_0048523 , obo:GO_0065007 .

obo:OBI_0001505 a rdfs:Class ;
  rdfs:label "purification"@en ;
  rdfs:comment "A planned process to separate a material entity into different compositions of which one or more have are purified fractions that contain higher concentration of a desired component, while others contain impurities and are not of interest" ;
  rdfs:subClassOf obo:OBI_0600014 .

obo:ECO_0007207 a rdfs:Class ;
  rdfs:label "epitope-tagged protein immunolocalization evidence used in automatic assertion" ;
  rdfs:comment "A type of epitope-tagged protein immunolocalization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000092 , obo:ECO_0007486 .

obo:ECO_0001549 a rdfs:Class ;
  rdfs:label "defarnesylation assay evidence" ;
  rdfs:comment "A type of direct assay evidence where the removal of farnesyl groups from a protein is detected." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0001155 a rdfs:Class ;
  rdfs:label "bromodeoxyuridine incorporation assay evidence used in manual assertion" ;
  rdfs:comment "A type of bromodeoxyuridine incorporation assay evidence used in manual assertion." ;
  rdfs:subClassOf obo:ECO_0001018 , obo:ECO_0001241 .

obo:ECO_0007384 a rdfs:Class ;
  rdfs:label "affinity evidence used in automatic assertion" ;
  rdfs:comment "A type of affinity evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000023 , obo:ECO_0007332 .

obo:ECO_0006269 a rdfs:Class ;
  rdfs:label "cell aggregation evidence" ;
  rdfs:comment "A type of cell-based assay evidence resulting from the analysis of cells irreversibly binding to each other." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:ECO_0007795 a rdfs:Class ;
  rdfs:label "demethylation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of demethylation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001550 , obo:ECO_0007307 .

obo:ECO_0007647 a rdfs:Class ;
  rdfs:label "curator inference from authoritative source" ;
  rdfs:comment "A type of curator inference that is from what is generally considered an authoritative source on the topic, including model organism databases, newspaper articles, books, journal publications, etc." ;
  rdfs:subClassOf obo:ECO_0000205 .

obo:GO_0007000 a rdfs:Class ;
  rdfs:label "nucleolus organization" ;
  rdfs:subClassOf obo:GO_0006997 .

obo:ECO_0000328 a rdfs:Class ;
  rdfs:label "coding sequence splice pattern evidence" ;
  rdfs:comment "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript." ;
  rdfs:subClassOf obo:ECO_0000326 .

obo:OBI_0002744 a rdfs:Class ;
  rdfs:label "molecular label"@en ;
  rdfs:comment "A molecular reagent intended to associate with some molecular target to serve as a proxy for the presence, abundance, or location of this target in a detection of molecular label assay"@en ;
  rdfs:subClassOf obo:CHEBI_23367 , obo:OBI_0001879 .

obo:ECO_0006346 a rdfs:Class ;
  rdfs:label "sexual sporulation assay evidence" ;
  rdfs:comment "A type of sporulation assay evidence that reflects the presence, absence, and/or amount of sexual spore formation." ;
  rdfs:subClassOf obo:ECO_0006344 .

obo:ECO_0007752 a rdfs:Class ;
  rdfs:label "curator inference from authoritative source used in manual assertion" ;
  rdfs:comment "A type of curator inference from authoritative source that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000305 , obo:ECO_0007647 .

obo:ECO_0006379 a rdfs:Class ;
  rdfs:label "sporulation assay evidence used in automatic assertion" ;
  rdfs:comment "A type of sporulation assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006344 , obo:ECO_0007307 .

obo:ECO_0006408 a rdfs:Class ;
  rdfs:label "combinatorial intrinsic disorder prediction and experimental evidence" ;
  rdfs:comment "A type of combinatorial computational and experimental evidence in which intrinsic disorder prediction evidence and experimental evidence have been used in combination in order to establish the basis for an assertion." ;
  rdfs:subClassOf obo:ECO_0007661 .

obo:ECO_0006398 a rdfs:Class ;
  rdfs:label "antibody Tu36-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody Tu36 to determine that the HLA-DR protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0006064 a rdfs:Class ;
  rdfs:label "cell-free assay evidence used in manual assertion" ;
  rdfs:comment "A type of cell-free assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000183 , obo:ECO_0000314 .

obo:ECO_0000134 a rdfs:Class ;
  rdfs:label "transport assay evidence" ;
  rdfs:comment "A type of direct assay evidence resulting from the activity assessment of transport proteins." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007463 a rdfs:Class ;
  rdfs:label "terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in automatic assertion" ;
  rdfs:comment "A type of terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001133 , obo:ECO_0007777 .

obo:ECO_0000227 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation-PCR evidence" ;
  rdfs:comment "A type of chromatin immunoprecipitation evidence that uses polymerase chain reaction (PCR) where specific primers are used on the pulled-down DNA for DNA detection." ;
  rdfs:subClassOf obo:ECO_0000226 .

obo:ECO_0007513 a rdfs:Class ;
  rdfs:label "heteronuclear single quantum coherence spectroscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of heteronuclear single quantum coherence spectroscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001813 , obo:ECO_0006164 .

obo:ECO_0008074 a rdfs:Class ;
  rdfs:label "antibody Tu39-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody Tu39 to determine that the human MHC class II protein complex presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:GO_0022603 a rdfs:Class ;
  rdfs:label "regulation of anatomical structure morphogenesis" ;
  rdfs:subClassOf obo:GO_0050793 , obo:GO_0065007 .

obo:OBI_0000800 a rdfs:Class ;
  rdfs:label "cross linking" ;
  rdfs:comment "A process in which bonds are created that link one polymer to another" ;
  rdfs:subClassOf obo:OBI_0000094 .

obo:ECO_0007452 a rdfs:Class ;
  rdfs:label "protein microarray evidence used in automatic assertion" ;
  rdfs:comment "A type of protein microarray evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001117 , obo:ECO_0007819 .

obo:OBI_0000964 a rdfs:Class ;
  rdfs:label "in live cell assay" ;
  rdfs:comment "An assay in which a measurement is made by observing entities located in a live cell." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:OBI_0000659 a rdfs:Class ;
  rdfs:label "specimen collection process" ;
  rdfs:comment "A planned process with the objective of collecting a specimen." ;
  rdfs:subClassOf obo:OBI_0000011 .

obo:GO_0005575 a rdfs:Class ;
  rdfs:label "cellular_component" ;
  rdfs:comment "A location, relative to cellular compartments and structures, occupied by a macromolecular machine when it carries out a molecular function. There are two ways in which the gene ontology describes locations of gene products: (1) relative to cellular structures (e.g., cytoplasmic side of plasma membrane) or compartments (e.g., mitochondrion), and (2) the stable macromolecular complexes of which they are parts (e.g., the ribosome)." ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:GO_1903829 a rdfs:Class ;
  rdfs:label "positive regulation of protein localization" ;
  rdfs:subClassOf obo:GO_0032880 , obo:GO_0048518 , obo:GO_0065007 .

obo:ECO_0005667 a rdfs:Class ;
  rdfs:label "site-directed mutagenesis phenotypic evidence used in manual assertion" ;
  rdfs:comment "A type of site-directed phenotypic mutagenesis evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000315 , obo:ECO_0005528 .

obo:ECO_0001271 a rdfs:Class ;
  rdfs:label "resonant mirror biosensor evidence used in manual assertion" ;
  rdfs:comment "A type of resonant mirror biosensor evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001123 .

obo:ECO_0007782 a rdfs:Class ;
  rdfs:label "protein cleavage assay evidence used in automatic assertion" ;
  rdfs:comment "A type of protein cleavage assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001547 , obo:ECO_0007307 .

obo:GO_0006260 a rdfs:Class ;
  rdfs:label "DNA replication" ;
  rdfs:subClassOf obo:GO_0034645 .

obo:ECO_0005538 a rdfs:Class ;
  rdfs:label "obsolete computationally derived logical inference" .

obo:ECO_0007804 a rdfs:Class ;
  rdfs:label "in vitro transcription reconstitution assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro transcription reconstitution assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000150 , obo:ECO_0007803 .

obo:ECO_0000313 a rdfs:Class ;
  rdfs:label "imported information used in automatic assertion" ;
  rdfs:comment "A type of imported information that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000311 , obo:ECO_0006156 .

obo:ECO_0000268 a rdfs:Class ;
  rdfs:label "flow cytometry evidence" ;
  rdfs:comment "A type of direct assay evidence in which cells or particles are characterized by passing them through a light source and monitoring the resultant light scatter or fluorochrome excitation." ;
  rdfs:subClassOf obo:ECO_0000002 .

obo:ECO_0007196 a rdfs:Class ;
  rdfs:label "gain-of-function mutant phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of gain-of-function mutant phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000042 , obo:ECO_0007325 .

obo:ECO_0007492 a rdfs:Class ;
  rdfs:label "immunogold labelling electron microscopy assay evidence used in automatic assertion" ;
  rdfs:comment "A type of immunogold labelling electron microscopy assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005591 , obo:ECO_0007485 , obo:ECO_0007493 .

obo:ECO_0000130 a rdfs:Class ;
  rdfs:label "beta-glucuronidase fusion protein localization evidence" ;
  rdfs:comment "A type of fusion protein localization evidence resulting from the targeted insertion of the beta-glucuronidase (GUS) coding gene as a reporter gene for the localization of a particular gene product." ;
  rdfs:subClassOf obo:ECO_0000124 .

obo:ECO_0000105 a rdfs:Class ;
  rdfs:label "Nimblegen array evidence" ;
  rdfs:comment "A type of cDNA to DNA expression microarray evidence resulting from the use of a proprietary NimbleGen array to measure expression levels." ;
  rdfs:subClassOf obo:ECO_0000097 .

obo:ECO_0006087 a rdfs:Class ;
  rdfs:label "Northwestern blot evidence used in manual assertion" ;
  rdfs:comment "A type of nucleic acid binding evidence that is used in a manual assertion in which RNA-protein binding is detected using labeled RNA as probes, hybridized to electrophoretically separated proteins." ;
  rdfs:subClassOf obo:ECO_0000146 , obo:ECO_0006083 .

obo:OBI_0002440 a rdfs:Class ;
  rdfs:label "Hi-C assay" ;
  rdfs:comment "A chromosome conformation capture assay that detects genome-wide chromosomal interactions. High-throughput techniques are used to sequence the ligated fragments after cross-linking and cutting with restriction enzymes." ;
  rdfs:subClassOf obo:OBI_0002439 .

obo:ECO_0008146 a rdfs:Class ;
  rdfs:label "antibody ME1-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody ME1-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008068 , obo:ECO_0008088 .

obo:OBI_0000892 a rdfs:Class ;
  rdfs:label "Southern blot assay" ;
  rdfs:comment "An analyte assay that detects the presence of a specific sequence in a DNA sample, which has been digested by restriction enzymes, resolved by gel electrophoresis, and blotted to a solid support, followed by hybridization of a probe raised against a specific sequence and detected with fluorescence or radioactivity." ;
  rdfs:subClassOf obo:OBI_0000443 .

obo:ECO_0000216 a rdfs:Class ;
  rdfs:label "phylogenetic determination of loss of key residues evidence" ;
  rdfs:comment "A type of phylogenetic evidence characterized by the absence of key sequence residues." ;
  rdfs:subClassOf obo:ECO_0000080 .

obo:ECO_0001052 a rdfs:Class ;
  rdfs:label "high-performance liquid chromatography evidence" ;
  rdfs:comment "A type of chromatography evidence resulting from the separation of molecules in a solute by forcing the solvent containing the sample through a column packed with nonporous particles at high pressure." ;
  rdfs:subClassOf obo:ECO_0000325 .

obo:ECO_0007302 a rdfs:Class ;
  rdfs:label "differential methylation hybridization evidence used in automatic assertion" ;
  rdfs:comment "A type of differential methylation hybridization evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000069 , obo:ECO_0007401 .

obo:ECO_0000208 a rdfs:Class ;
  rdfs:label "protein BLAST evidence" ;
  rdfs:comment "A type of BLAST evidence in which amino acid or translated nucleotide sequences are aligned." ;
  rdfs:subClassOf obo:ECO_0000206 .

obo:ECO_0006387 a rdfs:Class ;
  rdfs:label "host penetration assay evidence used in automatic assertion" ;
  rdfs:comment "A type of host penetration assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001831 , obo:ECO_0006348 .

obo:ECO_0007067 a rdfs:Class ;
  rdfs:label "colony papillation assay phenotypic evidence" ;
  rdfs:comment "A type of colony morphology phenotypic evidence resulting from the formation of papillae (microcolonies) that protrude outwards from the main colony by mutant cells." ;
  rdfs:subClassOf obo:ECO_0007077 .

obo:ECO_0006187 a rdfs:Class ;
  rdfs:label "X-ray crystallography-based structural model with missing residue coordinates" ;
  rdfs:comment "A type of X-ray cystallography evidence in which a structural model is built lacking coordinates for some residues indicating high flexibility or structural disorder in that area of the molecule." ;
  rdfs:subClassOf obo:ECO_0001823 .

obo:ECO_0000039 a rdfs:Class ;
  rdfs:label "obsolete protein assay evidence" ;
  rdfs:comment "OBSOLETE. A type of direct assay evidence resulting from determining the presence, abundance, structure, function, or activity of proteins." .

obo:ECO_0001039 a rdfs:Class ;
  rdfs:label "DNA laddering assay evidence" ;
  rdfs:comment "A type of apoptotic assay evidence resulting from the visualization via gel electrophoresis of the fragmented DNA (DNA ladder) which is the result of apoptotic DNA fragmentation." ;
  rdfs:subClassOf obo:ECO_0005034 .

obo:ECO_0007798 a rdfs:Class ;
  rdfs:label "staining evidence used in automatic assertion" ;
  rdfs:comment "A type of staining evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005019 , obo:ECO_0007307 .

obo:ECO_0005577 a rdfs:Class ;
  rdfs:label "electroencephalography recording evidence" ;
  rdfs:comment "A type of electrophysiology assay evidence that involves recording the electrical activity of the brain by means of electrodes placed on the surface of the head." ;
  rdfs:subClassOf obo:ECO_0000164 .

obo:OBI_0000902 a rdfs:Class ;
  rdfs:label "post mortem specimen" ;
  rdfs:comment "a specimen that was taken from a dead organism" ;
  rdfs:subClassOf obo:OBI_0001506 .

obo:GO_0032680 a rdfs:Class ;
  rdfs:label "regulation of tumor necrosis factor production" ;
  rdfs:subClassOf obo:GO_0065007 , obo:GO_1903555 .

obo:ECO_0006022 a rdfs:Class ;
  rdfs:label "microtiter plate biofilm assay evidence" ;
  rdfs:comment "A type of biofilm formation assay evidence in which microtiter dishes or tubes are inoculated and incubated to promote biofilm formation, and then biofilms are detected by staining with crystal violet or safranin to observe phenotypes." ;
  rdfs:subClassOf obo:ECO_0006020 .

obo:ECO_0008142 a rdfs:Class ;
  rdfs:label "antibody M1/42-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody M1/42-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008066 , obo:ECO_0008088 .

obo:OBI_0001396 a rdfs:Class ;
  rdfs:label "stimulus or stress design" ;
  rdfs:comment "A study design in which the response of an organism(s) to the stress or stimulus is studied, e.g. osmotic stress, heat shock, radiation exposure, behavioral treatment etc." ;
  rdfs:subClassOf obo:OBI_0000115 .

obo:ECO_0001028 a rdfs:Class ;
  rdfs:label "co-sedimentation assay evidence" ;
  rdfs:comment "A type of physical interaction evidence resulting from the separation of a mixture of molecules under the influence of a force such as artificial gravity, where molecules sedimenting together are assumed to interact." ;
  rdfs:subClassOf obo:ECO_0000021 .

obo:ECO_0007763 a rdfs:Class ;
  rdfs:label "curator inference from Merriam-Webster Dictionary used in manual assertion" ;
  rdfs:comment "A type of curator inference from Merriam-Webster Dictionary that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007643 , obo:ECO_0007760 .

obo:ECO_0006161 a rdfs:Class ;
  rdfs:label "medical practitioner statement evidence used in manual assertion" ;
  rdfs:comment "A type of medical practitioner statement evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006152 , obo:ECO_0006155 .

obo:ECO_0006098 a rdfs:Class ;
  rdfs:label "temperature-sensitive mutant phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence resulting from altered gene function at higher temperatures." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:GO_0006259 a rdfs:Class ;
  rdfs:label "DNA metabolic process" ;
  rdfs:subClassOf obo:GO_0044260 , obo:GO_0090304 .

obo:ECO_0007535 a rdfs:Class ;
  rdfs:label "electrophysiology assay evidence used in automatic assertion" ;
  rdfs:comment "A type of electrophysiology assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000164 , obo:ECO_0007307 .

obo:GO_0034250 a rdfs:Class ;
  rdfs:label "positive regulation of cellular amide metabolic process" ;
  rdfs:subClassOf obo:GO_0031325 , obo:GO_0034248 , obo:GO_0051173 , obo:GO_0065007 .

obo:ECO_0008096 a rdfs:Class ;
  rdfs:label "antibody 34-1-2S-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody 34-1-2S-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008043 , obo:ECO_0008088 .

obo:GO_0090238 a rdfs:Class ;
  rdfs:label "positive regulation of arachidonic acid secretion" ;
  rdfs:subClassOf obo:GO_0032305 , obo:GO_0065007 , obo:GO_0090237 .

obo:OBI_0001143 a rdfs:Class ;
  rdfs:label "labeled nucleic acid extract" ;
  rdfs:comment "a labeled specimen that is the output of a labeling process and has grain labeled nucleic acid for detection of the nucleic acid in future experiments." ;
  rdfs:subClassOf obo:OBI_0000924 .

obo:ECO_0000180 a rdfs:Class ;
  rdfs:label "clinical study evidence" ;
  rdfs:comment "A type of experimental evidence arising from a controlled investigation that uses human subjects." ;
  rdfs:subClassOf obo:BFO_0000002 , obo:ECO_0000006 .

obo:GO_0031329 a rdfs:Class ;
  rdfs:label "regulation of cellular catabolic process" ;
  rdfs:subClassOf obo:GO_0009894 , obo:GO_0031323 , obo:GO_0065007 .

obo:GO_2000279 a rdfs:Class ;
  rdfs:label "negative regulation of DNA biosynthetic process" ;
  rdfs:subClassOf obo:GO_0051053 , obo:GO_0065007 , obo:GO_2000113 , obo:GO_2000278 .

obo:ECO_0000166 a rdfs:Class ;
  rdfs:label "two-electrode voltage clamp recording evidence" ;
  rdfs:comment "A type of voltage clamp recording evidence which involves using two electrodes inserted into a large cell (often a xenopus oocyte) where one electrode is used to measure the internal potential (voltage) of the cell and the other electrode is used to clamp the current." ;
  rdfs:subClassOf obo:ECO_0005576 .

obo:ECO_0008125 a rdfs:Class ;
  rdfs:label "antibody HB145-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody HB145-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008058 , obo:ECO_0008087 .

obo:ECO_0000179 a rdfs:Class ;
  rdfs:label "animal model system study evidence" ;
  rdfs:comment "A type of experimental evidence arising from the investigation of an animal model system." ;
  rdfs:subClassOf obo:ECO_0000006 , obo:IAO_0000030 .

obo:ECO_0005613 a rdfs:Class ;
  rdfs:label "inference by association of genotype from phenotype" ;
  rdfs:comment "A type of natural variation mutant evidence used in manual assertion where alleles are directly linked to phenotype and Mendelian diseases as a result of various experiments." ;
  rdfs:subClassOf obo:ECO_0001237 .

obo:ECO_0007399 a rdfs:Class ;
  rdfs:label "in vitro deubiquitination assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro deubiquitination assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001059 , obo:ECO_0007791 .

obo:ECO_0001579 a rdfs:Class ;
  rdfs:label "spheroplast fusion assay evidence used in manual assertion" ;
  rdfs:comment "A type of spheroplast fusion assay evidence that is used in manual assertion." ;
  rdfs:subClassOf obo:ECO_0001576 , obo:ECO_0001577 .

obo:OBI_0001865 a rdfs:Class ;
  rdfs:label "assay array" ;
  rdfs:comment "A device made to be used in an analyte assay for immobilization of substances that bind the analyte at regular spatial positions on a surface." ;
  rdfs:subClassOf obo:OBI_0000968 .

obo:ECO_0007285 a rdfs:Class ;
  rdfs:label "cut-open oocyte voltage clamp recording evidence used in automatic assertion" ;
  rdfs:comment "A type of cut-open oocyte voltage clamp recording evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005583 , obo:ECO_0007766 .

obo:ECO_0007061 a rdfs:Class ;
  rdfs:label "light scattering evidence" ;
  rdfs:comment "A type of structure determination evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight, diffusion and interaction strength by illuminating the sample with a laser beam and the scattered intensity is probed at a certain angle by a detector." ;
  rdfs:subClassOf obo:ECO_0005031 .

obo:ECO_0008068 a rdfs:Class ;
  rdfs:label "antibody ME1-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody ME1 to determine that the HLA protein complex with B27 serotype presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:ECO_0006239 a rdfs:Class ;
  rdfs:label "polyadenylated transcript 3'-end-sequencing evidence" ;
  rdfs:comment "A type of RNA-sequencing evidence based on sequencing of the 3' ends of cDNA molecules that are selected by presence of a polyadenylated tail." ;
  rdfs:subClassOf obo:ECO_0000295 .

obo:ECO_0007373 a rdfs:Class ;
  rdfs:label "protein binding evidence used in automatic assertion" ;
  rdfs:comment "A type of protein binding evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000024 , obo:ECO_0007384 .

obo:ECO_0001828 a rdfs:Class ;
  rdfs:label "inference of sequence features from visual inspection used in manual assertion" ;
  rdfs:comment "A type of inference of sequence features from visual inspection that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0007751 , obo:ECO_0007850 .

obo:ECO_0007034 a rdfs:Class ;
  rdfs:label "neutralization test assay evidence used in manual assertion" ;
  rdfs:comment "A type of neutralization test assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006078 , obo:ECO_0007033 .

obo:ECO_0008122 a rdfs:Class ;
  rdfs:label "antibody DK1G8-specificity based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of antibody DK1G8-specificity based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008056 , obo:ECO_0008088 .

obo:GO_0001172 a rdfs:Class ;
  rdfs:label "transcription, RNA-templated" ;
  rdfs:comment "The cellular synthesis of RNA on a template of RNA." ;
  rdfs:subClassOf obo:GO_0009987 .

obo:IAO_0000027 a rdfs:Class ;
  rdfs:label "data item"@en ;
  rdfs:comment "An information content entity that is intended to be a truthful statement about something (modulo, e.g., measurement precision or other systematic errors) and is constructed/acquired by a method which reliably tends to produce (approximately) truthful statements."@en ;
  rdfs:subClassOf obo:IAO_0000030 .

obo:ECO_0006172 a rdfs:Class ;
  rdfs:label "mixed support of intron positions by RNA-sequencing alignment evidence" ;
  rdfs:comment "A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignments from a mixture of samples support all of the intron positions (exon pairs) predicted for a transcript." ;
  rdfs:subClassOf obo:ECO_0000342 .

obo:ECO_0008006 a rdfs:Class ;
  rdfs:label "deep learning neural network method evidence used in automatic assertion" ;
  rdfs:comment "A type of deep learning neural network method evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008001 , obo:ECO_0008025 .

obo:ECO_0007601 a rdfs:Class ;
  rdfs:label "viral hemagglutination inhibition assay evidence used in automatic assertion" ;
  rdfs:comment "A type of viral hemagglutination inhibition assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0007029 , obo:ECO_0007596 .

obo:GO_0060353 a rdfs:Class ;
  rdfs:label "regulation of cell adhesion molecule production" ;
  rdfs:subClassOf obo:GO_0050794 , obo:GO_0065007 .

obo:ECO_0008168 a rdfs:Class ;
  rdfs:label "specific MHC protein expression-based MHC presentation evidence used in automatic assertion" ;
  rdfs:comment "A type of specific MHC protein expression-based MHC presentation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0008079 , obo:ECO_0008166 .

obo:GO_0010468 a rdfs:Class ;
  rdfs:label "regulation of gene expression" ;
  rdfs:subClassOf obo:GO_0060255 , obo:GO_0065007 .

obo:GO_0090305 a rdfs:Class ;
  rdfs:label "nucleic acid phosphodiester bond hydrolysis" ;
  rdfs:subClassOf obo:GO_0090304 .

obo:OBI_0000975 a rdfs:Class ;
  rdfs:label "cell-cell binding detection by flow cytometry assay" ;
  rdfs:comment "A binding assay which uses a flow cytometer to detect pairs of cells that are bound to each other by staining them with different fluorescent labels." ;
  rdfs:subClassOf obo:OBI_0001977 .

obo:ECO_0007726 a rdfs:Class ;
  rdfs:label "fusion protein localization evidence used in manual assertion" ;
  rdfs:comment "A type of fusion protein localization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000124 , obo:ECO_0000314 .

obo:GO_0032101 a rdfs:Class ;
  rdfs:label "regulation of response to external stimulus" ;
  rdfs:subClassOf obo:GO_0048583 , obo:GO_0065007 .

obo:ECO_0007593 a rdfs:Class ;
  rdfs:label "combinatorial experimental and curator inference evidence used in automatic assertion" ;
  rdfs:comment "A type of combinatorial evidence from curator knowledge and experimental evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000213 , obo:ECO_0007012 .

obo:OBI_0000634 a rdfs:Class ;
  rdfs:label "DNA methylation profiling assay" ;
  rdfs:comment "An assay that measures the state of methylation of DNA molecules using genomic DNA collected from a material entity using a range of techniques and instrument such as DNA sequencers and often relying on treatment with bisulfites to ensure cytosine conversion." ;
  rdfs:subClassOf obo:OBI_0002020 .

obo:ECO_0007274 a rdfs:Class ;
  rdfs:label "macroscopic current trace evidence used in automatic assertion" ;
  rdfs:comment "A type of macroscopic current trace evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0005563 , obo:ECO_0007766 .

obo:ECO_0005555 a rdfs:Class ;
  rdfs:label "multiple sequence alignment evidence" ;
  rdfs:comment "A type of sequence alignment evidence where three or more biological sequences (protein or nucleic acid) of similar length are aligned to infer homology and the evolutionary relationships between the sequences studied." ;
  rdfs:subClassOf obo:ECO_0000200 .

obo:ECO_0007045 a rdfs:Class ;
  rdfs:label "scanning electron microscopy evidence" ;
  rdfs:comment "A type of electron microscopy evidence where a focused beam of high-energy electrons is used to generate a variety of signals at the surface of solid specimens." ;
  rdfs:subClassOf obo:ECO_0005033 .

obo:ECO_0000238 a rdfs:Class ;
  rdfs:label "Hi-C evidence" ;
  rdfs:comment "A type of chromosome conformation-based evidence that is derived by performing immunoprecipitation enrichment of the biotin labelled ligated fragments events prior to sequencing of the library and mapping it to the genome." ;
  rdfs:subClassOf obo:ECO_0000232 .

obo:GO_0002831 a rdfs:Class ;
  rdfs:label "regulation of response to biotic stimulus" ;
  rdfs:subClassOf obo:GO_0048583 , obo:GO_0065007 .

obo:ECO_0000205 a rdfs:Class ;
  rdfs:label "curator inference" ;
  rdfs:comment "A type of inferential evidence that is based on a conclusion drawn by a curator." ;
  rdfs:subClassOf obo:ECO_0000361 .

obo:ECO_0007261 a rdfs:Class ;
  rdfs:label "cell staining evidence used in automatic assertion" ;
  rdfs:comment "A type of cell staining evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001603 , obo:ECO_0007773 .

obo:ECO_0007191 a rdfs:Class ;
  rdfs:label "inference by association of genotype from phenotype used in manual assertion" ;
  rdfs:comment "A type of inference by association of genotype from phenotype that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001237 , obo:ECO_0005613 .

obo:GO_2000145 a rdfs:Class ;
  rdfs:label "regulation of cell motility" ;
  rdfs:subClassOf obo:GO_0040012 , obo:GO_0051270 , obo:GO_0065007 .

obo:ECO_0007151 a rdfs:Class ;
  rdfs:label "specific protein inhibition by antibody evidence used in manual assertion" ;
  rdfs:comment "A type of specific protein inhibition by antibody evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000341 , obo:ECO_0007707 .

obo:OBI_0002181 a rdfs:Class ;
  rdfs:label "outside-out patch clamp assay" ;
  rdfs:comment "A patch-clamp assay where a patch of the membrane is attached to the patch pipette. In this configuration, the external surface of the cell membrane is exposed as the outside of the membrane patch relative to the patch electrode. An outside-out patch starts with a gigaohm seal in a whole-cell recording configuration. The electrode is slowly withdrawn from the cell, until a fragment of membrane bulges away from the cell, which detaches and reforms as a convex membrane on the end of the electrode, with the original external surface of the membrane facing outward from the electrode. This provides the experimenter with access to the extracellular surface of the membrane via the bath and can manipulate the environment at the extracellular surface of single ion channels." ;
  rdfs:subClassOf obo:OBI_0002177 .

obo:ECO_0007430 a rdfs:Class ;
  rdfs:label "macroscopy evidence used in automatic assertion" ;
  rdfs:comment "A type of macroscopy evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001094 , obo:ECO_0007297 .

obo:ECO_0006042 a rdfs:Class ;
  rdfs:label "cell fractionation evidence used in manual assertion" ;
  rdfs:comment "A type of cell fractionation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000004 , obo:ECO_0006005 .

obo:OBI_0002076 a rdfs:Class ;
  rdfs:label "collection of specimens"@en ;
  rdfs:comment "A material entity that has two or more specimens as its parts."@en ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:RO_0002351 a rdf:Property ;
  rdfs:label "has member"@en ;
  rdfs:comment "has member is a mereological relation between a collection and an item." ;
  rdfs:range obo:OBI_0100051 ;
  rdfs:subPropertyOf obo:BFO_0000051 .

obo:OBI_0001358 a rdfs:Class ;
  rdfs:label "post-transcriptional modification design" ;
  rdfs:comment "A study design in which a modification of the transcriptome, proteome (not genome) is made, for example RNAi, antibody targeting." ;
  rdfs:subClassOf obo:OBI_0000115 .

obo:ECO_0007319 a rdfs:Class ;
  rdfs:label "author statement used in automatic assertion" ;
  rdfs:comment "A type of author statement that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000204 , obo:ECO_0006156 .

obo:ECO_0006199 a rdfs:Class ;
  rdfs:label "proton-based nuclear magnetic resonance evidence used in automatic assertion" ;
  rdfs:comment "A type of proton-based nuclear magnetic resonance evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006164 , obo:ECO_0006176 .

obo:ECO_0001568 a rdfs:Class ;
  rdfs:label "quantitative reverse transcription polymerase chain reaction evidence used in automatic assertion." ;
  rdfs:comment "A type of quantitative reverse transcription polymerase chain reaction evidence used in automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001566 , obo:ECO_0007475 .

obo:OBI_0000164 a rdfs:Class ;
  rdfs:label "clonal cDNA library"@en ;
  rdfs:comment """Mixed population of cDNAs (complementaryDNA) made from mRNA from a defined source, usually a specific cell type. This term should be associated only to nucleic acid interactors not to their proteins product. For instance in 2h screening use living cells (MI:0349) as sample process.

ALT DEF (PRS):: a cDNA library is a collection of host cells, typically E.Coli cells but not exclusively. modified by transfer of plasmid DNA molecule used as vector containing a fragment or totality of cDNA molecule (the insert) .  cDNA library may have an array of role and applications."""@en ;
  rdfs:subClassOf obo:OBI_0000731 .

obo:OBI_0000409 a rdfs:Class ;
  rdfs:label "nucleic acid template role"@en ;
  rdfs:comment "a reference substance role which inheres in nucleic acid material entity and is realized in the process of using the nucleic acid bearing the template role as a reference during synthesis of a reverse copy." ;
  rdfs:subClassOf obo:OBI_0000025 .

obo:ECO_0008133 a rdfs:Class ;
  rdfs:label "antibody ILA-88-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody ILA-88-specificity based MHC presentation evidence that is used in a manual assertion" ;
  rdfs:subClassOf obo:ECO_0008062 , obo:ECO_0008087 .

obo:OBI_1110120 a rdfs:Class ;
  rdfs:label "assay antigen role"@en ;
  rdfs:comment "Any molecule recognized by the adaptive immune receptors? Recognized means bound with a certain affinity? From GO ag binding:Interacting selectively with an antigen, any substance which is capable of inducing a specific immune response and of reacting with the products of that response, the specific antibody or specifically sensitized T-lymphocytes, or both. Binding may counteract the biological activity of the antigen. see OBI_1110120 below"@en ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:ECO_0001064 a rdfs:Class ;
  rdfs:label "in vitro farnesylation assay evidence" ;
  rdfs:comment "A type of farnesylation assay evidence used in an in vitro experiment." ;
  rdfs:subClassOf obo:ECO_0001553 .

obo:ECO_0007405 a rdfs:Class ;
  rdfs:label "in vitro protein kinase assay evidence used in automatic assertion" ;
  rdfs:comment "A type of in vitro protein kinase assay evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001068 , obo:ECO_0007767 .

obo:ECO_0007106 a rdfs:Class ;
  rdfs:label "green fluorescent protein fusion protein localization evidence used in manual assertion" ;
  rdfs:comment "A type of green fluorescent protein fusion protein localization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000126 , obo:ECO_0000314 .

obo:ECO_0001128 a rdfs:Class ;
  rdfs:label "syngeneic transplantation experiment evidence" ;
  rdfs:comment "A type of experimental phenotypic evidence resulting from a transplantation in which the transplanted material (stem cells) is from an individual's identical twin." ;
  rdfs:subClassOf obo:ECO_0000059 .

obo:ECO_0001183 a rdfs:Class ;
  rdfs:label "fluorescence resonance energy transfer evidence used in manual assertion" ;
  rdfs:comment "A type of fluorescence resonance energy transfer evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001048 , obo:ECO_0006327 .

obo:ECO_0006231 a rdfs:Class ;
  rdfs:label "heat capacity-based evidence used in automatic assertion" ;
  rdfs:comment "A type of heat capacity-based evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0006192 , obo:ECO_0007307 .

obo:ECO_0000278 a rdfs:Class ;
  rdfs:label "array-based sequence capture evidence used in manual assertion" ;
  rdfs:comment "A type of array-based sequence capture evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000093 , obo:ECO_0000269 .

obo:OBI_0002055 a rdfs:Class ;
  rdfs:label "assay measuring biological activity resulting from T cell epitope:MHC:TCR binding" ;
  rdfs:comment "A T cell epitope recognition assay that measures the immune response process resulting from the binding of a T cell receptor to epitope or the recongition of the epitope." ;
  rdfs:subClassOf obo:OBI_0000070 , obo:OBI_0003128 .

obo:ECO_0001177 a rdfs:Class ;
  rdfs:label "RNA dot blot assay evidence used in manual assertion" ;
  rdfs:comment "A type of RNA dot blot assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001040 , obo:ECO_0007739 .

obo:GO_0051171 a rdfs:Class ;
  rdfs:label "regulation of nitrogen compound metabolic process" ;
  rdfs:subClassOf obo:GO_0019222 , obo:GO_0065007 .

obo:ECO_0008157 a rdfs:Class ;
  rdfs:label "antibody Tu39-specificity based MHC presentation evidence used in manual assertion" ;
  rdfs:comment "A type of antibody Tu39-specificity based MHC presentation evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0008074 , obo:ECO_0008087 .

obo:ECO_0007175 a rdfs:Class ;
  rdfs:label "whole-cell voltage clamp recording evidence used in manual assertion" ;
  rdfs:comment "A type of whole-cell voltage clamp recording evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005572 , obo:ECO_0006006 .

obo:ECO_0007810 a rdfs:Class ;
  rdfs:label "anatomical perturbation phenotypic evidence used in automatic assertion" ;
  rdfs:comment "A type of anatomical perturbation phenotypic evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000240 , obo:ECO_0007635 .

obo:ECO_0007746 a rdfs:Class ;
  rdfs:label "biological system reconstruction evidence used in manual assertion" ;
  rdfs:comment "A type of biological system reconstruction evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000088 , obo:ECO_0000305 .

obo:GO_0032892 a rdfs:Class ;
  rdfs:label "positive regulation of organic acid transport" ;
  rdfs:subClassOf obo:GO_0032890 , obo:GO_0051050 , obo:GO_0065007 .

obo:OBI_0000097 a rdfs:Class ;
  rdfs:label "participant under investigation role"@en ;
  rdfs:comment "A role that is realized through the execution of a study design in which the bearer of the role participates and in which data about that bearer is collected." ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:ECO_0001139 a rdfs:Class ;
  rdfs:label "51Cr release assay evidence used in manual assertion" ;
  rdfs:comment "A type of 51Cr release assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001004 , obo:ECO_0007702 .

obo:ECO_0007233 a rdfs:Class ;
  rdfs:label "chromatin immunoprecipitation-qPCR evidence used in automatic assertion" ;
  rdfs:comment "A type of chromatin immunoprecipitation-qPCR evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000228 , obo:ECO_0007786 .

obo:OBI_1110034 a rdfs:Class ;
  rdfs:label "antigen"@en ;
  rdfs:comment "is a material entity that has the antigen role"@en ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:OBI_0001631 a rdfs:Class ;
  rdfs:label "electron microscopy imaging assay" ;
  rdfs:comment "An imaging assay in which an electrons are used to probe the density, shape and composition of an input material which are detected in an electron microscope and utilized to produce an image of the material." ;
  rdfs:subClassOf obo:OBI_0000185 , obo:OBI_0002119 .

obo:GO_0010941 a rdfs:Class ;
  rdfs:label "regulation of cell death" ;
  rdfs:subClassOf obo:GO_0050794 , obo:GO_0065007 .

obo:ECO_0007546 a rdfs:Class ;
  rdfs:label "co-immunoprecipitation evidence used in automatic assertion" ;
  rdfs:comment "A type of co-immunoprecipitation evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0000070 , obo:ECO_0007503 .

obo:OBI_0000913 a rdfs:Class ;
  rdfs:label "promoter activity detection by reporter gene assay" ;
  rdfs:comment "An assay in which the activity of a promoter in a cell is monitored by using a reporter gene that was inserted in a genomic location under control of the promoter and whose expression can be easily detected based on qualities or functions of the gene." ;
  rdfs:subClassOf obo:OBI_0000070 .

obo:ECO_0001563 a rdfs:Class ;
  rdfs:label "cell growth assay evidence" ;
  rdfs:comment "A type of direct assay evidence where biological cell development, i.e. an increase in cell mass and size are measured." ;
  rdfs:subClassOf obo:ECO_0001565 .

obo:ECO_0007143 a rdfs:Class ;
  rdfs:label "beta-galactosidase protein immunolocalization evidence used in manual assertion" ;
  rdfs:comment "A type of beta-galactosidase protein immunolocalization evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000301 , obo:ECO_0006031 .

obo:ECO_0001593 a rdfs:Class ;
  rdfs:label "polysome profiling evidence used in automatic assertion" ;
  rdfs:comment "A type of polysome profiling evidence that is used in an automatic assertion." ;
  rdfs:subClassOf obo:ECO_0001592 , obo:ECO_0007297 .

obo:ECO_0008063 a rdfs:Class ;
  rdfs:label "antibody IVD12-specificity based MHC presentation evidence" ;
  rdfs:comment "A type of antibody-specificity based MHC presentation evidence that employs antibody IVD12 to determine that HLA protein complex with DQ3 serotype presented a ligand." ;
  rdfs:subClassOf obo:ECO_0008039 .

obo:GO_0010927 a rdfs:Class ;
  rdfs:label "cellular component assembly involved in morphogenesis" ;
  rdfs:subClassOf obo:GO_0022607 , obo:GO_0048646 .

obo:ECO_0005512 a rdfs:Class ;
  rdfs:label "frameshift mutation phenotypic evidence" ;
  rdfs:comment "A type of mutant phenotype evidence resulting from any mutation that causes an insertion or deletion of nucleotides in a DNA sequence that is not divisible by three and therefore changes the codon reading frame and the translated amino acids." ;
  rdfs:subClassOf obo:ECO_0000015 .

obo:ECO_0000116 a rdfs:Class ;
  rdfs:label "differential hybridization evidence" ;
  rdfs:comment "A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue is compared to another cell type or tissue when mRNA from each sample is reverse transcribed, labeled, and hybridized to a cDNA library to compare hybridization patterns." ;
  rdfs:subClassOf obo:ECO_0000009 , obo:IAO_0000030 .

obo:ECO_0006220 a rdfs:Class ;
  rdfs:label "X-ray crystallography-based structural model with missing residue coordinates used in manual assertion" ;
  rdfs:comment "A type of X-ray crystallography-based structural model with missing residue coordinates that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0005670 , obo:ECO_0006187 .

obo:OBI_0000086 a rdfs:Class ;
  rdfs:label "reagent role"@en ;
  rdfs:comment "A role inhering in a biological or chemical entity that is intended to be applied in a scientific technique to participate (or have molecular components that participate) in a chemical reaction that facilitates the generation of data about some entity distinct from the bearer, or the generation of some specified material output distinct from the bearer."@en ;
  rdfs:subClassOf obo:BFO_0000023 .

obo:ECO_0001166 a rdfs:Class ;
  rdfs:label "comet assay evidence used in manual assertion" ;
  rdfs:comment "A type of comet assay evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0000314 , obo:ECO_0001029 .

obo:GO_0030097 a rdfs:Class ;
  rdfs:label "hemopoiesis" ;
  rdfs:comment "The process whose specific outcome is the progression of the myeloid and lymphoid derived organ/tissue systems of the blood and other parts of the body over time, from formation to the mature structure. The site of hemopoiesis is variable during development, but occurs primarily in bone marrow or kidney in many adult vertebrates." ;
  rdfs:subClassOf obo:GO_0008150 .

obo:ECO_0007020 a rdfs:Class ;
  rdfs:label "agglutination test evidence used in manual assertion" ;
  rdfs:comment "A type of agglutination test evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0006078 , obo:ECO_0007019 .

obo:OBI_0000048 a rdfs:Class ;
  rdfs:label "chromatography device" ;
  rdfs:comment "A device that facilitates the separation of mixtures. The function of a chromatography device involves passing a mixture dissolved in a \"mobile phase\" through a stationary phase, which separates the analyte to be measured from other molecules in the mixture and allows it to be isolated." ;
  rdfs:subClassOf obo:OBI_0000832 , obo:OBI_0000932 , obo:OBI_0002089 .

obo:OBI_0000403 a rdfs:Class ;
  rdfs:label "sort function"@en ;
  rdfs:comment "A sort function is a function to distinguish material components based on some associated physical quality or entity and to partition the separate components into distinct fractions according to a defined order."@en ;
  rdfs:subClassOf obo:OBI_0000372 .

obo:GO_0051241 a rdfs:Class ;
  rdfs:label "negative regulation of multicellular organismal process" ;
  rdfs:subClassOf obo:GO_0048519 , obo:GO_0051239 , obo:GO_0065007 .

obo:GO_0051641 a rdfs:Class ;
  rdfs:label "cellular localization" ;
  rdfs:subClassOf obo:GO_0009987 , obo:GO_0051179 .

obo:GO_0032056 a rdfs:Class ;
  rdfs:label "positive regulation of translation in response to stress" ;
  rdfs:subClassOf obo:GO_0043555 , obo:GO_0045727 , obo:GO_0065007 .

obo:OBI_0001225 a rdfs:Class ;
  rdfs:label "genetic population background information"@en ;
  rdfs:comment "a genetic characteristics information which is a part of genotype information that identifies the population of organisms"@en ;
  rdfs:subClassOf obo:OBI_0001404 .

obo:OBI_0400115 a rdfs:Class ;
  rdfs:label "spectrophotometer" ;
  rdfs:comment "A spectrophotometer is an instrument that measures the intensity of light as a function of the color, or more specifically, the wavelength of light, transmitted by a substance." ;
  rdfs:subClassOf obo:OBI_0000832 .

obo:ECO_0006145 a rdfs:Class ;
  rdfs:label "density functional theory simulation evidence used in manual assertion" ;
  rdfs:comment "A type of density functional theory simulation evidence that is used in manual assertion." ;
  rdfs:subClassOf obo:ECO_0006144 .

obo:OBI_1110045 a rdfs:Class ;
  rdfs:label "disposition to be bound by an adaptive immune receptor"@en ;
  rdfs:comment "Is the disposition borne by a material entity that is realized in a process of being bound by a adaptive immune receptor."@en ;
  rdfs:subClassOf obo:BFO_0000016 .

obo:ECO_0005586 a rdfs:Class ;
  rdfs:label "high throughput mass spectrometry evidence used in manual assertion" ;
  rdfs:comment "A type of high throughput mass spectrometry evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001230 , obo:ECO_0005585 .

obo:OBI_0000873 a rdfs:Class ;
  rdfs:label "pre-mortem specimen" ;
  rdfs:comment "a specimen that was taken from a live organism" ;
  rdfs:subClassOf obo:OBI_0001506 .

obo:ECO_0001839 a rdfs:Class ;
  rdfs:label "colorimetric in situ hybridization evidence" ;
  rdfs:comment "A type of nucleic acid localization evidence and in situ hybridization evidence resulting from the use of colorimetric probes to detect complementary sequences of nucleic acids." ;
  rdfs:subClassOf obo:ECO_0001836 .

obo:ECO_0000094 a rdfs:Class ;
  rdfs:label "obsolete biological assay evidence" ;
  rdfs:comment "The children of 'biological assay evidence' have been moved under 'direct assay evidence', and this term has been deprecated with no children left." .

obo:ECO_0007050 a rdfs:Class ;
  rdfs:label "phase contrast microscopy evidence used in manual assertion" ;
  rdfs:comment "A type of phase contrast microscopy evidence that is used in a manual assertion." ;
  rdfs:subClassOf obo:ECO_0001232 , obo:ECO_0007049 .

obo:ECO_9000001 a rdf:Property ;
  rdfs:label "uses" ;
  rdfs:comment "A relation that is an inverse of \"used_in\" relation." ;
  rdfs:range <https://w3id.org/linkml/Any> .

obo:ECO_9000002 a rdf:Property ;
  rdfs:label "has GO evidence code" ;
  rdfs:comment "A mapping of an evidence term to a Gene Ontology evidence code." ;
  rdfs:range xsd:string .

obo:IAO_0000112 a rdf:Property ;
  rdfs:label "example of usage" ;
  rdfs:comment "A phrase describing how a term should be used and/or a citation to a work which uses it. May also include other kinds of examples that facilitate immediate understanding, such as widely know prototypes or instances of a class, or cases where a relation is said to hold." ;
  rdfs:range xsd:string .

obo:IAO_0000115 a rdf:Property ;
  rdfs:label "definition" ;
  rdfs:comment "The official definition, explaining the meaning of a class or property. Shall be Aristotelian, formalized and normalized. Can be augmented with colloquial definitions." ;
  rdfs:range xsd:string .

obo:IAO_0000117 a rdf:Property ;
  rdfs:label "term editor" ;
  rdfs:comment "Name of editor entering the term in the file. The term editor is a point of contact for information regarding the term. The term editor may be, but is not always, the author of the definition, which may have been worked upon by several people" ;
  rdfs:range xsd:string .

obo:IAO_0000119 a rdf:Property ;
  rdfs:label "definition source" ;
  rdfs:comment "formal citation, e.g. identifier in external database to indicate / attribute source(s) for the definition. Free text indicate / attribute source(s) for the definition. EXAMPLE: Author Name, URI, MeSH Term C04, PUBMED ID, Wiki uri on 31.01.2007" ;
  rdfs:range xsd:string .

obo:IAO_0000234 a rdf:Property ;
  rdfs:label "ontology term requester" ;
  rdfs:comment "The 'term requester' can credit the person, organization or project who request the ontology term.,The name of the person, project, or organization that motivated inclusion of an ontology term by requesting its addition." ;
  rdfs:range xsd:string .

obo:IAO_0000417 a rdf:Property ;
  rdfs:label "is quality measured as"@en ;
  rdfs:comment "inverse of the relation of is quality measurement of"@en ;
  rdfs:range <https://w3id.org/linkml/Any> .

obo:OBI_0000295 a rdf:Property ;
  rdfs:label "is_specified_input_of"@en ;
  rdfs:comment "A relation between a planned process and a continuant participating in that process that is not created during  the process. The presence of the continuant during the process is explicitly specified in the plan specification which the process realizes the concretization of."@en ;
  rdfs:range obo:OBI_0000011 ;
  rdfs:subPropertyOf obo:RO_0000056 .

obo:RO_0000080 a rdf:Property ;
  rdfs:label "quality of"@en ;
  rdfs:comment "a relation between a quality and an independent continuant (the bearer), in which the quality specifically depends on the bearer for its existence"@en ;
  rdfs:range xsd:string ;
  rdfs:subPropertyOf obo:RO_0000052 .

obo:RO_0000086 a rdf:Property ;
  rdfs:label "has quality"@en ;
  rdfs:comment "a relation between an independent continuant (the bearer) and a quality, in which the quality specifically depends on the bearer for its existence"@en ;
  rdfs:range obo:BFO_0000019 ;
  rdfs:subPropertyOf obo:RO_0000053 .

obo:RO_0001001 a rdf:Property ;
  rdfs:label "derives into"@en ;
  rdfs:comment "a relation between two distinct material entities, the old entity and the new entity, in which the new entity begins to exist when the old entity ceases to exist, and the new entity inherits the significant portion of the matter of the old entity"@en ;
  rdfs:range <https://w3id.org/linkml/Any> .

obo:RO_0001015 a rdf:Property ;
  rdfs:label "location of"@en ;
  rdfs:comment "a relation between two independent continuants, the location and the target, in which the target is entirely within the location"@en ;
  rdfs:range <https://w3id.org/linkml/Any> .

obo:RO_0001025 a rdf:Property ;
  rdfs:label "located in"@en ;
  rdfs:comment "a relation between two independent continuants, the target and the location, in which the target is entirely within the location"@en ;
  rdfs:range <https://w3id.org/linkml/Any> .

obo:RO_0002087 a rdf:Property ;
  rdfs:label "immediately preceded by"@en ;
  rdfs:range xsd:string ;
  rdfs:subPropertyOf obo:BFO_0000062 .

obo:RO_0002090 a rdf:Property ;
  rdfs:label "immediately precedes"@en ;
  rdfs:range xsd:string ;
  rdfs:subPropertyOf obo:BFO_0000063 .

obo:RO_0002222 a rdf:Property ;
  rdfs:label "temporal relation"@en ;
  rdfs:range obo:BFO_0000003 .

obo:RO_0002350 a rdf:Property ;
  rdfs:label "member of"@en ;
  rdfs:comment "is member of is a mereological relation between a item and a collection." ;
  rdfs:range xsd:string ;
  rdfs:subPropertyOf obo:BFO_0000050 .

<http://purl.obolibrary.org/obo/eco#go_groupings> a rdf:Property ;
  rdfs:label "go_groupings" ;
  rdfs:comment "Grouping classes used by GO" ;
  rdfs:range xsd:string .

<http://purl.obolibrary.org/obo/eco#valid_with_biological_process> a rdf:Property ;
  rdfs:label "valid_with_biological_process" ;
  rdfs:comment "GO biological process terms should be used in the GO with/from field" ;
  rdfs:range xsd:string .

<http://purl.obolibrary.org/obo/eco#valid_with_cellular_component> a rdf:Property ;
  rdfs:label "valid_with_cellular_component" ;
  rdfs:comment "GO cellular component terms should be used in the GO with/from field" ;
  rdfs:range xsd:string .

<http://purl.obolibrary.org/obo/eco#valid_with_chemical_entity> a rdf:Property ;
  rdfs:label "valid_with_chemical_entity" ;
  rdfs:comment "Chemical entity IDs should be used in the GO with/from field" ;
  rdfs:range xsd:string .

<http://purl.obolibrary.org/obo/eco#valid_with_gene> a rdf:Property ;
  rdfs:label "valid_with_gene" ;
  rdfs:comment "Gene IDs should be used in the GO with/from field" ;
  rdfs:range xsd:string .

<http://purl.obolibrary.org/obo/eco#valid_with_molecular_function> a rdf:Property ;
  rdfs:label "valid_with_molecular_function" ;
  rdfs:comment "GO molecular function terms should be used in the GO with/from field" ;
  rdfs:range xsd:string .

<http://purl.obolibrary.org/obo/eco#valid_with_protein> a rdf:Property ;
  rdfs:label "valid_with_protein" ;
  rdfs:comment "Protein IDs should be used in the GO with/from field" ;
  rdfs:range xsd:string .

<http://purl.obolibrary.org/obo/eco#valid_with_protein_complex> a rdf:Property ;
  rdfs:label "valid_with_protein_complex" ;
  rdfs:comment "Protein complex IDs should be used in the GO with/from field" ;
  rdfs:range xsd:string .

<http://purl.obolibrary.org/obo/eco#valid_with_transcript> a rdf:Property ;
  rdfs:label "valid_with_transcript" ;
  rdfs:comment "Transcript IDs should be used in the GO with/from field" ;
  rdfs:range xsd:string .

<http://www.w3.org/2002/07/owl#real> a rdfs:Datatype .

obo:IAO_0000409 a rdfs:Class ;
  rdfs:label "denotator type"@en ;
  rdfs:comment "A denotator type indicates how a term should be interpreted from an ontological perspective."@en ;
  rdfs:subClassOf obo:IAO_0000102 .

obo:IAO_0000410 a obo:IAO_0000409 ;
  rdfs:label "universal"@en .

obo:IAO_0000420 a obo:IAO_0000409 ;
  rdfs:label "defined class"@en ;
  rdfs:comment "A defined class is a class that is defined by a set of logically necessary and sufficient conditions but is not a universal"@en .

obo:IAO_0000421 a obo:IAO_0000409 ;
  rdfs:label "named class expression"@en ;
  rdfs:comment "A named class expression is a logical expression that is given a name. The name can be used in place of the expression."@en .

obo:OBI_0100026 a rdfs:Class ;
  rdfs:label "organism"@en ;
  rdfs:comment "A material entity that is an individual living system, such as animal, plant, bacteria or virus, that is capable of replicating or reproducing, growth and maintenance in the right environment. An organism may be unicellular or made up, like humans, of many billions of cells divided into specialized tissues and organs."@en ;
  rdfs:subClassOf obo:BFO_0000040 .

obo:NCBITaxon_10239 a obo:OBI_0100026 ;
  rdfs:label "Viruses" .

obo:NCBITaxon_2 a obo:OBI_0100026 ;
  rdfs:label "Bacteria" .

obo:NCBITaxon_2157 a obo:OBI_0100026 ;
  rdfs:label "Archaea" .

obo:NCBITaxon_2759 a obo:OBI_0100026 ;
  rdfs:label "Eukaryota" .

obo:ECO_0000217 a rdfs:Class ;
  rdfs:label "assertion method" ;
  rdfs:comment "A means by which a statement is made about an entity." .

obo:ECO_0000203 a obo:ECO_0000217 ;
  rdfs:label "automatic assertion" ;
  rdfs:comment "An assertion method that does not involve human review." .

obo:ECO_0000218 a obo:ECO_0000217 ;
  rdfs:label "manual assertion" ;
  rdfs:comment "An assertion method that involves human review." .

obo:IAO_0000225 a rdfs:Class ;
  rdfs:label "obsolescence reason specification"@en ;
  rdfs:comment "The reason for which a term has been deprecated. The allowed values come from an enumerated list of predefined terms. See the specification of these instances for more detailed definitions of each enumerated value."@en ;
  rdfs:subClassOf obo:IAO_0000102 .

obo:IAO_0000103 a obo:IAO_0000225 ;
  rdfs:label "failed exploratory term"@en ;
  rdfs:comment "The term was used in an attempt to structure part of the ontology but in retrospect failed to do a good job"@en .

obo:IAO_0000226 a obo:IAO_0000225 ;
  rdfs:label "placeholder removed"@en .

obo:IAO_0000227 a obo:IAO_0000225 ;
  rdfs:label "terms merged"@en .

obo:IAO_0000228 a obo:IAO_0000225 ;
  rdfs:label "term imported"@en .

obo:IAO_0000229 a obo:IAO_0000225 ;
  rdfs:label "term split"@en .

obo:IAO_0000078 a rdfs:Class ;
  rdfs:label "curation status specification"@en ;
  rdfs:comment "The curation status of the term. The allowed values come from an enumerated list of predefined terms. See the specification of these instances for more detailed definitions of each enumerated value."@en ;
  rdfs:subClassOf obo:IAO_0000102 .

obo:IAO_0000002 a obo:IAO_0000078 ;
  rdfs:label "example to be eventually removed"@en .

obo:IAO_0000120 a obo:IAO_0000078 ;
  rdfs:label "metadata complete"@en ;
  rdfs:comment "Class has all its metadata, but is either not guaranteed to be in its final location in the asserted IS_A hierarchy or refers to another class that is not complete."@en .

obo:IAO_0000121 a obo:IAO_0000078 ;
  rdfs:label "organizational term"@en ;
  rdfs:comment "Term created to ease viewing/sort terms for development purpose, and will not be included in a release"@en .

obo:IAO_0000122 a obo:IAO_0000078 ;
  rdfs:label "ready for release"@en ;
  rdfs:comment "Class has undergone final review, is ready for use, and will be included in the next release. Any class lacking \"ready_for_release\" should be considered likely to change place in hierarchy, have its definition refined, or be obsoleted in the next release.  Those classes deemed \"ready_for_release\" will also derived from a chain of ancestor classes that are also \"ready_for_release.\""@en .

obo:IAO_0000123 a obo:IAO_0000078 ;
  rdfs:label "metadata incomplete"@en ;
  rdfs:comment "Class is being worked on; however, the metadata (including definition) are not complete or sufficiently clear to the branch editors."@en .

obo:IAO_0000124 a obo:IAO_0000078 ;
  rdfs:label "uncurated"@en ;
  rdfs:comment "Nothing done yet beyond assigning a unique class ID and proposing a preferred term."@en .

obo:IAO_0000125 a obo:IAO_0000078 ;
  rdfs:label "pending final vetting"@en ;
  rdfs:comment "All definitions, placement in the asserted IS_A hierarchy and required minimal metadata are complete. The class is awaiting a final review by someone other than the term editor."@en .

obo:IAO_0000423 a obo:IAO_0000078 ;
  rdfs:label "to be replaced with external ontology term"@en ;
  rdfs:comment "Terms with this status should eventually replaced with a term from another ontology."@en .

obo:IAO_0000428 a obo:IAO_0000078 ;
  rdfs:label "requires discussion"@en ;
  rdfs:comment "A term that is metadata complete, has been reviewed, and problems have been identified that require discussion before release. Such a term requires editor note(s) to identify the outstanding issues."@en .
